LOCUS       c00532_gnlPro..         2401 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|CIIEBGGC_532.
ACCESSION   c00532_gnlPro..
VERSION     c00532_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-07 21:37:52
            Original ID  :: gnl|Prokka|CIIEBGGC_532
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 2401
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..2401
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[1:2219]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      2..2219
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..2401
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..2401
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             2..802
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate2"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg532_1"
                     /sec_met_domain="Gly_radical (E-value: 8.5e-16, bitscore:
                     46.7, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate2 (E-value: 1.3e-83, bitscore:
                     269.5, seeds: 33, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="GKEMQFFGARANLAKCLLYALNGGVDEVTKKQVGPKYRAVVGDVL
                     DFNDVWDKFIDMMEWQADVYVNTLNIIHYMHDKYCYERAEMALHDRDVKRYFATGVAGL
                     SIVADSLSAIKYAKVEVVRDEDGVIVDFNTTGEFPKYGNDDDRVDEIAQEVVHRFMTAV
                     RRHHTYRNGVPTTSILTITSNVTYGKATGNTPDGRKKGQPFAPGANPMHGRDTHGAVAS
                     LSSVSKLPFKDAQDGISNTFTIIPGALGKEDQVFAGDLELDLNK"
     CDS             831..1034
                     /locus_tag="ctg532_2"
                     /transl_table=11
                     /translation="MDDNKMIDDLVKMLDSGMMQGVGHINVDTDNSMEESKNVQTLGCT
                     DCSRTPLACSVPTLEQGLDDYH"
     CDS             1109..1351
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg532_3"
                     /sec_met_domain="Gly_radical (E-value: 2.3e-28, bitscore:
                     87.1, seeds: 99, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MATSSAQVDNLVNLLDGYATKGGHHLNVNVLNRDMLLDAQKHPEN
                     YPQLTIRVSGYAVNFIKLTPEQQADVISRTFHESI"
     CDS             1470..2219
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 77.9;
                     E-value: 2.9e-24)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg532_4"
                     /sec_met_domain="Radical_SAM (E-value: 6.5e-36, bitscore:
                     112.4, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MQGRIHSLESFGTVDGPGTRFVVFVQGCPMRCAYCHNPDTWPMDG
                     GTMMEPEEIFEQYKRNQPFYKTGGITVTGGEPLMQVDFLIDLFTIAKKENVHTCIDTSG
                     IAYKPQNTEWIEKLDRLMALTDLVMLDIKHIDPEKHQELTSQPNDGILAFASYLNEKQV
                     DMWIRHVVVPGITDDDKYLFELGYFIGQFSNLKAIDVLPYHTMGETKYEKLDMTYKLAG
                     VPAMDKEKVVDKKQVILDGIKKRRSEA"
ORIGIN
        1 cggtaaggaa atgcagttct tcggcgcacg cgcaaacctt gcaaaatgtc tgctttacgc
       61 tctgaacggc ggcgtggacg aagtaacaaa gaaacaggtt ggaccgaagt accgcgccgt
      121 tgtaggcgat gttcttgact ttaacgatgt atgggataaa ttcatcgaca tgatggaatg
      181 gcaggcagac gtatatgtaa atactctgaa catcatccat tacatgcatg acaaatactg
      241 ctatgaaaga gctgagatgg cgcttcatga cagagacgta aaacgttact ttgcaacggg
      301 tgttgcaggg ctttccatcg tagcagactc cttgtccgct attaaatatg caaaagttga
      361 agttgtcaga gatgaagacg gcgttatcgt agatttcaat acaaccggcg aattcccgaa
      421 atacggcaat gatgacgacc gcgttgacga aatcgcgcag gaagtggttc acagattcat
      481 gacagcagtc agaagacacc acacctacag aaacggtgtt cctaccacct ctatccttac
      541 cattacttcc aacgtaacat atggtaaggc tacaggaaac acacctgacg gacgtaagaa
      601 agggcagcct tttgcacctg gcgctaaccc gatgcacggc cgcgatactc acggtgcggt
      661 agcttcctta tcttccgtat ccaagcttcc gttcaaagat gctcaggatg gtatttccaa
      721 taccttcacg atcatccccg gcgctcttgg aaaagaagac caggtatttg caggcgacct
      781 tgaactcgac ttgaacaaat aatttttcac agaaataacg agggttttct atggacgaca
      841 ataaaatgat cgacgatctc gttaaaatgt tggactccgg catgatgcag ggtgtgggtc
      901 atatcaacgt agacacggat aattctatgg aagaatccaa aaacgttcag actttgggat
      961 gcacagactg ctcccggacc ccgttagcat gcagtgttcc tacattggaa cagggcttgg
     1021 acgattacca ctaaaaacca tatggctgcg gcccggcatt ccgctttgcc gcagcctgtc
     1081 aataaagatc ttaaggagga aacttatcat ggcaacaagt tcggcacagg ttgataacct
     1141 tgtaaactta ttagatggat acgcaacaaa aggcggacat caccttaacg ttaatgtatt
     1201 aaacagagat atgcttctgg atgcccagaa acatcctgag aactatcccc agctgacgat
     1261 ccgtgtatcc ggatatgctg taaacttcat caaactgaca ccggaacagc aggcggatgt
     1321 tatctcccgt actttccatg aatctatcta atagaagcta tttaaaagtt tgacaaagca
     1381 aagagttggc ataacggcca actcttcgtt tttctccgac ttttgctata tgaaataaaa
     1441 agagtatcac tcaaaaggag atgaactata tgcaaggaag aatacattca ttagaaagct
     1501 tcggaaccgt agacggcccc ggcacccgat tcgtcgtctt tgtacaagga tgccctatgc
     1561 gctgtgccta ctgccacaac cccgacacgt ggcctatgga cggaggcacg atgatggaac
     1621 cggaagaaat cttcgaacaa tacaagcgca accagccttt ttataaaacc ggtggaatta
     1681 ccgtaaccgg cggagagcct cttatgcagg tcgatttcct catcgactta ttcacgatcg
     1741 ccaaaaaaga gaatgtccat acctgcatcg acacttccgg catcgcatat aaaccgcaaa
     1801 atacggaatg gatcgaaaaa ctggatcgtt taatggcctt aaccgatctc gtaatgcttg
     1861 acatcaaaca tattgatccc gaaaaacatc aagaattgac atcccagccc aacgacggta
     1921 ttctggcttt tgcctcctac ctcaacgaaa agcaggtgga catgtggatt cgccatgttg
     1981 ttgtccccgg tatcaccgat gatgataaat atcttttcga actcggatac tttatcgggc
     2041 aattcagcaa tttaaaagcc atcgacgtac ttccctacca taccatggga gagaccaaat
     2101 atgaaaaact ggacatgact tataagcttg ccggagtacc cgccatggac aaggaaaaag
     2161 tcgttgataa aaagcaggta atcctggacg gcatcaaaaa acgccgcagt gaagcctgat
     2221 ctctccggct gcaaaatgaa cggtaaccgc ctgatatttc ctttgatttt ctaaaaggtt
     2281 cctcttgtat ttacacatat tttatattaa aatagataca gagtttgata acaatttatc
     2341 aataaggata cacatattaa ggaggattta attatggcat acgtaattgg tgacgcttgt
     2401 g
//
