LOCUS       c00088_gnlPro..         5280 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|DGIBHAJF_88.
ACCESSION   c00088_gnlPro..
VERSION     c00088_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-05 14:14:13
            Original ID  :: gnl|Prokka|DGIBHAJF_88
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 5280
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..5280
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[316:3293]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      317..3293
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..5280
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..5280
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             317..2557
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate2"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg88_1"
                     /sec_met_domain="Gly_radical (E-value: 1.3e-45, bitscore:
                     142.8, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate2 (E-value: 4.2e-285, bitscore:
                     935.1, seeds: 33, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKFENENWAQFKGEIWKREINVRDFIQNNYKPYEGDDSFLKDSSD
                     KTKKVWDKLTEMFKVEREKGVYDAETKYPQGIDTYGPGYIDKENEVIVGLQTDAPLKRG
                     IYPKCGTRMVENSLEAYGYKLDPMTKEIFTKYRKTHNEGVFSAYTDEMLAARRSAIITG
                     LPDAYGRGRIIGDYRRVALYGTEILIAEKKHYLKRLDIQELTEEIIQSREEISEQIKAL
                     KAFEKMCAGYGFDVTKPAKDAREAVQFVYLGYLAAVKDQDGAAMSLGRTSTFLDIYIEK
                     DIREGKLTEEEAQELIDQFIIKLRIVRFLRTPEYNDLFSGDPVWVTESLGGQGVDGRSL
                     VTKTSYRYLHTLYNLGPAPEPNLTVLWFNNAPENWKRFCAKVSIDTSAIQYENDDLMRP
                     DYGDDYGIACCVSPMKIGKQMQYFGARANLAKCLLYAINGGRDERSGVQVAPRFEPITS
                     EYLDYNEVMEKYEQMMRWLAKVYVNALKIIHYMHDKYAYEAFEMALHDGSVERIRATGI
                     AGLSIVADSLAAIRDTKVKVIRDERGLAVGFEREGDYVPFGNNDDRTDSIAVDITEKFM
                     EYLRQHQTYRNATPTQSILTITSNVVYGKKTGATPDGREGGTPFAPGANPMNGRDTKGA
                     VAALASVAKLPFQHAHDGISYTFAVSPATLGKERDIQIANMVGLLDGYFTPDGGQHLNV
                     NVFDKELLLDAMEHPENYPQLTIRVSGYAVNFVKLTREQQLDVISRTINHSL"
     CDS             2565..3293
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 72.3;
                     E-value: 1.5e-22)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg88_2"
                     /sec_met_domain="Radical_SAM (E-value: 4.6e-37, bitscore:
                     116.4, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="LEIKGNIHSLESFGTVDGPGIRFVVFMQGCPLRCLYCHNPDTWDS
                     KGLAKYQMTPEELLAEVLRYKNFIAKGGVTVTGGEPLLQAEFLREFFRLCREAGIHTAL
                     DTSGYICTPKALAVLEYADLVLLDIKTIDPDLHPRLTAVKLDNTLRFLDELEKRGVPVW
                     IRHVIVPGLTDNDEALDKLAEYISHYTVVQKAELLPYHTMGTYKYEAQGLDYKLKEVEP
                     LSAERLANAKEIFKKHGITI"
     CDS             3382..4284
                     /locus_tag="ctg88_3"
                     /transl_table=11
                     /translation="MEQHHHDHHHHEITSLNKAFIIGISLNLAFVVAEFTAGFWYNSLG
                     LLSDAGHNLGDVASLVLAMLAFRLAKVHPTCSYTYGYKKSTVLVSLLNAVILLVAVGII
                     IAESIGKLLHPRPVEGDAIAWVAGIGVVINALTAWLFMKDKDKDLNVKGAYLHMAADAL
                     VSVGVVVSGIVIAHTGWYIIDPIIGLLIAFVIIVSTWGLLRDSIRLSLDGVPAGTDIDK
                     VEKTIHSVEGVEGVHHLHIWAISTTETALTAHVSVADISEMERIKQEIKHDLQHVGIQH
                     ATLEFETSGFKCDGRCCSD"
     CDS             4445..5011
                     /locus_tag="ctg88_4"
                     /transl_table=11
                     /translation="MEPIINSQAPEFKVQAYHNGSFKTVSSDDIKGKWAIFFFYPADFT
                     FVCPTELVDMADKYDQFQAMGVEVYSVSTDSHFVHKAWHDASETIRKIKYPMLADPTGV
                     LSRAFGVMIEEDGMAYRGTFLVNPEGKIKLAEINDNGIGRNADELLRKVEAAQFIAEHP
                     SEVCPAKWKKGGETLKPSIDLVGKI"
     CDS             5105..5278
                     /locus_tag="ctg88_5"
                     /transl_table=11
                     /translation="MLDSSMRNQLSGIFSGLTNEYIFDIQVSPEHESRKELLELLNDVA
                     SCSDKITVQVADG"
ORIGIN
        1 caaaaccttt tgctaattat gtaagcatat aagatgatta aacgtgtgtc ggaaattttt
       61 tattggttgt ttccggcata aaaaagggac tctcctgcga aggatggttc cttttttatt
      121 tttttgatat tatttataat ccttcctgtt tgatacctat atcaggcgat acgctgcact
      181 atatctaccg ttttagtaac tatttgtagg gattttggcc gttgggaata catgatgttt
      241 ttgttgattg tttcgcatgc catataaata acgtaccttt gcaccgaaat taaaaccgaa
      301 gaaaacaaaa taaggtatga agtttgaaaa cgagaactgg gcgcagttca aaggcgaaat
      361 ttggaaaaga gagattaacg taagggattt tatccagaac aactacaaac cttacgaagg
      421 ggacgactct ttcttgaaag actcctcaga taagacgaag aaagtttggg ataaattgac
      481 cgaaatgttt aaggttgaaa gggagaaggg tgtttacgac gctgaaacaa aatatcctca
      541 aggtatagat acttacggac caggttatat tgacaaagaa aacgaagtaa ttgtcggcct
      601 gcaaaccgat gcccccctga aacgcggtat ttacccgaaa tgtggtaccc gcatggtgga
      661 aaacagtctg gaagcatatg gctacaaact ggacccgatg acaaaagaaa tcttcacgaa
      721 atacagaaag acgcataacg aaggcgtatt ctccgcttat acagatgaaa tgcttgcagc
      781 gcgccgttcc gctatcatca ccggcctgcc ggatgcttac ggacgcggac gtattatcgg
      841 tgactaccgc cgtgtagcac tttacggtac tgaaatcctg atcgctgaaa agaaacatta
      901 tctgaaaaga ctcgatattc aggaactgac cgaagagatc atccagagcc gtgaggaaat
      961 atccgaacag atcaaggcgc tgaaggcttt cgaaaagatg tgtgccggtt atggtttcga
     1021 cgtgaccaag cctgcaaagg atgcacgcga agcggttcag tttgtctatc tgggttatct
     1081 ggctgcggtg aaggaccagg atggggctgc catgtcgttg ggacgtactt ctactttcct
     1141 cgatatttat attgaaaaag atatccgcga aggcaagctg acggaagaag aagctcagga
     1201 gctgatcgat cagtttatta tcaaactgcg tatcgtccgt ttccttcgta ctccggaata
     1261 taacgacctg ttctccggtg accctgtttg ggtaaccgag tctttgggcg gacagggtgt
     1321 cgacggccgt tcattggtga cgaagacgtc ttaccgttat ctgcatacat tatataacct
     1381 cggcccggca ccggaaccca acttaacggt actgtggttc aacaacgctc ctgaaaactg
     1441 gaaacgtttc tgtgcgaagg tttctatcga tacttccgct atccagtatg agaacgacga
     1501 tctgatgcgt ccggattatg gagatgatta tggtattgcc tgctgcgtgt ctccgatgaa
     1561 gatcggtaag cagatgcagt atttcggcgc acgtgccaac ctggctaaat gtttgctgta
     1621 tgccatcaac ggcgggcgcg acgaaagatc aggtgtacag gtggctccga ggtttgaacc
     1681 gatcaccagc gaatacctgg attacaacga agtgatggag aaatacgaac agatgatgcg
     1741 ctggctggct aaagtttatg tgaatgcact gaagatcatc cattacatgc acgataaata
     1801 tgcgtatgaa gcattcgaga tggctttgca tgacggttcg gtagaacgta tccgcgcaac
     1861 aggtattgcc ggcctttcta tcgttgccga ctcattggct gctatccgcg atacgaaagt
     1921 aaaagtgatc cgcgacgaac gtggcctggc tgtcggtttc gaacgtgaag gagattatgt
     1981 tcctttcggt aacaatgacg atcgtacaga tagtattgct gtcgacatca ctgaaaagtt
     2041 catggaatat ctgcgtcagc accagacata ccgcaacgcc actccgacac agtctatcct
     2101 gactatcaca tccaatgtgg tgtatggaaa gaagacaggt gcaactcccg acggtcgcga
     2161 aggtggtacg ccgtttgctc cgggtgcgaa cccgatgaac ggacgtgata ccaaaggcgc
     2221 tgttgctgca ctggcttctg ttgctaaact gccgttccag catgcacacg acggtatctc
     2281 ttatacattc gctgtttctc ctgcaacgtt aggaaaagaa cgtgatattc agatcgccaa
     2341 tatggtcggc ctgctggacg gttactttac tcctgacggc ggacagcatc tgaatgtgaa
     2401 cgtattcgac aaggagctgt tgctcgatgc tatggagcat ccggaaaact atccgcagtt
     2461 gacaatccgc gtatcgggtt atgcagtaaa ctttgtgaaa ctgacacgcg aacagcagct
     2521 ggatgtgatt tcaagaacaa tcaatcattc tttgtaattg aaagttggaa attaaaggaa
     2581 acatacattc actagagagt ttcgggaccg ttgatggtcc cggaattcgt tttgttgtct
     2641 ttatgcaggg ttgtccgctc cgttgcctgt attgccacaa tccggatacc tgggatagca
     2701 aaggtcttgc caaatatcag atgacgcctg aagaattatt ggctgaggtt ttacgatata
     2761 agaactttat cgccaaggga ggcgtgactg tcaccggggg agaaccttta ttgcaagctg
     2821 aatttctcag ggagtttttc cgtctttgcc gggaagccgg tattcatacg gcgctcgata
     2881 catccggcta tatctgcaca ccgaaagccc tggcggttct ggaatatgcc gacctggtct
     2941 tattggatat aaaaaccatc gatcccgacc tgcatccccg tctaacggcc gtgaaactgg
     3001 ataatacgtt acgtttcctg gacgaactgg aaaaacgcgg tgttccggtt tggatacgcc
     3061 atgtcattgt gcccggactg acggataatg acgaagcctt ggataaactg gccgaataca
     3121 tcagccacta tacggttgta cagaaggcgg aactcttgcc ttatcacacg atgggcacat
     3181 ataaatacga ggctcaaggt ctcgactaca aactgaaaga ggtagaacct ttatcggccg
     3241 aacgtctggc aaatgccaaa gaaatattta agaagcacgg tataaccatc tgataattta
     3301 cctataatca ggtatatttt tattgctcta aaaaacatag ttttgtccca tattgttact
     3361 caaataaaag taggaatgaa tatggaacaa catcatcatg atcatcacca ccacgaaata
     3421 acctctttga acaaggcttt cattataggt atctccctga acctggcttt tgtcgtagcc
     3481 gaatttacgg ctggcttctg gtataattca ttaggacttc tgtcggatgc agggcataac
     3541 ctgggagatg tggccagcct ggtactggct atgctggctt tccggctggc taaggtgcac
     3601 ccgacttgca gctataccta tggatataag aaaagcactg tactcgtatc cctcctgaat
     3661 gcggtcatcc tcttggtggc cgtcgggatt attattgctg aaagtatagg aaaattgctg
     3721 catccgcgtc cggtcgaggg tgatgcgatc gcttgggtgg caggtatcgg agtcgttata
     3781 aatgcactta cggcatggct tttcatgaag gataaggata aagacttgaa tgtgaaaggt
     3841 gcttacttgc atatggctgc cgatgcactg gtatcggtcg gcgtggttgt ctccggaatt
     3901 gttattgccc ataccggatg gtatatcatc gacccgatta tcggcctgtt gattgcgttc
     3961 gttatcattg tttctacctg gggactgcta cgcgacagta tccgtctctc tctggacggt
     4021 gtgccggcgg gaacggacat cgataaggtg gaaaagacga tacattcggt cgaaggcgtt
     4081 gaaggcgtcc atcatctcca tatctgggcg atcagtacga ccgaaacggc acttaccgcc
     4141 catgtttccg ttgcggatat ttcagaaatg gaacgtatca aacaggagat aaaacacgac
     4201 cttcagcatg tgggaatcca gcatgctacg ctcgaatttg aaacgtcggg attcaagtgc
     4261 gatgggcgct gttgctcaga ctgaaacagg tatattataa acctgattta ctgatactca
     4321 aagattttgt ctatgactgc ataaaacctt tcgattggat tttttgttct tacctcgaat
     4381 cggcaactta tctttgcacc agtaacaaaa acaaaatagt aataacgatt aaaataaaaa
     4441 ttctatggaa cctattatca attcacaagc acccgagttt aaagttcagg cataccataa
     4501 cggtagtttc aaaactgtaa gcagcgacga catcaaaggc aaatgggcga ttttcttttt
     4561 ctacccggct gactttacat ttgtttgtcc gaccgagttg gtagatatgg ctgacaaata
     4621 tgaccagttc caggcaatgg gtgtagaagt ttactctgta agtacagatt cacacttcgt
     4681 acacaaagca tggcacgatg cttctgaaac gattcgtaag atcaaatacc cgatgttggc
     4741 agacccgaca ggcgtattga gccgtgcatt cggtgtaatg atcgaagaag acggtatggc
     4801 ttaccgcggt acattcctgg taaatccgga aggaaagatc aaactggcag aaatcaacga
     4861 taacggtatc ggccgtaacg ccgacgaact gcttcgtaaa gtagaagctg cccagttcat
     4921 cgccgaacat ccgagcgaag tttgccccgc caaatggaaa aaaggtggcg aaacattgaa
     4981 accgagcatc gatctggtcg gtaagatcta atagtaaaac tggaggccgg caacatattg
     5041 tcggcctttt ttatctaaac aaatatcagt tgttctattc ataattaaaa taaaggattc
     5101 aactatgtta gattcatcaa tgagaaatca gttgtccggt atctttagcg gattgacgaa
     5161 cgaatatata tttgatatac aagtttctcc tgaacatgaa agcaggaagg aactattgga
     5221 attgctgaac gatgtggctt cctgttccga taagatcacc gtacaggtgg cagacggcga
//
