LOCUS       c00658_gnlPro..         2355 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|LJBHKMFB_658.
ACCESSION   c00658_gnlPro..
VERSION     c00658_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-07 21:22:51
            Original ID  :: gnl|Prokka|LJBHKMFB_658
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 2355
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..2355
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[2:2273]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      3..2273
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..2355
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..2355
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             3..1538
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate2"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg658_1"
                     /sec_met_domain="Gly_radical (E-value: 4.3e-47, bitscore:
                     146.3, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate2 (E-value: 1e-178, bitscore:
                     582.5, seeds: 33, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="HYPLRRQRQMCIRDRYLAYLAAVKDQDGAAMSIGRTSTFLDIYIE
                     KDIREGKLTEEEAQELVDQLIIKLRIVRFLRTPEYNDLFSGDPVWVTESLGGQGVDGRS
                     LVTRTSYRYLHTLYNLGPAPEPNLTVLWFKNAPENWKRFCAKVSIDTSAIQYENDDLMR
                     PDYGDDYGIACCVSPMKIGKQMQFFGARANLAKCLLYAINGGRDERSGVQVAPMFEPVR
                     GEYLEYDEVMAKYEQMMRWLAKVYVNALKIIHYMHDKYAYEAFEMSLHDGDVERIRATG
                     IAGLSIVADSLAAIRDTKVRVIRDERGLAVDFEREGEYVPFGNNDDRTDSIAVEITEKF
                     MEYLRQHETYRHAKPTQSILTITSNVVYGKKTGTTPDGRIGGTPFAPGANPMNGRDTKG
                     AIAALASVAKLPFQHAHDGISYTFAVSPATLGKEREVQINNLVSLLDGYFTPDGGQHLN
                     VNVFDKDLLIDAMEHPEKYPQLTIRVSGYAVNFVKLTREQQLDVISRTINHSL"
     CDS             1539..2273
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 67.6;
                     E-value: 4.7e-21)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg658_2"
                     /sec_met_domain="Radical_SAM (E-value: 2.6e-34, bitscore:
                     106.2, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="VIKGKIHSLESFGTVDGPGIRFVVFMQGCPLRCLYCHNPDTWNPK
                     GKVKYQMTPGELLTEVLRYKSFIARGGVTVTGGEPLLQPEFLKEFFRLCQEQGLHTALD
                     TSGFVCTSKAWEVLDYADLVLLDIKTLNPDLHPLLAGVKQDNTLLFLDELERRGIDTWI
                     RHVIVPGYTDNDEWLEALARYVSSYKVVRKVELLPYHTMGTYKYEQLGLDYPLKGVEPL
                     SKERLDNAKAIFSRYKPENNKA"
ORIGIN
        1 tacactatcc tcttcgtcgg cagcgtcaga tgtgtataag agacaggtat ttagcttatc
       61 tggcggcggt aaaagaccaa gatggcgcgg cgatgtctat cggacgtact tctacgtttt
      121 tggatattta tatcgagaaa gacatccgtg agggtaagtt gaccgaagag gaggcgcaag
      181 aattggtaga ccagctgatc atcaagttgc gtatcgttcg tttcttacgt acgccggaat
      241 acaatgattt gttctccggt gatccggttt gggtaacaga gtccttgggc ggccaaggag
      301 tggatggccg ttctttggta acaagaactt cctatcgtta tctccataca ttatataatc
      361 taggtccggc accagaaccc aatttgacgg ttctgtggtt taagaacgct cccgagaact
      421 ggaaacgttt ctgtgccaag gtctctatcg atacgtcggc tatccaatat gagaacgacg
      481 atttgatgcg tccggattat ggagatgatt atggtatcgc ttgttgcgtt tctcctatga
      541 agatcggtaa gcagatgcag ttcttcggag cccgtgcgaa tttggcgaaa tgtttattat
      601 atgcgatcaa cggaggacgg gatgagcgca gcggcgtaca ggtagccccg atgttcgagc
      661 cggttcgcgg cgagtatctg gaatacgatg aggtgatggc taagtatgag caaatgatgc
      721 gctggttggc gaaagtgtat gtgaacgcct tgaagataat ccattatatg catgataaat
      781 acgcgtatga ggcattcgag atgtcgcttc acgatgggga tgtagagcgt atccgtgcga
      841 caggtatcgc cggtctttct atcgttgccg attcgttggc tgctatccgt gatacgaaag
      901 tacgtgtgat ccgtgacgag cgtggcttgg cggttgattt cgaacgtgag ggcgaatatg
      961 ttcctttcgg taataacgat gatcgtacgg atagtatcgc tgtagagatc accgagaagt
     1021 tcatggaata tttgcgtcag cacgagacct atcgccatgc gaaacctacg cagtccatcc
     1081 ttacgatcac ctctaatgtg gtatatggta agaagactgg tacgacaccg gatggccgta
     1141 tcggtggaac tcctttcgct ccgggtgcga accctatgaa cggtcgtgat acgaaaggtg
     1201 ctatcgctgc gctagcttcg gtagcgaagc ttccattcca gcatgcgcat gacggcatct
     1261 cttatacttt cgctgtttct ccggctacgt tgggaaagga gcgtgaagtg cagatcaata
     1321 atctggttag cttattggat ggctatttta ctccggatgg aggtcagcat ctgaatgtta
     1381 acgtatttga taaggatttg ttgatcgacg ctatggagca tcccgagaaa tatccgcaac
     1441 ttacgatccg tgtatccgga tatgcggtaa acttcgtgaa actgactcgt gaacaacagt
     1501 tggatgttat ttcaagaacc attaaccatt cactgtaagt gataaaaggt aagatacatt
     1561 cattagagag tttcgggacc gtagatggtc ccggaattcg ctttgtcgtt tttatgcagg
     1621 gctgcccgtt gcgttgcctg tattgccata acccggatac ttggaacccg aaagggaagg
     1681 tgaaatatca gatgactccc ggcgagctat tgacggaagt cttacggtat aagagtttta
     1741 tcgcccgtgg gggggtgact gttaccggtg gggaaccttt attgcaacct gagtttctga
     1801 aagagttttt ccgtttgtgc caagagcaag gtcttcacac agctttggac acatctggtt
     1861 tcgtatgtac ctcaaaagct tgggaagtct tggattatgc ggacttagtg ttattggaca
     1921 tcaaaacctt aaacccggat ttacatccgt tactggcggg tgtcaagcag gataatacgc
     1981 ttctgttctt ggatgaattg gagagacgtg gcatagatac ttggattcgc catgttattg
     2041 tccccggata tacggataat gacgagtggc tggaggcgtt agctcggtat gtcagttcat
     2101 ataaagtggt tcgcaaagtg gaattattgc cttatcatac gatgggaacc tataaatatg
     2161 agcaattagg cctcgattat ccgttaaagg gggtggaacc cttatcaaaa gaacgtttag
     2221 ataatgcgaa agcgatattc tctcgctaca aaccagaaaa taacaaggcc tgatctctcg
     2281 atcaagcctt gcgattatgc aacagctaat cagggaaaac aatctaatcc ctgtctctta
     2341 tacacatctc cgagc
//
