LOCUS       c00269_gnlPro..         3811 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|HPGFBCAH_269.
ACCESSION   c00269_gnlPro..
VERSION     c00269_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-07 20:13:48
            Original ID  :: gnl|Prokka|HPGFBCAH_269
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 3811
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..3811
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[1:1697]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      2..1697
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..3811
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..3811
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             2..970
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate1"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg269_1"
                     /sec_met_domain="Gly_radical (E-value: 3.9e-48, bitscore:
                     150.2, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate1 (E-value: 3.8e-104, bitscore:
                     336.7, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="GARTNLAKALLLAINGGRCENTGTVMVKGIPVLTGETLKFEEVMA
                     NYKKVLTEIARVYNEAMNIIHYMHDKYYYEKAQMAFIDTNPRINLAYGVAGLSIAIDSL
                     SAIKYAKVTARRNEIGLTEGFDTEGSFPCFGNNDDRVDHLGVDLVYYFSEELKKLPVYK
                     NARPTLSLLTITSNVMYGKKTGATPDGRAKGVAFAPGANPMHGRDKNGAIASLSSVAKL
                     RYRDSQDGISNTFSIVPKSLGPTPEERVENLVTMMDGYFTKGAHHLNVNVLNREMLEDA
                     MEHPEKYPQLTIRVSGYAVNFVKLSREHQLEVISRSFHERM"
     CDS             972..1697
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 75.5;
                     E-value: 1.7e-23)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg269_2"
                     /sec_met_domain="Radical_SAM (E-value: 5.5e-32, bitscore:
                     99.2, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MINVHSYESMGTFDGPGLRLVVFLQGCPFRCLYCANPDTIDVKGG
                     TPTTPEEILQMAISQKAFFGKKGGITFSGGEPTLQAEALIPLFKELKANGIHICLDSNG
                     GIWNEKVEELFSLTDLVLLDIKQFNPERHRTLTGRSNEQTLRTAAWLEEHEHPFWLRYV
                     LVPGYSDSEEDIRLLGESLGKYRQIQRVEILPYHRLGVHKYEVMGWDYKLKEVKENTPE
                     QLERAEKLFKEYFTNVITN"
     CDS             complement(1766..3811)
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Fn3-like"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Glyco_hydro_3"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Glyco_hydro_3_C"
                     /gene_functions="other (smcogs) SMCOG1041:Fibronectin type
                     III-like domain (Score: 87.2; E-value: 2e-27)"
                     /gene_kind="other"
                     /locus_tag="ctg269_3"
                     /sec_met_domain="Fn3-like (E-value: 9.8e-29, bitscore:
                     87.2, seeds: 729, tool: rule-based-clusters)"
                     /sec_met_domain="Glyco_hydro_3 (E-value: 6.8e-72, bitscore:
                     230.1, seeds: 11, tool: rule-based-clusters)"
                     /sec_met_domain="Glyco_hydro_3_C (E-value: 1.7e-53,
                     bitscore: 169.3, seeds: 116, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="NFTGPDGGNIPKEEYVRQGRIGSMLNVIGVKDIRRYQELALQSRL
                     RIPLIFGLDVIHGYRTGFPLPLAEAASFDLAMIEEAARYNALESAADGLNWVFAPMVDI
                     SWDARWGRVMEGAGEDPYYGSRVAEARVRGLQGDDLADTTTVMACMKHFAGYGAPIAGK
                     EYNSVDMSMGHFANFYMPPYKAAIKVGAATVMSAFNDFNNIPCTANDFLLNRLLREQWG
                     FKGFVVSDYNSVEELVNHRYAVDKKDAAAKALNAGLDMEMVSTCYLTYLKELVQEGKVK
                     ESVLDDAVRRILEKKYELGLFEDPFRYCNPARAARILNAPEVRNASLRMAERSVVLLEN
                     RESVLPLTAQTRKIALIGGLSKSQYDMAGAWAATTDRSKVVTLYDALVRKGLEVNYAEG
                     YDMKSNQLTGLQEALQAAEASDVIVVAVGERFGQSGEKASKVNIAIPEGQQKLVSELAK
                     TGKPVIALVMCGRPVIFNEIREHADAVLCTWWLGSEAGNAICNVLWGEYNPSAKLPMTF
                     PAHVGQIPIYYQNKSTGRPTALQQTYKASYIDASTEPAYPFGYGLSYTDFGYSDLKLLP
                     GKDAGVHTEVSVKVTNTGKCAGEEVVQLYVQDKVASVTRPIKELKGFRKIHLNAGESKT
                     VAFQITDDALGFYDNEMNYMVEPGDFVFMVGGSSDDVQQITYNLKE"
ORIGIN
        1 cggcgcacgt accaaccttg ccaaggccct gctgcttgcc atcaacggtg gccgttgcga
       61 aaacaccgga actgtcatgg tgaaaggtat tcccgtattg accggcgaga cactgaagtt
      121 tgaggaagtt atggccaact acaagaaggt actgactgaa atagcccgtg tctacaacga
      181 ggccatgaat atcatccact acatgcatga taagtattat tatgagaagg cccagatggc
      241 attcatcgat accaacccac gcatcaatct ggcatacggt gttgccggcc tttccatcgc
      301 catagactca ctgtccgcca tcaaatatgc caaagtcact gcacgccgca acgaaatcgg
      361 cctgaccgaa ggtttcgaca ctgaaggctc attcccctgc ttcggcaata atgacgaccg
      421 tgtggaccac ctgggagtag acctcgttta ctatttcagc gaagaactga aaaagctgcc
      481 tgtctacaag aatgcccgtc ccaccctttc cttgctgact atcacctcca acgtgatgta
      541 cggcaagaag accggtgcca ctccggacgg acgcgccaaa ggtgttgcct ttgctccggg
      601 tgccaaccca atgcacggac gtgacaagaa cggtgccatc gcttccttga gctccgtagc
      661 caaattgcgc taccgcgact cgcaggacgg catcagcaat acgttctcca ttgttcctaa
      721 atcactgggc ccgacaccgg aagaacgtgt agagaacctg gtaaccatga tggacggcta
      781 cttcaccaaa ggtgcccacc atttgaacgt aaacgtactg aaccgcgaaa tgctggaaga
      841 tgccatggaa catcctgaaa agtatccgca actcaccatc cgcgtttccg gctatgccgt
      901 gaacttcgtg aagttgagcc gcgagcatca gttggaagtc atcagccgtt cattccacga
      961 aagaatgtaa tatgataaac gtacactcct acgaaagtat gggaacattc gacgggccgg
     1021 gtctccggct cgtcgttttc ctgcaaggat gtccgttccg ttgtctgtat tgtgccaatc
     1081 cggatacgat tgatgtaaaa ggaggcactc ccaccactcc ggaagagatt ctgcaaatgg
     1141 ctatcagcca aaaggcattc ttcgggaaga aaggaggtat tactttctcc ggcggtgagc
     1201 ctaccctgca agcagaagcg ctgattcctc ttttcaagga gctgaaggcg aacggcatcc
     1261 atatctgtct ggacagtaac ggaggcatct ggaatgaaaa ggtggaagag ctctttagcc
     1321 tgactgacct cgtgctgctg gacatcaagc aattcaatcc cgaacgccac cgtacgctga
     1381 cgggacgaag caacgaacag acactccgca ccgctgcctg gctcgaagaa catgaacacc
     1441 ccttctggct gcgttatgta ctggttcccg gttacagcga ttcggaagaa gacatccgcc
     1501 tgctgggtga aagtctcggc aaataccggc agatacaacg ggtggaaata cttccttacc
     1561 accgtctggg cgttcacaaa tacgaagtca tgggatggga ttacaaattg aaagaagtga
     1621 aggaaaatac tccggaacaa ctggaacggg cagaaaaact gttcaaagag tatttcacga
     1681 atgtgataac caactaaaac agaaaagcaa ggctgtccgg atatgattcc agacagcctt
     1741 gcttttcatt tataggacaa gaatcctatt ctttcaagtt ataggtaatc tgctgtacat
     1801 catcactact gccacccacc ataaagacga aatcaccagg ttctaccata taattcatct
     1861 cattgtcata gaatcccagt gcatcgtcag ttatctgaaa agctacagtt ttgctttcac
     1921 ccgcattcag atgaattttc ctgaagccct tcaactcctt tatcgggcgc gtaacagacg
     1981 ctaccttatc ctgcacatac aactggacta cttcctctcc tgcacactta ccggtattag
     2041 tcacttttac cgacacctcc gtatggacgc ctgcatcctt gcccggcagc agtttcaaat
     2101 cagaatatcc aaaatccgta tacgaaagcc catatccgaa aggataggca ggctcagtgg
     2161 aagcatcgat ataggatgcc ttataagtct gttgcaatgc ggtaggacga ccggtactct
     2221 tattctggta ataaatggga atctgcccta cgtgtgcagg aaatgtcatg ggcaatttag
     2281 ccgaaggatt atactctccc cacaacacat tgcatatggc attgccggcc tcactcccca
     2341 gccaccaagt acacagtacc gcatcagcat gttcccttat ctcattgaag attaccggac
     2401 gcccacacat gacaagagca atgacaggtt ttccagtctt tgccagttcc gacaccagct
     2461 tctgctgccc ctcgggtatg gctatattca ctttggaagc cttctcgcca ctctgaccga
     2521 atctctcgcc taccgccaca acaatgacat ccgaagcctc agcagcctgc aatgcttcct
     2581 gcagtccggt caattgattg ctcttcatat catagccttc agcatagttc acctccagtc
     2641 ctttccggac cagtgcatca tacaaggtca ccactttgct ccggtccgta gtggcggccc
     2701 aagcgcctgc catatcgtat tgggattttg agagtcctcc tatcaaggct atcttcctcg
     2761 tctgtgccgt caacggaagc acagattccc tattctccag caatacaacg gaacgctcgg
     2821 ccatacggag cgaagcatta cggacttcgg gcgcattgag tattctggcc gctcttgccg
     2881 gattacagta gcgaaaaggg tcctcaaaca atcccaactc atatttcttc tccaatatac
     2941 gccgtactgc atcatcaagc acactctcct ttactttccc ttcttgaaca agctccttca
     3001 gataagtcaa gtagcaggta cttaccatct ccatgtccag accagcattc agcgctttgg
     3061 ctgcggcatc tttcttgtca acagcataac ggtggttcac caattcctct accgaattat
     3121 aatcggacac aacaaatccc ttaaatcccc actgctctct caatagccgg ttgagcaaga
     3181 aatcattggc agtacaagga atattattaa aatcattgaa agcactcatc acggtagctg
     3241 ctcctacctt tatggccgcc ttatagggag gcatataaaa attggcaaag tggcccattg
     3301 acatatccac actattatac tcctttcctg ctatcggtgc accatatccg gcaaaatgtt
     3361 tcatacatgc catgacagtc gtcgtgtctg ccaaatcgtc tccttgcaag cctctgacac
     3421 gcgcctccgc tacccgagaa ccataataag ggtcctcacc ggccccttcc atgacgcgtc
     3481 cccaacgtgc gtcccacgaa atatccacca tcggggcaaa aacccagttc aaaccatcgg
     3541 cggcactttc caaagcattg tagcgtgcag cttcctcaat catggccaaa tcgaaacttg
     3601 cagcctcggc aagcggaagc ggaaatccgg tacgatagcc gtggataaca tccagtccga
     3661 aaattaaagg aatgcgcaaa cgcgattgca aagcaagctc ttgataacgg cgtatatcct
     3721 ttactcctat cacattcagc atagagccta ttctgccttg ccggacatac tcttctttag
     3781 gaatatttcc accgtcgggt ccggtaaaat t
//
