LOCUS       c00122_gnlPro..         5839 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|ANEGPLJG_122.
ACCESSION   c00122_gnlPro..
VERSION     c00122_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-07 21:18:26
            Original ID  :: gnl|Prokka|ANEGPLJG_122
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 5839
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..5839
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[2581:5645]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      2582..5645
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..5839
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..5839
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             complement(3..422)
                     /locus_tag="ctg122_1"
                     /transl_table=11
                     /translation="MKNLLFALLTGSFCCCYAQQKAAPVPEPEVVATPPADAGRGLIRV
                     DSREIRHYSGTRKEPDYLVSRDNGKTWEMKAAPAGYPPNYGGIPKESPAIVRNPLTREF
                     IRVQPIGGFVFLSRGGLDGKWLAVTNDGKLEEDWKD"
     CDS             complement(507..1295)
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Lipase_GDSL_2"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg122_2"
                     /sec_met_domain="Lipase_GDSL_2 (E-value: 1.7e-23, bitscore:
                     72.4, seeds: 1564, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNFLSYMLAILLGSSSLAFSAPSIPASALPVTLKPEPHQRDPYDW
                     NARHEAVKKRNRAVKPEYVMIGDSITHHWGGEPSGDGKNTGSGSWEKLFGPHAATNMGF
                     GFDYVDNAYYRVQNGELDGISPRVIIVLLGTNNLGHRKDTPQACADNIRAFVRLVRRKC
                     PSSKILLLGVLPRKEKNLAEPVKQTNRLLAGLHNGKDIFFADPGKALLSADGASPQNEF
                     MKDVVHPNARGYEVLEKELAVLLKKLDAKYRGGKQAGKHS"
     CDS             complement(1519..2301)
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Lipase_GDSL_2"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg122_3"
                     /sec_met_domain="Lipase_GDSL_2 (E-value: 8e-25, bitscore:
                     76.7, seeds: 1564, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKSYACLCLLLAGVFCVACFSGACADELPPTLKPQVNPRAVYDWK
                     KRHEAVMERNRTVKPEYVVFGDSITHRWGGEPSGDNRALGTGKTAWDSLFSSHAVTNMG
                     FGSDYVDNAYYRLQLGELDGISPRVVIVLLGTNNLGGRKDAPRACADNLKAFVSLARRK
                     CPSSKILLLGILPREEKKLAPLIRETNKMISGLADGNSVFFANPGEEFLGEDGVSPKPG
                     LLEDGLHPSARGYDILGKSLSVVLKKMDDKYGKLPAPR"
     CDS             2582..4849
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate2"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg122_4"
                     /sec_met_domain="Gly_radical (E-value: 2.2e-46, bitscore:
                     145.3, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate2 (E-value: 3.8e-282, bitscore:
                     925.4, seeds: 33, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MISIVKDLKESTALPQEWQGFKPGAWTESIDVRDFIQHNYTPYSG
                     NEEFLSGPSQRTLRLWDELKVLLKREIDNGGVLDADEEVVSSITSHKPGYIDKELEVVV
                     GLQTDAPLKRALMPFGGLRMAQQALESYGFKMCEKTADIFKKIRKTHNEGVFDAYTSDI
                     RAARSAGIITGLPDAYGRGRIIGDYRRVALYGTDKLIAERRKDLKNREHSPLTDDLIRL
                     REEMSEQIRALEELAQLGASYGCDLTRPAANAREAVQWTYLGYLAAVKEQNGAAMSLGR
                     VSTFFDIYFTRDLEQGLITEEEVQEIIDQFVMKLRIVRFIRTPDYNNLFSGDPTWVTES
                     IGGMGEDGRTLVTRSSFRMLQTLYNLGPAPEPNLTVLWSRNLPEAFKSFCAKVSIETSS
                     VQYENDDLMRPHWGDDYGIACCVSAMRIGKQMQFFGARANLAKCLLYALNGGVDELKGK
                     QVAPPSPRYTEEILNYDEVMALYDKMQDWLAKTYIDALNIIHYMHDKYCYERIEMALHD
                     PEILRTMATGIAGLSVAADSLSAIKYATVKAIRNEEGLIVDFKTEGEFPCYGNNDPRVD
                     DIACSLVSNFMEKLRRLHTYRNSLPTQSILTITSNVVYGKKTGNTPDGRRAGEPFAPGA
                     NPMHGRDRNGAVASMLSVAKLSYDDSLDGISYTFSIVPQALGKEERERRVKLVSLLDAY
                     FAATGHHINVNVLERETLLDAMDHPEKYPQLTIRVSGYAVNFIKLTREQQQEVINRTFH
                     TR"
     CDS             4866..5645
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 54.8;
                     E-value: 4.1e-17)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg122_5"
                     /sec_met_domain="Radical_SAM (E-value: 8.3e-32, bitscore:
                     99.3, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPQFFPQNHAPDGESSVTGLVHSVESCGTVDGPGIRFVLFLSGCS
                     LRCRYCHNPDTSYVRRGRLRSADDVLKEIARYRDFLQAAGGGVTLSGGDPLFQPDFTGA
                     VLKGCRKLGLHTCLDTSGHLSVNAGEEMLADTDLVLLDIKAWNPKRYRALTGGELRPTL
                     EFAERLAPLRKPVWLRYVLVPGVTDNLEEIEELSRHAALLGNVERVDVLPFHQLGRFKW
                     DELKLDYTLKDVPEASAELAEQVRGIFRRGGFPACVH"
ORIGIN
        1 gggtctttcc agtcttcttc cagtttgccg tcattcgtga cggcaagcca cttgccgtcc
       61 agcccacccc tggaaagaaa tacaaagccc ccgataggct gcacacgaat gaattccctg
      121 gtcaaagggt tgcgcacaat agccggagat tccttgggaa tgccaccgta gttgggaggg
      181 tagccagccg gagcggcctt catttcccat gtttttccgt tatccctgct gaccaggtaa
      241 tccggttcct tgcgggtacc ggaataatgg cgtatttcac gggagtccac acggataagg
      301 ccgcgccccg catcagccgg cggagtggcg acaacttcag gctccggaac gggtgcggcc
      361 ttctgttggg cataacagca acaaaaggaa ccggtcaaca atgcaaacaa caggtttttc
      421 ataaactaaa tatcaaaggc cgtatatttt cctcaaccgg ggctttccct tcaagcaaaa
      481 tcctaccttc catcagaaga caatcatcag gaatgctttc ccgcctgctt ccctccccgg
      541 tattttgcat caagcttttt caggagtacg gccagttcct tttccaaaac ctcgtacccc
      601 ctggcattgg gatgcaccac atccttcatg aattcattct gcggagaagc accgtctgcg
      661 gaaagaagcg cctttcccgg atcggcaaag aaaatgtcct ttccgttatg cagccccgcc
      721 aacagtctgt tcgtctgctt caccggttcc gccaggtttt tctccttccg tgggagaacg
      781 cccagcaaca ggattttgga agaggggcac ttccggcgca ccaggcggac aaatgccctg
      841 atattgtccg cacatgcctg cggcgtatcc ttgcggtgcc ccagattatt ggtccccagc
      901 agcacgatga tgacccgcgg agaaatgccg tccagctccc cgttttggac acggtaatag
      961 gcattatcca cataatcaaa tccaaagccc atattcgtcg ctgcatgcgg accgaataat
     1021 ttctcccagg aacccgatcc cgtatttttt ccgtccccgg aaggctcccc tccccaatgg
     1081 tgggtaatgc tgtcccctat catcacgtat tcaggcttga cggcccggtt tcttttttta
     1141 acggcttcat gccgggcatt ccaatcatac gggtcccgct gatggggctc cggcttcagg
     1201 gtaacgggca acgcggaggc cggaatggaa ggcgcagaaa acgccagact ggaactcccc
     1261 agcaatatgg cgagcatgta ggagaggaaa ttcataatca aaaagtaaaa ccggttttca
     1321 gtttaagcaa attctgccgt gcatcaagat cgcaggcgtc acttccgccg gcagagtaaa
     1381 aaaagaccac tgcccgtccg gcaaatcctg ccgcaaccac ggccaggcga gcaccgtgtc
     1441 cggcctcttc caaggcgcgc tccatttcat ccatcccccg gcctctcccg gaacagaaac
     1501 gccctccccc gttttcttct accggggagc gggcagcttg ccgtacttgt cgtccatttt
     1561 cttcagcact acgctcaatg actttcccag tatgtcgtac cccctggcac tgggatgcag
     1621 cccatcctcc agtagcccgg gttttgggga cactccgtcc tcccctagga attcctcgcc
     1681 gggattggcg aaaaacacgc tgtttccgtc agccaggccg gatatcattt tattggtttc
     1741 cctaatcagg ggagccagct ttttttcctc cctggggaga atgcccagca acaggatttt
     1801 ggaagagggg cacttccggc gcgccaggct cacaaaagcc ttgagattgt ctgcgcaggc
     1861 ccggggagca tccttccgcc cgccaagatt attggttccc agcagcacga tgacgacccg
     1921 cggagaaatg ccgtccagct ctcccagctg caagcggtaa taggcattat ccacgtaatc
     1981 agaaccaaac cccatattgg ttaccgcatg ggaagaaaac aggctatccc aggcggtttt
     2041 tcctgttccg agagccctgt tgtccccgga aggctcgccg ccccagcggt gggtaatgct
     2101 gtcaccaaag acgacatatt ccggtttgac ggtccggttc cgctccatca ctgcttcatg
     2161 acgttttttc cagtcataca cggcccgcgg gttcacctgg ggcttcagcg tgggcggcaa
     2221 ttcatcagca caggcaccgg agaaacaagc aacgcaaaaa actcccgcca gcaataaaca
     2281 aaggcatgca taagatttca taacggtaag cgttgagata ctgcaataga gattaggaaa
     2341 gtaaagagaa ataatcttcc gtcacttttc cgcaatcgca tgttcccctc attttccgct
     2401 ccgcaacgga cagacaggtg aaacatgcca aagccagcgc acccgcgttc tcttcgcaaa
     2461 gaattagcct tgttgtcctc cggaattcac gatataatta cgcggcgcgg agttttgccg
     2521 tttccagcct ttcagaggct gcggaacgtt tttctccagt cttcttcact acattctatt
     2581 tatgatcagc atagtaaaag acttgaagga gtccaccgcc cttccgcagg aatggcaggg
     2641 attcaagccg ggagcatgga cggaatccat tgacgtacgg gatttcatcc agcataacta
     2701 cacgccctat tccggcaatg aagagtttct ttccggcccc tcccagcgca cgcttcgttt
     2761 atgggacgag ctgaaagtcc tgctgaaacg tgaaatagac aacggcggcg tgctggatgc
     2821 tgatgaagaa gtggtatcct ccatcacatc ccacaagccc ggctacattg acaaggaact
     2881 ggaagtagtc gtaggcctcc agacggacgc ccccctgaaa cgggccctga tgccctttgg
     2941 tggcttgcgc atggcccaac aggccctgga atcctacggg ttcaagatgt gcgagaaaac
     3001 cgcggacatt ttcaaaaaga tacgcaagac gcacaatgaa ggcgttttcg acgcctacac
     3061 ttccgatatc cgggcggccc gttccgccgg gattattacc ggccttccgg acgcctatgg
     3121 ccgcggccgc atcatcgggg actaccgccg ggtggccctg tacggcacgg ataagctgat
     3181 tgccgaacgc cgcaaggacc tcaaaaaccg tgaacattcc ccccttacgg atgacctgat
     3241 ccgtctgcgc gaagaaatga gcgaacaaat acgcgccctg gaagaactcg cccagctggg
     3301 cgcctcctac ggctgcgacc tcactcgccc cgccgcaaac gcccgcgaag ccgtgcaatg
     3361 gacgtacctg ggctacctgg ctgccgtgaa agaacagaat ggagcggcca tgtccctggg
     3421 ccgcgtttcc actttcttcg acatttattt cacccgcgac ctggaacagg ggctcatcac
     3481 ggaagaggaa gttcaggaaa tcatcgacca gttcgtgatg aagctgcgca tcgtccgttt
     3541 catccgcacg cctgattaca ataacctgtt ctccggagac cccacctggg tgacggaatc
     3601 catcggcggc atgggagagg acgggcgaac cctcgtcacc cgcagttcct tccgcatgct
     3661 gcagaccctg tacaacctgg ggccggctcc ggaaccgaac ctgaccgttc tgtggtcccg
     3721 gaacctgccg gaagccttca aaagcttctg cgccaaggtt tccattgaaa cgtcctccgt
     3781 ccaatacgag aacgacgacc tgatgcgccc gcattggggc gacgactacg gcatcgcatg
     3841 ctgcgtgtcc gccatgcgca tcggcaagca gatgcagttc ttcggagcgc gcgccaacct
     3901 cgccaagtgc cttctctacg ccctgaacgg cggcgtggat gaactcaagg gcaagcaggt
     3961 ggccccgccc tccccccgct acacggagga aattctcaac tatgatgagg tgatggccct
     4021 gtacgacaag atgcaggact ggctcgccaa aacttacatt gacgcgctga acatcatcca
     4081 ctacatgcac gacaaatact gctatgaacg cattgaaatg gcgctgcatg atccggaaat
     4141 tctgcgcacg atggctacgg gaatcgccgg gctttccgtg gcggcggact ccctgtccgc
     4201 catcaagtat gccaccgtaa aagccatccg caatgaagaa gggctgattg tggacttcaa
     4261 gacggaaggg gaattcccct gttacgggaa caatgacccg cgcgtggacg acatagcatg
     4321 cagcctggtc agcaatttca tggaaaaact gcgcaggctc cacacttacc gcaattccct
     4381 gcccacccag tccatcctga cgatcacctc caacgtggtg tacggcaaaa agacgggcaa
     4441 cacgccggac ggccgccgag ccggggaacc gttcgccccc ggagccaacc ccatgcacgg
     4501 aagggacagg aacggagccg tggcctccat gctctccgta gccaagctgt cctatgacga
     4561 ctccctggac ggcatctcct acaccttctc catcgttcct caggccctgg gcaaggagga
     4621 acgtgaacgc cgcgtcaagc tcgtctccct gctggacgcc tactttgccg ctacaggtca
     4681 ccacattaat gtgaacgtac tggaacggga aacgcttctc gacgccatgg atcacccgga
     4741 aaaatacccg cagcttacca tccgcgtttc cggctatgcc gtgaatttca tcaagctgac
     4801 ccgggaacag cagcaggagg tcatcaaccg cactttccac acccgttaac ccggactgca
     4861 agcggatgcc gcagtttttt ccacaaaacc atgctccgga cggagaaagc tccgtcaccg
     4921 gtttggttca ttccgtggaa tcctgcggca ccgtggacgg ccccggcatc cgcttcgtcc
     4981 ttttcctgtc cggctgcagc ctgcgctgcc gttactgcca taatccggat acctcctacg
     5041 tgcggagggg ccggctcagg agcgcggatg atgtcctcaa ggaaatagcc cgttaccggg
     5101 attttttgca ggcggcgggc ggcggcgtca ccctctccgg cggggatccc ctcttccagc
     5161 cggattttac cggtgccgtg ctgaaaggct gccggaaact gggcctgcac acctgcctgg
     5221 acacctccgg ccatctgagc gtgaatgcgg gcgaagaaat gctggcggat acggacctgg
     5281 tcctgctgga tatcaaggca tggaatccga aacggtatcg cgccctgaca ggcggggaac
     5341 tgcgccctac actggagttt gcggaacgcc tggcccccct ccgcaagccc gtgtggctga
     5401 ggtatgtgct ggtacctggc gttacggaca atctggaaga aattgaggaa ctctcccgcc
     5461 atgcggcgct tctgggcaac gtggaacgcg tggacgtgct ccctttccac cagctgggga
     5521 gattcaaatg ggatgaattg aagctggact atactttgaa ggacgtcccg gaagcgtccg
     5581 cagaactggc ggaacaggtc cgcggcattt tccggcgggg aggctttccg gcttgcgtgc
     5641 attaaccgga ttttcgcgct ttggcgccac caaccgcagc aaaccgaacg caggcatccc
     5701 cctcttccgg atgaggggcc ctcttccctc cactacagaa tccatgcaaa aaggaagacg
     5761 catttctgga aatgctccgg aatacggcat ttccgcaatt cctttcgtta ttccatcatt
     5821 ctttacggca acggcagca
//
