LOCUS       c00032_gnlPro..         7320 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|DGIBHAJF_32.
ACCESSION   c00032_gnlPro..
VERSION     c00032_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-05 14:14:13
            Original ID  :: gnl|Prokka|DGIBHAJF_32
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 7320
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     CDS             1..825
                     /locus_tag="ctg32_1"
                     /transl_table=11
                     /translation="EESRFLPLIPIYGSQYKMNELVPPAMKLWAANGQTPVVLVRTGWE
                     NGEGFYLGIKGGTASTSHAHMDAGSFVFDALGVRWVQDLGMQEYYSLEKENVDLWNMSQ
                     NGQRWNVFRYNNLAHNTLTVNGKYHDVNGFVPVTNIYSEAHKLGASLNMTDLFGGELEN
                     AVREVALINEKYLSVIDRVKAGSQPATVRWTIVTGAIPRVLDSHMVELTKDGKCMQLIV
                     DSPSVTSVSVLDNVSKNSYDASNEGTCRIVFDVSLESGQESDIKVRLVPAAD"
     protocluster    1..7320
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[2464:5950]"
                     /cutoff="10000"
                     /detection_rule="(Succ_DH_flav_C and FAD_binding_2 and
                     (Fer2_3 or Fer4_7 or SucD_FumR))"
                     /neighbourhood="10000"
                     /product="Fumarate2succinate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      2465..5950
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="10000"
                     /detection_rule="(Succ_DH_flav_C and FAD_binding_2 and
                     (Fer2_3 or Fer4_7 or SucD_FumR))"
                     /neighbourhood="10000"
                     /product="Fumarate2succinate"
                     /protocluster_number="1"
     cand_cluster    1..7320
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(Succ_DH_flav_C and FAD_binding_2 and
                     (Fer2_3 or Fer4_7 or SucD_FumR))"
                     /kind="single"
                     /product="Fumarate2succinate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..7320
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Fumarate2succinate"
                     /region_number="1"
                     /rules="(Succ_DH_flav_C and FAD_binding_2 and (Fer2_3 or
                     Fer4_7 or SucD_FumR))"
                     /tool="gutsmash"
     CDS             complement(829..2076)
                     /locus_tag="ctg32_2"
                     /transl_table=11
                     /translation="MLRVPVPLFAISAITYGQGDKFRTLRNTHTPNFHYRYDDFLQYTP
                     LLAVYGMKLGGVESRSSWPRMIVSNAFSATIMAATVNSMKYTIKRERPDGSKNNSFPSG
                     HTATAFMAATIMHREYGLTRSPLYSIGGYTVATATAFSRQLNNRHWLSDVLAGAGIGIL
                     STELGYFLADLIFKDKGLNLPDKLRESAPKGGRPSFLEFAVGYSIINGSIEVGKNIRLS
                     SPGATNVSVKGGYFFNPYFGIGGEITALASPMAFDMSLYTGTTPSTKYDITRVHADPTG
                     VFTFTAGPYFSLPVASKLLLGTKFQAGYSYLVKNEVEMDLQEKPPSYGTINNIVFMKGN
                     GSSNYVLRTGISATGIVSRNLGIRMFVDYDYTFLKAPYEILTSLRDSQPVYGESHLSKH
                     KIHYFTIGATVTALFW"
     CDS             2465..3190
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Fumarate2succinate: SucD_FumR"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg32_3"
                     /sec_met_domain="SucD_FumR (E-value: 1.2e-76, bitscore:
                     245.5, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MWLLNSSIGKKLIMSVSGLFLVLFLLFHLSMNVAAAFSGEAYNMI
                     CSLLGANWYAVAATLVLAAGVVIHFVYAVLLTLQNRKARGNDRYAINARPKGVEWASQN
                     MLVLGIIIILFMVLHFSQFWYKMMFAELLGNHMVTLCDGTTTVSPQDGAAFINYYFQGN
                     ILNTVLYLVWYVALWFHLTHGFWSAIQTIGWSNTIWMNRWICISKIVATVICVVFAIIT
                     IVFYLNGVGGDYVAVCAGH"
     CDS             3224..5167
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Fumarate2succinate: FAD_binding_2"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Fumarate2succinate: Succ_DH_flav_C"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1027:FAD binding domain (Score: 195.7; E-value:
                     4.7e-60)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg32_4"
                     /sec_met_domain="FAD_binding_2 (E-value: 5.3e-61, bitscore:
                     195.7, seeds: 62, tool: rule-based-clusters)"
                     /sec_met_domain="Succ_DH_flav_C (E-value: 5.3e-39,
                     bitscore: 122.1, seeds: 739, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTQIDSKIPQGPLAEKWKNYKDHQKLVNPANKRRLDVIVVGTGLA
                     GASAAASLAEMGFNVLNFCIQDSPRRAHSIAAQGGINAAKNYQNDGDSVYRLFYDTIKG
                     GDYRAREANVYRLAEVSNSIIDQCVAQGVPFAREYGGLLDNRSFGGAQVSRTFYARGQT
                     GQQLLLGAYSALSRMIGLNKVKMYTRHEMLDVVKVDGRARGIIARNLITGQIERFSAHA
                     VVVATGGYVNTFFLSTNAMASNGSAAWQCYKKGAYFANPCMVQIHPTCVPVKGDYQSKL
                     TLMSESLRNDGRIWVPKKLEDAKALQAGTKKGKDIPEADRDYYLERRYPAFGNLVPRDV
                     ASRAAKERCDAGFGVNNTGLAVFLDFSDAINRLGKDVVKAKYGNLFDMYEEITNDDPYE
                     TPMMIYPALHYSMGGLWVDYELMTSIPGLFAIGEANFSDHGANRLGASALMQGLADGYF
                     VLPYTIQNYLSDQIQVPRFSTDLPEFAEAEKGIKDRIKKLMSIKGKESVDSIHKKLGHI
                     MWEHVGMARDAKGLQEAIQMLKELKKEFWSNVYIPGEADNLNVELDKALRLADFIEIGT
                     LMAHDALDRAESCGGHFRTEHQTEEGEALRHDDKFMYVSCWEYQGEDKDPVMLKEDLNY
                     QFVVPQTRNYKK"
     CDS             5195..5950
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Fumarate2succinate: Fer2_3"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Fer4_8"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Fer4_4"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg32_5"
                     /sec_met_domain="Fer2_3 (E-value: 4.7e-27, bitscore: 83.2,
                     seeds: 38, tool: rule-based-clusters)"
                     /sec_met_domain="Fer4_8 (E-value: 1.6e-12, bitscore: 36.8,
                     seeds: 153, tool: rule-based-clusters)"
                     /sec_met_domain="Fer4_4 (E-value: 1.7e-06, bitscore: 17.5,
                     seeds: 79, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDKDIKVTLKVWRQKSPKDKGQFETYQIDKINQSVSFLEMLDILN
                     ERLVSEGKEPIVFDHDCREGICGMCSLYVNGHPHGPATGATTCQLYMRRFNDGETITIE
                     PWRSAGFPVIRDLMVDRYAYDKIIQAGGFVSVNTGGVPDANSIAIPRADAEMAMDAAAC
                     IGCGACAAACKNGSAMLFVSAKVSQLALLPQGKVEAARRAKAMVAKMDELGFGNCTNTR
                     ACEMECPKNISVSNIARLNREFICAKLQD"
     CDS             complement(6249..7094)
                     /locus_tag="ctg32_6"
                     /transl_table=11
                     /translation="MEDNIPKFDLPVDFIVGNSITGEILNQYGRFPCKIKAGIFVLCVQ
                     GTVHATVNLTEFTIRKNDFITLPPGCFIQIHEVSDDVKLCFAGFSSVFVSHINYIKTIT
                     NYLPVIFDSPIMPLPEHISLLYQQAFDLLINAYSMPKTIENKEIIKAIFTIFMQGVIEL
                     YRNHTPYSNAPMTRNMEICREFVQLAMENYTKEHSVSYYAKRLNITLQHFCYVIKKVSG
                     RTALDILSNIIIMDAKAQLKSTDMPVKKIAFALGFDNLSFFNKYFRQHVGMTPQEYRES
                     "
     CDS             7186..7320
                     /locus_tag="ctg32_7"
                     /transl_table=11
                     /translation="MEIVLCCIIVEICECFQVLLIDLLGWYIFVYLLNKIWELILIEKS
                     "
ORIGIN
        1 gaggagagcc gtttccttcc gttgattccg atttatggtt cacaatataa aatgaatgaa
       61 ctggtacctc ctgccatgaa attgtgggcg gctaacggac agacgcctgt cgtgctggtc
      121 cgtacgggtt gggagaatgg agaaggtttc tatctgggaa taaaaggagg aacggcttcg
      181 accagccatg cccatatgga tgccggatcg tttgtgttcg atgctttggg tgttcgctgg
      241 gtgcaggacc tggggatgca ggaatattat tcgctggaaa aggaaaatgt agatttatgg
      301 aatatgtcgc agaacgggca gcgctggaat gtgttccgtt ataataacct ggcgcataat
      361 acactgactg taaacggtaa ataccatgat gtaaacggtt ttgtacctgt gacaaatatc
      421 tattcggaag cgcataaact cggagcttct ctgaatatga cggacttgtt cggcggagaa
      481 ttggaaaatg ccgtccgtga agttgctctg atcaatgaaa aatatctgtc ggtgattgat
      541 cgtgtaaaag ctggcagtca gccggcaact gtcagatgga cgattgtaac cggggccatt
      601 ccccgtgtat tggacagtca tatggtggaa ctgacaaaag acggcaaatg tatgcaactg
      661 atcgttgata gtccgtccgt aacttctgtg tccgtccttg ataatgtatc gaagaatagc
      721 tatgatgctt ccaatgaggg aacctgccgt atcgttttcg atgtgagtct ggaaagcggg
      781 caggagagcg atatcaaagt ccggctggtt cctgctgctg actgataatt accagaacag
      841 tgccgttacg gttgcgccaa tcgtaaagta gtgtattttg tgcttgctta aatggctctc
      901 gccgtagact ggttggctgt ctctgaggct ggttaagatt tcgtaaggtg ctttcaggaa
      961 cgtataatcg taatctacga acatacggat acccaggttg cggctgacga tcccggtcgc
     1021 tgaaatgcct gtgcgtaaaa catagttgct gctaccgttg cctttcataa aaacaatatt
     1081 atttatggtt ccgtatgagg ggggcttttc ctgtaagtcc atttccactt catttttcac
     1141 caggtaggaa tatccggcct ggaactttgt tcctaaaagc agcttgcttg ctactggtaa
     1201 cgaaaaataa ggccctgccg taaaagtaaa aacaccggtc ggatcggcat ggacgcgggt
     1261 gatgtcatat ttggttgaag gggttgttcc tgtataaagc gacatgtcga atgccatcgg
     1321 gcttgccagc gctgttattt cgccgccgat accgaaataa ggattgaaga agtaaccgcc
     1381 ttttacgctg acattggttg ctcccggcga gctcaggcgt atatttttac ctacctcgat
     1441 acttccgttg atgatactgt atcctacggc gaactccagg aaagaagggc gccctccttt
     1501 gggggcagac tccctgagct tgtcagggag attcaagcct ttgtccttga agatcaggtc
     1561 tgccagaaaa taccctaact ctgtggataa gatgccgata ccggccccgg ccagtacgtc
     1621 gctcagccag tggcggttgt tcagttgccg gctgaaagcg gtggctgtgg cgactgtata
     1681 tcccccgata ctatataacg ggctgcgggt cagaccatat tcccggtgca tgatggtagc
     1741 agccatgaaa gcggttgccg tgtgtcccga agggaatgag ttgttctttg agccgtcggg
     1801 gcgttcgcgt ttgatcgtat atttcatgct gttgacggta gcggccatga tcgttgccga
     1861 aaaagcgttc gatacgatca tccggggcca tgaactacga ctttccacac cgccgagctt
     1921 catcccgtaa acagcaagca acggagtata ttgcagaaaa tcatcgtatc tgtaatggaa
     1981 gttgggcgta tgcgtattgc gcaatgtccg gaatttatca ccctgcccat atgttatggc
     2041 tgaaatggcg aaaagaggaa cagggacacg tagcatctgg tataccttat tctctgatat
     2101 tttgttgtaa gtggtttcaa attcatgttc tttatttatg aatctcgtac tatcttttca
     2161 ctgagcacga acagacggta taaaaatcat cataaataag aagctcagca ttttacgttg
     2221 taacatacga tcccctgatt atgccacgaa gatataaaaa tgccggttag tactggttac
     2281 atttttgtta atttatttat tgaataggat ttaccgtttg tccctcctga aatgtatagg
     2341 tttgattgac gaaatatttc tttaatcgtt tgcgtgatac gtgtatttag catacctttg
     2401 cctgataatt gagtatattg atatgaattt ctaaatacaa ttaaataact taaattatta
     2461 tcttatgtgg ttacttaatt cttctatcgg gaagaagctg ataatgagtg tatcaggcct
     2521 ttttcttgtt ttgttcttat tgttccactt gtcaatgaat gtggcggctg ctttctcggg
     2581 agaagcctat aacatgattt gttcattgtt gggagcaaat tggtatgccg tggcagcaac
     2641 attggtgctg gcagcaggcg ttgtgattca ctttgtgtat gctgttctcc ttacactgca
     2701 aaaccgtaag gctcgcggta acgaccgtta tgcaattaac gctcgtccga aaggtgtgga
     2761 atgggcatct cagaacatgc tggtactggg tattatcatt atcctgttca tggtgttgca
     2821 cttcagccag ttctggtata agatgatgtt cgccgaactc ctaggcaatc acatggtaac
     2881 actttgcgac ggaacaacaa ccgtatctcc tcaggacggt gcagcgttta tcaactacta
     2941 tttccagggt aatatcctga atacagtgct gtatctggta tggtacgtag ctttgtggtt
     3001 ccacctgact cacgggttct ggagcgctat tcagactatc ggttggagta acacgatctg
     3061 gatgaaccgt tggatctgca tttccaagat tgtagcaacc gttatttgcg tagtgtttgc
     3121 tatcattact atcgttttct acctgaatgg tgtaggtggt gactatgtag ctgtttgtgc
     3181 aggacattaa tttaaggtgt aacaatattg aaaataaatc attatgactc aaatagattc
     3241 taaaatccct cagggcccgt tggctgagaa gtggaaaaat tataaagatc atcagaaact
     3301 ggtgaacccc gcgaacaaac gtcgtctgga cgtgattgta gttggtactg gtctggccgg
     3361 tgcttctgct gctgcctctc tggcagaaat gggattcaac gtattgaact tctgtatcca
     3421 ggattctccc cgtcgcgcac actcaatcgc cgcacagggt ggtatcaatg ctgcaaagaa
     3481 ttatcagaat gatggtgact ctgtttaccg tctgttctac gatacaatca aaggtggtga
     3541 ctatcgtgcc cgcgaagcaa acgtttatcg gctggctgaa gtttccaact ctattatcga
     3601 ccagtgcgta gcacagggtg ttcctttcgc tcgcgaatac ggtggtctgc tggataaccg
     3661 ttcattcggt ggtgctcagg tatcccgtac gttctatgcc cgcggtcaga ccggacagca
     3721 gttgttgctg ggtgcttact ctgctttgag ccgtatgatc ggtttgaaca aagtaaagat
     3781 gtatacccgc cacgaaatgc tggacgttgt gaaggtggat ggccgtgccc gcggtatcat
     3841 cgcacgtaac ctgatcacag gtcagatcga acgtttctct gctcacgcag tagtggtggc
     3901 aacaggtggt tatgtaaata ctttcttcct gtctactaac gcaatggctt ccaacggttc
     3961 tgctgcatgg cagtgctaca agaaaggtgc ttacttcgct aacccttgta tggtgcagat
     4021 ccacccgact tgtgtaccgg tgaaaggtga ctaccagtct aagctgactt tgatgtctga
     4081 atcattgcgt aacgacggtc gtatctgggt tccgaagaaa ctggaagatg caaaagcatt
     4141 acaggccgga actaagaaag ggaaagacat tcctgaagca gaccgcgact actatctgga
     4201 acgccgttat ccggcattcg gtaacctggt tccgcgtgac gtggcttcac gtgccgctaa
     4261 agaacgttgc gacgccggct tcggtgtgaa caacacaggt ctggccgtgt tcctcgactt
     4321 ctccgacgcc atcaaccgtc tgggtaaaga tgttgtaaaa gctaaatatg gtaacttgtt
     4381 cgacatgtac gaagaaatta ccaacgacga tccatacgaa actccgatga tgatttatcc
     4441 tgcattgcac tactcgatgg gtggtctgtg ggttgactat gaattgatga cttctattcc
     4501 gggtctgttc gctatcggtg aagctaactt ctctgaccac ggagctaacc gtctgggtgc
     4561 ttctgcgttg atgcagggat tggccgatgg ctactttgta ttgccttata caatccagaa
     4621 ctatctgtca gaccagatcc aggttccgcg tttcagtacc gacctgcctg agtttgcaga
     4681 agctgaaaag gggatcaaag accgcatcaa gaaattgatg agtatcaagg gtaaagagtc
     4741 ggtagattct atccataaga aactgggtca tatcatgtgg gaacatgtag gtatggcacg
     4801 tgatgcaaaa ggtttgcagg aagcgatcca gatgttgaaa gagctgaaga aagagttctg
     4861 gagtaatgta tatattccgg gtgaagccga taacctgaac gtagagttgg ataaggcatt
     4921 gcgtttggct gactttattg agattggtac attgatggca cacgatgcat tggaccgtgc
     4981 cgaatcttgt ggaggacact tccgtacaga gcaccagacc gaggaaggcg aagccctgcg
     5041 tcatgacgat aagttcatgt acgtatcttg ttgggaatac cagggtgaag acaaagatcc
     5101 ggttatgctg aaggaagatt tgaactatca gttcgttgtt cctcaaacac gtaactacaa
     5161 gaagtaataa tcatcaatca caaaaattag aatcatggat aaagatataa aagtaacact
     5221 taaagtttgg cgtcagaaaa gtccgaaaga taaaggacaa tttgagactt accaaataga
     5281 caaaatcaat cagagtgttt ctttcctgga aatgttagac attttgaatg aacgtttggt
     5341 cagtgaaggt aaagagccga tcgtattcga tcacgactgt cgtgaaggta tctgcggtat
     5401 gtgttctttg tatgtaaacg gacatccgca cggacctgca accggcgcaa ctacttgcca
     5461 gctttatatg cgtcgcttca atgatggcga aactattaca atcgaaccgt ggcgttctgc
     5521 cggtttcccg gtaatccgcg acttgatggt agaccgttat gcttacgata aaattatcca
     5581 ggcaggtggt ttcgtatcag tgaacacagg tggtgtacct gatgccaatt caatcgctat
     5641 tcctcgtgca gatgctgaaa tggctatgga tgctgctgca tgtatcggtt gcggtgcttg
     5701 tgctgctgct tgtaagaacg gttcggctat gttgttcgtt tctgcaaagg ttagccagtt
     5761 ggctctgttg cctcagggta aggtagaagc tgctcgtcgt gcgaaagcaa tggttgccaa
     5821 gatggacgaa ctgggcttcg gtaactgtac caacacgcgt gcttgcgaaa tggaatgtcc
     5881 gaagaacatc tctgtcagca acatcgctcg tttgaaccgc gagttcattt gtgctaaatt
     5941 gcaagactaa tatacattat atatgtatgt ccggagaggc gttgcttatg gcggcgcctc
     6001 ttcttttttg cccctatttt aacatttctg aagtcataga taaatatctt gaatgttgtt
     6061 agtgtttctt atatgctata caacactaat tagcatttta actattgaca agttggagaa
     6121 tacctgtatc tttgtactat ggttttttca taatagtatt agatttaagg ttaacaaagt
     6181 tggttagggc ttgtcgtgag acaggcttta attgtttata ggggtttgcg tccggcatat
     6241 cccttttttt agctctccct gtattcctgc ggagtcattc ctacatgttg ccggaaatat
     6301 ttgttgaaga acgaaaggtt gtcgaagccc agggcgaatg caattttctt gaccggcata
     6361 tcggtcgatt taagctgcgc tttggcatcc ataatgatga tattgctcag gatatccaat
     6421 gcagtccgtc cgctgacctt ttttatcaca taacagaaat gctgcaaggt gatgttgagc
     6481 cttttggcgt aataagacac gctatgctct ttcgtgtagt tttccatcgc cagctgtacg
     6541 aactcccggc atatttccat attacgcgtc atgggagcat tgctatatgg cgtatgattc
     6601 ctgtataatt caataacccc ttgcatgaag atagtaaaga tagccttgat aatctccttg
     6661 ttttcgatgg ttttaggcat ggaataagca ttgatcagca agtcgaaagc ctgttggtag
     6721 agtaaggata tatgctccgg caaaggcatg atcggactgt cgaatatgac aggcagatag
     6781 ttcgtaatcg tctttatata attgatatgg ctgacgaata cggaagaaaa gcctgcaaaa
     6841 cacagtttta catcatcgga aacctcatgt atctgaatga agcaacccgg gggcaaggtg
     6901 ataaagtcgt ttttccggat ggtaaactca gttaagttta cagtcgcatg gactgtccct
     6961 tgtacgcaaa gcacgaatat gcctgccttg atcttgcacg ggaaacgtcc gtattggttc
     7021 agtatttcac ctgtaatact gtttcctaca ataaaatcta cggggaggtc gaatttagga
     7081 atattgtctt ccatactttt atatacactt aattgaatgt acaaagatag tttttttgag
     7141 acaagttagt cgataatctt aaatatagaa catcgaatcc cacaaatgga gattgttttg
     7201 tgctgtataa tagttgaaat ttgcgaatgt tttcaagtct tgctgataga tttgttggga
     7261 tggtatatat ttgtctatct gctaaataag atatgggagc taatactaat agagaaatct
//
