LOCUS       c00182_gnlPro..         5264 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|EFCCIFOG_182.
ACCESSION   c00182_gnlPro..
VERSION     c00182_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-05 13:50:21
            Original ID  :: gnl|Prokka|EFCCIFOG_182
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 5264
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..5264
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[1560:4540]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      1561..4540
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..5264
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..5264
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             204..812
                     /locus_tag="ctg182_1"
                     /transl_table=11
                     /translation="MITQYDLYETPSPKGEDGKKSLHARVCPKKTYTSQEFVEHVAAFE
                     HLPKNQIGGALAAIVDDLCYLLADGNIVELGDLGFFSTSLKCLKDTDDDEDEIKPKSIA
                     FQNVHLRISSEFRKRIKHEMKFQRVHSLTRRPKIVNSTVKERMKKMVSFLKINICITRK
                     EYIGLVGLTPYEAMNELNDFISQGLLRRRGAGRSVVFSL"
     CDS             1561..3801
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate2"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg182_2"
                     /sec_met_domain="Gly_radical (E-value: 1.1e-45, bitscore:
                     142.8, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate2 (E-value: 3.4e-285, bitscore:
                     935.1, seeds: 33, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKFENENWAQFKGEIWKREINVRDFIQNNYKPYEGDDSFLKDSSD
                     KTKKVWDKLTEMFKVEREKGVYDAETKYPQGIDTYGPGYIDKENEVIVGLQTDAPLKRG
                     IYPKCGTRMVENSLEAYGYKLDPMTKEIFTKYRKTHNEGVFSAYTDEMLAARRSAIITG
                     LPDAYGRGRIIGDYRRVALYGTEILIAEKKHYLKRLDIQELTEEIIQSREEISEQIKAL
                     KAFEKMCAGYGFDVTKPAKDAREAVQFVYLGYLAAVKDQDGAAMSLGRTSTFLDIYIEK
                     DIREGKLTEEEAQELIDQFIIKLRIVRFLRTPEYNDLFSGDPVWVTESLGGQGVDGRSL
                     VTKTSYRYLHTLYNLGPAPEPNLTVLWFNNAPENWKRFCAKVSIDTSAIQYENDDLMRP
                     DYGDDYGIACCVSPMKIGKQMQYFGARANLAKCLLYAINGGRDERSGVQVAPRFEPITS
                     EYLDYNEVMEKYEQMMRWLAKVYVNALKIIHYMHDKYAYEAFEMALHDGSVERIRATGI
                     AGLSIVADSLAAIRDTKVKVIRDERGLAVGFEREGDYVPFGNNDDRTDSIAVDITEKFM
                     EYLRQHQTYRNATPTQSILTITSNVVYGKKTGATPDGREGGTPFAPGANPMNGRDTKGA
                     VAALASVAKLPFQHAHDGISYTFAVSPATLGKERDIQIANMVGLLDGYFTPDGGQHLNV
                     NVFDKELLLDAMEHPENYPQLTIRVSGYAVNFVKLTREQQLDVISRTINHSL"
     CDS             3809..4540
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 72.3;
                     E-value: 1.6e-22)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg182_3"
                     /sec_met_domain="Radical_SAM (E-value: 3.7e-37, bitscore:
                     116.4, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="LEIKGNIHSLESFGTVDGPGIRFVVFMQGCPLRCLYCHNPDTWDS
                     KGLAKYQMTPEELLAEVLRYKNFIAKGGVTVTGGEPLLQAEFLREFFRLCREAGIHTAL
                     DTSGYICTPKALAVLEYADLVLLDIKTIDPDLHPRLTAVKLDNTLRFLDELEKRGVPVW
                     IRHVIVPGLTDNDEALDKLAEYISHYTVVQKAELLPYHTMGAYKYEAQGLDYKLKEVEP
                     LSAERLANAKEIFKKHGITIW"
     CDS             4626..5264
                     /locus_tag="ctg182_4"
                     /transl_table=11
                     /translation="MEQHHHDHHHHEITSLNKAFIIGISLNLAFVVAEFTAGFWYNSLG
                     LLSDAGHNLGDVASLVLAMLAFRLAKVHPTCSYTYGYKKSTVLVSLLNAVILLVAVGII
                     IAESIGKLLHPRPVEGDAIAWVAGIGVVINALTAWLFMKDKDKDLNVKGAYLHMAADAL
                     VSVGVVVSGIVIAHTGWYIIDPIIGLLIAIVIIVSTWGLLRDSIRLSLDG"
ORIGIN
        1 tcccttcgtt taaaataaaa aatcgttcga caatcattct tcatgcattt aacagctatc
       61 gaataatcat tcgacaattg tcgaacgaat aaagaatgat tgtcgaatga ttttttttct
      121 agaagcgaaa tttattttta gaagtatttc gatttatcag ggagaaacaa gttatatttg
      181 aacaattaat atcaatataa cctatgatca cacaatacga cctgtatgaa acaccttcac
      241 ccaaagggga agacggtaaa aaatcgctgc atgcccgtgt ttgtccgaaa aagacttaca
      301 ccagtcaaga gtttgtagaa catgtggcag cctttgaaca tctgccgaag aatcagattg
      361 gcggggcact tgctgccatt gtcgacgatc tttgttatct gcttgccgat ggaaacattg
      421 tggaattagg tgatcttgga tttttttcta cttcattgaa gtgcctgaag gatacggatg
      481 acgatgagga tgaaatcaaa ccgaaatcga tcgcttttca aaatgtacat ctgagaatca
      541 gcagtgaatt ccgaaaaaga atcaaacatg agatgaagtt tcaacgcgtg cattccctta
      601 cgcggagacc aaaaattgtc aattctaccg tgaaagagcg gatgaaaaaa atggtctctt
      661 tcctcaaaat aaacatttgt attacccgaa aggaatatat aggactggtc ggattgacac
      721 cttacgaggc gatgaatgaa ctgaacgatt ttatcagcca ggggcttctt cgccgtcgcg
      781 gggcgggcag gtctgtggta ttttccctgt aatccctatt tatggttaga aatgagctcg
      841 ttttacatat atataggtat tgcctgtgcc tttccgtggt tttacctttg ccgtatcaat
      901 taagttttac tattagtata aatcgtcctg accaaaatcc gatttgttga tttttatgta
      961 cacctattat ttaaggtaaa tacccatgga agggagtttt tttattggtt gtccctttca
     1021 tatataaaag ggagccatcc tgtgaagaat ggctcttttt tatttatcaa agaaagcaat
     1081 ttttgcaaat attgtttatt ccaatgatca attttcccga ttataacaca gaataggtag
     1141 taggaagctc tttttttact atataatagg tattgtgtac tccccttgac ggctctatct
     1201 ttgcaacata gaaaaaagta acactattag tataatcgct atatcaaaac cttttgctaa
     1261 ttatgtaagc atataagatg attaaacgtg tgtcggaaat tttttattgg ttgtttccgg
     1321 cataaaaaag ggactctcct gcgaaggatg gttccttttt tatttttttg atattattta
     1381 taatccttcc tgtttgatac ctatatcagg cgatacgctg cactatatct accgttttag
     1441 taactatttg tagggatttt ggccgttggg aatacatgat gtttttgttg attgtttcgc
     1501 atgccatata aataacgtac ctttgcaccg aaattaaaac cgaagaaaac aaaataaggt
     1561 atgaagtttg aaaacgagaa ctgggcgcag ttcaaaggcg aaatttggaa aagagagatt
     1621 aacgtaaggg attttatcca gaacaactac aaaccttacg aaggggacga ctctttcttg
     1681 aaagactcct cagataagac gaagaaagtt tgggataaat tgaccgaaat gtttaaggtt
     1741 gaaagggaga agggtgttta cgacgctgaa acaaaatatc ctcaaggtat agatacttac
     1801 ggaccaggtt atattgacaa agaaaacgaa gtaattgtcg gcctgcaaac cgatgccccc
     1861 ctgaaacgcg gtatttaccc gaaatgtggt acccgcatgg tggaaaacag tctggaagca
     1921 tatggctaca aactggaccc gatgacaaaa gaaatcttca cgaaatacag aaagacgcat
     1981 aacgaaggcg tattctccgc ttatacagat gaaatgcttg cagcgcgccg ttccgctatc
     2041 atcaccggcc tgccggatgc ttacggacgc ggacgtatta tcggtgacta ccgccgtgta
     2101 gcactttacg gtacggaaat cctgatcgct gaaaagaaac attatctgaa aagactcgat
     2161 attcaggaac tgaccgaaga gatcatccag agccgtgagg aaatatccga acagatcaag
     2221 gcgctgaagg ctttcgaaaa gatgtgtgcc ggttatggtt tcgacgtgac caagcctgca
     2281 aaggatgcac gcgaagcggt tcagtttgtc tatctgggtt atctggctgc ggtgaaggac
     2341 caggatgggg ctgccatgtc gttgggacgt acttctactt tcctcgatat ttatattgaa
     2401 aaagatatcc gcgaaggcaa gctgacggaa gaagaagctc aggagctgat cgaccagttt
     2461 attatcaaac tgcgtatcgt ccgtttcctt cgtactccgg aatataacga cctgttctcc
     2521 ggtgaccctg tttgggtaac cgagtctttg ggcggacagg gtgtcgacgg ccgttcattg
     2581 gtgacgaaga catcttaccg ttatctgcat acattatata acctcggccc ggcaccggaa
     2641 cccaacttaa cggtactgtg gttcaacaac gctcctgaaa actggaaacg tttctgtgcg
     2701 aaggtttcta tcgatacttc cgctatccag tatgagaacg acgatctgat gcgtccggat
     2761 tatggagatg attatggtat tgcctgctgc gtgtctccga tgaagatcgg taagcagatg
     2821 cagtatttcg gcgcacgtgc caacctggct aaatgtttgc tgtatgccat caacggcggg
     2881 cgcgatgaaa gatcaggtgt acaggtggct ccgaggtttg aaccgatcac cagcgaatac
     2941 ctggattaca acgaagtgat ggagaaatac gaacagatga tgcgctggct ggctaaagtt
     3001 tatgtgaacg cactgaagat catccattac atgcacgata aatatgcgta tgaagcattc
     3061 gagatggctt tgcatgacgg ttcggtagaa cgtatccgcg caacaggtat tgccggcctt
     3121 tctatcgttg ccgactcatt ggctgctatc cgcgatacga aagtaaaagt gatccgcgac
     3181 gaacgtggcc tggctgtcgg tttcgaacgt gaaggcgatt atgttccttt cggtaacaat
     3241 gacgatcgta cagatagtat tgctgtcgac atcactgaaa agttcatgga atatctgcgt
     3301 cagcaccaga cataccgtaa tgcaactccg acacagtcta tcctgactat tacgtctaat
     3361 gtcgtatacg gaaagaagac aggtgcaact cccgacggtc gcgaaggtgg tacgccgttt
     3421 gctccgggtg cgaacccgat gaacggacgt gataccaaag gcgctgttgc tgcactggct
     3481 tctgttgcta aactgccgtt ccagcatgca cacgacggta tctcttatac attcgctgtt
     3541 tctcctgcaa cgttaggaaa agaacgtgat attcagatcg ccaatatggt cggcctgctg
     3601 gacggttact ttactcctga cggcggacag catctgaatg tgaacgtatt cgacaaggag
     3661 ctgttgctcg atgctatgga gcatccggaa aactatccgc agttgacaat ccgtgtatcg
     3721 ggttatgcag taaactttgt gaaactgaca cgcgaacagc agctggatgt gatttcaaga
     3781 acaatcaatc attctttgta attgaaagtt ggaaattaaa ggaaacatac attcactaga
     3841 gagtttcggg accgttgatg gtcccggaat tcgttttgtt gtctttatgc aggggtgtcc
     3901 gctccgttgc ctgtattgcc acaatccgga tacctgggat agcaaagggc ttgccaaata
     3961 tcagatgacg cctgaagaat tattggctga ggttttacga tataagaact ttatcgccaa
     4021 gggtggcgtg actgtcaccg ggggagaacc cttattgcaa gctgaatttc tcagggagtt
     4081 tttccgtctt tgccgggaag ccggtattca tacggcgctc gatacatccg gctatatctg
     4141 cacaccgaaa gccctggcgg ttctggaata tgccgacctg gtcttattgg atataaaaac
     4201 catcgatccc gacctgcatc cccgtctaac ggccgtgaaa ctggataata cgttacgttt
     4261 cctggacgaa ctggaaaaac gcggtgtccc ggtttggata cgccatgtca ttgtgcccgg
     4321 actgacggat aatgacgaag ccttggataa actggccgaa tacatcagcc actatacggt
     4381 tgtacagaag gcggaactct tgccttatca cacgatgggc gcttataaat acgaggcaca
     4441 aggtctcgac tacaaactga aagaggtaga acctttatcg gccgaacgtc tggcaaatgc
     4501 caaagaaata tttaagaagc acggtataac catctggtaa tttacctata atcaggtata
     4561 tttttattgc tctaaaaaac atagttttgt cccatattgt tactcaaata aaagtaggaa
     4621 tgaatatgga acaacatcat cacgatcatc accaccacga aataacctct ttgaacaagg
     4681 ctttcattat aggtatttcc ctgaacctgg cttttgtcgt agccgaattt acggctggct
     4741 tctggtataa ttcattagga cttttgtcgg acgcagggca taacctggga gatgtagcca
     4801 gcctggtact ggctatgctg gctttccggc tggctaaggt gcacccgact tgcagctata
     4861 cctatggata taagaaaagc actgtactcg tatcccttct gaatgcggtc atcctcttgg
     4921 tggccgtcgg gattattatt gctgaaagta taggaaaact gctgcatccg cgtccggtcg
     4981 agggtgatgc gatcgcttgg gtggcaggta tcggagtcgt tataaatgca cttacggcat
     5041 ggctttttat gaaggataag gataaagact tgaatgtgaa aggtgcttac ttacatatgg
     5101 ctgccgatgc actggtatcg gtcggcgtgg ttgtctccgg aattgttatt gcccataccg
     5161 gatggtatat catcgacccg attatcggcc tgttgattgc gatcgttatc attgtttcta
     5221 cctggggact gctgcgcgac agtatccgtc tctctctgga cggt
//
