LOCUS       c00274_gnlPro..         3837 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|CBNGDKLJ_274.
ACCESSION   c00274_gnlPro..
VERSION     c00274_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-07 21:28:11
            Original ID  :: gnl|Prokka|CBNGDKLJ_274
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 3837
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..3837
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[280:3236]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      281..3236
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..3837
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..3837
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             3..212
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Fn3-like"
                     /gene_functions="other (smcogs) SMCOG1041:Fibronectin type
                     III-like domain (Score: 67.3; E-value: 3.2e-21)"
                     /gene_kind="other"
                     /locus_tag="ctg274_1"
                     /sec_met_domain="Fn3-like (E-value: 1.5e-22, bitscore:
                     67.3, seeds: 729, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="VTRPIKELKGFRKIHLNVGESKTVAFQITDDALGFYDNEMNYMVE
                     PGDFVFMVGGSSDDVQQITYNLKE"
     CDS             complement(281..1006)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 74.8;
                     E-value: 2.8e-23)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg274_2"
                     /sec_met_domain="Radical_SAM (E-value: 8.3e-32, bitscore:
                     98.6, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MINVHSYESMGTFDGPGLRLVVFLQGCPFRCLYCANPDTIDVKGG
                     TPTTPEEILQMAISQKAFFGKKGGITFSGGEPTLQAEALIPLFKELKANDIHICLDSNG
                     GIWNEKVEELFSLTDLVLLDIKQFNPERHRTLTGRSNEQTLRTAAWLEEHEHPFWLRYV
                     LVPGYSDSEEDTRLLGESLGKYQQIQRVEILPYHRLGVHKYEAMGWDYKLKEVKENTPE
                     QLERAEKLFKEYFTNVITN"
     CDS             complement(1008..3236)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate1"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg274_3"
                     /sec_met_domain="Gly_radical (E-value: 1.7e-47, bitscore:
                     148.2, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate1 (E-value: 0.0, bitscore:
                     1170.4, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MELNKTFTNGLWNKEINVSDFVSKNIAPYTGDASFLQGPTERTKR
                     IWDICLKALEEERNNNGVRSLDNTTVSTITSHKAGYIDKDNELIVGLQTDELLKRSIKP
                     FGGINVVAKACRENGVEVDDKVKDIFTHYRKTHNDGVFDVYTEEIRSFRSLGFLTGLPD
                     NYARGRIIGDYRRLALYGIDRLIEAKQEDLRNLTGPMTEARIRLREEVAEQIKALKDIK
                     VMGEYYGLDLSHPATSAQEAVQWVYMAYLAAIKEQDGAAMSLGNVSSFLDIFIEYDLAH
                     GKIDETFAQELIDQFVIKLRMVRHLRMQSYNDIFAGDPTWVTEAIGGRFNDGRVKVTKT
                     SFRFLQTLYNLGPSPEPNLTVLWSPELPEGFKDFCAKVSVDTSSIQYENDNLMREVRNC
                     DDYGIACCVSYQAIGKQIQFFGARANLAKALLLAINGGRCENTGTVMVKGIPVLTGETL
                     KFEEVMANYKKVLTEIARVYNEAMNIIHYMHDKYYYEKAQMAFIDTDPRINLAYGVAGL
                     SIAIDSLSAIKYAKVTARRNEIGLTEGFDTEGSFPCFGNNDDRVDHLGVDLVYYFSEEL
                     KKLPVYKNARPTLSLLTITSNVMYGKKTGATPDGRAKGVAFAPGANPMHGRDKNGAIAS
                     LSSVAKLRYRDSQDGISNTFSIVPKSLGPTPEERVENLVTMMDGYFTKGAHHLNVNVLN
                     REMLEDAMEHPEKYPQLTIRVSGYAVNFVKLSREHQLEVISRSFHERM"
ORIGIN
        1 ctgttacgcg tccgataaag gaattgaagg gcttcaggaa aattcatctg aatgtgggtg
       61 aaagcaaaac tgtagctttt cagataactg acgatgcact gggattctat gacaatgaga
      121 tgaattatat ggtagaacct ggtgatttcg tctttatggt gggtggcagt agtgatgatg
      181 tacagcagat tacctataac ttgaaagaat aggattctta ccctataaat gaaaagcaag
      241 gctgtctgga atcatatccg gacagccttg cttttctgtt ttagttggtt atcacattcg
      301 tgaaatactc tttgaacagt ttttctgccc gttccagttg ttccggagta ttttccttca
      361 cttctttcaa tttgtaatcc catcccatgg cttcgtattt gtgaacaccc agacggtgat
      421 aaggaagtat ttccacccgt tgtatctgct ggtatttgcc aagactttca cccagcaggc
      481 gggtgtcttc ttccgaatcg ctgtaaccgg ggaccagtac atagcgcagc cagaaggggt
      541 gttcatgttc ttcgagccag gcagcggtgc ggagtgtctg ttcgttgctt cgtcccgtca
      601 gcgtacggtg gcgttcggga ttgaattgct tgatgtccag cagcacgagg tcagtcagac
      661 taaagagctc ttccaccttt tcattccaga tgcctccgtt actgtccaga cagatatgga
      721 tgtcgttcgc cttcagctcc ttgaaaagag gaatcagcgc ttctgcttgc agggtaggct
      781 caccgccgga gaaagtaata cctcctttct tcccgaagaa tgccttttgg ctgatagcca
      841 tttgcagaat ctcttccgga gtggtgggag tgcctccttt tacatcaatc gtatccggat
      901 tggcacaata cagacaacgg aacggacatc cttgcaggaa aacgacgagc cggagacccg
      961 gcccgtcgaa tgttcccata ctttcgtagg agtgtacatt tatcatatta cattctttcg
     1021 tggaatgaac ggctgatgac ttccaactga tgctcgcggc tcaacttcac gaagttcacg
     1081 gcatagccgg aaacgcggat ggtgagttgc ggatactttt caggatgttc catggcatct
     1141 tccagcattt cgcggttcag tacgtttacg ttcaaatggt gggcaccttt ggtgaagtag
     1201 ccgtccatca tggttaccag gttctctaca cgttcttccg gtgtcgggcc cagtgatttg
     1261 ggaacaatgg agaacgtatt gctgatgccg tcctgcgagt cgcggtagcg caatttggct
     1321 acggagctca aggaagcgat ggcaccgttc ttgtcacgtc cgtgcatcgg gttggcaccc
     1381 ggagcaaagg caacaccttt ggcgcgtccg tccggagtgg caccggtctt cttgccgtac
     1441 atcacgttgg aggtgatagt cagcaaggaa agggtgggac gggcattctt gtagacaggc
     1501 agctttttca gttcttcgct gaaatagtaa acgaggtcta ctcccaggtg gtccacacgg
     1561 tcgtcattat tgccgaagca ggggaatgag ccttcggtgt cgaaaccttc ggtcaggccg
     1621 atttcgttgc ggcgtgcagt gactttggca tatttgatgg cggacagtga gtctatggcg
     1681 atggaaaggc cggcaacacc gtatgccaga ttgatgcgtg ggtcggtatc gatgaatgcc
     1741 atctgggctt tctcataata atacttatca tgcatgtagt ggatgatatt catggcctcg
     1801 ttgtagacac gggctatttc agtcagtacc ttcttgtagt tggccataac ttcctcaaac
     1861 ttcagtgtct cgccggtcaa tacgggaata cctttcacca tgacagttcc ggtgttttcg
     1921 caacggccac cgttgatggc aagcagcagg gccttggcaa ggttggcacg tgcgccgaag
     1981 aactggattt gcttgccgat ggcttggtag gatacacagc aggcaatgcc gtaatcgtcg
     2041 cagttgcgca cttcgcgcat caggttgtcg ttttcgtact ggatggagct ggtatctaca
     2101 gaaactttgg cgcagaaatc cttgaagcct tccggcagtt cgggactcca caatacggtc
     2161 aagttcggtt ccggagaagg accgaggttg tatagcgtct gcaaaaagcg gaaagaagtc
     2221 ttggtgacct tcacgcgtcc gtcgttgaag cgtccgccga tggcttcggt cacccaagtc
     2281 gggtcgccgg caaagatatc attgtacgac tgcatgcgca gatggcgtac catacgtagt
     2341 ttgatgacga actggtctat cagttcctgt gcaaacgtct cgtcaatctt gccgtgagcc
     2401 aaatcgtatt cgatgaaaat gtcgaggaac gaagatacgt tacccagtga catggctgct
     2461 ccgtcctgct ctttgatggc agcaagatat gccatgtata cccattgcac ggcctcttga
     2521 gctgatgtag ccggatggct gaggtcgagt ccgtaatatt cgcccataac cttgatgtcc
     2581 ttcaaagctt tgatttgttc tgccacttct tcgcgtagac ggatgcgtgc ttcggtcatc
     2641 gggccggtga ggttgcgcaa gtcctcctgc ttggcttcaa tcagacggtc gatgccgtag
     2701 agagccaatc ggcggtagtc gccgatgata cgtccgcggg catagttgtc gggcaatccg
     2761 gtaaggaatc ccagtgagcg gaaggaacgg atttcttcgg tatatacatc gaatacgccg
     2821 tcgttatgtg ttttgcggta atgggtgaag atgtctttta ctttatcgtc cacttccact
     2881 ccgttttcac ggcatgcttt tgctaccacg ttgatgcctc cgaaaggttt gatggatcgt
     2941 ttcaacagtt catcagtctg caaaccgaca ataagttcat tgtccttatc aatatacccg
     3001 gctttatgag aagtgatggt agatacggtg gtattgtcca gtgaacggac gccattgttg
     3061 ttcctttctt cttcgagagc cttcagacag atgtcccaga tacgtttggt acgttcggta
     3121 ggtccctgca agaaagatgc atccccggta tagggtgcga tgtttttact gacgaaatca
     3181 ctgacattga tttctttatt ccaaagccca ttggtaaagg ttttgttcaa ttccataaaa
     3241 tttgtttttg ttagtaggtt agataatgtt atcctttaga gcgtgcaaaa atactgaata
     3301 aattccgtaa ttttataaga gatacttctc tttttatgtt gaacagcata aaataactat
     3361 tattaggtag tgaatgtccg ggggagtaga gatgttaata ctttctttat atgtggttta
     3421 tgaattgaaa caagaaccga ataataatct gcattattta caaatgatgt aggtttaagg
     3481 aacagtcgta ttctgaacct aattcagtcg tatagtgaat gccattcagt cgtatactga
     3541 atagggttca atcgtatact gaatgtggct ctttgtaaat atactgatag ccagtaagtt
     3601 ataaaactct gtcttgccct tgataaattt caacaaatag cctaaatgcc gttatgttcg
     3661 ttccgtagtg ggtataaaca agaattttgg tataattgct ctgtttttgt gcagaaaagt
     3721 ttgtagaata aaaaatgatt tgtatctttg caccgcttta aaagaaaagc acttctgaaa
     3781 aagaagtttt ggagaggtgg cagagtggtc gattgcggcg gtcttgaaaa ccgttgt
//
