LOCUS       c00354_gnlPro..         3418 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|KGFPALGI_354.
ACCESSION   c00354_gnlPro..
VERSION     c00354_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-05 15:03:36
            Original ID  :: gnl|Prokka|KGFPALGI_354
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 3418
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..3418
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[1:2587]"
                     /cutoff="10000"
                     /detection_rule="(TPP_enzyme_C and not (PFL-like or
                     PFL_6_pcresol or PFL_9_tma or Gly_radical or Gly_430_pcre
                     or Gly_8-9_TMA or PFL_acetate1 or PFL_acetate2 or
                     Gly_radical_scatole or PFL_proline or PFL_toluene) and
                     minimum(2, [ALS_ss_C, AlaDh_PNT_C, Arginase, BKACE,
                     BcrAD_BadFG, Beta_elim_lyase, CPSase_L_D2, Cys_Met_Meta_PP,
                     ELFV_dehydrog, GRDB, Glu_syn_central, Glu_synthase,
                     Gly_reductase, HAAA1, HG2B1, HG2B2, HGD-D, HleuD1, HleuD2,
                     HleuS1, HleuS2, Homoserine_dh, ILVD_EDD, IlvC,
                     Lactoyl-CoA1, Lactoyl-CoA2, Lyase_aromatic, MAAL_C,
                     OKR_DC_1_5_lysine, OKR_DC_1_5_ornithine,
                     OKR_DC_1_7_arginine, OKR_DC_1_C, OTCace, PALP,
                     Proline_reductase, Sacchrp_dh_C, TPL, Thr_dehydrat_C, TnaA,
                     Urocanase]) and not (POR or POR_N or PFOR_II))"
                     /neighbourhood="10000"
                     /product="TPP_AA_metabolism"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      2..2587
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="10000"
                     /detection_rule="(TPP_enzyme_C and not (PFL-like or
                     PFL_6_pcresol or PFL_9_tma or Gly_radical or Gly_430_pcre
                     or Gly_8-9_TMA or PFL_acetate1 or PFL_acetate2 or
                     Gly_radical_scatole or PFL_proline or PFL_toluene) and
                     minimum(2, [ALS_ss_C, AlaDh_PNT_C, Arginase, BKACE,
                     BcrAD_BadFG, Beta_elim_lyase, CPSase_L_D2, Cys_Met_Meta_PP,
                     ELFV_dehydrog, GRDB, Glu_syn_central, Glu_synthase,
                     Gly_reductase, HAAA1, HG2B1, HG2B2, HGD-D, HleuD1, HleuD2,
                     HleuS1, HleuS2, Homoserine_dh, ILVD_EDD, IlvC,
                     Lactoyl-CoA1, Lactoyl-CoA2, Lyase_aromatic, MAAL_C,
                     OKR_DC_1_5_lysine, OKR_DC_1_5_ornithine,
                     OKR_DC_1_7_arginine, OKR_DC_1_C, OTCace, PALP,
                     Proline_reductase, Sacchrp_dh_C, TPL, Thr_dehydrat_C, TnaA,
                     Urocanase]) and not (POR or POR_N or PFOR_II))"
                     /neighbourhood="10000"
                     /product="TPP_AA_metabolism"
                     /protocluster_number="1"
     cand_cluster    1..3418
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(TPP_enzyme_C and not (PFL-like or
                     PFL_6_pcresol or PFL_9_tma or Gly_radical or Gly_430_pcre
                     or Gly_8-9_TMA or PFL_acetate1 or PFL_acetate2 or
                     Gly_radical_scatole or PFL_proline or PFL_toluene) and
                     minimum(2, [ALS_ss_C, AlaDh_PNT_C, Arginase, BKACE,
                     BcrAD_BadFG, Beta_elim_lyase, CPSase_L_D2, Cys_Met_Meta_PP,
                     ELFV_dehydrog, GRDB, Glu_syn_central, Glu_synthase,
                     Gly_reductase, HAAA1, HG2B1, HG2B2, HGD-D, HleuD1, HleuD2,
                     HleuS1, HleuS2, Homoserine_dh, ILVD_EDD, IlvC,
                     Lactoyl-CoA1, Lactoyl-CoA2, Lyase_aromatic, MAAL_C,
                     OKR_DC_1_5_lysine, OKR_DC_1_5_ornithine,
                     OKR_DC_1_7_arginine, OKR_DC_1_C, OTCace, PALP,
                     Proline_reductase, Sacchrp_dh_C, TPL, Thr_dehydrat_C, TnaA,
                     Urocanase]) and not (POR or POR_N or PFOR_II))"
                     /kind="single"
                     /product="TPP_AA_metabolism"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..3418
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="TPP_AA_metabolism"
                     /region_number="1"
                     /rules="(TPP_enzyme_C and not (PFL-like or PFL_6_pcresol or
                     PFL_9_tma or Gly_radical or Gly_430_pcre or Gly_8-9_TMA or
                     PFL_acetate1 or PFL_acetate2 or Gly_radical_scatole or
                     PFL_proline or PFL_toluene) and minimum(2, [ALS_ss_C,
                     AlaDh_PNT_C, Arginase, BKACE, BcrAD_BadFG, Beta_elim_lyase,
                     CPSase_L_D2, Cys_Met_Meta_PP, ELFV_dehydrog, GRDB,
                     Glu_syn_central, Glu_synthase, Gly_reductase, HAAA1, HG2B1,
                     HG2B2, HGD-D, HleuD1, HleuD2, HleuS1, HleuS2,
                     Homoserine_dh, ILVD_EDD, IlvC, Lactoyl-CoA1, Lactoyl-CoA2,
                     Lyase_aromatic, MAAL_C, OKR_DC_1_5_lysine,
                     OKR_DC_1_5_ornithine, OKR_DC_1_7_arginine, OKR_DC_1_C,
                     OTCace, PALP, Proline_reductase, Sacchrp_dh_C, TPL,
                     Thr_dehydrat_C, TnaA, Urocanase]) and not (POR or POR_N or
                     PFOR_II))"
                     /tool="gutsmash"
     CDS             2..151
                     /gene_functions="biosynthetic (rule-based-clusters)
                     TPP_AA_metabolism: ILVD_EDD"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg354_1"
                     /sec_met_domain="ILVD_EDD (E-value: 5.7e-08, bitscore:
                     19.8, seeds: 918, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="RSINGKLTDEELAARPMTPVTRNREVSKALKAYASMVSSADKGAV
                     RLID"
     CDS             243..1994
                     /gene_functions="biosynthetic (rule-based-clusters)
                     TPP_AA_metabolism: TPP_enzyme_C"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg354_2"
                     /sec_met_domain="TPP_enzyme_C (E-value: 3.4e-48, bitscore:
                     151.4, seeds: 323, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MADNNKKSNFSSLSQGEGRGEALITGAEALMRSLEHQGVKTIFGY
                     PGGSIMPVFDALYDHRKELNHILVRHEQGATHAAQGFARVSGEVGVCLVTSGPGATNTI
                     TGIADAMIDSTPIVVIAGQVGTNFLGTDAFQEVDLVGITQPITKWSYQIRRAEDVAWAV
                     ARAFYVAKSGRPGPVVLDFAKNAQVEKTEYAPTKVDYVRSYLPVPEMEPEAIRKAAELI
                     NGAKRPLVLVGQGVELGEAQQELRAFIEKAGMPAGCTLLGLSALPTNHPLNKGMLGMHG
                     NLGPNINTNKCDVLIAVGMRFDDRVTGKLETYAKQAKIIHFDIDPAEIDKNVKTDVAVL
                     GDCKETLAAVTQLLEPANHQEWIDSFRQYEEVEEEKVIRPELHPAGKALSMGEVVRAVS
                     EATHNEAILVTDVGQNQMMSARYFKYSKERSIVTSGGLGTMGFGLPAAIGATFGRPDRT
                     VCVFMGDGGLQMNLQELGTIMEQKAPVKMILLNNNFLGNVRQWQAMFFNRRYSFTPMMN
                     PDYMKIASAYDIPARRVMTREELAKAIDEMIATDGPFLLEACVEEEGNVMPMTPPGGSV
                     NQMLLEC"
     CDS             2027..2587
                     /gene_functions="biosynthetic (rule-based-clusters)
                     TPP_AA_metabolism: ALS_ss_C"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg354_3"
                     /sec_met_domain="ALS_ss_C (E-value: 2.4e-20, bitscore:
                     60.8, seeds: 685, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDKKLYTIIVHSENFAGLLNQVTAVFTRRQINIESLNVSASSIKG
                     VHKYTITAWTDKDTIEKVTKQITKKIDVLQAHYFTDDEIYQHEIALYKITTPTLEEKPE
                     VSKVIRKYNARIVEVNAVFAIVEKNGMSEEITNLYEELRQLDCVLQFVRSGRVAITTSC
                     FERVNEYLADREAKYRQSKHQES"
     CDS             2584..3336
                     /locus_tag="ctg354_4"
                     /transl_table=11
                     /translation="MMSENNKIGTYKFVAEPFHVDFTGRLTMGVLGNHLLNCAGFHASD
                     RGFGIATLNEDNYTWVLSRLAVELDEMPYQYEDFSIQTWVENVYRLFTDRNFAIIDKEG
                     KKIGYARSVWAMISMNTRKPADLLALHSGSIVDYVCDEPCPIEKPSRIKVASKEPVAAL
                     VAKYSDIDINGHVNSIRYIEHILDLFPIEMYKEKRIRRFEMAYVAESYYGDELTLFMDD
                     AGEGVYDVEVKKNGSEVVCRSKVIFTEK"
ORIGIN
        1 ccgttctatc aatgggaagt tgacggatga agagcttgca gcacgcccta tgactccggt
       61 aacccgcaac cgtgaagtct ccaaagcatt gaaagcatat gccagtatgg tgagttccgc
      121 tgacaaaggg gctgtgaggc tgattgatta aaagcccccc tttccgaaag cgaggggagg
      181 agagaatgaa acaaattttt caagctacta ttgagctttt aaatataatg aatagataaa
      241 gtatggctga taataataaa aagagtaact tttcctccct ctcccaagga gagggtcggg
      301 gtgaggctct aataacaggc gcagaagcat tgatgcgctc tctggagcat cagggagtaa
      361 agactatttt cggttatccg ggagggagca tcatgccggt attcgatgcc ttatacgacc
      421 atcggaagga attgaaccac atcttggttc gtcatgaaca aggagcaacc catgcggcac
      481 aaggctttgc acgtgtatca ggcgaggttg gcgtttgtct ggttaccagt ggaccgggtg
      541 ctaccaatac tatcacgggc attgcagatg ccatgattga cagtacgcct atcgttgtca
      601 ttgccggcca ggtagggacg aacttcttgg gtacggatgc atttcaggaa gtggacttgg
      661 taggcattac tcagcctatc acgaaatgga gttaccagat tcgtcgtgca gaagatgtgg
      721 cctgggcagt ggcacgtgct ttctatgtgg caaagagcgg acgtccggga ccggtcgtgc
      781 tggattttgc taaaaacgcc caagtggaga aaacagaata tgcgcctact aaggtggact
      841 atgtccgtag ctatcttccc gttcccgaaa tggagccgga ggccatacgg aaggctgctg
      901 aattaataaa tggtgcgaaa cggcctttgg tgctggttgg acaaggcgtg gagctgggag
      961 aagcccagca agagcttcgt gctttcattg agaaagccgg aatgcctgcc ggatgcactt
     1021 tattgggact ttccgcatta cctacgaacc atccgttgaa caaaggaatg cttggtatgc
     1081 acggtaacct ggggcccaat atcaatacca ataaatgtga tgtccttatc gccgtaggca
     1141 tgcgctttga tgaccgtgtg acgggtaaat tggaaacata tgccaagcag gcgaagatta
     1201 tccactttga tattgaccct gccgaaatag ataagaatgt aaagactgat gttgctgtcc
     1261 tgggagattg caaagagaca ctggctgccg ttacccaatt gctggaaccc gccaaccatc
     1321 aggaatggat tgacagtttc cggcaatacg aggaagtgga agaggaaaag gtaatccgtc
     1381 cggaattaca tccggcagga aaagcgctaa gcatgggtga ggtggtgcgt gctgtgagtg
     1441 aggctaccca taacgaggcg atattggtga ctgatgtcgg ccagaatcag atgatgtccg
     1501 cccgttattt caaatacagc aaggaacgta gcattgttac ttccggtggc ctgggtacta
     1561 tgggcttcgg ccttccggca gctatcggtg ccacttttgg acgtccggac cgcacggtat
     1621 gcgtattcat gggagacggc ggcttgcaga tgaacctgca ggaactgggt accatcatgg
     1681 aacaaaaggc tccggtgaag atgattctgt tgaacaataa cttcttgggc aatgttcgtc
     1741 aatggcaggc gatgttcttc aatcgtcgtt attcgtttac tccgatgatg aatccggatt
     1801 atatgaagat tgcttcggca tatgatattc ctgcacgcag ggtaatgacc cgcgaggagc
     1861 tggcaaaagc gattgacgag atgatagcga ctgacggacc gttcctgctc gaagcttgtg
     1921 tggaggaaga aggaaatgta atgccgatga cacctccggg cggttcggtc aatcagatgt
     1981 tgttggaatg ctgaagtcat gcgaaattaa agaatttact ctatctatgg ataaaaaact
     2041 atatacaatc attgttcatt cggaaaactt tgccggtttg ctgaatcagg tgacggctgt
     2101 gtttacccgc cggcagataa acatcgagag tttgaatgta tcggcctcct ccatcaaggg
     2161 ggtacataaa tataccatta ccgcttggac ggataaggat accattgaaa aggtgaccaa
     2221 gcagattacc aagaaaatag atgtgctgca agcccattat ttcacggatg atgaaatcta
     2281 tcagcacgag attgcccttt ataaaataac gacccctacg ctggaagaaa aaccggaggt
     2341 gtccaaagta atccgtaagt acaatgcccg tattgtggag gtgaatgctg tatttgccat
     2401 tgttgagaag aatggcatga gtgaggaaat aaccaatctt tatgaggagt tgcgccagtt
     2461 ggattgcgta cttcagttcg tccgttcggg tcgcgtagcc attacgacca gctgctttga
     2521 gcgtgtcaat gaatatctgg cggaccgtga agcaaaatat cgtcaaagta aacaccagga
     2581 atcatgatga gcgagaacaa taaaataggt acctacaagt ttgttgccga accgtttcat
     2641 gtcgacttta ccggacggct gacaatggga gtattgggca accatttgtt gaattgtgcg
     2701 ggtttccatg ccagcgaccg cggttttggc atcgcgactc taaacgagga caattatact
     2761 tgggtattgt cccgtctggc tgtcgaactg gatgaaatgc cttatcagta cgaagatttc
     2821 tccattcaga cttgggtgga gaatgtctat cgccttttta cagaccgtaa tttcgctatc
     2881 atcgataagg agggtaagaa gattgggtat gcacgttccg tttgggcgat gattagcatg
     2941 aatacccgta agccggcaga tttgctggct cttcatagtg gcagcattgt agattatgtt
     3001 tgcgatgagc cttgcccgat agaaaaacct tcgcggataa aggtggcctc aaaggaaccg
     3061 gttgctgctt tggtagccaa atacagtgat attgacatta acgggcatgt gaacagcatc
     3121 cgttacatcg agcatatcct tgatttgttc ccgatagaga tgtataagga gaagcgcatc
     3181 cgtcgttttg aaatggcata tgtagccgaa agctattatg gggatgagtt gactttgttc
     3241 atggatgatg ccggtgaggg cgtttacgat gtggaggtga aaaagaatgg cagcgaagtg
     3301 gtctgtcgct ctaaagtgat atttacagag aaataaataa atttatcaac gttggcgtaa
     3361 gccacaaata aaaattaaaa caaaaaatgg cacaattgaa ttttggcggt gttattga
//
