LOCUS       c00366_gnlPro..         3563 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|IOOEDBAK_366.
ACCESSION   c00366_gnlPro..
VERSION     c00366_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-05 13:21:14
            Original ID  :: gnl|Prokka|IOOEDBAK_366
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 3563
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..3563
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[569:3545]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      570..3545
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..3563
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..3563
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             complement(3..371)
                     /locus_tag="ctg366_1"
                     /transl_table=11
                     /translation="MKTPQEIAEMISKRFYPLPDADLDSLASIIESSKAAKGELLLDNG
                     QISKYLYYVESGLLRQFYYKNKRDITEHFSCEGNIVICIASTFQREPTRLLIEALEPTV
                     YHRISFSEFSDLLKTSLAI"
     CDS             complement(570..1304)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 66.9;
                     E-value: 7.6e-21)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg366_2"
                     /sec_met_domain="Radical_SAM (E-value: 5.4e-34, bitscore:
                     105.7, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="VIKGKIHSLESFGTVDGPGIRFVVFMQGCPLRCLYCHNPDTWTPK
                     GKVKYQMTPGELLTEVLRYKSFIARGGVTVTGGEPLLQPEFLKEFFRLCQEQGLHTALD
                     TSGFVCTSKAWEVLDYADLVLLDIKTLNPDLHPLLAGVKQDNTLLFLDELERRGIDTWI
                     RHVIVPGYTDNDEWLEALARYVSSYKVVRKVELLSYHTMGTYKYEQLGLDYPLKGVEPL
                     SKERLDNAKAIFSRYKPENNKA"
     CDS             complement(1305..3545)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate2"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg366_3"
                     /sec_met_domain="Gly_radical (E-value: 1.2e-46, bitscore:
                     145.5, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate2 (E-value: 1.4e-283, bitscore:
                     929.4, seeds: 33, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKFENECWDHFKGETWKREINVRDFIQSNYTPYEGDDSFLVASSE
                     KTRKVWNKLTEMFKVEREKGVYDVETKLPQGIDVYGPGYIDKENEVIVGLQTDAPLKRG
                     IFPKGGIRMVENSLEAYGYHLDPMTKEIFTKYRKTHNEGVFSAYTEEMIAARRSAIITG
                     LPDAYGRGRIIGDYRRVALYGTAILIEEKKRFLNRLDIQEITEEIIQSREEISEQIKAL
                     KAFEKMCASYGFDVTRPAQNAREAVQFVYLAYLAAVKDQDGAAMSIGRTSTFLDIYIEK
                     DIREGKLTEEEAQELVDQLIIKLRIVRFLRTPEYNDLFSGDPVWVTESLGGQGVDGRSL
                     VTRTSYRYLHTLYNLGPAPEPNLTVLWFKNAPENWKRFCAKVSIDTSAIQYENDDLMRP
                     DYGDDYGIACCVSPMKIGKQMQFFGARANLAKCLLYAINGGRDERSGVQVAPMFEPVRG
                     EYLEYDEVMAKYEQMMRWLAKVYVNALKIIHYMHDKYAYEAFEMSLHDGDVERIRATGI
                     AGLSIVADSLAAIRDTKVRVIRDERGLAVDFEREGEYVPFGNNDDRTDSIAVEITEKFM
                     EYLRQHETYRHAKPTQSILTITSNVVYGKKTGTTPDGRIGGTPFAPGANPMNGRDTKGA
                     IAALASVAKLPFQHAHDGISYTFAVSPATLGKEREVQINNLVSLLDGYFTPDGGQHLNV
                     NVFDKDLLIDAMEHPEKYPQLTIRVSGYAVNFVKLTREQQLDVISRTINHSL"
ORIGIN
        1 ttaatagcta aagatgtttt caataaatcg ctaaattccg aaaaagagat ccggtgataa
       61 accgtcggtt ccaatgcctc gattaataaa cgcgtcggtt ctctttggaa tgtgctggct
      121 atacaaataa cgatatttcc ctcacaggag aaatgctcgg taatatcacg tttattctta
      181 tagtaaaact ggcgtaataa tcccgattcc acataatata aatatttaga tatttgtccg
      241 ttatccaaca ataattctcc tttcgcagcc ttacttgatt ctatgatcga agccagagaa
      301 tccaaatccg catcgggtaa tggataaaaa cgtttggata tcatttcggc tatttcttgt
      361 ggtgtcttca tactattcat cattttctga tctatatcat tttttatctc cttacaaaaa
      421 tagatatttg ttgcatataa caaaacaaac acatattaaa tagtcaaggt tagtctaggc
      481 aaaaaattat tactatcgtt taaaagctaa tcggattaga ttgttttccc tgattagctg
      541 ttgcataatc gcaaggcttg atcgagagat caggccttgt tattttctgg tttgtagcga
      601 gagaatatcg ctttcgcatt atctaaacgt tcttttgata agggttccac cccctttaac
      661 ggataatcaa ggcctaattg ctcatattta taggttccca tcgtatgata agacaataat
      721 tccactttgc gaaccacttt atatgaactg acataccgag ctaacgcctc cagccactcg
      781 tcattatccg tatatccggg gacaataaca tggcgaatcc aagtatctat gccacgtctc
      841 tccaattcat ccaagaacag aagcgtatta tcctgcttga cacccgccag taacggatgt
      901 aaatccgggt ttaaggtttt gatgtccaat aacactaagt ccgcataatc caagacttcc
      961 caagcttttg aggtacatac gaaaccagat gtgtccaaag ctgtgtgaag accttgctct
     1021 tggcacaaac ggaaaaactc tttcagaaac tcaggttgca ataaaggttc cccaccggta
     1081 acagtcaccc ccccacgggc gataaaactc ttataccgta agacttccgt caatagctcg
     1141 ccgggagtca tctgatattt caccttccct ttcggggtcc aagtatccgg gttatggcaa
     1201 tacaggcaac gcaacgggca gccctgcata aaaacgacaa agcgaattcc gggaccatct
     1261 acggtcccga aactctctaa tgaatgtatc ttacctttta tcacttacag tgaatggtta
     1321 atggttcttg aaataacatc caactgttgt tcacgagtca gtttcacgaa gtttaccgca
     1381 tatccggata cacggatcgt aagttgcgga tatttctcgg gatgctccat agcgtcgatc
     1441 aacaaatcct tatcaaatac gttaacattc agatgctgac ctccatccgg agtaaaatag
     1501 ccatccaata agctaaccag attattgatc tgcacttcac gctcctttcc caacgtagcc
     1561 ggagaaacag cgaaagtata agagataccg tcgtgcgcat gctggaatgg aagcttcgct
     1621 accgaagcta gcgcagcgat agcacctttc gtatcacgac cgttcatagg gttcgcgccc
     1681 ggagcgaaag gagttccacc gatacggcca tccggtgtcg taccagtctt cttaccatat
     1741 accacattag aggtgatcgt aaggatggac tgcgtaggtt tcgcatggcg ataggtctcg
     1801 tgctgacgca aatattccat gaacttctcg gtgatctcta cagcgatact atccgtacga
     1861 tcatcgttat taccgaaagg aacatattcg ccctcacgtt cgaaatcaac cgccaagcca
     1921 cgctcgtcac ggatcacacg tactttcgta tcacggatag cagccaacga atcggcaacg
     1981 atagaaagac cggcgatacc tgtcgcacgg atacgctcta catccccatc gtgaagcgac
     2041 atctcgaatg cctcatacgc atatttatca tgcatataat ggattatctt caaggcgttc
     2101 acatacactt tcgccaacca gcgcatcatt tgctcatact tagccatcac ctcatcgtat
     2161 tccagatact cgccgcgaac cggctcgaac atcggggcta cctgtacgcc gctgcgctca
     2221 tcccgtcctc cgttgatcgc atataataaa catttcgcca aattcgcacg ggctccgaag
     2281 aactgcatct gcttaccgat cttcatagga gaaacgcaac aagcgatacc ataatcatct
     2341 ccataatccg gacgcatcaa atcgtcgttc tcatattgga tagccgatgt atcgatagag
     2401 accttggcac agaagcgttt ccagttctcg ggagcgttct taaaccacag aactgtcaaa
     2461 ttgggttctg gtgccggacc tagattatat aatgtatgga gataacgata ggaagttctt
     2521 gttaccaaag aacggccatc cactccttgg ccgcccaagg actctgttac ccaaaccgga
     2581 tcaccggaga acaaatcatt gtattccggc gtacgtaaga aacgaacgat acgcaacttg
     2641 atgatcagct ggtctaccaa ttcttgcgcc tcctcttcgg tcaacttacc ctcacggatg
     2701 tctttctcga tataaatatc caaaaacgta gaagtacgtc cgatagacat cgccgcgcca
     2761 tcttggtctt ttaccgccgc cagataagct aagtacacaa actgaaccgc ctcacgagcg
     2821 ttttgtgccg gacgagtcac atcaaagcca tagctagcgc acattttctc gaacgctttc
     2881 aaagccttga tctgttcgga aatctcctca cggctctgga tgatctcttc cgtgatctct
     2941 tggatatcca aacgattcaa gaaacgcttt ttctcctcga tcaagattgc cgtaccatat
     3001 aaagcgacac gacgatagtc accaataata cgtcctctac catacgcatc gggcagaccg
     3061 gtgataatag ccgaacgacg tgcggcaatc atctcttccg tatatgcgga gaaaacgccc
     3121 tcgttatgcg tcttacgata cttcgtgaag atctctttcg tcatcgggtc caaatgataa
     3181 ccataagctt ccaagctatt ctcaaccata cggataccgc ccttcgggaa gataccacgt
     3241 ttcagcggag catcggtttg taacccaacg ataacctcat tctccttatc aatatagccc
     3301 gggccatata catcgatccc ttgaggtaat tttgtctcga cgtcataaac tcccttttcc
     3361 ctctcgacct taaacatctc ggtcagcttg ttccagacct tacgggtctt ctcggaagaa
     3421 gcgactaaaa aggaatcatc cccttcataa ggcgtatagt tactctggat aaaatctctt
     3481 acattgatct ctcttttcca ggtctcaccc ttaaagtgat cccagcactc attctcaaat
     3541 ttcataaaca atcttatcta ata
//
