LOCUS       c00258_gnlPro..         4836 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|KOCJGHKI_258.
ACCESSION   c00258_gnlPro..
VERSION     c00258_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-07 16:08:31
            Original ID  :: gnl|Prokka|KOCJGHKI_258
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 4836
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..4836
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[805:3785]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      806..3785
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..4836
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..4836
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             806..3046
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate2"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg258_1"
                     /sec_met_domain="Gly_radical (E-value: 8.1e-46, bitscore:
                     142.8, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate2 (E-value: 2.5e-285, bitscore:
                     935.1, seeds: 33, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKFENENWAQFKGEIWKREINVRDFIQNNYKPYEGDDSFLKDSSD
                     KTKKVWDKLTEMFKVEREKGVYDAETKYPQGIDTYGPGYIDKENEVIVGLQTDAPLKRG
                     IYPKCGTRMVENSLEAYGYKLDPMTKEIFTKYRKTHNEGVFSAYTDEMLAARRSAIITG
                     LPDAYGRGRIIGDYRRVALYGTEILIAEKKHYLKRLDIQELTEEIIQSREEISEQIKAL
                     KAFEKMCAGYGFDVTKPAKDAREAVQFVYLGYLAAVKDQDGAAMSLGRTSTFLDIYIEK
                     DIREGKLTEEEAQELIDQFIIKLRIVRFLRTPEYNDLFSGDPVWVTESLGGQGVDGRSL
                     VTKTSYRYLHTLYNLGPAPEPNLTVLWFNNAPENWKRFCAKVSIDTSAIQYENDDLMRP
                     DYGDDYGIACCVSPMKIGKQMQYFGARANLAKCLLYAINGGRDERSGVQVAPRFEPITS
                     EYLDYNEVMEKYEQMMRWLAKVYVNALKIIHYMHDKYAYEAFEMALHDGSVERIRATGI
                     AGLSIVADSLAAIRDTKVKVIRDERGLAVGFEREGDYVPFGNNDDRTDSIAVDITEKFM
                     EYLRQHQTYRNATPTQSILTITSNVVYGKKTGATPDGREGGTPFAPGANPMNGRDTKGA
                     VAALASVAKLPFQHAHDGISYTFAVSPATLGKERDIQIANMVGLLDGYFTPDGGQHLNV
                     NVFDKELLLDAMEHPENYPQLTIRVSGYAVNFVKLTREQQLDVISRTINHSL"
     CDS             3054..3785
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 72.3;
                     E-value: 1.6e-22)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg258_2"
                     /sec_met_domain="Radical_SAM (E-value: 6.2e-37, bitscore:
                     115.3, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="LEIKGNIHSLESFGTVDGPGIRFVVFMQGCPLRCLYCHNPDTWDS
                     KGLAKYQMTPEELLAEVLRYKNFIAKGGVTVTGGEPLLQAEFLREFFRLCREAGIHTAL
                     DTSGYICTPKALAVLEYADLVLLDIKTIDPDLHPRLTAVKLDNTLRFLDELEKRGVPVW
                     IRHVIVPGLTDNDEGLDKLAEYISHYTVVQKAELLPYHTMGAYKYEAQGLDYKLKEVEP
                     LSAERLANAKEIFKKHGITIW"
     CDS             3871..4773
                     /locus_tag="ctg258_3"
                     /transl_table=11
                     /translation="MEQHHHDHHHHEITSLNKAFIIGISLNLAFVVAEFTAGFWYNSLG
                     LLSDAGHNLGDVASLVLAMLAFRLAKVHPTCSYTYGYKKSTVLVSLLNAVILLVAVGII
                     IAESIGKLLHPRPVEGDAIAWVAGIGVVINALTAWLFMKDKDKDLNVKGAYLHMAADAL
                     VSVGVVVSGIVIAHTGWYIIDPIIGLLIAIVIIVSTWGLLRDSIRLSLDGVPAGTDIDK
                     VEKTIHSVEGVEGVHHLHIWAISTTETALTAHVSVADISEMERIKQEIKHDLQHVGIQH
                     ATLEFETSGFKCDGHCCSD"
ORIGIN
        1 tgttgcgggg gaaaatctga aggtagaata attatatagg taatattttc cctgtaatcc
       61 ctatttatgg ttagaaatga gctcgtttta catatatata ggtattgcct gtgcctttcc
      121 gtggttttac ctttgccgta tcaattaagt tttactatta gtataaatcg tcctgaccaa
      181 aatccgattt gttgattttt atgtacacct attatttaag gtaaataccc atggaaggga
      241 gtttttttat tggttgtccc tttcatatat aaaagggagc catcctgtga agaatggctc
      301 ttttttattt atcaaagaaa gcaatttttg caaatattgt ttattccaat gatcaatttt
      361 cccgattata acacagaata ggtagtagga agctcttttt ttactatata ataggtattg
      421 tgtactcccc ttgacggctc tatctttgca acatagaaaa aagtaacact attagtataa
      481 tcgctatatc aaaacctttt gctaattatg taagcatata agatgattaa acgtgtgtcg
      541 gaaatttttt attggttgtt tccggcataa aaaagggact ctcctgcgaa ggatggttcc
      601 ttttttattt ttttgatatt atttataatc cttcctgttt gatacctata tcaggcgata
      661 cgctgcacta tatctaccgt tttagtaact atttgtaggg attttggccg ttgggaatac
      721 atgatgtttt tgttgattgt ttcgcatgcc atataaataa cgtacctttg caccgaaatt
      781 aaaaccgaag aaaacaaaat aaggtatgaa gtttgaaaac gagaactggg cgcagttcaa
      841 aggcgaaatt tggaaaagag agattaacgt aagggatttt atccagaaca actacaaacc
      901 ttacgaaggg gacgactctt tcttgaaaga ctcctcagat aagacgaaga aagtttggga
      961 taaattgacc gaaatgttta aggttgaaag ggagaagggt gtttacgacg ctgaaacaaa
     1021 atatcctcaa ggtatagata cttacggacc aggttatatt gacaaagaaa acgaagtaat
     1081 tgtcggcctg caaaccgatg cccccctgaa acgcggtatt tacccgaaat gtggtacccg
     1141 catggtggaa aacagtctgg aagcatatgg ctacaaactg gacccgatga caaaagaaat
     1201 cttcacgaaa tacagaaaga cgcataacga aggcgtattc tccgcttata cagatgaaat
     1261 gcttgcagcg cgccgttccg ctatcatcac cggcctgccg gatgcttacg gacgcggacg
     1321 tattatcggt gactaccgcc gtgtagcact ttacggtacg gaaatcctga tcgctgaaaa
     1381 gaaacattat ctgaaaagac tcgatattca ggaactgacc gaagagatca tccagagccg
     1441 tgaggaaata tccgaacaga tcaaggcgct gaaggctttc gaaaagatgt gtgccggtta
     1501 tggtttcgac gtgaccaagc ctgcaaagga tgcacgcgaa gcggttcagt ttgtctatct
     1561 gggttatctg gctgcggtga aggaccagga tggggctgcc atgtcgttgg gacgtacttc
     1621 tactttcctc gatatttata ttgaaaaaga tatccgcgaa ggcaagctga cggaagaaga
     1681 agctcaggag ctgatcgacc agtttattat caaactgcgt atcgtccgtt tccttcgtac
     1741 tccggaatat aacgacctgt tctccggtga ccctgtttgg gtaaccgagt ctttgggcgg
     1801 acagggtgtc gacggccgtt cattggtgac gaagacatct taccgttatc tgcatacatt
     1861 atataacctc ggcccggcac cggaacccaa cttaacggta ctgtggttca acaacgctcc
     1921 tgaaaactgg aaacgtttct gtgcgaaggt ttctatcgat acttccgcta tccagtatga
     1981 gaacgacgat ctgatgcgtc cggattatgg agatgattat ggtattgcct gctgcgtgtc
     2041 tccgatgaag atcggtaagc agatgcagta tttcggcgca cgtgccaacc tggctaaatg
     2101 tttgctgtat gccatcaacg gcgggcgcga tgaaagatca ggtgtacagg tggctccgag
     2161 gtttgaaccg atcaccagcg aatacctgga ttacaacgaa gtgatggaga aatacgaaca
     2221 gatgatgcgc tggctggcta aagtttatgt gaacgcactg aagatcatcc attacatgca
     2281 cgataaatat gcgtatgaag cattcgagat ggctttgcat gacggttcgg tagaacgtat
     2341 ccgcgcaaca ggtattgccg gcctttctat cgttgccgac tcattggctg ctatccgcga
     2401 tacgaaagta aaagtgatcc gcgacgaacg tggcctggct gtcggtttcg aacgtgaagg
     2461 cgattatgtt cctttcggta acaatgacga tcgtacagat agtattgctg tcgacatcac
     2521 tgaaaagttc atggaatatc tgcgtcagca ccagacatac cgtaatgcaa ctccgacaca
     2581 gtctatcctg actattacgt ctaatgtcgt atacggaaag aagacaggtg caactcccga
     2641 cggtcgcgaa ggtggtacgc cgtttgctcc gggtgcgaac ccgatgaacg gacgtgatac
     2701 caaaggcgct gttgctgcac tggcttctgt tgctaaactg ccgttccagc atgcacacga
     2761 cggtatctct tatacattcg ctgtttctcc tgcaacgtta ggaaaagaac gtgatattca
     2821 gatcgccaat atggtcggcc tgctggacgg ttactttact cctgacggcg gacagcatct
     2881 gaatgtgaac gtattcgaca aggagctgtt gctcgatgct atggagcatc cggaaaacta
     2941 tccgcagttg acaatccgtg tatcgggtta tgcagtaaac tttgtgaaac tgacacgcga
     3001 acagcagctg gatgtgattt caagaacaat caatcattct ttgtaattga aagttggaaa
     3061 ttaaaggaaa catacattca ctagagagtt tcgggaccgt tgatggtccc ggaattcgtt
     3121 ttgttgtctt tatgcagggg tgtccgctcc gttgcctgta ttgccacaat ccggatacct
     3181 gggatagcaa agggcttgcc aaatatcaga tgacgcctga agaattattg gctgaggttt
     3241 tacgatataa gaactttatc gccaagggtg gcgtgactgt caccggggga gaacccttat
     3301 tgcaagctga atttctcagg gagtttttcc gtctttgccg ggaagccggt attcatacgg
     3361 cgctcgatac atccggctat atctgcacac cgaaagccct ggcggttctg gaatatgccg
     3421 acctggtctt attggatata aaaaccatcg atcccgacct gcatccccgt ctaacggccg
     3481 tgaaactgga taatacgtta cgtttcctgg acgaactgga aaaacgcggt gtcccggttt
     3541 ggatacgcca tgtcattgtg cccggactga cggataatga cgaaggcttg gataaactgg
     3601 ccgaatacat cagccactat acggttgtac agaaggcgga actcttgcct tatcacacga
     3661 tgggcgctta taaatacgag gcacaaggtc tcgactacaa actgaaagag gtagaacctt
     3721 tatcggccga acgtctggca aatgccaaag aaatatttaa gaagcacggt ataaccatct
     3781 ggtaatttac ctataatcag gtatattttt attgctctaa aaaacatagt tttgtcccat
     3841 attgttactc aaataaaagt aggaatgaat atggaacaac atcatcacga tcatcaccac
     3901 cacgaaataa cctctttgaa caaggctttc attataggta tttccctgaa cctggctttt
     3961 gtcgtagccg aatttacggc tggcttctgg tataattcat taggactttt gtcggacgca
     4021 gggcataacc tgggagatgt agccagcctg gtactggcta tgctggcttt ccggctggct
     4081 aaggtgcacc cgacttgcag ctatacctat ggatataaga aaagcactgt actcgtatcc
     4141 cttctgaatg cggtcatcct cttggtggcc gtcgggatta ttattgctga aagtatagga
     4201 aaactgctgc atccgcgtcc ggtcgagggt gatgcgatcg cttgggtggc aggtatcgga
     4261 gtcgttataa atgcacttac ggcatggctt tttatgaagg ataaggataa agacttgaat
     4321 gtgaaaggtg cttacttaca tatggctgcc gatgcactgg tatcggtcgg cgtggttgtc
     4381 tccggaattg ttattgccca taccggatgg tatatcatcg acccgattat cggcctgttg
     4441 attgcgatcg ttatcattgt ttctacctgg ggactgctgc gcgacagtat ccgtctctct
     4501 ctggacggtg tgccggcggg aacggacatc gataaggtgg aaaagacgat acattcggtt
     4561 gaaggcgtcg aaggtgtcca ccatcttcat atctgggcga tcagtacgac cgaaacggca
     4621 cttaccgccc atgtttccgt tgcggatatt tcagaaatgg aacgtatcaa acaggagata
     4681 aaacacgacc ttcagcatgt gggaatccag catgctacgc tcgaatttga aacgtcggga
     4741 ttcaagtgcg atgggcactg ttgctcagac tgaaacaggt atattataaa cctgatttac
     4801 tgatactcaa agattttgtc tatgactgca taaaac
//
