LOCUS       c00320_gnlPro..         2831 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|CBNGDKLJ_320.
ACCESSION   c00320_gnlPro..
VERSION     c00320_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-07 21:28:11
            Original ID  :: gnl|Prokka|CBNGDKLJ_320
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 2831
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..2831
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[1:2829]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      2..2829
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..2831
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..2831
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             2..1495
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate2"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg320_1"
                     /sec_met_domain="Gly_radical (E-value: 2.4e-15, bitscore:
                     45.2, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate2 (E-value: 1e-205, bitscore:
                     672.7, seeds: 33, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="IDRLIEDKQDQKDSTGRVMTDDVIRLREELSEQMVALKMMKEMAA
                     SYGCDISKPATNVQEAIQATYFGYLCSVKEQNGAAMSLGRTSTFIDCYAERDLANGTFT
                     EEQIQEFVDHFIMKLRCIKFARTPEYNQIFAGDPVWVTESLGGVGVDGRPMVTKTSFRY
                     LHTLTNLGPSPEPNLTVLWSTRLPENWKRYCAKMSIQTSSIQYENDDLMRVTHGDDYGI
                     ACCVSSMVIGKEMQFFGARANLAKCLLYALNGGVDEVTKKQVGPKYRAVTGDVLDYDDV
                     YAKFMDMMEWQADVYVNTLNIIHYMHDKYCYERAEMALHDRDVKRYFATGVAGLSIVAD
                     SLSAIKYAKVEVVRDEDGVIVDFKTTGEFPKYGNDDDRVDTIAQEIVHKFMTAIRRHHT
                     YRNGIPTTSILTITSNVTYGKATGNTPDGRKKGQPFAPGANPMHGRDTHGAVASLSSVS
                     KLPFKDAQDGISNTFTIIPGALGKEDQVFAGDIELDLNN"
     CDS             1659..1862
                     /locus_tag="ctg320_2"
                     /transl_table=11
                     /translation="MDDKKMVDDLVKMLDAGMMQGVGHVNVDTDSSMEESKNVQTMGCT
                     DCSRTPLACSVPTLEQGLDDYQ"
     CDS             1943..2185
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg320_3"
                     /sec_met_domain="Gly_radical (E-value: 4.3e-29, bitscore:
                     89.4, seeds: 99, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MATNNAQVDNLVNLLDGYATKGGHHLNVNVLNRDTLLDAQKHPEN
                     YPQLTIRVSGYAVNFIKLTPEQQADVISRTFHEAI"
     CDS             2359..2829
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 80.4;
                     E-value: 5.1e-25)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg320_4"
                     /sec_met_domain="Radical_SAM (E-value: 1.2e-29, bitscore:
                     91.9, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MQGRIHSLESFGTVDGPGTRFVVFVQGCPMRCAYCHNPDTWPMDG
                     GTLMEPEEIFEQYRRNAAFYTKGGITVTGGEPLMQVDFLIDLFTIAKKEGVHTCIDSSG
                     IAFKPQNTEWIKKLDHLMTLTDLVMLDIKHIDPEKHKELTAQPNDGILAFAAY"
ORIGIN
        1 tatcgacagg ctgatcgagg acaagcagga tcagaaggat tctaccggcc gtgttatgac
       61 tgatgatgta atccgtctcc gtgaagaact ttccgagcag atggttgccc ttaagatgat
      121 gaaggaaatg gccgcttctt acggatgcga tatttccaaa cctgcaacca acgtacagga
      181 agccattcag gctacctact tcggatatct ttgctccgta aaagagcaga acggcgcggc
      241 tatgtcactt ggacgtactt ctactttcat tgactgctac gcagaaagag atttggcgaa
      301 cggaaccttc acggaagaac agattcagga attcgttgac cacttcatca tgaaattaag
      361 atgcattaaa tttgcacgta ctcctgaata caaccagatt ttcgcaggcg acccggtttg
      421 ggtaacagaa tccctcggcg gtgtcggcgt ggacggacgc ccgatggtaa caaagacttc
      481 tttccgttac ctgcacacac tgaccaacct tggcccttct ccggaaccga acctgacagt
      541 actctggtcc acaaggcttc ctgaaaactg gaagagatat tgtgcgaaga tgtccatcca
      601 gacttcttcc atccagtacg aaaacgacga ccttatgaga gtgacacacg gcgacgacta
      661 cggtatcgca tgctgcgtat cctccatggt aatcggtaaa gaaatgcagt tcttcggagc
      721 tcgtgctaac cttgcaaaat gtctgctcta cgctttgaac ggcggtgtgg acgaagtaac
      781 aaagaaacag gttgggccga aataccgtgc cgtaaccggt gatgttcttg attatgacga
      841 tgtttacgct aaattcatgg atatgatgga atggcaggct gacgtatatg tcaatacctt
      901 gaacatcatc cactatatgc atgacaaata ttgctatgag agagccgaga tggcgcttca
      961 cgacagagat gttaagagat acttcgcaac gggtgttgca ggcctttcca tcgtagccga
     1021 ctctttatcc gcaatcaaat atgctaaagt tgaagttgta agggacgaag acggcgtaat
     1081 cgttgacttc aagacaaccg gcgaattccc gaaatacggt aacgatgatg accgcgtgga
     1141 tacgattgca caggaaatcg tacacaaatt catgactgcc atcagaaggc accacaccta
     1201 cagaaacggt attcctacca cttccatcct taccattact tccaatgtaa cctatggtaa
     1261 ggctaccgga aacacacctg acggacgtaa gaaaggccag cctttcgcac ccggcgctaa
     1321 cccgatgcac ggccgtgata ctcacggtgc agtagcttcc ctgtcttccg tatccaagct
     1381 tcctttcaaa gatgcacagg acggtatctc caataccttc accatcattc ctggcgcact
     1441 tggcaaagag gatcaggtat ttgcaggtga cattgaactt gatttaaata actaattgtg
     1501 acattgcatt taaaagtcaa tcccccgctg caggcagagg gggattggcc ccgcggcaag
     1561 cagatgagga ttgcctccgc ggcagtcacc aaatatccgc acaaatgcgg ctgcttggct
     1621 cattgtctgc gggaataaaa tgaaaacgag ggtttcatat ggatgacaaa aaaatggttg
     1681 atgatctcgt taagatgttg gatgccggca tgatgcaggg tgtaggtcat gtgaacgtgg
     1741 atacggacag ttccatggaa gaatccaaga atgttcagac aatgggttgc acagactgct
     1801 ccagaacccc gttggcatgc agtgttccca ctttggagca gggcttggat gactatcagt
     1861 gaccgcattt tataagcggc attgatgtaa gacatgatat ctgttcatca tgaaccgtct
     1921 atgaataaag gaggaaacta aaatggcaac aaataacgca caggttgata accttgtaaa
     1981 tttattggat ggatatgcaa caaaaggcgg ccatcacctc aatgtgaacg ttttgaacag
     2041 ggataccctt ctggatgctc agaaacatcc tgagaattat cctcagctga ccatccgtgt
     2101 atccggatat gccgttaact tcattaagct gacaccggaa cagcaggcag acgttatttc
     2161 ccgtactttc catgaggcaa tctaaagcct tacacgacgg aagagttttc gggtgaggaa
     2221 aagaaccttg ggattttccc aaggttcttt ttgaaattaa agcacccgtt ccttaagcag
     2281 tctttaccct cgcggcagtt gcttttttcc tccattctgc tatactataa aaagaatatc
     2341 cgaaaggaga ttatcattat gcaaggaaga atccactcat tagaatcttt tggcacagtt
     2401 gacggtccag gtacccgttt tgtcgttttt gtgcaaggct gtcccatgcg ctgcgcttac
     2461 tgccacaacc ccgatacatg gcccatggac ggcggcactc ttatggaacc ggaagaaata
     2521 tttgagcagt acagaagaaa cgcagcattc tatacaaaag gcggcataac cgtaacggga
     2581 ggagagcctc tgatgcaggt agattttctc atcgacctgt ttaccattgc caaaaaagag
     2641 ggcgtacaca cctgcattga cagttcgggc atagctttca agccccaaaa tacggaatgg
     2701 atcaagaaac tggaccactt aatgacgctg accgacctcg tcatgctgga catcaaacat
     2761 attgaccctg agaaacacaa agaactcact gcccagccta acgacggcat ccttgccttt
     2821 gccgcatatt t
//
