LOCUS       c00328_gnlPro..         5230 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|KLHOFJPJ_328.
ACCESSION   c00328_gnlPro..
VERSION     c00328_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-05 15:37:53
            Original ID  :: gnl|Prokka|KLHOFJPJ_328
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 5230
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..5230
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[2:2481]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      3..2481
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..5230
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..5230
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             3..1742
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate2"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg328_1"
                     /sec_met_domain="Gly_radical (E-value: 9.1e-46, bitscore:
                     143.3, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate2 (E-value: 7.5e-218, bitscore:
                     713.1, seeds: 33, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="YGRGRIIGDYRRVALYGTEILIAEKKHYLKRLDIQELTEEIIQSR
                     EEISEQIKALKAFEKMCAGYGFDVTKPAKDAREAVQFVYLGYLAAVKDQDGAAMSLGRT
                     STFLDIYIEKDIREGKLTEEEAQELIDQFIIKLRIVRFLRTPEYNDLFSGDPVWVTESL
                     GGQGVDGRSLVTKTSYRYLHTLYNLGPAPEPNLTVLWFNNAPENWKRFCAKVSIDTSAI
                     QYENDDLMRPDYGDDYGIACCVSPMKIGKQMQYFGARANLAKCLLYAINGGRDERSGVQ
                     VAPRFEPITSEYLDYNEVMEKYEQMMRWLAKVYVNALKIIHYMHDKYAYEAFEMALHDG
                     SVERIRATGIAGLSIVADSLAAIRDTKVKVIRDERGLAVGFEREGDYVPFGNNDDRTDS
                     IAVDITEKFMEYLRQHQTYRNATPTQSILTITSNVVYGKKTGATPDGREGGTPFAPGAN
                     PMNGRDTKGAVAALASVAKLPFQHAHDGISYTFAVSPATLGKERDIQIANMVGLLDGYF
                     TPDGGQHLNVNVFDKELLLDAMEHPENYPQLTIRVSGYAVNFVKLTREQQLDVISRTIN
                     HSL"
     CDS             1750..2481
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 72.3;
                     E-value: 1.6e-22)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg328_2"
                     /sec_met_domain="Radical_SAM (E-value: 4.6e-37, bitscore:
                     116.4, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="LEIKGNIHSLESFGTVDGPGIRFVVFMQGCPLRCLYCHNPDTWDS
                     KGLAKYQMTPEELLAEVLRYKNFIAKGGVTVTGGEPLLQAEFLREFFRLCREAGIHTAL
                     DTSGYICTPKALAVLEYADLVLLDIKTIDPDLHPRLTAVKLDNTLRFLDELEKRGVPVW
                     IRHVIVPGLTDNDEALDKLAEYISHYTVVQKAELLPYHTMGAYKYEAQGLDYKLKEVEP
                     LSAERLANAKEIFKKHGITIW"
     CDS             2567..3469
                     /locus_tag="ctg328_3"
                     /transl_table=11
                     /translation="MEQHHHDHHHHEITSLNKAFIIGISLNLAFVVAEFTAGFWYNSLG
                     LLSDAGHNLGDVASLVLAMLAFRLAKVHPTCSYTYGYKKSTVLVSLLNAVILLVAVGII
                     IAESIGKLLHPRPVEGDAIAWVAGIGVVINALTAWLFMKDKDKDLNVKGAYLHMAADAL
                     VSVGVVVSGIVIAHTGWYIIDPIIGLLIAIVIIVSTWGLLRDSIRLSLDGVPAGTDIDK
                     VEKTIHSVEGVEGVHHLHIWAISTTETALTAHVSVADISEMERIKQEIKHDLQHVGIQH
                     ATLEFETSGFKCDGHCCSD"
     CDS             3630..4196
                     /locus_tag="ctg328_4"
                     /transl_table=11
                     /translation="MEPIINSQAPEFKVQAYHNGSFKTVSSDDIKGKWAIFFFYPADFT
                     FVCPTELVDMADKYDQFQAMGVEVYSVSTDSHFVHKAWHDASETIRKIKYPMLADPTGV
                     LSRAFGVMIEEDGMAYRGTFLVNPEGKIKLAEINDNGIGRNADELLRKVEAAQFIAEHP
                     SEVCPAKWKKGGETLKPSIDLVGKI"
     CDS             4290..5228
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) GIDA"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg328_5"
                     /sec_met_domain="GIDA (E-value: 7.1e-09, bitscore: 23.5,
                     seeds: 122, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MLDSSMRNQLSGIFSGLTNEYIFDIQVSPEHESRKELLELLNDVA
                     SCSDKITVQVADGEGLEFSLLKNGEKTGIKFRGVPNGHEFTSLLLAILNSDGKGKNIPD
                     ESICNRVKALNGPVKLTTYVSLTCTNCPDVVQALNAMATLNPEISHEMVDGAINQEEVA
                     AMKVQGEPSVFADGKLIHVGRGDFGELLGKLEAQYGVKEENLSVVEKNYDVIIVGGGPA
                     GSAAAIYTARKGLAVAVVADRIGGQVKETVGIENMISVSQTTGQQLALDLMSHMKDYPI
                     DILEHRRIEKVEVEGREKLLTTSTGEKLIAPA"
ORIGIN
        1 cttacggacg cggacgtatt atcggtgact accgccgtgt agcactttac ggtacggaaa
       61 tcctgatcgc tgaaaagaaa cattatctga aaagactcga tattcaggaa ctgaccgaag
      121 agatcatcca gagccgtgag gaaatatccg aacagatcaa ggcgctgaag gctttcgaaa
      181 agatgtgtgc cggttatggt ttcgacgtga ccaagcctgc aaaggatgca cgcgaagcgg
      241 ttcagtttgt ctatctgggt tatctggctg cggtgaagga ccaggatggg gctgccatgt
      301 cgttgggacg tacttctact ttcctcgata tttatattga aaaagatatc cgcgaaggca
      361 agctgacgga agaagaagct caggagctga tcgaccagtt tattatcaaa ctgcgtatcg
      421 tccgtttcct tcgtactccg gaatataacg acctgttctc cggtgaccct gtttgggtaa
      481 ccgagtcttt gggcggacag ggtgtcgacg gccgttcatt ggtgacgaag acatcttacc
      541 gttatctgca tacattatat aacctcggcc cggcaccgga acccaactta acggtactgt
      601 ggttcaacaa cgctcctgaa aactggaaac gtttctgtgc gaaggtttct atcgatactt
      661 ccgctatcca gtatgagaac gacgatctga tgcgtccgga ttatggagat gattatggta
      721 ttgcctgctg cgtgtctccg atgaagatcg gtaagcagat gcagtatttc ggcgcacgtg
      781 ccaacctggc taaatgtttg ctgtatgcca tcaacggcgg gcgcgatgaa agatcaggtg
      841 tacaggtggc tccgaggttt gaaccgatca ccagcgaata cctggattac aacgaagtga
      901 tggagaaata cgaacagatg atgcgctggc tggctaaagt ttatgtgaac gcactgaaga
      961 tcatccatta catgcacgat aaatatgcgt atgaagcatt cgagatggct ttgcatgacg
     1021 gttcggtaga acgtatccgc gcaacaggta ttgccggcct ttctatcgtt gccgactcat
     1081 tggctgctat ccgcgatacg aaagtaaaag tgatccgcga cgaacgtggc ctggctgtcg
     1141 gtttcgaacg tgaaggcgat tatgttcctt tcggtaacaa tgacgatcgt acagatagta
     1201 ttgctgtcga catcactgaa aagttcatgg aatatctgcg tcagcaccag acataccgta
     1261 atgcaactcc gacacagtct atcctgacta ttacgtctaa tgtcgtatac ggaaagaaga
     1321 caggtgcaac tcccgacggt cgcgaaggtg gtacgccgtt tgctccgggt gcgaacccga
     1381 tgaacggacg tgataccaaa ggcgctgttg ctgcactggc ttctgttgct aaactgccgt
     1441 tccagcatgc acacgacggt atctcttata cattcgctgt ttctcctgca acgttaggaa
     1501 aagaacgtga tattcagatc gccaatatgg tcggcctgct ggacggttac tttactcctg
     1561 acggcggaca gcatctgaat gtgaacgtat tcgacaagga gctgttgctc gatgctatgg
     1621 agcatccgga aaactatccg cagttgacaa tccgtgtatc gggttatgca gtaaactttg
     1681 tgaaactgac acgcgaacag cagctggatg tgatttcaag aacaatcaat cattctttgt
     1741 aattgaaagt tggaaattaa aggaaacata cattcactag agagtttcgg gaccgttgat
     1801 ggtcccggaa ttcgttttgt tgtctttatg caggggtgtc cgctccgttg cctgtattgc
     1861 cacaatccgg atacctggga tagcaaaggg cttgccaaat atcagatgac gcctgaagaa
     1921 ttattggctg aggttttacg atataagaac tttatcgcca agggtggcgt gactgtcacc
     1981 gggggagaac ccttattgca agctgaattt ctcagggagt ttttccgtct ttgccgggaa
     2041 gccggtattc atacggcgct cgatacatcc ggctatatct gcacaccgaa agccctggcg
     2101 gttctggaat atgccgacct ggtcttattg gatataaaaa ccatcgatcc cgacctgcat
     2161 ccccgtctaa cggccgtgaa actggataat acgttacgtt tcctggacga actggaaaaa
     2221 cgcggtgtcc cggtttggat acgccatgtc attgtgcccg gactgacgga taatgacgaa
     2281 gccttggata aactggccga atacatcagc cactatacgg ttgtacagaa ggcggaactc
     2341 ttgccttatc acacgatggg cgcttataaa tacgaggcac aaggtctcga ctacaaactg
     2401 aaagaggtag aacctttatc ggccgaacgt ctggcaaatg ccaaagaaat atttaagaag
     2461 cacggtataa ccatctggta atttacctat aatcaggtat atttttattg ctctaaaaaa
     2521 catagttttg tcccatattg ttactcaaat aaaagtagga atgaatatgg aacaacatca
     2581 tcacgatcat caccaccacg aaataacctc tttgaacaag gctttcatta taggtatttc
     2641 cctgaacctg gcttttgtcg tagccgaatt tacggctggc ttctggtata attcattagg
     2701 acttttgtcg gacgcagggc ataacctggg agatgtagcc agcctggtac tggctatgct
     2761 ggctttccgg ctggctaagg tgcacccgac ttgcagctat acctatggat ataagaaaag
     2821 cactgtactc gtatcccttc tgaatgcggt catcctcttg gtggccgtcg ggattattat
     2881 tgctgaaagt ataggaaaac tgctgcatcc gcgtccggtc gagggtgatg cgatcgcttg
     2941 ggtggcaggt atcggagtcg ttataaatgc acttacggca tggcttttta tgaaggataa
     3001 ggataaagac ttgaatgtga aaggtgctta cttacatatg gctgccgatg cactggtatc
     3061 ggtcggcgtg gttgtctccg gaattgttat tgcccatacc ggatggtata tcatcgaccc
     3121 gattatcggc ctgttgattg cgatcgttat cattgtttct acctggggac tgctgcgcga
     3181 cagtatccgt ctctctctgg acggtgtgcc ggcgggaacg gacatcgata aggtggaaaa
     3241 gacgatacat tcggttgaag gcgtcgaagg tgtccaccat cttcatatct gggcgatcag
     3301 tacgaccgaa acggcactta ccgcccatgt ttccgttgcg gatatttcag aaatggaacg
     3361 tatcaaacag gagataaaac acgaccttca gcatgtggga atccagcatg ctacgctcga
     3421 atttgaaacg tcgggattca agtgcgatgg gcactgttgc tcagactgaa acaggtatat
     3481 tataaacctg atttactgat actcaaagat tttgtctatg actgcataaa acctttcgat
     3541 tggatttttt gttcttacct cgaatcggca acttatcttt gcaccagtaa caaaaacaaa
     3601 atagtaataa cgattaaaat aaaaattcta tggaacctat tatcaattca caagcacccg
     3661 agtttaaagt tcaggcatac cacaacggta gtttcaaaac tgtaagcagc gacgacatca
     3721 aaggcaaatg ggcgattttc tttttctacc cggctgactt tacatttgtt tgtccgaccg
     3781 agttggtaga tatggctgac aaatatgacc agttccaggc aatgggtgta gaagtttatt
     3841 ctgtaagtac agattcacac ttcgtacaca aagcatggca cgatgcatct gaaacgattc
     3901 gtaagatcaa atacccgatg ttggcagacc cgacaggcgt attgagccgt gcattcggtg
     3961 taatgatcga agaagacggt atggcttacc gcggtacatt cctggtaaat ccggaaggaa
     4021 agatcaaact ggcagaaatc aacgataacg gtatcggccg taacgccgac gaactgcttc
     4081 gtaaagtaga agctgcccag ttcatcgccg aacatccgag cgaagtttgc cccgccaaat
     4141 ggaaaaaagg tggcgaaaca ttgaaaccga gcatcgatct ggtcggtaag atctaatagt
     4201 aaaactggag gccggcaaca tattgtcggc cttttttatc taaacaaata tcagttgttc
     4261 tattcataat taaaataaag gattcaacta tgttagattc atcaatgaga aatcagttgt
     4321 ccggtatctt tagcggattg acgaacgaat atatatttga tatacaagtt tctcctgaac
     4381 atgaaagcag gaaggaacta ttggaattgc tgaacgatgt ggcttcctgt tccgataaga
     4441 tcaccgtaca ggtggcagac ggcgaaggcc tggagttctc cctgctgaaa aacggggaaa
     4501 aaaccggtat taaattccgg ggcgttccca acggacacga atttacttcg ttgctgctgg
     4561 ctatcctgaa cagtgacggc aaaggcaaaa atattcccga cgaaagtatt tgtaaccgtg
     4621 tgaaggccct gaatggcccg gtcaaactga ctacatatgt ttctttgacc tgtaccaact
     4681 gtcctgacgt tgtacaggct ttgaatgcca tggctacact gaacccggaa atctctcacg
     4741 aaatggtgga cggtgcgatc aaccaggaag aggttgctgc catgaaagtg cagggagaac
     4801 cttccgtatt tgccgacggt aaactgatcc atgtaggtcg tggagacttt ggcgaactgc
     4861 ttggcaaact cgaagcgcag tatggcgtga aagaggagaa cctgtcggtg gtagaaaaga
     4921 actacgatgt tatcatcgtc ggcggaggtc ctgccggaag tgcggcagct atctatactg
     4981 cccgtaaagg tttggcggtg gctgttgtgg ccgaccgtat cggcggacag gtgaaggaaa
     5041 ctgtcggtat cgagaatatg atttctgttt cgcagacgac ggggcagcag ctggctctcg
     5101 acctgatgtc gcatatgaaa gattatccga tcgatatcct tgaacaccgc cgtatcgaga
     5161 aggtggaagt cgaaggacgc gaaaagctgc tgacaacctc taccggcgaa aaactgatag
     5221 ccccggccgt
//
