LOCUS       c00105_gnlPro..         4539 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|DIPMAPFE_105.
ACCESSION   c00105_gnlPro..
VERSION     c00105_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-05 13:52:25
            Original ID  :: gnl|Prokka|DIPMAPFE_105
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 4539
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..4539
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[997:3953]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      998..3953
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..4539
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..4539
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             3..929
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Fn3-like"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Glyco_hydro_3_C"
                     /gene_functions="other (smcogs) SMCOG1041:Fibronectin type
                     III-like domain (Score: 88.9; E-value: 6e-28)"
                     /gene_kind="other"
                     /locus_tag="ctg105_1"
                     /sec_met_domain="Fn3-like (E-value: 2.9e-29, bitscore:
                     88.9, seeds: 729, tool: rule-based-clusters)"
                     /sec_met_domain="Glyco_hydro_3_C (E-value: 3.3e-43,
                     bitscore: 135.7, seeds: 116, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="TTDRSKVVTLYDALVRKGLEVNYAEGYDMKSNQLTGLQEALQAAE
                     ASDVIVVAVGERFGQSGEKASKVNIAIPEGQQKLVSELAKTGKPVIALVMCGRPVIFNE
                     IREHADAVLCTWWLGSEAGNAICNVLWGEYNPSAKLPMTFPAHVGQIPIYYQNKSTGRP
                     TALQQTYKASYIDASTEPAYPFGYGLSYTDFGYSDLKLLPGKDAGVHTEVSVKVTNTGK
                     CAGEEVVQLYVQDKVASVTRPIKELKGFRKIHLNAGESKTVAFQITDDALGFYDNEMNY
                     MVEPGDFVFMVGGSSDDVQQITYNLKE"
     CDS             complement(998..1723)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 75.5;
                     E-value: 1.7e-23)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg105_2"
                     /sec_met_domain="Radical_SAM (E-value: 5.5e-32, bitscore:
                     99.2, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MINVHSYESMGTFDGPGLRLVVFLQGCPFRCLYCANPDTIDVKGG
                     TPTTPEEILQMAISQKAFFGKKGGITFSGGEPTLQAEALIPLFKELKANGIHICLDSNG
                     GIWNEKVEELFSLTDLVLLDIKQFNPERHRTLTGRSNEQTLRTAAWLEEHEHPFWLRYV
                     LVPGYSDSEEDIRLLGESLGKYRQIQRVEILPYHRLGVHKYEVMGWDYKLKEVKENTPE
                     QLERAEKLFKEYFTNVITN"
     CDS             complement(1725..3953)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate1"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg105_3"
                     /sec_met_domain="Gly_radical (E-value: 1.7e-47, bitscore:
                     148.2, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate1 (E-value: 0.0, bitscore:
                     1171.1, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MELNKTFTNGLWNKEINVSDFVSKNIAPYTGDASFLQGPTERTKR
                     IWDICLKALEEERNNNGVRSLDNTTVSTITSHKAGYIDKDNELIVGLQTDELLKRSIKP
                     FGGINVVAKACRENGVEVDDKVKDIFTHYRKTHNDGVFDVYTEEIRSFRSLGFLTGLPD
                     NYARGRIIGDYRRLALYGIDRLIEAKQEDLRNLTGPMTEARIRLREEVAEQIKALKDIK
                     VMGEYYGLDLSHPATSAQEAVQWVYMAYLAAIKEQDGAAMSLGNVSSFLDIFIEYDLAH
                     GKINETFAQELIDQFVIKLRMVRHLRMQSYNDIFAGDPTWVTEAIGGRFNDGRVKVTKT
                     SFRFLQTLYNLGPSPEPNLTVLWSPELPEGFKDFCAKVSVDTSSIQYENDNLMREVRNC
                     DDYGIACCVSYQAIGKQIQFFGARANLAKALLLAINGGRCENTGTVMVKGIPVLTGETL
                     KFEEVMANYKKVLTEIARVYNEAMNIIHYMHDKYYYEKAQMAFIDTNPRINLAYGVAGL
                     SIAIDSLSAIKYAKVTARRNEIGLTEGFDTEGSFPCFGNNDDRVDHLGVDLVYYFSEEL
                     KKLPVYKNARPTLSLLTITSNVMYGKKTGATPDGRAKGVAFAPGANPMHGRDKNGAIAS
                     LSSVAKLRYRDSQDGISNTFSIVPKSLGPTPEERVENLVTMMDGYFTKGAHHLNVNVLN
                     REMLEDAMEHPEKYPQLTIRVSGYAVNFVKLSREHQLEVISRSFHERM"
ORIGIN
        1 ccactacgga ccggagcaaa gtggtgacct tgtatgatgc actggtccgg aaaggactgg
       61 aggtgaacta tgctgaaggc tatgatatga agagcaatca attgaccgga ctgcaggaag
      121 cattgcaggc tgctgaggct tcggatgtca ttgttgtggc ggtaggcgag agattcggtc
      181 agagtggcga gaaggcttcc aaagtgaata tagccatacc cgaggggcag cagaagctgg
      241 tgtcggaact ggcaaagact ggaaaacctg tcattgctct tgtcatgtgt gggcgtccgg
      301 taatcttcaa tgagataagg gaacatgctg atgcggtact gtgtacttgg tggctgggga
      361 gtgaggccgg caatgccata tgcaatgtgt tgtggggaga gtataatcct tcggctaaat
      421 tgcccatgac atttcctgca cacgtagggc agattcccat ttattaccag aataagagta
      481 ccggtcgtcc taccgcattg caacagactt ataaggcatc ctatatcgat gcttccactg
      541 agcctgccta tcctttcgga tatgggcttt cgtatacgga ttttggatat tctgatttga
      601 aactgctgcc gggcaaggat gcaggcgtcc atacggaggt gtcggtaaaa gtgactaata
      661 ccggtaagtg tgcaggagag gaagtagtcc agttgtatgt gcaggataag gtagcgtctg
      721 ttacgcgccc gataaaggag ttgaagggct tcaggaaaat tcatctgaat gcgggtgaaa
      781 gcaaaactgt agcttttcag ataactgacg atgcactggg attctatgac aatgagatga
      841 attatatggt agaacctggt gatttcgtct ttatggtggg tggcagtagt gatgatgtac
      901 agcagattac ctataacttg aaagaatagg attcttgtcc tataaatgaa aagcaaggct
      961 gtctggaatc atatccggac agccttgctt ttctgtttta gttggttatc acattcgtga
     1021 aatactcttt gaacagtttt tctgcccgtt ccagttgttc cggagtattt tccttcactt
     1081 ctttcaattt gtaatcccat cccatgactt cgtatttgtg aacgcccaga cggtggtaag
     1141 gaagtatttc cacccgttgt atctgccggt atttgccgag actttcaccc agcaggcgga
     1201 tgtcttcttc cgaatcgctg taaccgggaa ccagtacata acgcagccag aaggggtgtt
     1261 catgttcttc gagccaggca gcggtgcgga gtgtctgttc gttgcttcgt cccgtcagcg
     1321 tacggtggcg ttcgggattg aattgcttga tgtccagcag cacgaggtca gtcaggctaa
     1381 agagctcttc caccttttca ttccagatgc ctccgttact gtccagacag atatggatgc
     1441 cgttcgcctt cagctccttg aaaagaggaa tcagcgcttc tgcttgcagg gtaggctcac
     1501 cgccggagaa agtaatacct cctttcttcc cgaagaatgc cttttggctg atagccattt
     1561 gcagaatctc ttccggagtg gtgggagtgc ctccttttac atcaatcgta tccggattgg
     1621 cacaatacag acaacggaac ggacatcctt gcaggaaaac gacgagccgg agacccggcc
     1681 cgtcgaatgt tcccatactt tcgtaggagt gtacatttat catattacat tctttcgtgg
     1741 aatgaacggc tgatgacttc caactgatgc tcgcggctca acttcacgaa gttcacggca
     1801 tagccggaaa cgcggatggt gagttgcgga tacttttcag gatgttccat ggcatcttcc
     1861 agcatttcgc ggttcagtac gtttacgttc aaatggtggg cacctttggt gaagtagccg
     1921 tccatcatgg ttaccaggtt ctctacacgt tcttccggtg tcgggcccag tgatttagga
     1981 acaatggaga acgtattgct gatgccgtcc tgcgagtcgc ggtagcgcaa tttggctacg
     2041 gagctcaagg aagcgatggc accgttcttg tcacgtccgt gcattgggtt ggcacccgga
     2101 gcaaaggcaa cacctttggc gcgtccgtcc ggagtggcac cggtcttctt gccgtacatc
     2161 acgttggagg tgatagtcag caaggaaagg gtgggacggg cattcttgta gacaggcagc
     2221 tttttcagtt cttcgctgaa atagtaaacg aggtctactc ccaggtggtc cacacggtcg
     2281 tcattattgc cgaagcaggg gaatgagcct tcagtgtcga aaccttcggt caggccgatt
     2341 tcgttgcggc gtgcagtgac tttggcatat ttgatggcgg acagtgagtc tatggcgatg
     2401 gaaaggccgg caacaccgta tgccagattg atgcgtgggt tggtatcgat gaatgccatc
     2461 tgggccttct cataataata cttatcatgc atgtagtgga tgatattcat ggcctcgttg
     2521 tagacacggg ctatttcagt cagtaccttc ttgtagttgg ccataacttc ctcaaacttc
     2581 agtgtctcgc cggtcaatac gggaatacct ttcaccatga cagttccggt gttttcgcaa
     2641 cggccaccgt tgatggcaag cagcagggcc ttggcaaggt tggcacgtgc gccgaagaac
     2701 tggatttgct tgccgatggc ttggtaggat acacagcagg caatgccgta atcgtcgcag
     2761 ttgcgcactt cgcgcatcag gttgtcgttt tcgtactgga tggagctggt atctacggaa
     2821 actttggcgc agaaatcctt gaagccttcc ggcagttcgg gactccacag tacggtcaag
     2881 tttggttccg gagaaggacc gaggttgtat agcgtctgca agaagcggaa agaagtcttg
     2941 gtgactttca cgcgtccgtc attgaagcgt cctccgatgg cttcggtcac ccaagtcggg
     3001 tcaccggcaa agatatcatt gtacgactgc atacgcagat ggcgtaccat gcgcagcttg
     3061 atgacgaact ggtctatcag ttcttgcgca aacgtctcat tgatttttcc gtgagccaga
     3121 tcgtattcaa tgaatatatc gaggaacgaa gatacgttac ccaatgacat ggcagcaccg
     3181 tcttgctctt tgatggcagc aagatatgcc atgtataccc attgcacggc ctcttgagct
     3241 gatgtagccg gatggctgag gtcgagtccg taatattcgc ccataacctt gatgtccttc
     3301 aaagctttga tttgttccgc cacttcttcg cgtagacgga tgcgtgcttc ggtcatcggg
     3361 ccggtgaggt tgcgcaagtc ctcctgcttg gcttcaatca gacggtcgat gccgtagaga
     3421 gccaatcggc ggtagtcgcc gatgatacgt ccgcgggcat agttgtcggg caatccggta
     3481 aggaatccca gtgagcggaa ggaacggatt tcttcggtat atacatcgaa tacgccgtcg
     3541 ttatgtgttt tgcggtaatg ggtgaagatg tcttttactt tatcgtccac ttccactccg
     3601 ttttcacggc atgcttttgc taccacgttg atgcctccga aaggtttgat ggatcgtttc
     3661 aacagttcat cagtctgcaa accgacaata agttcattgt ccttatcaat atacccggct
     3721 ttatgagaag tgatggtaga tacggtggta ttgtccagtg aacggacgcc attgttgttc
     3781 ctttcttctt cgagagcctt cagacagatg tcccagatac gtttggtacg ttcggtaggt
     3841 ccctgcaaga aagatgcatc cccggtatag ggtgcgatgt ttttactgac gaaatcactg
     3901 acattgattt ctttattcca aagcccattg gtaaaggttt tgttcaattc cataaaattt
     3961 gtttttgtta gtaggttaga taatgttatc ctttagagcg cacaaaaata ctgaataaat
     4021 tccgtaattt tataagagat acttctcttt ttatgttgaa cagcataaaa taactattat
     4081 taggtagtga atgtccgggg gagtagagat gttaatactt tctatatgtg gtttatgaat
     4141 tgaaacaaga accgaataat aatctgcatt atttacaaat gatgtaggtt taaggaacag
     4201 tcgtattctg aacctaattc agtcgtatag tgaatgccat tcagtcgtat actgaatagg
     4261 gttcaatcgt atactgaatg tggttctttg taaatatact gatagccagt aagttataaa
     4321 actctgtctt gcccttgata aatttcaaca aatagcctaa atgccgttat gttcgttccg
     4381 tagtgggtat aaacaagaat tttggtataa ttgctctgtt tttgtgcaga aaagtttgta
     4441 gaataaaaaa tgatttgtat ctttgcaccg ctttaaaaga aaagcacttc tgaaaaagaa
     4501 gttttggaga ggtggcagag tggtcgattg cggcggtct
//
