LOCUS       c00232_gnlPro..         4657 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|GOLEIIAB_232.
ACCESSION   c00232_gnlPro..
VERSION     c00232_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-07 20:52:18
            Original ID  :: gnl|Prokka|GOLEIIAB_232
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 4657
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..4657
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[233:3887]"
                     /cutoff="10000"
                     /detection_rule="(TPP_enzyme_C and not (PFL-like or
                     PFL_6_pcresol or PFL_9_tma or Gly_radical or Gly_430_pcre
                     or Gly_8-9_TMA or PFL_acetate1 or PFL_acetate2 or
                     Gly_radical_scatole or PFL_proline or PFL_toluene) and
                     minimum(2, [ALS_ss_C, AlaDh_PNT_C, Arginase, BKACE,
                     BcrAD_BadFG, Beta_elim_lyase, CPSase_L_D2, Cys_Met_Meta_PP,
                     ELFV_dehydrog, GRDB, Glu_syn_central, Glu_synthase,
                     Gly_reductase, HAAA1, HG2B1, HG2B2, HGD-D, HleuD1, HleuD2,
                     HleuS1, HleuS2, Homoserine_dh, ILVD_EDD, IlvC,
                     Lactoyl-CoA1, Lactoyl-CoA2, Lyase_aromatic, MAAL_C,
                     OKR_DC_1_5_lysine, OKR_DC_1_5_ornithine,
                     OKR_DC_1_7_arginine, OKR_DC_1_C, OTCace, PALP,
                     Proline_reductase, Sacchrp_dh_C, TPL, Thr_dehydrat_C, TnaA,
                     Urocanase]) and not (POR or POR_N or PFOR_II))"
                     /neighbourhood="10000"
                     /product="TPP_AA_metabolism"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      234..3887
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="10000"
                     /detection_rule="(TPP_enzyme_C and not (PFL-like or
                     PFL_6_pcresol or PFL_9_tma or Gly_radical or Gly_430_pcre
                     or Gly_8-9_TMA or PFL_acetate1 or PFL_acetate2 or
                     Gly_radical_scatole or PFL_proline or PFL_toluene) and
                     minimum(2, [ALS_ss_C, AlaDh_PNT_C, Arginase, BKACE,
                     BcrAD_BadFG, Beta_elim_lyase, CPSase_L_D2, Cys_Met_Meta_PP,
                     ELFV_dehydrog, GRDB, Glu_syn_central, Glu_synthase,
                     Gly_reductase, HAAA1, HG2B1, HG2B2, HGD-D, HleuD1, HleuD2,
                     HleuS1, HleuS2, Homoserine_dh, ILVD_EDD, IlvC,
                     Lactoyl-CoA1, Lactoyl-CoA2, Lyase_aromatic, MAAL_C,
                     OKR_DC_1_5_lysine, OKR_DC_1_5_ornithine,
                     OKR_DC_1_7_arginine, OKR_DC_1_C, OTCace, PALP,
                     Proline_reductase, Sacchrp_dh_C, TPL, Thr_dehydrat_C, TnaA,
                     Urocanase]) and not (POR or POR_N or PFOR_II))"
                     /neighbourhood="10000"
                     /product="TPP_AA_metabolism"
                     /protocluster_number="1"
     cand_cluster    1..4657
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(TPP_enzyme_C and not (PFL-like or
                     PFL_6_pcresol or PFL_9_tma or Gly_radical or Gly_430_pcre
                     or Gly_8-9_TMA or PFL_acetate1 or PFL_acetate2 or
                     Gly_radical_scatole or PFL_proline or PFL_toluene) and
                     minimum(2, [ALS_ss_C, AlaDh_PNT_C, Arginase, BKACE,
                     BcrAD_BadFG, Beta_elim_lyase, CPSase_L_D2, Cys_Met_Meta_PP,
                     ELFV_dehydrog, GRDB, Glu_syn_central, Glu_synthase,
                     Gly_reductase, HAAA1, HG2B1, HG2B2, HGD-D, HleuD1, HleuD2,
                     HleuS1, HleuS2, Homoserine_dh, ILVD_EDD, IlvC,
                     Lactoyl-CoA1, Lactoyl-CoA2, Lyase_aromatic, MAAL_C,
                     OKR_DC_1_5_lysine, OKR_DC_1_5_ornithine,
                     OKR_DC_1_7_arginine, OKR_DC_1_C, OTCace, PALP,
                     Proline_reductase, Sacchrp_dh_C, TPL, Thr_dehydrat_C, TnaA,
                     Urocanase]) and not (POR or POR_N or PFOR_II))"
                     /kind="single"
                     /product="TPP_AA_metabolism"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..4657
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="TPP_AA_metabolism"
                     /region_number="1"
                     /rules="(TPP_enzyme_C and not (PFL-like or PFL_6_pcresol or
                     PFL_9_tma or Gly_radical or Gly_430_pcre or Gly_8-9_TMA or
                     PFL_acetate1 or PFL_acetate2 or Gly_radical_scatole or
                     PFL_proline or PFL_toluene) and minimum(2, [ALS_ss_C,
                     AlaDh_PNT_C, Arginase, BKACE, BcrAD_BadFG, Beta_elim_lyase,
                     CPSase_L_D2, Cys_Met_Meta_PP, ELFV_dehydrog, GRDB,
                     Glu_syn_central, Glu_synthase, Gly_reductase, HAAA1, HG2B1,
                     HG2B2, HGD-D, HleuD1, HleuD2, HleuS1, HleuS2,
                     Homoserine_dh, ILVD_EDD, IlvC, Lactoyl-CoA1, Lactoyl-CoA2,
                     Lyase_aromatic, MAAL_C, OKR_DC_1_5_lysine,
                     OKR_DC_1_5_ornithine, OKR_DC_1_7_arginine, OKR_DC_1_C,
                     OTCace, PALP, Proline_reductase, Sacchrp_dh_C, TPL,
                     Thr_dehydrat_C, TnaA, Urocanase]) and not (POR or POR_N or
                     PFOR_II))"
                     /tool="gutsmash"
     CDS             2..250
                     /locus_tag="ctg232_1"
                     /transl_table=11
                     /translation="GATNTITGIADAMIDSTPIVVIAGQVGTNFLGTDAFQEVDLVGIT
                     QPITKWSYQIRRAEDVAWAVARAFYVAKSGTGRAGFC"
     CDS             234..1451
                     /gene_functions="biosynthetic (rule-based-clusters)
                     TPP_AA_metabolism: TPP_enzyme_C"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg232_2"
                     /sec_met_domain="TPP_enzyme_C (E-value: 2.5e-48, bitscore:
                     152.5, seeds: 323, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="VLDFAKNAQVEKTEYAPTKVDYVRSYLPVPEMEPEAIRKAAELIN
                     GAERPLVLVGQGVELGEAQQELRAFIEKAGMPAGCTLLGLSALPTNHPLNKGMLGMHGN
                     LGPNINTNKCDVLIAVGMRFDDRVTGKLETYAKQAKIIHFDIDPAEIDKNVKTDVAVLG
                     DCKETLAAVTQLLEPANHQEWIDSFRQYEEVEEEKVIRPELHPAGKALSMGEVVRAVSE
                     ATHNEAILVTDVGQNQMMSARYFKYSKERSIVTSGGLGTMGFGLPAAIGATFGRPDRTV
                     CVFMGDGGLQMNLQELGTIMEQKAPVKMILLNNNFLGNVRQWQAMFFNRRYSFTPMMNP
                     DYMKIASAYDIPARRVMTREELAKAIDEMIATDGPFLLEACVEEEGNVMPMTPPGGSVN
                     QMLLEC"
     CDS             1484..2044
                     /gene_functions="biosynthetic (rule-based-clusters)
                     TPP_AA_metabolism: ALS_ss_C"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg232_3"
                     /sec_met_domain="ALS_ss_C (E-value: 3.6e-20, bitscore:
                     60.8, seeds: 685, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDKKLYTIIVHSENFAGLLNQVTAVFTRRQINIESLNVSASSIKG
                     VHKYTITAWTDKDTIEKVTKQITKKIDVLQAHYFTDDEIYQHEIALYKITTPTLEEKPE
                     VSKVIRKYNARIVEVNAVFAIVEKNGMSEEITNLYEELRQLDCVLQFVRSGRVAITTSC
                     FERVNEYLADREAKYRQSKHQES"
     CDS             2041..2793
                     /locus_tag="ctg232_4"
                     /transl_table=11
                     /translation="MMSENNKIGTYKFVAEPFHVDFTGRLTMGVLGNHLLNCAGFHASD
                     RGFGIATLNEDNYTWVLSRLAVELDEMPYQYEDFSIQTWVENVYRLFTDRNFAIIDKEG
                     KKIGYARSVWAMISMNTRKPADLLALHSGSIVDYVCDEPCPIEKPSRIKVASKEPVAAL
                     VAKYSDIDINGHVNSIRYIEHILDLFPIEMYKEKRIRRFEMAYIAESYYGDELTLFMDD
                     AGEGVYDVEVKKNGSEVVCRSKVIFTEK"
     CDS             2844..3887
                     /gene_functions="biosynthetic (rule-based-clusters)
                     TPP_AA_metabolism: IlvC"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) IlvN"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg232_5"
                     /sec_met_domain="IlvN (E-value: 2.5e-47, bitscore: 149.2,
                     seeds: 43, tool: rule-based-clusters)"
                     /sec_met_domain="IlvC (E-value: 2.1e-41, bitscore: 130.3,
                     seeds: 295, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAQLNFGGVIENVMTREEFPLEKAREILKDETIAVIGYGVQGPGQ
                     ACNLRDNGFNVIVGQRPGKTYEKAVADGWVLGETLFGIEEACQKGTIVMCLLSDAAVMS
                     VWPTIKPYLTPGKALYFSHGFAITWNDRTGVVPPKDIDVIMVAPKGSGTSLRTMFLEGR
                     GLNSSYAVYQDATGKAFDKTIALGIGIGSGYLFETTFIREATSDLTGERGSLMGAIQGL
                     LLAQYEVLRENGHTPSEAFNETVEELTQSLMPLFAKNGMDWMYANCSTTAQRGALDWMT
                     PFHDAIKPVVQKLYASVKSGNEAQISIDSNSKPDYREKLNAELKALRESEMWQTAVTVR
                     KLRPENN"
     CDS             complement(3949..4656)
                     /locus_tag="ctg232_6"
                     /transl_table=11
                     /translation="LPPTIKCHEAARGGLERTLMEKVVSNKPAVVSLLKVQYRMHEEIM
                     KFPSQWFYNGELEAAPEVRYRGILDWDTPIHWIDTSEMDFKEEFVGETFGRINKAEADL
                     LLSELKIYINRIGGSRILEEKIDFGIISPYKAQVQYLRNKIKADASLKPYRSLFTVNTV
                     DGFQGQERDVIFISLVRANEEGQIGFLNDLRRMNVAITRARMKLVILGEAETLKHHGFY
                     RKLLEFIQNIGNQ"
ORIGIN
        1 gggtgctacc aatactatca cgggcattgc agatgccatg attgacagta cgcctatcgt
       61 tgtcattgcc ggccaggtag ggacgaactt cttgggtacg gatgcatttc aggaagtgga
      121 cttggtaggc attactcagc ctatcacgaa atggagttac cagattcgtc gtgcagaaga
      181 tgtggcctgg gcagtggcac gtgctttcta tgtggcaaag agcgggaccg gtcgtgctgg
      241 attttgctaa aaacgcccaa gtggagaaaa cagaatatgc gcctactaag gtggactatg
      301 tccgtagcta tcttcccgtt cccgaaatgg agccggaggc catacggaag gctgctgaat
      361 taataaatgg tgcggaacgg cctttggtgc tggtaggaca aggcgtggag ctgggagaag
      421 cccagcaaga gcttcgtgct ttcattgaga aagccggaat gcctgccgga tgcactttat
      481 tgggactttc cgcattacct acgaaccatc cgttgaacaa aggaatgctt ggtatgcacg
      541 gtaacctggg gcccaatatc aataccaata aatgtgatgt ccttatcgcc gtaggcatgc
      601 gctttgatga ccgtgtgacg ggtaaattgg aaacatatgc caagcaggcg aagattatcc
      661 actttgatat tgaccctgcc gaaatagata agaatgtaaa gaccgatgtt gctgtcctgg
      721 gagattgcaa agagacactg gctgccgtta cccaattgct ggaacccgcc aaccatcagg
      781 aatggattga cagtttccgg caatacgagg aagtggaaga ggaaaaggta atccgtccgg
      841 aattacatcc ggcaggaaaa gcgctaagca tgggtgaggt ggtgcgtgct gtgagtgagg
      901 ctacccataa cgaggcgata ttggtgactg atgtcggcca gaatcagatg atgtccgccc
      961 gttatttcaa atacagcaag gaacgtagca ttgttacttc cggtggcctg ggtactatgg
     1021 gcttcggcct tccggcagct atcggtgcca cttttggacg tccggaccgc acggtatgcg
     1081 tattcatggg agacggcggc ttgcagatga acctgcagga actgggtacc atcatggaac
     1141 aaaaggctcc ggtgaagatg attctgttga acaataactt cctgggcaat gttcgtcaat
     1201 ggcaggcgat gttcttcaat cgtcgttatt cgtttactcc gatgatgaat ccggattata
     1261 tgaagattgc ttcggcatat gatattcctg cacgcagggt aatgacccgc gaggagctgg
     1321 caaaagcgat tgacgagatg atagcgactg acggaccgtt cctgctcgaa gcttgtgtgg
     1381 aggaagaagg aaatgtaatg ccgatgacac ctccgggcgg ttcggtcaat cagatgttgt
     1441 tggaatgctg aagtcatgcg aaattaaaga acttactcta tctatggata aaaaactata
     1501 tacaatcatt gttcattcgg aaaactttgc cggtttgctg aatcaggtga cggctgtgtt
     1561 tacccgccgg cagataaaca tcgagagttt gaatgtatcg gcctcctcca tcaagggggt
     1621 acataaatat accattaccg cttggacgga caaggatacc attgaaaagg tgaccaagca
     1681 gattaccaag aaaatagatg tgctgcaagc ccattatttc acggatgatg aaatctatca
     1741 gcacgagatt gccctttata aaataacgac ccctacgctg gaagaaaaac cggaggtgtc
     1801 caaagtaatc cgtaagtaca atgcccgtat tgtggaggtg aatgctgtat ttgccattgt
     1861 tgagaagaat ggcatgagtg aggaaataac caatctttat gaggagttgc gccagttgga
     1921 ttgcgtactt cagttcgtcc gttcgggtcg cgtagccatt acgaccagct gctttgagcg
     1981 tgtcaatgaa tatctggcgg accgtgaagc aaaatatcgt caaagtaaac accaggaatc
     2041 atgatgagcg agaacaataa gataggtacc tacaagtttg ttgccgaacc gtttcatgtc
     2101 gactttaccg gacggctgac aatgggagta ttgggcaacc atttgttgaa ttgtgcgggt
     2161 ttccatgcca gcgaccgcgg ttttggcatc gcgactctaa acgaggacaa ttatacttgg
     2221 gtattgtccc gtctggctgt cgaactggat gaaatgcctt atcagtacga agatttctcc
     2281 attcagactt gggtggagaa tgtctatcgc ctttttacag accgtaattt cgctatcatc
     2341 gataaggagg gtaagaagat tgggtatgca cgttccgttt gggcgatgat tagcatgaat
     2401 acccgtaagc cggcagattt gctggctctt catagtggca gcattgtaga ttatgtttgc
     2461 gatgagcctt gcccgataga aaaaccttcg cggataaagg tggcctcaaa ggaaccggtt
     2521 gctgctttgg tagccaaata cagtgatatt gacattaacg ggcatgtgaa cagcatccgt
     2581 tacatcgagc atatccttga tttgttcccg atagagatgt ataaggagaa gcgcatccgt
     2641 cgttttgaaa tggcatatat agccgaaagc tattatgggg atgagttgac tttgttcatg
     2701 gatgatgccg gtgagggcgt ttacgatgtg gaggtgaaaa agaatggcag cgaagtggtc
     2761 tgtcgctcta aagtgatatt tacagagaaa taaataaatt tatcaacgtt ggcgtaagcc
     2821 acaaataaaa attaaaacaa aaaatggcac aattgaattt tggcggtgtt attgagaatg
     2881 taatgacccg cgaagaattt cctttggaaa aagcacgtga gattttgaaa gatgaaacaa
     2941 ttgcagtaat cggttatggc gtacaaggcc cgggccaggc ttgcaacttg cgtgataacg
     3001 gtttcaatgt gattgtagga caacgtccgg gtaagacata cgaaaaggct gtggctgacg
     3061 gatgggtact gggtgagact ttgttcggca tcgaagaggc ttgtcagaaa ggtactatcg
     3121 tcatgtgtct gttgtctgac gcagctgtaa tgtctgtatg gccgactatc aaaccttatt
     3181 tgactcccgg aaaggctctt tacttctctc atggttttgc tattacctgg aacgaccgta
     3241 ccggcgtggt tcctcctaaa gatattgatg ttattatggt tgctcccaaa ggttccggta
     3301 cttcacttcg taccatgttc ctcgaagggc gtggcttgaa ctcatcatat gccgtctatc
     3361 aggatgctac gggtaaggct tttgacaaga cgattgcact gggtatcggt atcggttcgg
     3421 gatatttgtt cgagacaact ttcatccgcg aagccacttc cgacttgacc ggtgaacgcg
     3481 gttcattgat gggtgctatc cagggattgc tgttggctca gtatgaagta ttgcgcgaaa
     3541 acggtcacac tccttctgaa gcattcaatg aaactgtgga agagttgact caatctttga
     3601 tgccattgtt tgccaagaat ggtatggact ggatgtatgc caactgttct actacggcac
     3661 agcgtggtgc gctggattgg atgaccccgt tccatgatgc catcaagccg gttgtacaga
     3721 aattgtatgc aagcgtgaag tcgggtaacg aagctcagat ttctatcgac agcaactcca
     3781 aacccgatta tcgtgagaag ctgaatgcag aattgaaagc cttgcgtgaa agcgaaatgt
     3841 ggcagacagc tgttacagtt cgtaaacttc gtccggaaaa caattaatca gctttatata
     3901 aataggtgaa ataccctctt cggtgttacc ggagggggta tttctttatt attgattccc
     3961 tatattttga ataaactcca atagcttccg gtaaaaaccg tgatgtttca gcgtctctgc
     4021 ctctcccaat atcaccagct tcatacgtgc ccgggtgatg gctacattca ttctccttaa
     4081 atcattcagg aatcctatct gcccttcttc gttggcgcgg acaaggctga tgaagataac
     4141 atcgcgctct tgcccttgga agccatccac cgtatttaca gtgaacaagc tgcgataagg
     4201 cttgagtgaa gcgtctgcct ttatcttgtt gcggagatat tgcacttgtg ccttgtaagg
     4261 ggagatgatg ccgaagtcta ttttttcttc cagaatgcgg ctgccaccga tacggttgat
     4321 gtagattttc agttctgata ggagcaggtc cgcttccgct ttattgatgc gtccgaaagt
     4381 ctctcccaca aattcttctt tgaaatccat ttcggaggtg tcaatccagt ggataggggt
     4441 atcccagtcc aggatgcccc ggtaacgcac ttcgggagcg gcttccaact cgccattata
     4501 aaaccattgg gaaggaaatt tcatgatttc ctcgtgcatg cggtattgca ctttcagcaa
     4561 agagacaacg gcaggtttgt tggacaccac tttctccatc aatgttcgtt ccagacctcc
     4621 acgggcggct tcgtggcatt ttatggtggg cggaagt
//
