LOCUS       c00242_gnlPro..         4554 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|EBIJBGDG_242.
ACCESSION   c00242_gnlPro..
VERSION     c00242_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-05 15:58:09
            Original ID  :: gnl|Prokka|EBIJBGDG_242
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 4554
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..4554
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[598:3554]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      599..3554
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..4554
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..4554
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             599..2827
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate1"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg242_1"
                     /sec_met_domain="Gly_radical (E-value: 1.7e-47, bitscore:
                     148.2, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate1 (E-value: 0.0, bitscore:
                     1171.1, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MELNKTFTNGLWNKEINVSDFVSKNIAPYTGDASFLQGPTERTKR
                     IWDICLKALEEERNNNGVRSLDNTTVSTITSHKAGYIDKDNELIVGLQTDELLKRSIKP
                     FGGINVVAKACRENGVEVDDKVKDIFTHYRKTHNDGVFDVYTEEIRSFRSLGFLTGLPD
                     NYARGRIIGDYRRLALYGIDRLIEAKQEDLRNLTGPMTEARIRLREEVAEQIKALKDIK
                     VMGEYYGLDLSHPATSAQEAVQWVYMAYLAAIKEQDGAAMSLGNVSSFLDIFIEYDLAH
                     GKINETFAQELIDQFVIKLRMVRHLRMQSYNDIFAGDPTWVTEAIGGRFNDGRVKVTKT
                     SFRFLQTLYNLGPSPEPNLTVLWSPELPEGFKDFCAKVSVDTSSIQYENDNLMREVRNC
                     DDYGIACCVSYQAIGKQIQFFGARANLAKALLLAINGGRCENTGTVMVKGIPVLTGETL
                     KFEEVMANYKKVLTEIARVYNEAMNIIHYMHDKYYYEKAQMAFIDTNPRINLAYGVAGL
                     SIAIDSLSAIKYAKVTARRNEIGLTEGFDTEGSFPCFGNNDDRVDHLGVDLVYYFSEEL
                     KKLPVYKNARPTLSLLTITSNVMYGKKTGATPDGRAKGVAFAPGANPMHGRDKNGAIAS
                     LSSVAKLRYRDSQDGISNTFSIVPKSLGPTPEERVENLVTMMDGYFTKGAHHLNVNVLN
                     REMLEDAMEHPEKYPQLTIRVSGYAVNFVKLSREHQLEVISRSFHERM"
     CDS             2829..3554
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 75.5;
                     E-value: 1.7e-23)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg242_2"
                     /sec_met_domain="Radical_SAM (E-value: 5.5e-32, bitscore:
                     99.2, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MINVHSYESMGTFDGPGLRLVVFLQGCPFRCLYCANPDTIDVKGG
                     TPTTPEEILQMAISQKAFFGKKGGITFSGGEPTLQAEALIPLFKELKANGIHICLDSNG
                     GIWNEKVEELFSLTDLVLLDIKQFNPERHRTLTGRSNEQTLRTAAWLEEHEHPFWLRYV
                     LVPGYSDSEEDIRLLGESLGKYRQIQRVEILPYHRLGVHKYEVMGWDYKLKEVKENTPE
                     QLERAEKLFKEYFTNVITN"
     CDS             complement(3623..4552)
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Fn3-like"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Glyco_hydro_3_C"
                     /gene_functions="other (smcogs) SMCOG1041:Fibronectin type
                     III-like domain (Score: 88.9; E-value: 6.1e-28)"
                     /gene_kind="other"
                     /locus_tag="ctg242_3"
                     /sec_met_domain="Fn3-like (E-value: 2.9e-29, bitscore:
                     88.9, seeds: 729, tool: rule-based-clusters)"
                     /sec_met_domain="Glyco_hydro_3_C (E-value: 3.1e-43,
                     bitscore: 135.8, seeds: 116, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="ATTDRSKVVTLYDALVRKGLEVNYAEGYDMKSNQLTGLQEALQAA
                     EASDVIVVAVGERFGQSGEKASKVNIAIPEGQQKLVSELAKTGKPVIALVMCGRPVIFN
                     EIREHADAVLCTWWLGSEAGNAICNVLWGEYNPSAKLPMTFPAHVGQIPIYYQNKSTGR
                     PTALQQTYKASYIDASTEPAYPFGYGLSYTDFGYSDLKLLPGKDAGVHTEVSVKVTNTG
                     KCAGEEVVQLYVQDKVASVTRPIKELKGFRKIHLNAGESKTVAFQITDDALGFYDNEMN
                     YMVEPGDFVFMVGGSSDDVQQITYNLKE"
ORIGIN
        1 caacggtttt caagaccgcc gcaatcgacc actctgccac ctctccaaaa cttctttttc
       61 agaagtgctt ttcttttaaa gcggtgcaaa gatacaaatc attttttatt ctacaaactt
      121 ttctgcacaa aaacagagca attataccaa aattcttgtt tatacccact acggaacgaa
      181 cataacggca tttaggctat ttgttgaaat ttatcaaggg caagacagag ttttataact
      241 tactggctat cagtatattt acaaagaacc acattcagta tacgattgaa ccctattcag
      301 tatacgactg aatggcattc actatacgac tgaattaggt tcagaatacg actgttcctt
      361 aaacctacat catttgtaaa taatgcagat tattattcgg ttcttgtttc aattcataaa
      421 ccacatatag aaagtattaa catctctact cccccggaca ttcactacct aataatagtt
      481 attttatgct gttcaacata aaaagagaag tatctcttat aaaattacgg aatttattca
      541 gtatttttgt gcgctctaaa ggataacatt atctaaccta ctaacaaaaa caaattttat
      601 ggaattgaac aaaaccttta ccaatgggct ttggaataaa gaaatcaatg tcagtgattt
      661 cgtcagtaaa aacatcgcac cctataccgg ggatgcatct ttcttgcagg gacctaccga
      721 acgtaccaaa cgtatctggg acatctgtct gaaggctctc gaagaagaaa ggaacaacaa
      781 tggcgtccgt tcactggaca ataccaccgt atctaccatc acttctcata aagccgggta
      841 tattgataag gacaatgaac ttattgtcgg tttgcagact gatgaactgt tgaaacgatc
      901 catcaaacct ttcggaggca tcaacgtggt agcaaaagca tgccgtgaaa acggagtgga
      961 agtggacgat aaagtaaaag acatcttcac ccattaccgc aaaacacata acgacggcgt
     1021 attcgatgta tataccgaag aaatccgttc cttccgctca ctgggattcc ttaccggatt
     1081 gcccgacaac tatgcccgcg gacgtatcat cggcgactac cgccgattgg ctctctacgg
     1141 catcgaccgt ctgattgaag ccaagcagga ggacttgcgc aacctcaccg gcccgatgac
     1201 cgaagcacgc atccgtctac gcgaagaagt ggcggaacaa atcaaagctt tgaaggacat
     1261 caaggttatg ggcgaatatt acggactcga cctcagccat ccggctacat cagctcaaga
     1321 ggccgtgcaa tgggtataca tggcatatct tgctgccatc aaagagcaag acggtgctgc
     1381 catgtcattg ggtaacgtat cttcgttcct cgatatattc attgaatacg atctggctca
     1441 cggaaaaatc aatgagacgt ttgcgcaaga actgatagac cagttcgtca tcaagctgcg
     1501 catggtacgc catctgcgta tgcagtcgta caatgatatc tttgccggtg acccgacttg
     1561 ggtgaccgaa gccatcggag gacgcttcaa tgacggacgc gtgaaagtca ccaagacttc
     1621 tttccgcttc ttgcagacgc tatacaacct cggtccttct ccggaaccaa acttgaccgt
     1681 actgtggagt cccgaactgc cggaaggctt caaggatttc tgcgccaaag tttccgtaga
     1741 taccagctcc atccagtacg aaaacgacaa cctgatgcgc gaagtgcgca actgcgacga
     1801 ttacggcatt gcctgctgtg tatcctacca agccatcggc aagcaaatcc agttcttcgg
     1861 cgcacgtgcc aaccttgcca aggccctgct gcttgccatc aacggtggcc gttgcgaaaa
     1921 caccggaact gtcatggtga aaggtattcc cgtattgacc ggcgagacac tgaagtttga
     1981 ggaagttatg gccaactaca agaaggtact gactgaaata gcccgtgtct acaacgaggc
     2041 catgaatatc atccactaca tgcatgataa gtattattat gagaaggccc agatggcatt
     2101 catcgatacc aacccacgca tcaatctggc atacggtgtt gccggccttt ccatcgccat
     2161 agactcactg tccgccatca aatatgccaa agtcactgca cgccgcaacg aaatcggcct
     2221 gaccgaaggt ttcgacactg aaggctcatt cccctgcttc ggcaataatg acgaccgtgt
     2281 ggaccacctg ggagtagacc tcgtttacta tttcagcgaa gaactgaaaa agctgcctgt
     2341 ctacaagaat gcccgtccca ccctttcctt gctgactatc acctccaacg tgatgtacgg
     2401 caagaagacc ggtgccactc cggacggacg cgccaaaggt gttgcctttg ctccgggtgc
     2461 caacccaatg cacggacgtg acaagaacgg tgccatcgct tccttgagct ccgtagccaa
     2521 attgcgctac cgcgactcgc aggacggcat cagcaatacg ttctccattg ttcctaaatc
     2581 actgggcccg acaccggaag aacgtgtaga gaacctggta accatgatgg acggctactt
     2641 caccaaaggt gcccaccatt tgaacgtaaa cgtactgaac cgcgaaatgc tggaagatgc
     2701 catggaacat cctgaaaagt atccgcaact caccatccgc gtttccggct atgccgtgaa
     2761 cttcgtgaag ttgagccgcg agcatcagtt ggaagtcatc agccgttcat tccacgaaag
     2821 aatgtaatat gataaatgta cactcctacg aaagtatggg aacattcgac gggccgggtc
     2881 tccggctcgt cgttttcctg caaggatgtc cgttccgttg tctgtattgt gccaatccgg
     2941 atacgattga tgtaaaagga ggcactccca ccactccgga agagattctg caaatggcta
     3001 tcagccaaaa ggcattcttc gggaagaaag gaggtattac tttctccggc ggtgagccta
     3061 ccctgcaagc agaagcgctg attcctcttt tcaaggagct gaaggcgaac ggcatccata
     3121 tctgtctgga cagtaacgga ggcatctgga atgaaaaggt ggaagagctc tttagcctga
     3181 ctgacctcgt gctgctggac atcaagcaat tcaatcccga acgccaccgt acgctgacgg
     3241 gacgaagcaa cgaacagaca ctccgcaccg ctgcctggct cgaagaacat gaacacccct
     3301 tctggctgcg ttatgtactg gttcccggtt acagcgattc ggaagaagac atccgcctgc
     3361 tgggtgaaag tctcggcaaa taccggcaga tacaacgggt ggaaatactt ccttaccacc
     3421 gtctgggcgt tcacaaatac gaagtcatgg gatgggatta caaattgaaa gaagtgaagg
     3481 aaaatactcc ggaacaactg gaacgggcag aaaaactgtt caaagagtat ttcacgaatg
     3541 tgataaccaa ctaaaacaga aaagcaaggc tgtccggata tgattccaga cagccttgct
     3601 tttcatttat aggacaagaa tcctattctt tcaagttata ggtaatctgc tgtacatcat
     3661 cactactgcc acccaccata aagacgaaat caccaggttc taccatataa ttcatctcat
     3721 tgtcatagaa tcccagtgca tcgtcagtta tctgaaaagc tacagttttg ctttcacccg
     3781 cattcagatg aattttcctg aagcccttca actcctttat cgggcgcgta acagacgcta
     3841 ccttatcctg cacatacaac tggactactt cctctcctgc acacttaccg gtattagtca
     3901 cttttaccga cacctccgta tggacgcctg catccttgcc cggcagcagt ttcaaatcag
     3961 aatatccaaa atccgtatac gaaagcccat atccgaaagg ataggcaggc tcagtggaag
     4021 catcgatata ggatgcctta taagtctgtt gcaatgcggt aggacgaccg gtactcttat
     4081 tctggtaata aatgggaatc tgccctacgt gtgcaggaaa tgtcatgggc aatttagccg
     4141 aaggattata ctctccccac aacacattgc atatggcatt gccggcctca ctccccagcc
     4201 accaagtaca cagtaccgca tcagcatgtt cccttatctc attgaagatt accggacgcc
     4261 cacacatgac aagagcaatg acaggttttc cagtctttgc cagttccgac accagcttct
     4321 gctgcccctc gggtatggct atattcactt tggaagcctt ctcgccactc tgaccgaatc
     4381 tctcgcctac cgccacaaca atgacatccg aagcctcagc agcctgcaat gcttcctgca
     4441 gtccggtcaa ttgattgctc ttcatatcat agccttcagc atagttcacc tccagtcctt
     4501 tccggaccag tgcatcatac aaggtcacca ctttgctccg gtccgtagtg gcgg
//
