LOCUS       c00269_gnlPro..         7538 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|NABGPNMH_269.
ACCESSION   c00269_gnlPro..
VERSION     c00269_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-07 20:31:42
            Original ID  :: gnl|Prokka|NABGPNMH_269
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 7538
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..7538
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[5161:7537]"
                     /cutoff="10000"
                     /detection_rule="(Succ_DH_flav_C and FAD_binding_2 and
                     (Fer2_3 or Fer4_7 or SucD_FumR))"
                     /neighbourhood="10000"
                     /product="Fumarate2succinate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      5162..7537
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="10000"
                     /detection_rule="(Succ_DH_flav_C and FAD_binding_2 and
                     (Fer2_3 or Fer4_7 or SucD_FumR))"
                     /neighbourhood="10000"
                     /product="Fumarate2succinate"
                     /protocluster_number="1"
     cand_cluster    1..7538
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(Succ_DH_flav_C and FAD_binding_2 and
                     (Fer2_3 or Fer4_7 or SucD_FumR))"
                     /kind="single"
                     /product="Fumarate2succinate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..7538
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Fumarate2succinate"
                     /region_number="1"
                     /rules="(Succ_DH_flav_C and FAD_binding_2 and (Fer2_3 or
                     Fer4_7 or SucD_FumR))"
                     /tool="gutsmash"
     CDS             33..224
                     /locus_tag="ctg269_1"
                     /transl_table=11
                     /translation="MKKPQYKDKPETSVCQVFYRQYMLSLSGIVAKNCFRTQNFIQYRQ
                     KLLILTVKTAKSIEKDVL"
     CDS             complement(202..315)
                     /locus_tag="ctg269_2"
                     /transl_table=11
                     /translation="MRVELFGKESYQVRLKTGVCLRASLTGYKLLKERLSL"
     CDS             complement(329..643)
                     /locus_tag="ctg269_3"
                     /transl_table=11
                     /translation="MQTHPFINSVLCRMAGVALAVVSCSISALFLYYYGHTGWLVAWLD
                     SLVSVGIFCVSGFLFWYVTGILRPLQAQIALAVGVLIISVTGGYMAGALVYPYSPFLYR
                     "
     CDS             complement(646..1395)
                     /locus_tag="ctg269_4"
                     /transl_table=11
                     /translation="MNLRKNLFWAIPFIVVGVVWIMRNTGMITDDLFHIIVSWQMLVMY
                     IGIGSISRRHYIGGLITFLVGAAFLLPRLGWMDSDWLQVYWPAAFVVVGLMLIFEPRKK
                     NSYHYNSRREDTGGEDIRGNAVAGKVKSDFINTDGYVESDNTFGYVEQIILDPVFKGAR
                     IRTVFGGTVLDLRKTKLDSAKTYIDIDCKFAGVEIYLPGDWAVNFHGSPIMGACEDKRF
                     NASMEMDKEHILVVRGIVMFGGIEFKS"
     CDS             complement(1801..2385)
                     /locus_tag="ctg269_5"
                     /transl_table=11
                     /translation="MTTKLKDLFDTFLNIAASSQKLGINLIRVAILIIFVWIGGLKFYN
                     YEAEGIVPFVANSPFMSFFYAKSAPEYKEYKLKEGEFNEAKHQWHVENNTYIFSHGLGI
                     MIMAIGILTFLGIFSPKIGLIGSGLAIIMTLGTLSFLVTTPEVWVPDLGSGEHGFPLLT
                     GAGRLVIKDTAIIAGAIVVLSDCAQRILKKK"
     CDS             complement(2563..3777)
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Radical_SAM"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg269_6"
                     /sec_met_domain="Radical_SAM (E-value: 1.3e-14, bitscore:
                     44.2, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSTKRTKSSFRTMSTNRKLESVFLFVTGRCNAKCKMCFYAEDMDK
                     KEKDLTFEEIKTISETAGQFNRLWVSGGEPTLREELPEIIEMFYKNNEIKDVNIPTNGL
                     KPDRIVAWVKQIRQNCPELNINLSLSLDGFGDTHDIQRGVPGNFYKAMGTLKLIEKNFA
                     DDGRVLKNLATVITKYNENEVEDLMIWMLGRFHLSNHIIEAARGMTRDDGVKVLTEPTL
                     RALQDKVVPIYNVYGERMAEETGGFRKPIARFFYLGFIRTLYNVRATNIDHPTPWGMDC
                     TAGETTLVIDYDGRFRACELREPLGNVKDYDCNIAKIMESDAMKQEIAAIGHGDKANCW
                     CTHGCWITSSLVFNPRKMITQVYKGYREMKKLNRPLNISEELLQSIERKYALDEQKLVE
                     LGVRN"
     CDS             4018..4863
                     /locus_tag="ctg269_7"
                     /transl_table=11
                     /translation="MEDNIPKFDLPVDFIVGNSITGEILNQYGRFPCKIKAGIFVLCVQ
                     GTVHATVNLTEFTIRKNDFITLPPGCFIQIHEVSDDVKLCFAGFSSVFVSHINYIKTIT
                     NYLPVIFDSPIMPLPEHISLLYQQAFDLLINAYSMPKTIENKEIIKAIFTIFMQGVIEL
                     YRNHTPYSNAPMTRNMEICREFVQLAMENYTKEHSVSYYAKRLNITLQHFCYVIKKVSG
                     RTALDILSNIIIMDAKAQLKSTDMPVKKIAFALGFDNLSFFNKYFRQHVGMTPQEYRES
                     "
     CDS             complement(5162..5917)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Fumarate2succinate: Fer2_3"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Fer4_8"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Fer4_4"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg269_8"
                     /sec_met_domain="Fer2_3 (E-value: 6.1e-27, bitscore: 83.2,
                     seeds: 38, tool: rule-based-clusters)"
                     /sec_met_domain="Fer4_8 (E-value: 2e-12, bitscore: 36.8,
                     seeds: 153, tool: rule-based-clusters)"
                     /sec_met_domain="Fer4_4 (E-value: 2.1e-06, bitscore: 17.5,
                     seeds: 79, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDKDIKVTLKVWRQKSPKDKGQFETYQIDKINQSVSFLEMLDILN
                     ERLVSEGKEPIVFDHDCREGICGMCSLYVNGHPHGPATGATTCQLYMRRFNDGETITIE
                     PWRSAGFPVIRDLMVDRYAYDKIIQAGGFVSVNTGGVPDANSIAIPRADAEMAMDAAAC
                     IGCGACAAACKNGSAMLFVSAKVSQLALLPQGKVEAARRAKAMVAKMDELGFGNCTNTR
                     ACEMECPKNISVSNIARLNREFICAKLQD"
     CDS             complement(5945..7537)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Fumarate2succinate: FAD_binding_2"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Fumarate2succinate: Succ_DH_flav_C"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1027:FAD binding domain (Score: 131.3; E-value:
                     1.7e-40)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg269_9"
                     /sec_met_domain="FAD_binding_2 (E-value: 2.4e-41, bitscore:
                     131.3, seeds: 62, tool: rule-based-clusters)"
                     /sec_met_domain="Succ_DH_flav_C (E-value: 4.8e-39,
                     bitscore: 122.6, seeds: 739, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="AEVSNSIIDQCVAQGVPFAREYGGLLDNRSFGGAQVSRTFYARGQ
                     TGQQLLLGAYSALSRMIGLNKVKMYTRHEMLDVVKVDGRARGIIARNLITGQIERFSAH
                     AVVVATGGYVNTFFLSTNAMASNGSAAWQCYKKGAYFANPCMVQIHPTCVPVKGDYQSK
                     LTLMSESLRNDGRIWVPKKLEDAKALQAGTKKGKDIPEADRDYYLERRYPAFGNLVPRD
                     VASRAAKERCDAGFGVNNTGLAVFLDFSDAINRLGKDVVKAKYGNLFDMYEEITNDDPY
                     ETPMMIYPALHYSMGGLWVDYELMTSIPGLFAIGEANFSDHGANRLGASALMQGLADGY
                     FVLPYTIQNYLSDQIQVPRFSTDLPEFAEAEKGIKDRIKKLMSIKGKESVDSIHKKLGH
                     IMWEHVGMARDAKGLQEAIQMLKELKKEFWSNVYIPGEADNLNVELDKALRLADFIEIG
                     TLMAHDALDRAESCGGHFRTEHQTEEGEALRHDDKFMYVSCWEYQGEDKDPVMLKEDLN
                     YQFVVPQTRNYKK"
ORIGIN
        1 ccaactttgt taaccttaaa tctaatacta ttatgaaaaa accacaatac aaagataagc
       61 ctgaaacaag cgtttgtcaa gtcttttaca gacaatatat gttaagcctt tcgggcattg
      121 tagctaaaaa ttgtttccga acacaaaact ttatacaata cagacaaaaa ctgctaattt
      181 taacagttaa aacagccaaa tctatagaga aagacgttct ttaagcaact tgtatccggt
      241 caggctggca cgcagacaga cacctgtttt aagacgaacc tggtaacttt ccttgccgaa
      301 cagttccaca cgcatgatct gttcgctatt aacgatacag gaacggtgaa tacggataaa
      361 ccagcgcccc ggccatatat cctccggtaa cggatatgat aaggacgcca acagccaatg
      421 caatctgggc ctgtaatggc cgcaatatgc ctgttacata ccagaacagg aaaccggaga
      481 cacagaatat accgaccgaa accagactat ccaaccatgc gacaagccat cccgtatgtc
      541 cgtaataata gaggaacaag gcgcttatgc tgcaactgac cacagccaaa gccacaccag
      601 ccattctaca caggacagaa tttataaacg gatgtgtctg cataatcaac tcttaaattc
      661 aatgccaccg aacataacaa ttccccgtac aaccagtata tgttctttat ccatttccat
      721 agaggcatta aaacgtttat cctcgcatgc ccccatgatc ggactgccat gaaaattgac
      781 cgcccaatcc ccaggaagat agatttccac accggcaaac ttacaatcga tatcaatata
      841 ggttttagca ctatccagct tcgttttccg caaatccagg accgttcccc caaacacagt
      901 cctaatcctg gctcctttaa ataccggatc taaaataatt tgttccacat agccaaatgt
      961 attatccgat tccacatatc cgtccgtatt aataaaatca ctttttactt taccggcaac
     1021 ggcatttccc cggatatctt ctccaccggt atcctctctc cggctattat aatgatagga
     1081 gttcttttta cgcggttcga agatcagcat taaaccgaca acgacaaaag cagccggcca
     1141 atagacctgc aaccaatcgc tatccatcca ccccagtcgt ggcagtaaaa atgccgcacc
     1201 aaccaaaaag gtaatcaaac cgcctatata atgtctccgg gaaatactcc ctatacctat
     1261 atacattaca agcatctgcc aggatacaat gatatgaaaa agatcatccg tgatcatgcc
     1321 tgtgttcctc atgatccata cgacccccac tacaataaac gggatggccc aaaataagtt
     1381 tttcctaaga ttcatacgat atacagttta attgtttgag agcaaaagta tgctcctgtc
     1441 agccttccgg caatagaaat acgtcctatt acagtcaaat atcggtgaat tgccgaatat
     1501 taccgataaa aaagggagtt atttttcaga gaatgccata tttaaaagaa aaaaggcttg
     1561 catttccgaa tgcaaacccc tataaacaat taaagcctgt ctcacgacaa gccctaacca
     1621 actttgttaa ccttaaatct aatactatta tgaaaaaacc acgatgtaaa tatactgctt
     1681 aatccaaacc tgtcaagagg ttttatgcta aatggtgttt catacactat aagaaaagct
     1741 aacaaggttc aatatgtttg ttttttgctt ccaaagtgtt aaaataaaca ctttttgttc
     1801 ctatttcttc tttaatatac gttgtgcaca gtcggacaat accacgatcg ctccggctat
     1861 gattgccgta tcttttatta ccagccgacc tgctccggtc agtaagggga aaccatgttc
     1921 tccgcttccc aaatcgggca cccatacttc aggtgttgtc accaggaatg aaagcgtacc
     1981 caatgtcatt atgatcgcca gtccggaacc tatcaagcca attttcggag aaaagatacc
     2041 caggaaagtc aggataccga tcgccatgat cataatgcct agtccatgtg aaaaaatata
     2101 ggtattgttt tctacatgcc actgatgctt ggcttcattg aattcacctt ctttcaactt
     2161 atattctttg tattccggtg cactctttgc atagaaaaag ctcataaacg gactgtttgc
     2221 cacaaaagga acgatccctt ccgcttcata attatagaat ttcagcccgc cgatccatac
     2281 gaatataatc aggatagcta cgcggatcag gttaataccc agtttctgtg aagaagctgc
     2341 aatgttcaga aatgtgtcga ataaatcttt taatttagta gtcatgtctt tctttgttta
     2401 tcagtttaac ttatgatgca aagatccgac atttttcccc tatgggaaat ggcaattcta
     2461 ccagataatt tgacaattat tcccttaaac acaataaagc cggttaacag aggtatattt
     2521 cccgtcaacc ggctttataa atgatccgtt atcggaacta ttttaattac gaacccccag
     2581 ctccaccagt ttctgttcgt ccaaggcata tttgcgttcg atgctctgca aaagctcttc
     2641 actgatattc agaggacggt tcagcttctt catctcccgg tatcctttat agacctgcgt
     2701 gatcatcttg cggggattga aaacaagcga tgaagtgatc caacacccat gcgtacacca
     2761 gcagttagcc ttgtcaccat gaccgatagc ggcaatctcc tgtttcatgg catcgctctc
     2821 cataatcttt gcgatattgc aatcataatc ctttacatta ccgagcggtt cgcgaagttc
     2881 gcaggcacgg aaacgaccgt catagtctat taccagtgtt gtttcgccgg ccgtacaatc
     2941 cataccccag ggggtcggat gatcgatatt ggtcgcacgt acattatata atgtacgtat
     3001 gaaacccagg tagaagaaac gggcgatagg ttttctgaac ccaccggttt cctccgccat
     3061 acgctctcca tatacattat aaataggaac aaccttatcc tgcaaggcgc gtaaagtagg
     3121 ctcggtaaga accttcactc cgtcgtcgcg ggtcatgccg cgtgcagctt cgataatatg
     3181 attggaaaga tggaaacgtc ccagcatcca tatcatcaga tcctcgacct cattctcgtt
     3241 atatttggtg attacggtgg ccaggttttt caacaccctg ccgtcgtcag caaagttttt
     3301 ttcaatcagc ttcagtgtcc ccatcgcttt gtagaaattg ccgggaacgc cgcgctgtat
     3361 atcatgcgta tcaccgaaac cgtccaaaga aaggctcagg ttgatattca gttccggaca
     3421 attctggcgg atctgtttta cccaggcgac gatccggtca ggcttcaatc cgttggtagg
     3481 gatattcacg tcctttatct cgttattctt gtagaacatc tctataattt caggtaactc
     3541 ctcacgcagt gtcggttcac cgccggatac ccacaaacgg ttgaactgcc cggctgtttc
     3601 ggagattgtc ttgatttctt caaaagtcag gtctttctcc tttttatcca tgtcttctgc
     3661 atagaaacac attttacatt tagcattaca ccgtcctgtg acaaacagga aaacagattc
     3721 taattttctg tttgtgctca ttgtcctgaa agagctcttt gttcttttcg tactcattgt
     3781 tattatcgat tagatttctc tattagtatt agctcccata tcttatttag cagatagaca
     3841 aatatatacc atcccaacaa atctatcagc aagacttgaa aacattcgca aatttcaact
     3901 attatacagc acaaaacaat ctccatttgt gggattcgat gttctatatt taagattatc
     3961 gactaacttg tctcaaaaaa actatctttg tacattcaat taagtgtata taaaagtatg
     4021 gaagacaata ttcctaaatt cgacctcccc gtagatttta ttgtaggaaa cagtattaca
     4081 ggtgaaatac tgaaccaata cggacgtttc ccgtgcaaga tcaaggcagg catattcgtg
     4141 ctttgcgtac aagggacagt ccatgcgact gtaaacttaa ctgagtttac catccggaaa
     4201 aacgacttta tcaccttgcc cccgggttgc ttcattcaga tacatgaggt ttccgatgat
     4261 gtaaaactgt gttttgcagg cttttcttcc gtattcgtca gccatatcaa ttatataaag
     4321 acgattacga actatctgcc tgtcatattc gacagtccga tcatgccttt gccggagcat
     4381 atatccttac tctaccaaca ggctttcgac ttgctgatca atgcttattc catgcctaaa
     4441 accatcgaaa acaaggagat tatcaaggct atctttacta tcttcatgca aggggttatt
     4501 gaattataca ggaatcatac gccatatagc aatgctccca tgacgcgtaa tatggaaata
     4561 tgccgggagt tcgtacagct ggcgatggaa aactacacga aagagcatag cgtgtcttat
     4621 tacgccaaaa ggctcaacat caccttgcag catttctgtt atgtgataaa aaaggtcagc
     4681 ggacggactg cattggatat cctgagcaat atcatcatta tggatgccaa agcgcagctt
     4741 aaatcgaccg atatgccggt caagaaaatt gcattcgccc tgggcttcga caacctttcg
     4801 ttcttcaaca aatatttccg gcaacatgta ggaatgactc cgcaggaata cagggagagc
     4861 taaaaaaagg gatatgccgg acgcaaaccc ctataaacaa ttaaagcctg tctcacgaca
     4921 agccctaacc aactttgtta accttaaatc taatactatt atgaaaaaac catagtacaa
     4981 agatacaggt attctccaac ttgtcaatag ttaaaatgct aattagtgtt gtatagcata
     5041 taagaaacac taacaacatt caagatattt atctatgact tcagaaatgt taaaataggg
     5101 gcaaaaaaga agaggcgccg ccataagcaa cgcctctccg gacatacata tataatgtat
     5161 attagtcttg caatttagca caaatgaact cgcggttcaa acgagcgatg ttgctgacag
     5221 agatgttctt cggacattcc atttcgcaag cacgcgtgtt ggtacagtta ccgaagccca
     5281 gttcgtccat cttggcaacc attgctttcg cacgacgagc agcttctacc ttaccctgag
     5341 gcaacagagc caactggcta acctttgcag aaacgaacaa catagccgaa ccgttcttac
     5401 aagcagcagc acaagcaccg caaccgatac atgcagcagc atccatagcc atttcagcat
     5461 ctgcacgagg aatagcgatt gaattggcat caggtacacc acctgtgttc actgatacga
     5521 aaccacctgc ctggataatt ttatcgtaag cataacggtc taccatcaag tcgcggatta
     5581 ccgggaaacc ggcagaacgc cacggttcga ttgtaatagt ttcgccatca ttgaagcgac
     5641 gcatataaag ctggcaagta gttgcgccgg ttgcaggtcc gtgcggatgt ccgtttacat
     5701 acaaagaaca cataccgcag ataccttcac gacagtcgtg atcgaatacg atcggctctt
     5761 taccttcact gaccaaacgt tcattcaaaa tgtctaacat ttccaggaaa gaaacactct
     5821 gattgatttt gtctatttgg taagtctcaa attgtccttt atctttcgga cttttctgac
     5881 gccaaacttt aagtgttact tttatatctt tatccatgat tctaattttt gtgattgatg
     5941 attattactt cttgtagtta cgtgtttgag gaacaacgaa ctgatagttc aaatcttcct
     6001 tcagcataac cggatctttg tcttcaccct ggtattccca acaagatacg tacatgaact
     6061 tatcgtcatg acgcagggct tcgccttcct cggtctggtg ctctgtacgg aagtgtcctc
     6121 cacaagattc ggcacggtcc aatgcatcgt gtgccatcaa tgtaccaatc tcaataaagt
     6181 cagccaaacg caatgcctta tccaactcta cgttcaggtt atcggcttca cccggaatat
     6241 atacattact ccagaactct ttcttcagct ctttcaacat ctggatcgct tcctgcaaac
     6301 cttttgcatc acgtgccata cctacatgtt cccacatgat atgacccagt ttcttatgga
     6361 tagaatctac cgactcttta cccttgatac tcatcaattt cttgatgcgg tctttgatcc
     6421 ccttttcagc ttctgcaaac tcaggcaggt cggtactgaa acgcggaacc tggatctggt
     6481 ctgacagata gttctggatt gtataaggca atacaaagta gccatcggcc aatccctgca
     6541 tcaacgcaga agcacccaga cggttagctc cgtggtcaga gaagttagct tcaccgatag
     6601 cgaacagacc cggaatagaa gtcatcaatt catagtcaac ccacagacca cccatcgagt
     6661 agtgcaatgc aggataaatc atcatcggag tttcgtatgg atcgtcgttg gtaatttctt
     6721 cgtacatgtc gaacaagtta ccatatttag cttttacaac atctttaccc agacggttga
     6781 tggcgtcgga gaagtcgagg aacacggcca gacctgtgtt gttcacaccg aagccggcgt
     6841 cgcaacgttc tttagcggca cgtgaagcca cgtcacgcgg aaccaggtta ccgaatgccg
     6901 gataacggcg ttccagatag tagtcgcggt ctgcttcagg aatgtctttc cctttcttag
     6961 ttccggcctg taatgctttt gcatcttcca gtttcttcgg aacccagata cgaccgtcgt
     7021 tacgcaatga ttcagacatc aaagtcagct tagactggta gtcacctttc accggtacac
     7081 aagtcgggtg gatctgcacc atacaagggt tagcgaagta agcacctttc ttgtagcact
     7141 gccatgcagc agaaccgttg gaagccattg cgttagtaga caggaagaaa gtatttacat
     7201 aaccacctgt tgccaccact actgcgtgag cagagaaacg ttcgatctga cctgtgatca
     7261 ggttacgtgc gatgataccg cgggcacggc catccacctt cacaacgtcc agcatttcgt
     7321 ggcgggtata catctttact ttgttcaaac cgatcatacg gctcaaagca gagtaagcac
     7381 ccagcaacaa ctgctgtccg gtctgaccgc gggcatagaa cgtacgggat acctgagcac
     7441 caccgaatga acggttatcc agcagaccac cgtattcgcg agcgaaagga acaccctgag
     7501 ctacgcactg gtcgataata gagttggaaa cttcagcc
//
