LOCUS       c00399_gnlPro..         3025 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|GOLEIIAB_399.
ACCESSION   c00399_gnlPro..
VERSION     c00399_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-07 20:52:18
            Original ID  :: gnl|Prokka|GOLEIIAB_399
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 3025
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..3025
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[1:2429]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      2..2429
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..3025
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..3025
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             2..1702
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate1"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg399_1"
                     /sec_met_domain="Gly_radical (E-value: 1.1e-47, bitscore:
                     148.8, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate1 (E-value: 5.1e-262, bitscore:
                     858.0, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="LALYGIDRLIEAKQEDLRNLTGPMTEARIRLREEVAEQIKALKDI
                     KVMGEYYGLDLSHPATSAQEAVQWVYMAYLAAIKEQDGAAMSLGNVSSFLDIFIEYDLA
                     HGKINETFAQELIDQFVIKLRMVRHLRMQSYNDIFAGDPTWVTEAIGGRFNDGRVKVTK
                     TSFRFLQTLYNLGPSPEPNLTVLWSPELPEGFKDFCAKVSVDTSSIQYENDNLMREVRN
                     CDDYGIACCVSYQAIGKQIQFFGARANLAKALLLAINGGRCENTGTVMVKGIPVLTGET
                     LKFEEVMANYKKVLTEIARVYNEAMNIIHYMHDKYYYEKAQMAFIDTNPRINLAYGVAG
                     LSIAIDSLSAIKYAKVTARRNEIGLTEGFDTEGSFPCFGNNDDRVDHLGVDLVYYFSEE
                     LKKLPVYKNARPTLSLLTITSNVMYGKKTGATPDGRAKGVAFAPGANPMHGRDKNGAIA
                     SLSSVAKLRYRDSQDGISNTFSIVPKSLGPTPEERVENLVTMMDGYFTKGAHHLNVNVL
                     NREMLEDAMEHPEKYPQLTIRVSGYAVNFVKLSREHQLEVISRSFHERM"
     CDS             1704..2429
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 75.5;
                     E-value: 1.7e-23)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg399_2"
                     /sec_met_domain="Radical_SAM (E-value: 5.5e-32, bitscore:
                     99.2, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MINVHSYESMGTFDGPGLRLVVFLQGCPFRCLYCANPDTIDVKGG
                     TPTTPEEILQMAISQKAFFGKKGGITFSGGEPTLQAEALIPLFKELKANGIHICLDSNG
                     GIWNEKVEELFSLTDLVLLDIKQFNPERHRTLTGRSNEQTLRTAAWLEEHEHPFWLRYV
                     LVPGYSDSEEDIRLLGESLGKYRQIQRVEILPYHRLGVHKYEVMGWDYKLKEVKENTPE
                     QLERAEKLFKEYFTNVITN"
     CDS             complement(2498..3025)
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Fn3-like"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Glyco_hydro_3_C"
                     /gene_functions="other (smcogs) SMCOG1041:Fibronectin type
                     III-like domain (Score: 90.4; E-value: 2e-28)"
                     /gene_kind="other"
                     /locus_tag="ctg399_3"
                     /sec_met_domain="Fn3-like (E-value: 9.6e-30, bitscore:
                     90.4, seeds: 729, tool: rule-based-clusters)"
                     /sec_met_domain="Glyco_hydro_3_C (E-value: 8.5e-12,
                     bitscore: 33.1, seeds: 116, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="YNPSAKLPMTFPAHVGQIPIYYQNKSTGRPTALQQTYKASYIDAS
                     TEPAYPFGYGLSYTDFGYSDLKLLPGKDAGVHTEVSVKVTNTGKCAGEEVVQLYVQDKV
                     ASVTRPIKELKGFRKIHLNAGESKTVAFQITDDALGFYDNEMNYMVEPGDFVFMVGGSS
                     DDVQQITYNLKE"
ORIGIN
        1 attggctctc tacggcatcg accgtctgat tgaagccaag caggaggact tgcgcaacct
       61 caccggcccg atgaccgaag cacgcatccg tctacgcgaa gaagtggcgg aacaaatcaa
      121 agctttgaag gacatcaagg ttatgggcga atattacgga ctcgacctca gccatccggc
      181 tacatcagct caagaggccg tgcaatgggt atacatggca tatcttgctg ccatcaaaga
      241 gcaagacggt gctgccatgt cattgggtaa cgtatcttcg ttcctcgata tattcattga
      301 atacgatctg gctcacggaa aaatcaatga gacgtttgcg caagaactga tagaccagtt
      361 cgtcatcaag ctgcgcatgg tacgccatct gcgtatgcag tcgtacaatg atatctttgc
      421 cggtgacccg acttgggtga ccgaagccat cggaggacgc ttcaatgacg gacgcgtgaa
      481 agtcaccaag acttctttcc gcttcttgca gacgctatac aacctcggtc cttctccgga
      541 accaaacttg accgtactgt ggagtcccga actgccggaa ggcttcaagg atttctgcgc
      601 caaagtttcc gtagatacca gctccatcca gtacgaaaac gacaacctga tgcgcgaagt
      661 gcgcaactgc gacgattacg gcattgcctg ctgtgtatcc taccaagcca tcggcaagca
      721 aatccagttc ttcggcgcac gtgccaacct tgccaaggcc ctgctgcttg ccatcaacgg
      781 tggccgttgc gaaaacaccg gaactgtcat ggtgaaaggt attcccgtat tgaccggcga
      841 gacactgaag tttgaggaag ttatggccaa ctacaagaag gtactgactg aaatagcccg
      901 tgtctacaac gaggccatga atatcatcca ctacatgcat gataagtatt attatgagaa
      961 ggcccagatg gcattcatcg ataccaaccc acgcatcaat ctggcatacg gtgttgccgg
     1021 cctttccatc gccatagact cactgtccgc catcaaatat gccaaagtca ctgcacgccg
     1081 caacgaaatc ggcctgaccg aaggtttcga cactgaaggc tcattcccct gcttcggcaa
     1141 taatgacgac cgtgtggacc acctgggagt agacctcgtt tactatttca gcgaagaact
     1201 gaaaaagctg cctgtctaca agaatgcccg tcccaccctt tccttgctga ctatcacctc
     1261 caacgtgatg tacggcaaga agaccggtgc cactccggac ggacgcgcca aaggtgttgc
     1321 ctttgctccg ggtgccaacc caatgcacgg acgtgacaag aacggtgcca tcgcttcctt
     1381 gagctccgta gccaaattgc gctaccgcga ctcgcaggac ggcatcagca atacgttctc
     1441 cattgttcct aaatcactgg gcccgacacc ggaagaacgt gtagagaacc tggtaaccat
     1501 gatggacggc tacttcacca aaggtgccca ccatttgaac gtaaacgtac tgaaccgcga
     1561 aatgctggaa gatgccatgg aacatcctga aaagtatccg caactcacca tccgcgtttc
     1621 cggctatgcc gtgaacttcg tgaagttgag ccgcgagcat cagttggaag tcatcagccg
     1681 ttcattccac gaaagaatgt aatatgataa atgtacactc ctacgaaagt atgggaacat
     1741 tcgacgggcc gggtctccgg ctcgtcgttt tcctgcaagg atgtccgttc cgttgtctgt
     1801 attgtgccaa tccggatacg attgatgtaa aaggaggcac tcccaccact ccggaagaga
     1861 ttctgcaaat ggctatcagc caaaaggcat tcttcgggaa gaaaggaggt attactttct
     1921 ccggcggtga gcctaccctg caagcagaag cgctgattcc tcttttcaag gagctgaagg
     1981 cgaacggcat ccatatctgt ctggacagta acggaggcat ctggaatgaa aaggtggaag
     2041 agctctttag cctgactgac ctcgtgctgc tggacatcaa gcaattcaat cccgaacgcc
     2101 accgtacgct gacgggacga agcaacgaac agacactccg caccgctgcc tggctcgaag
     2161 aacatgaaca ccccttctgg ctgcgttatg tactggttcc cggttacagc gattcggaag
     2221 aagacatccg cctgctgggt gaaagtctcg gcaaataccg gcagatacaa cgggtggaaa
     2281 tacttcctta ccaccgtctg ggcgttcaca aatacgaagt catgggatgg gattacaaat
     2341 tgaaagaagt gaaggaaaat actccggaac aactggaacg ggcagaaaaa ctgttcaaag
     2401 agtatttcac gaatgtgata accaactaaa acagaaaagc aaggctgtcc ggatatgatt
     2461 ccagacagcc ttgcttttca tttataggac aagaatccta ttctttcaag ttataggtaa
     2521 tctgctgtac atcatcacta ctgccaccca ccataaagac gaaatcacca ggttctacca
     2581 tataattcat ctcattgtca tagaatccca gtgcatcgtc agttatctga aaagctacag
     2641 ttttgctttc acccgcattc agatgaattt tcctgaagcc cttcaactcc tttatcgggc
     2701 gcgtaacaga cgctacctta tcctgcacat acaactggac tacttcctct cctgcacact
     2761 taccggtatt agtcactttt accgacacct ccgtatggac gcctgcatcc ttgcccggca
     2821 gcagtttcaa atcagaatat ccaaaatccg tatacgaaag cccatatccg aaaggatagg
     2881 caggctcagt ggaagcatcg atataggatg ccttataagt ctgttgcaat gcggtaggac
     2941 gaccggtact cttattctgg taataaatgg gaatctgccc tacgtgtgca ggaaatgtca
     3001 tgggcaattt agccgaagga ttata
//
