LOCUS       c00350_gnlPro..         5313 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|KCKPCBEF_350.
ACCESSION   c00350_gnlPro..
VERSION     c00350_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-05 12:42:36
            Original ID  :: gnl|Prokka|KCKPCBEF_350
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 5313
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..5313
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[677:5313]"
                     /cutoff="10000"
                     /detection_rule="(TPP_enzyme_C and not (PFL-like or
                     PFL_6_pcresol or PFL_9_tma or Gly_radical or Gly_430_pcre
                     or Gly_8-9_TMA or PFL_acetate1 or PFL_acetate2 or
                     Gly_radical_scatole or PFL_proline or PFL_toluene) and
                     minimum(2, [ALS_ss_C, AlaDh_PNT_C, Arginase, BKACE,
                     BcrAD_BadFG, Beta_elim_lyase, CPSase_L_D2, Cys_Met_Meta_PP,
                     ELFV_dehydrog, GRDB, Glu_syn_central, Glu_synthase,
                     Gly_reductase, HAAA1, HG2B1, HG2B2, HGD-D, HleuD1, HleuD2,
                     HleuS1, HleuS2, Homoserine_dh, ILVD_EDD, IlvC,
                     Lactoyl-CoA1, Lactoyl-CoA2, Lyase_aromatic, MAAL_C,
                     OKR_DC_1_5_lysine, OKR_DC_1_5_ornithine,
                     OKR_DC_1_7_arginine, OKR_DC_1_C, OTCace, PALP,
                     Proline_reductase, Sacchrp_dh_C, TPL, Thr_dehydrat_C, TnaA,
                     Urocanase]) and not (POR or POR_N or PFOR_II))"
                     /neighbourhood="10000"
                     /product="TPP_AA_metabolism"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      678..5313
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="10000"
                     /detection_rule="(TPP_enzyme_C and not (PFL-like or
                     PFL_6_pcresol or PFL_9_tma or Gly_radical or Gly_430_pcre
                     or Gly_8-9_TMA or PFL_acetate1 or PFL_acetate2 or
                     Gly_radical_scatole or PFL_proline or PFL_toluene) and
                     minimum(2, [ALS_ss_C, AlaDh_PNT_C, Arginase, BKACE,
                     BcrAD_BadFG, Beta_elim_lyase, CPSase_L_D2, Cys_Met_Meta_PP,
                     ELFV_dehydrog, GRDB, Glu_syn_central, Glu_synthase,
                     Gly_reductase, HAAA1, HG2B1, HG2B2, HGD-D, HleuD1, HleuD2,
                     HleuS1, HleuS2, Homoserine_dh, ILVD_EDD, IlvC,
                     Lactoyl-CoA1, Lactoyl-CoA2, Lyase_aromatic, MAAL_C,
                     OKR_DC_1_5_lysine, OKR_DC_1_5_ornithine,
                     OKR_DC_1_7_arginine, OKR_DC_1_C, OTCace, PALP,
                     Proline_reductase, Sacchrp_dh_C, TPL, Thr_dehydrat_C, TnaA,
                     Urocanase]) and not (POR or POR_N or PFOR_II))"
                     /neighbourhood="10000"
                     /product="TPP_AA_metabolism"
                     /protocluster_number="1"
     cand_cluster    1..5313
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(TPP_enzyme_C and not (PFL-like or
                     PFL_6_pcresol or PFL_9_tma or Gly_radical or Gly_430_pcre
                     or Gly_8-9_TMA or PFL_acetate1 or PFL_acetate2 or
                     Gly_radical_scatole or PFL_proline or PFL_toluene) and
                     minimum(2, [ALS_ss_C, AlaDh_PNT_C, Arginase, BKACE,
                     BcrAD_BadFG, Beta_elim_lyase, CPSase_L_D2, Cys_Met_Meta_PP,
                     ELFV_dehydrog, GRDB, Glu_syn_central, Glu_synthase,
                     Gly_reductase, HAAA1, HG2B1, HG2B2, HGD-D, HleuD1, HleuD2,
                     HleuS1, HleuS2, Homoserine_dh, ILVD_EDD, IlvC,
                     Lactoyl-CoA1, Lactoyl-CoA2, Lyase_aromatic, MAAL_C,
                     OKR_DC_1_5_lysine, OKR_DC_1_5_ornithine,
                     OKR_DC_1_7_arginine, OKR_DC_1_C, OTCace, PALP,
                     Proline_reductase, Sacchrp_dh_C, TPL, Thr_dehydrat_C, TnaA,
                     Urocanase]) and not (POR or POR_N or PFOR_II))"
                     /kind="single"
                     /product="TPP_AA_metabolism"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..5313
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="TPP_AA_metabolism"
                     /region_number="1"
                     /rules="(TPP_enzyme_C and not (PFL-like or PFL_6_pcresol or
                     PFL_9_tma or Gly_radical or Gly_430_pcre or Gly_8-9_TMA or
                     PFL_acetate1 or PFL_acetate2 or Gly_radical_scatole or
                     PFL_proline or PFL_toluene) and minimum(2, [ALS_ss_C,
                     AlaDh_PNT_C, Arginase, BKACE, BcrAD_BadFG, Beta_elim_lyase,
                     CPSase_L_D2, Cys_Met_Meta_PP, ELFV_dehydrog, GRDB,
                     Glu_syn_central, Glu_synthase, Gly_reductase, HAAA1, HG2B1,
                     HG2B2, HGD-D, HleuD1, HleuD2, HleuS1, HleuS2,
                     Homoserine_dh, ILVD_EDD, IlvC, Lactoyl-CoA1, Lactoyl-CoA2,
                     Lyase_aromatic, MAAL_C, OKR_DC_1_5_lysine,
                     OKR_DC_1_5_ornithine, OKR_DC_1_7_arginine, OKR_DC_1_C,
                     OTCace, PALP, Proline_reductase, Sacchrp_dh_C, TPL,
                     Thr_dehydrat_C, TnaA, Urocanase]) and not (POR or POR_N or
                     PFOR_II))"
                     /tool="gutsmash"
     CDS             2..145
                     /locus_tag="ctg350_1"
                     /transl_table=11
                     /translation="LNDLRRMNVAITRARMKLVILGDATTLTKHAFYRKLIQYIRQEAV
                     SS"
     CDS             complement(678..1721)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     TPP_AA_metabolism: IlvC"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) IlvN"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg350_2"
                     /sec_met_domain="IlvN (E-value: 2e-47, bitscore: 149.6,
                     seeds: 43, tool: rule-based-clusters)"
                     /sec_met_domain="IlvC (E-value: 1.1e-40, bitscore: 128.0,
                     seeds: 295, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAQLNFGGTVENVVIRDEFPLEKAREVLKNETIAVIGYGVQGPGQ
                     ALNLRDNGFNVIVGQRQGKTYDKAVADGWVPGETLFGIEEACEKGTIIMCLLSDAAVMS
                     VWPTIKPYLTAGKALYFSHGFAITWSDRTGVVPPADIDVIMVAPKGSGTSLRTMFLEGR
                     GLNSSYAIYQDATGNAMDRTIALGIGIGSGYLFETTFIREATSDLTGERGSLMGAIQGL
                     LLAQYEVLRENGHTPSEAFNETVEELTQSLMPLFAKNGMDWMYANCSTTAQRGALDWMG
                     PFHDAIKPVVEKLYHSVKTGNEAQISIDSNSKPDYREKLEEELKALRESEMWQTAVTVR
                     KLRPENN"
     CDS             complement(1785..2528)
                     /locus_tag="ctg350_3"
                     /transl_table=11
                     /translation="MSEENKIGTYQFVAEPFHVDFNGRLTMGVLGNHLLNCAGFHASDR
                     GFGIATLNEDNYTWVLSRLAIELDEMPYQYEKFSVQTWVENVYRLFTDRNFAVIDKDGK
                     KIGYARSVWAMINLNTRKPADLLALHGGSIVDYICDEPCPIEKPSRIKVTSNQPVATLT
                     AKYSDIDINGHVNSIRYIEHILDLFPIELYQTKRIRRFEMAYVAESYFGDELSFFCDEV
                     SENEFHVEVKKNGSEVVCRSKVIFE"
     CDS             complement(2528..3091)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     TPP_AA_metabolism: ALS_ss_C"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg350_4"
                     /sec_met_domain="ALS_ss_C (E-value: 3.3e-20, bitscore:
                     60.9, seeds: 685, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSDKTLYTIIVHSENIAGLLNQVTAVFTRRQINIESLNVSASSIK
                     GVHKYTITAWTDKDTIEKVVKQIEKKIDVIQAHYFTEDEIYFHEIALYKVSTPAFQETP
                     EASKVIRRYNARIVEVNPVFSIVEKNGMSEEITSLYEELRALKCVLQFVRSGRVAITTS
                     CFERVNEFLDGQEAKYKQSKKEQE"
     CDS             complement(3110..4807)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     TPP_AA_metabolism: TPP_enzyme_C"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg350_5"
                     /sec_met_domain="TPP_enzyme_C (E-value: 5.1e-47, bitscore:
                     148.2, seeds: 323, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSKDLITGAEAMMRSLEHQGVTTIFGYPGGSIMPTFDALYDHQNT
                     LNHILVRHEQGAAHAAQGYARVSGKVGVCLVTSGPGATNTITGIADAMIDSTPIVVIAG
                     QVGTGFLGTDAFQEVDLVGITQPIAKWSYQIRRAEDVAWAIARAFYIASSGRPGPVVLD
                     FAKNAQVEKTKYEPTKQEFIRSYVPVPDTDEESVKAAAELINNAERPLVLVGQGVELGS
                     AQEELRIFIEKADMPAGCTLLGLSALPTDHPLNKGMLGMHGNLGPNINTNKCDVLIAVG
                     MRFDDRVTGNLATYAKQAKVIHFDIDPAEVNKNVKVDIAVLGDCKKTLAAVTGLLKKNR
                     HTEWVDSFKEYEAVEEEKVIRPELHPATDSLSMGEVVRAVSEATRHEAILVTDVGQNQM
                     ISARYFKYTRERSIVTSGGLGTMGFGLPAAIGATFGRPDRIVCVFMGDGGLQMNIQELG
                     TIMEQKAPVKIICLNNNYLGNVRQWQAMFFNRRYSFTPMLNPDYMKIASAYDIPSKRVF
                     SREELKAAIDEMLSTDGAFLLEACVVEEGNVLPMTPPGGSVNQMLLEC"
     CDS             complement(4855..5313)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     TPP_AA_metabolism: ILVD_EDD"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg350_6"
                     /sec_met_domain="ILVD_EDD (E-value: 2.1e-61, bitscore:
                     196.7, seeds: 918, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="PAKVFDSQEAACEGILGGRVVSGDVVVITHEGPKGGPGMQEMLYP
                     TSYIKSRHLGKECALITDGRFSGGTSGLSIGHVSPEAAAGGNIGKIVDGDIIEIDIPAR
                     TINVRLTDEELAARPMTPVTRDRYVPKSLKAYASMVSSADKGAVRLID"
ORIGIN
        1 tctgaatgat ttaagacgaa tgaacgtagc cattacccgc gcccggatga aactcgtgat
       61 tttgggagat gcaaccactc ttaccaagca tgcgttctac cggaaactga tacagtatat
      121 ccggcaggaa gcggtatctt cctgaatcat cggtataaaa aaagaagccc cctttctcac
      181 gaaagaggac tcccatttac aaacaaaaca aacaatgtta ttttcccact aaattctgtt
      241 ccaaaagctc tgtcttaaat tcaatttaac gcatactttt tcctgctaca gaataaaatt
      301 ctgcatcata tcaacgaatc actaatggaa cacgaaatat aaaaaggact tgaaatggta
      361 ttacagctac cattcggtag agaataactt aaatatcttt taatgtaaca ttctgatata
      421 ctatttatta aagaagaaat catcttcata cgtatcgatc ttctgtaagt aaaaagtttc
      481 ttcattgcct attctgtttt atttgttgcc gtaaaggtaa tcataaatgc tctttttcac
      541 ctatattatc agtatctttt aatctttttt tctactttca tcctttttct attactcaaa
      601 agaccggtat ctacatagaa aaggcgggaa aatacgcttt tcccgccttt tgaaaaactt
      661 atatagtata agtctgatta attattttcc ggacgaagtt tacgaactgt cacggcagtc
      721 tgccacattt cgctttcgcg caatgctttc agttcttctt ccagtttctc acgataatcc
      781 ggtttggagt tagagtcgat tgaaatctgt gcttcgttac cagtcttcac actgtgatac
      841 aacttttcaa ctaccggttt gatagcatcg tggaaggggc ccatccagtc gagagcacca
      901 cgttgagctg tagtagagca gttagcatac atccagtcca taccgttctt tgcaaacaac
      961 ggcatcaatg actgagtcag ctcttctaca gtttcgttga atgcttcgga aggagtgtga
     1021 ccgttttcac gcaacacttc gtattgtgcc agcaacagac cctggatagc tcccatcaat
     1081 gaaccacgtt cgcctgtcag gtcggaagta gcttcgcgga tgaaagttgt ttcgaacaaa
     1141 taacctgaac cgataccgat acccaatgcg attgttctgt ccatagcgtt gcctgttgca
     1201 tcctgataga tagcgtatga agagttcaac ccgcgacctt caaggaacat agtacgcaag
     1261 gatgtacccg aacctttagg agcaaccatg attacgtcga tatctgcagg aggaactaca
     1321 cctgtgcggt cactccatgt aatagcaaaa ccatgagaga aataaagagc ttttcctgca
     1381 gtcaggtaag gcttgatagt aggccataca gacattactg ctgcatcaga caacaggcac
     1441 ataatgatcg tacctttttc gcaagcttct tcaataccga acaaagtttc acccggaacc
     1501 catccgtcag caaccgcttt gtcatatgtc tttccctggc gttgaccaac gattacattg
     1561 aaaccgttat cacgaaggtt cagagcctgt ccgggacctt gtacgccata accgattaca
     1621 gcgattgttt catttttcaa tacttcacga gctttttcca atggaaattc atcacggata
     1681 actacatttt ctacagttcc gccaaaattc aactgtgcca ttttttttgt ttttttgttt
     1741 tgatgacttg cgccatctgt tattaattga ttttatactc tatattattc aaatatcact
     1801 ttggaacggc atactacctc gctgccgttt ttcttcacct ctacatgaaa ctcgttttca
     1861 ctcacctcat cacagaagaa agagagttca tctccgaaat aactttcagc cacatacgcc
     1921 atttcaaacc ggcggatacg tttcgtctga tacagttcta tcggaaacaa gtcaagtatg
     1981 tgctcgatat aacgaatact gtttacgtgt ccgttaatat cgatatcact gtattttgcc
     2041 gtcagcgtcg caacaggctg attgcttgtc accttgatac gcgaaggctt ttctatcggg
     2101 cagggttcat cgcatatata atcgacgatg cttcctccat gcaatgccaa cagatcggca
     2161 ggcttacggg tattcagatt gatcatcgcc catacggaac gggcatagcc gatcttcttt
     2221 ccatccttgt caatgacggc aaagttacgg tcggtaaaga gacgatagac attctccacc
     2281 catgtctgaa ccgagaactt ctcgtactgg taaggcatct catccagctc gatagccagg
     2341 cgggaaagca cccatgtgta attgtcctcg ttcagcgtag caataccaaa tccacggtcg
     2401 ctggcatgaa aacctgcgca attcaacaga tgatttccca gcacgcccat ggtcaggcgg
     2461 ccgttaaaat ctacgtgaaa cggctctgcc acaaactgat aagtccctat tttattttct
     2521 tcactcatta ctcctgttcc tttttacttt gcttatactt cgcttcctgt ccgtccagga
     2581 actcattcac acgttcgaaa cagctggtag taatagccac acgacccgaa cggacaaatt
     2641 gcagcacaca tttcaaagcc cgaagttctt cgtaaaggga agtaatctct tcactcatgc
     2701 cgttcttctc gacgatagag aataccggat tcacctctac aatgcgggca ttatatctgc
     2761 ggatcacttt agatgcttcc ggagtctcct gaaaagcagg agtagatacc ttatataagg
     2821 ctatctcatg gaaataaatc tcgtcttcag taaaatagtg cgcctgaatc acatcaatct
     2881 tcttttcgat ctgcttcacc accttttcaa tagtgtcctt atcagtccac gctgtgatcg
     2941 tatatttatg tactccttta atggaagaag cagaaacgtt caaactctcg atgtttattt
     3001 gccgacgggt aaatacagcg gtcacctgat tcagcaatcc ggctatattt tcagaatgaa
     3061 ctataatcgt atataatgtc ttatcactca taactttcta aattaaggat taacattcca
     3121 gcaacatctg attgactgaa ccgcccggag gggtcatcgg gaggacattg ccttcttcta
     3181 ccacacaggc ttcgagcagg aacgccccgt ctgtcgagag catttcgtcg atggccgctt
     3241 tcaattcttc ccgtgaaaag acacgtttgg aaggaatatc gtaggcggaa gcgattttca
     3301 tgtaatccgg attcagcatc ggagtaaatg aataacggcg attaaagaac atagcctgcc
     3361 attggcgcac gttgccgaga taattgttgt tcaggcagat aatcttcacc ggagctttct
     3421 gttccatgat cgtacccagt tcctgaatgt tcatttgcag tccgccgtca cccataaata
     3481 cacatacaat gcggtcagga cggccgaaag tagctccgat agctgccgga aggccaaaac
     3541 ccatcgtgcc gagtccaccc gaagtaacga tactacgttc cctggtatat ttaaagtagc
     3601 gggcagagat catctgattc tgacctacat ctgtcaccaa tatggcttcg tgacgggtcg
     3661 cttcgcttac cgcacgcacc acttcgccca tactcagtga atccgttgca ggatgaagtt
     3721 caggacgaat gactttttct tcctctaccg cttcatattc cttgaaacta tcgacccact
     3781 cggtgtgccg gttctttttc aacagtccgg tcacagcagc cagcgtcttt ttgcagtcgc
     3841 ccaaaacggc aatatctacc tttacattct tatttacttc cgccggatca atgtcaaaat
     3901 ggatcacttt cgcttgtttg gcgtaagtag ccagattacc ggtcacacgg tcgtcgaaac
     3961 gcataccgac agcaatcagt acatcacatt tgttagtatt gatattcggt cccagatttc
     4021 cgtgcatacc cagcattcct ttattcaacg gatgatcggt aggcaatgcg gaaagtccga
     4081 gcaaggtaca acctgcgggc atatccgcct tttcgataaa gatacgcagt tcttcctgag
     4141 cgcttcccag ttccactccc tgtcctacca agacaagcgg gcgttcggcg ttattgatca
     4201 gttcggcggc agcctttacc gattcttcgt ctgtatcggg aaccggaaca tagctgcgga
     4261 taaactcctg ctttgtcggc tcgtatttcg ttttttcaac ctgcgcattc ttggcaaagt
     4321 cgagtaccac cggaccggga cgaccactac tggcaatgta gaaagcacgt gctatcgccc
     4381 aggctacgtc ttcggcacgg cgaatctgat agctccattt agcgattggc tgagtgatac
     4441 ctacgagatc gacttcctga aaagcgtctg ttccaagaaa gcctgttccc acctgtcctg
     4501 caatgacaac gataggcgta ctgtctatca ttgcatctgc aatacctgtg atcgtattgg
     4561 ttgctccggg gccgcttgtc accaagcaaa caccgacttt acccgataca cgggcgtagc
     4621 cttgtgccgc atgggcagct ccctgttcat ggcgaaccaa gatatggttc aatgtatttt
     4681 gatggtcgta cagggcatcg aacgtaggca tgatggaccc gccgggataa ccgaaaatgg
     4741 tagtcactcc ctgatgttcc agagagcgca tcattgcctc tgcgcctgtt attaagtcct
     4801 tactcatagt tctttaatgg ataatggatg attcataatg gataatgaag tggatcagtc
     4861 tattaatctc actgctccct tatcggcaga gcttaccatg ctggcgtatg ctttcaggct
     4921 cttcggcacg taacggtcac gggtcacggg agtcatcggg cgggcagcca gttcttcatc
     4981 tgttagtcgc acgttaatcg ttcgggcggg aatgtcgatt tcgatgatat ctccatctac
     5041 tatttttcct atattacctc cggcggcggc ttcgggagaa acgtgtccga tactcaatcc
     5101 ggaagttcca ccactgaaac gtccatcggt aatcaaggca cattctttgc cgaggtgacg
     5161 ggatttgata taagaagtcg ggtaaagcat ttcctgcata ccgggaccgc ccttcggacc
     5221 ttcgtgcgtg atgacgacaa cgtcgccgct gacgactcgg cctccgagga tgccttcaca
     5281 ggctgcttct tgtgagtcga atactttggc agg
//
