LOCUS       c00220_gnlPro..         7048 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|HAOIBLBH_220.
ACCESSION   c00220_gnlPro..
VERSION     c00220_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-07 21:13:44
            Original ID  :: gnl|Prokka|HAOIBLBH_220
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 7048
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     CDS             1..975
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Fumarate2succinate: FAD_binding_2"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Fumarate2succinate: Succ_DH_flav_C"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1027:FAD binding domain (Score: 69.7; E-value:
                     8.2e-22)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg220_1"
                     /sec_met_domain="FAD_binding_2 (E-value: 1.1e-22, bitscore:
                     69.7, seeds: 62, tool: rule-based-clusters)"
                     /sec_met_domain="Succ_DH_flav_C (E-value: 2.5e-12,
                     bitscore: 36.0, seeds: 739, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="FFLSTNAMASNGSAAWQCYKKGAYFANPCMVQIHPTCVPVKGDYQ
                     SKLTLMSESLRNDGRIWVPKKLEDAKALQAGTKKGKDIPEADRDYYLERRYPAFGNLVP
                     RDVASRAAKERCDAGFGVNNTGLAVFLDFSDAINRLGKDVVKAKYGNLFDMYEEITNDD
                     PYETPMMIYPALHYSMGGLWVDYELMTSIPGLFAIGEANFSDHGANRLGASALMQGLAD
                     GYFVLPYTIQNYLSDQIQVPRFSTDLPEFAEAEKGIKDRIKKLMSIKGKESVDSIHKKL
                     GHIMWEHVGMARDAKGLQEAIQMLKELKKEFWSNVYIPGEPIT"
     protocluster    1..7048
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[0:2027]"
                     /cutoff="10000"
                     /detection_rule="(Succ_DH_flav_C and FAD_binding_2 and
                     (Fer2_3 or Fer4_7 or SucD_FumR))"
                     /neighbourhood="10000"
                     /product="Fumarate2succinate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      1..2027
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="10000"
                     /detection_rule="(Succ_DH_flav_C and FAD_binding_2 and
                     (Fer2_3 or Fer4_7 or SucD_FumR))"
                     /neighbourhood="10000"
                     /product="Fumarate2succinate"
                     /protocluster_number="1"
     cand_cluster    1..7048
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(Succ_DH_flav_C and FAD_binding_2 and
                     (Fer2_3 or Fer4_7 or SucD_FumR))"
                     /kind="single"
                     /product="Fumarate2succinate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..7048
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Fumarate2succinate"
                     /region_number="1"
                     /rules="(Succ_DH_flav_C and FAD_binding_2 and (Fer2_3 or
                     Fer4_7 or SucD_FumR))"
                     /tool="gutsmash"
     CDS             984..1244
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Fumarate2succinate: Succ_DH_flav_C"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg220_2"
                     /sec_met_domain="Succ_DH_flav_C (E-value: 1.2e-22,
                     bitscore: 69.4, seeds: 739, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="LDKALRLADFIEIGTLMAHDALDRAESCGGHFRTEHQTEEGEALR
                     HDDKFMYVSCWEYQGEDKDPVMLKEDLNYQFVVPQTRNYKK"
     CDS             1272..2027
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Fumarate2succinate: Fer2_3"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Fer4_8"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Fer4_4"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg220_3"
                     /sec_met_domain="Fer2_3 (E-value: 5.4e-27, bitscore: 83.2,
                     seeds: 38, tool: rule-based-clusters)"
                     /sec_met_domain="Fer4_8 (E-value: 1.8e-12, bitscore: 36.8,
                     seeds: 153, tool: rule-based-clusters)"
                     /sec_met_domain="Fer4_4 (E-value: 1.9e-06, bitscore: 17.5,
                     seeds: 79, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDKDIKVTLKVWRQKSPKDKGQFETYQIDKINQSVSFLEMLDILN
                     ERLVSEGKEPIVFDHDCREGICGMCSLYVNGHPHGPATGATTCQLYMRRFNDGETITIE
                     PWRSAGFPVIRDLMVDRYAYDKIIQAGGFVSVNTGGVPDANSIAIPRADAEMAMDAAAC
                     IGCGACAAACKNGSAMLFVSAKVSQLALLPQGKVEAARRAKAMVAKMDELGFGNCTNTR
                     ACEMECPKNISVSNIARLNREFICAKLQD"
     CDS             complement(2326..3171)
                     /locus_tag="ctg220_4"
                     /transl_table=11
                     /translation="MEDNIPKFDLPVDFIVGNSITGEILNQYGRFPCKIKAGIFVLCVQ
                     GTVHATVNLTEFTIRKNDFITLPPGCFIQIHEVSDDVKLCFAGFSSVFVSHINYIKTIT
                     NYLPVIFDSPIMPLPEHISLLYQQAFDLLINAYSMPKTIENKEIIKAIFTIFMQGVIEL
                     YRNHTPYSNAPMTRNMEICREFVQLAMENYTKEHSVSYYAKRLNITLQHFCYVIKKVSG
                     RTALDILSNIIIMDAKAQLKSTDMPVKKIAFALGFDNLSFFNKYFRQHVGMTPQEYRES
                     "
     CDS             3412..4626
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Radical_SAM"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg220_5"
                     /sec_met_domain="Radical_SAM (E-value: 1.1e-14, bitscore:
                     44.2, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSTKRTKSSFRTMSTNRKLESVFLFVTGRCNAKCKMCFYAEDMDK
                     KEKDLTFEEIKTISETAGQFNRLWVSGGEPTLREELPEIIEMFYKNNEIKDVNIPTNGL
                     KPDRIVAWVKQIRQNCPELNINLSLSLDGFGDTHDIQRGVPGNFYKAMGTLKLIEKNFA
                     DDGRVLKNLATVITKYNENEVEDLMIWMLGRFHLSNHIIEAARGMTRDDGVKVLTEPTL
                     RALQDKVVPIYNVYGERMAEETGGFRKPIARFFYLGFIRTLYNVRATNIDHPTPWGMDC
                     TAGETTLVIDYDGRFRACELREPLGNVKDYDCNIAKIMESDAMKQEIAAIGHGDKANCW
                     CTHGCWITSSLVFNPRKMITQVYKGYREMKKLNRPLNISEELLQSIERKYALDEQKLVE
                     LGVRK"
     CDS             4804..5388
                     /locus_tag="ctg220_6"
                     /transl_table=11
                     /translation="MTTKLKDLFDTFLNIAASSQKLGINLIRVAILIIFVWIGGLKFYN
                     YEAEGIVPFVANSPFMSFFYAKSAPEYKEYKLKEGEFNEAKHQWHVENNTYIFSHGLGI
                     MIMAIGILTFLGIFSPKIGLIGSGLAIIMTLGTLSFLVTTPEVWVPDLGSGEHGFPLLT
                     GAGRLVIKDTAIIAGAIVVLSDCAQRILKKK"
     CDS             5794..6543
                     /locus_tag="ctg220_7"
                     /transl_table=11
                     /translation="MNLRKNLFWAIPFIVVGVVWIMRNTGMITDDLFHIIVSWQMLVMY
                     IGIGSISRRHYIGGLITFLVGAAFFLPRLGWMDSDWLQVYWPAAFVIVGLMLIFEPRKK
                     NSYHYNSRREDTGGEDIRGNAVAGKVKSDFINTDGYVESDNTFGYVEQIILDPVFKGAR
                     IRTVFGGTVLDLRKTKLDSAKTYIDIDCKFAGVEIYLPGDWAVNFHGSPIMGACEDKRF
                     NASMEMDKEHILVVRGIVMFGGIEFKS"
     CDS             6546..7046
                     /locus_tag="ctg220_8"
                     /transl_table=11
                     /translation="MQTHPFINSVLCRMAGVALAVVSCSISALLLYYYGHTGWLVAWLD
                     SLVSVGIFCVSGFLFWYVTGILRPLQAQIALAVGVLIISVTGGYMAGALVYPDRMDWFA
                     STIPFRILLGLLGWIILAQWYYIRWKELQMEIQEEQRKEENAPAETGVSSPEEEAILDR
                     ISVK"
ORIGIN
        1 ttcttcctgt ctactaacgc aatggcttcc aacggttctg ctgcatggca gtgctacaag
       61 aaaggtgctt acttcgctaa cccttgtatg gtgcagatcc acccgacttg tgtaccggtg
      121 aaaggtgact accagtctaa gctgactttg atgtctgaat cattgcgtaa cgacggtcgt
      181 atctgggttc cgaagaaact ggaagatgca aaagcattac aggccggaac taagaaaggg
      241 aaagacattc ctgaagcaga ccgcgactac tatctggaac gccgttatcc ggcattcggt
      301 aacctggttc cgcgtgacgt ggcttcacgt gccgctaaag aacgttgcga cgccggcttc
      361 ggtgtgaaca acacaggtct ggccgtgttc ctcgacttct ccgacgccat caaccgtctg
      421 ggtaaagatg ttgtaaaagc taaatatggt aacttgttcg acatgtacga agaaattacc
      481 aacgacgatc catacgaaac tccgatgatg atttatcctg cattgcacta ctcgatgggt
      541 ggtctgtggg ttgactatga attgatgact tctattccgg gtctgttcgc tatcggtgaa
      601 gctaacttct ctgaccacgg agctaaccgt ctgggtgctt ctgcgttgat gcagggattg
      661 gccgatggct actttgtatt gccttataca attcagaact atctgtcaga ccagatccag
      721 gttccacgtt tcagcaccga cctgcctgag tttgcagaag ctgaaaaggg gatcaaagac
      781 cgcatcaaga aattgatgag tatcaagggt aaagagtcgg tagattctat ccataagaaa
      841 ctgggtcata tcatgtggga acatgtaggt atggcacgtg atgcaaaagg tttgcaggaa
      901 gcgatccaga tgttgaaaga gctgaagaaa gagttctgga gtaatgtata tattccgggt
      961 gagccgataa cctgaacgta gagttggata aggcattgcg tttggctgac tttattgaga
     1021 ttggtacatt gatggcacac gatgcattgg accgtgccga atcttgtgga ggacacttcc
     1081 gtacagagca ccagaccgag gaaggcgaag ccctgcgtca tgacgataag ttcatgtacg
     1141 tatcttgttg ggaataccag ggtgaagaca aagatccggt tatgctgaag gaagatttga
     1201 actatcagtt cgttgttcct caaacacgta actacaagaa gtaataatca tcaatcacaa
     1261 aaattagaat catggataaa gatataaaag taacacttaa agtttggcgt cagaaaagtc
     1321 cgaaagataa aggacaattt gagacttacc aaatagacaa aatcaatcag agtgtttctt
     1381 tcctggaaat gttagacatt ttgaatgaac gtttggtcag tgaaggtaaa gagccgatcg
     1441 tattcgatca cgactgtcgt gaaggtatct gcggtatgtg ttctttgtat gtaaacggac
     1501 atccgcacgg acctgcaacc ggcgcaacta cttgccagct ttatatgcgt cgcttcaatg
     1561 atggcgaaac tattacaatc gaaccgtggc gttctgccgg tttcccggta atccgcgact
     1621 tgatggtaga ccgttatgct tacgataaaa ttatccaggc aggtggtttc gtatcagtga
     1681 acacaggtgg tgtacctgat gccaattcaa tcgctattcc tcgtgcagat gctgaaatgg
     1741 ctatggatgc tgctgcatgt atcggttgcg gtgcttgtgc tgctgcttgt aagaacggtt
     1801 cggctatgtt gttcgtttct gcaaaggtta gccagttggc tctgttgcct cagggtaagg
     1861 tagaagctgc tcgtcgtgcg aaagcaatgg ttgccaagat ggacgaactg ggcttcggta
     1921 actgtaccaa cacgcgtgct tgcgaaatgg aatgtccgaa gaacatctct gtcagcaaca
     1981 tcgctcgttt gaaccgcgag ttcatttgtg ctaaattgca agactaatat acattatata
     2041 tgtatgtccg gagaggcgtt gcttatggca gcgcctcttc ttttttgccc ctattttaac
     2101 atttctgaag tcatagataa atatcttgaa tgttgttagt gtttcttata tgctatacaa
     2161 cactaattag cattttaact attgacaagt tggagaatac ctgtatcttt gtactgtggt
     2221 tttttcataa tagtattaga tttaaggtta acaaagttgg ttagggcttg tcgtgagaca
     2281 ggctttaatt gtttataggg gtttgcgtcc ggcatatccc tttttttagc tctccctgta
     2341 ttcctgcggg gtcattccta catgttgccg gaaatatttg ttgaagaacg aaaggttgtc
     2401 gaaacccagg gcgaatgcaa ttttcttgac cggcatatcg gtcgatttaa gctgcgcttt
     2461 ggcatccata atgatgatat tgctcaggat atccaatgca gtccgtccgc tgaccttttt
     2521 tatcacataa cagaaatgct gcaaggtgat gttgagcctt ttggcgtaat aagacacgct
     2581 atgctctttc gtgtagtttt ccatcgccag ttgtacgaac tcccggcata tttccatgtt
     2641 acgcgtcatg ggagcattgc tatatggcgt atgattcctg tataattcaa taaccccttg
     2701 catgaagata gtaaagattg ccttgataat ctccttgttc tcgatggttt taggcatgga
     2761 ataagcattg atcagcaagt cgaaagcctg ttggtagagt aaggatatat gttccggcag
     2821 aggcatgatc ggactgtcga atatgacagg cagatagttc gtaatcgtct ttatataatt
     2881 gatatggctg acgaatacgg aagaaaagcc tgcaaaacac agttttacat catcggaaac
     2941 ctcatgtatc tgaatgaagc aacccggggg taaggtgata aagtcgttct tccggatggt
     3001 aaactcagtt aagtttacag tcgcgtggac tgtcccttgt acgcaaagca cgaatatgcc
     3061 agccttgatc ttgcacggga aacgtccgta ctggttcagt atttcacctg taatactgtt
     3121 tcctacaata aaatctacgg ggaggtcgaa tttaggaata ttgtcttcca tacttttata
     3181 tacacttaat tgaatgtaca aagatagttt ttttgagaca agttagtcga taatcttaaa
     3241 tatagaacat cgaatcccac aaatggagat tgttttgtgc tgtataatag ttgaaatttg
     3301 cgaatgtttt caagtcttgc tgatagattt gttgggatgg tatatatttg tctatctgct
     3361 aaataagata tgggagctaa tactaataga gaaatctaat cgataataac aatgagtacg
     3421 aaaagaacaa agagctcttt caggacaatg agcacaaaca gaaaattaga atctgttttc
     3481 ctgtttgtca caggacggtg taatgctaaa tgtaaaatgt gtttctatgc agaagacatg
     3541 gataaaaagg agaaagacct gacttttgaa gaaatcaaga caatctccga aacagccggg
     3601 cagttcaacc gtttgtgggt atccggcggt gaaccgacac tgcgtgagga gttacctgaa
     3661 attatagaga tgttctacaa gaataacgag ataaaggacg tgaatatccc taccaacgga
     3721 ttgaagcctg accggatcgt cgcctgggta aaacagatcc gccagaattg tccggaactg
     3781 aatatcaacc tgagcctttc tttggacggt ttcggtgata cgcatgatat acagcgcggc
     3841 gttcccggca atttctacaa agcgatgggg acactgaagc tgattgaaaa aaactttgct
     3901 gacgacggca gggtgttgaa aaacctggcc accgtaatca ccaaatataa cgagaatgag
     3961 gtcgaggatc tgatgatatg gatgctggga cgtttccatc tttccaatca tattattgaa
     4021 gctgcacgcg gtatgacccg cgacgacgga gtgaaggttc ttaccgagcc tactctacgc
     4081 gccttgcagg ataaggttgt tcctatttat aatgtatatg gggagcgtat ggcggaggaa
     4141 accggtgggt tcagaaaacc tatcgcccgt ttcttctacc tgggtttcat acgtacatta
     4201 tataatgtac gtgcgaccaa tatcgatcat ccgacaccct ggggtatgga ttgtacggcc
     4261 ggcgaaacaa cactggtaat agactatgac ggtcgtttcc gtgcctgtga acttcgcgaa
     4321 ccgctcggta atgtcaagga ttatgactgc aatatcgcaa agattatgga gagcgatgcc
     4381 atgaaacaag agattgccgc tatcggtcat ggtgacaagg ctaactgctg gtgtacgcat
     4441 gggtgttgga tcacttcatc gcttgttttc aatccccgca agatgatcac gcaggtctat
     4501 aaaggatacc gggagatgaa gaagctgaac cgtcctctga atatcagtga agagcttttg
     4561 cagagtatcg aacgcaaata tgccttggac gaacagaaac tggtggagct gggagttcgt
     4621 aagtaaaaga gtgccgataa cggatcattt ataaagccgg ttgacgggaa atatacctct
     4681 gttaaccggc tttattgtgt ttaagggaat aattgtcaaa ttatccggta gaattgccat
     4741 ttcccatagg ggaaaaatgt cggatctttg catcataagt taaactgata aacaaagaaa
     4801 gacatgacta ctaaattaaa agatttattc gacacatttc tgaacattgc agcttcttca
     4861 cagaaactgg gtattaacct gatccgcgta gctatcctga ttatattcgt atggatcggc
     4921 gggctgaaat tctataatta tgaagcggaa gggatcgttc cttttgtggc aaacagtccg
     4981 tttatgagct ttttctatgc aaagagtgca ccggaataca aagaatataa gttgaaagaa
     5041 ggtgaattca atgaagccaa gcatcagtgg catgtagaaa acaataccta tattttttca
     5101 catggactag gcattatgat catggcgatc ggtatcctga ctttcctggg tatcttttct
     5161 ccgaaaattg gcttgatagg ttccggactg gcgatcataa tgacattggg tacgctttca
     5221 ttcctggtga caacacctga agtgtgggtg cccgatttgg gaagcggaga acatggtttc
     5281 cccttactga ccggagcagg tcggctggta ataaaagata cggcaatcat agccggagcg
     5341 atcgtggtat tgtccgactg tgcacaacgt atattaaaga agaaatagga ataaaaagtg
     5401 tttattttaa cactttggaa gcaaaaaaca aacatattga accttgttag cttttcttat
     5461 agtgtatgaa acaccattta gcataaaacc tcttgacagg tttggattaa gcagtatatt
     5521 tgcatcgtgg ttttttcata atagtattag atttaaggtt aacaaagttg gttagggctt
     5581 gtcgtgagac aggctttaat tgtttatagg ggtttgcatt cggaaatgca agcctttttt
     5641 cttttaaata tggcattctc tgaaaaataa ctcccttttt tatcggtaat attcggcaat
     5701 tcaccgatat ttgactgtaa taggacgtat ttctattgcc ggaaggctga caggagcata
     5761 cttttgctct caaacaatta aactgtatat cgtatgaatc ttaggaaaaa cttattttgg
     5821 gccatcccgt ttattgtagt gggggtcgta tggatcatga ggaacacagg catgatcacg
     5881 gatgatcttt ttcatatcat tgtatcctgg cagatgcttg taatgtatat aggtataggg
     5941 agtatttccc ggagacatta tataggcggt ttgattacct ttttggttgg tgcggcattt
     6001 ttcctgccac gactggggtg gatggatagc gattggttgc aggtctattg gccggctgct
     6061 tttgtcattg tcggtttaat gctgatcttc gaaccgcgta aaaagaactc ctatcattac
     6121 aatagccgga gagaggatac cggtggagaa gatatccggg gaaatgccgt tgccggtaaa
     6181 gtaaaaagtg attttattaa tacggacgga tatgtggaat cggataatac atttggctat
     6241 gtggaacaaa ttattttaga tccggtattt aaaggagcca ggattaggac tgtgtttggg
     6301 ggaacggtcc tggatttgcg gaaaacgaag ctggatagtg ctaaaaccta tattgatatc
     6361 gattgtaagt ttgccggtgt ggaaatctat cttcctgggg attgggcggt caattttcat
     6421 ggcagtccga tcatgggggc atgcgaggat aaacgtttta atgcctctat ggaaatggat
     6481 aaagaacata tactggttgt acggggaatt gttatgttcg gtggcattga atttaagagt
     6541 tgattatgca gacacatccg tttataaatt ctgtcctgtg tagaatggct ggtgtggctt
     6601 tggctgtggt cagttgcagc ataagcgcct tgctcctcta ttattacgga catacgggat
     6661 ggcttgtcgc atggttggat agtctggttt cggtcggtat attctgtgtc tccggtttcc
     6721 tgttctggta tgtaacaggc atattgcggc cattacaggc ccagattgca ttggctgttg
     6781 gcgtccttat catatccgtt accggaggat atatggccgg ggcgctggtt tatccggata
     6841 ggatggattg gtttgcatct accattccgt tccgtatttt gttgggtttg ctgggatgga
     6901 ttattttggc ccagtggtat tatatccgtt ggaaggagtt gcagatggag atacaggagg
     6961 aacagagaaa agaagaaaat gctccggcgg aaacaggcgt atcgtcaccg gaagaggaag
     7021 caatactgga tcgcatctct gtgaagga
//
