LOCUS       c00370_gnlPro..         3589 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|CMBKEKEM_370.
ACCESSION   c00370_gnlPro..
VERSION     c00370_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-05 15:37:01
            Original ID  :: gnl|Prokka|CMBKEKEM_370
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 3589
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     CDS             complement(1..663)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 75.8;
                     E-value: 1.3e-23)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg370_1"
                     /sec_met_domain="Radical_SAM (E-value: 2.8e-32, bitscore:
                     99.5, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MINVHSYESMGTFDGPGLRLVVFLQGCPFRCLYCANPDTIDVKGG
                     TPTTPEEILQMAISQKAFFGKKGGITFSGGEPTLQAEALIPLFKELKANGIHICLDSNG
                     GIWNEKVEELFSLTDLVLLDIKQFNPERHRTLTGRSNEQTLRTAAWLEEHEHPFWLRYV
                     LVPGYSDSEEDIRLLGESLGKYRQIQRVEILPYHRLGVHKYEVMGWDYKLKEVKENPP"
     protocluster    1..3589
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[0:2893]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      1..2893
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..3589
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..3589
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             complement(665..2893)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate1"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg370_2"
                     /sec_met_domain="Gly_radical (E-value: 1.1e-47, bitscore:
                     148.2, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate1 (E-value: 0.0, bitscore:
                     1171.1, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MELNKTFTNGLWNKEINVSDFVSKNIAPYTGDASFLQGPTERTKR
                     IWDICLKALEEERNNNGVRSLDNTTVSTITSHKAGYIDKDNELIVGLQTDELLKRSIKP
                     FGGINVVAKACRENGVEVDDKVKDIFTHYRKTHNDGVFDVYTEEIRSFRSLGFLTGLPD
                     NYARGRIIGDYRRLALYGIDRLIEAKQEDLRNLTGPMTEARIRLREEVAEQIKALKDIK
                     VMGEYYGLDLSHPATSAQEAVQWVYMAYLAAIKEQDGAAMSLGNVSSFLDIFIEYDLAH
                     GKINETFAQELIDQFVIKLRMVRHLRMQSYNDIFAGDPTWVTEAIGGRFNDGRVKVTKT
                     SFRFLQTLYNLGPSPEPNLTVLWSPELPEGFKDFCAKVSVDTSSIQYENDNLMREVRNC
                     DDYGIACCVSYQAIGKQIQFFGARANLAKALLLAINGGRCENTGTVMVKGIPVLTGETL
                     KFEEVMANYKKVLTEIARVYNEAMNIIHYMHDKYYYEKAQMAFIDTNPRINLAYGVAGL
                     SIAIDSLSAIKYAKVTARRNEIGLTEGFDTEGSFPCFGNNDDRVDHLGVDLVYYFSEEL
                     KKLPVYKNARPTLSLLTITSNVMYGKKTGATPDGRAKGVAFAPGANPMHGRDKNGAIAS
                     LSSVAKLRYRDSQDGISNTFSIVPKSLGPTPEERVENLVTMMDGYFTKGAHHLNVNVLN
                     REMLEDAMEHPEKYPQLTIRVSGYAVNFVKLSREHQLEVISRSFHERM"
ORIGIN
        1 cggaggattt tccttcactt ctttcaattt gtaatcccat cccatgactt cgtatttgtg
       61 aacgcccaga cggtggtaag gaagtatttc cacccgttgt atctgccggt atttgccgag
      121 actttcaccc agcaggcgga tgtcttcttc cgaatcgctg taaccgggaa ccagtacata
      181 acgcagccag aaggggtgtt catgttcttc gagccaggca gcggtgcgga gtgtctgttc
      241 gttgcttcgt cccgtcagcg tacggtggcg ttcgggattg aattgcttga tgtccagcag
      301 cacgaggtca gtcaggctaa agagctcttc caccttttca ttccagatgc ctccgttact
      361 gtccagacag atatggatgc cgttcgcctt cagctccttg aaaagaggaa tcagcgcttc
      421 tgcttgcagg gtaggctcac cgccggagaa agtaatacct cctttcttcc cgaagaatgc
      481 cttttggctg atagccattt gcagaatctc ttccggagtg gtgggagtgc ctccttttac
      541 atcaatcgta tccggattgg cacaatacag acaacggaac ggacatcctt gcaggaaaac
      601 gacgagccgg agacccggcc cgtcgaatgt tcccatactt tcgtaggagt gtacatttat
      661 catattacat tctttcgtgg aatgaacggc tgatgacttc caactgatgc tcgcggctca
      721 acttcacgaa gttcacggca tagccggaaa cgcggatggt gagttgcgga tacttttcag
      781 gatgttccat ggcatcttcc agcatttcgc ggttcagtac gtttacgttc aaatggtggg
      841 cacctttggt gaagtagccg tccatcatgg ttaccaggtt ctctacacgt tcttccggtg
      901 tcgggcccag tgatttagga acaatggaga acgtattgct gatgccgtcc tgcgagtcgc
      961 ggtagcgcaa tttggctacg gagctcaagg aagcgatggc accgttcttg tcacgtccgt
     1021 gcattgggtt ggcacccgga gcaaaggcaa cacctttggc gcgtccgtcc ggagtggcac
     1081 cggtcttctt gccgtacatc acgttggagg tgatagtcag caaggaaagg gtgggacggg
     1141 cattcttgta gacaggcagc tttttcagtt cttcgctgaa atagtaaacg aggtctactc
     1201 ccaggtggtc cacacggtcg tcattattgc cgaagcaggg gaatgagcct tcagtgtcga
     1261 aaccttcggt caggccgatt tcgttgcggc gtgcagtgac tttggcatat ttgatggcgg
     1321 acagtgagtc tatggcgatg gaaaggccgg caacaccgta tgccagattg atgcgtgggt
     1381 tggtatcgat gaatgccatc tgggccttct cataataata cttatcatgc atgtagtgga
     1441 tgatattcat ggcctcgttg tagacacggg ctatttcagt cagtaccttc ttgtagttgg
     1501 ccataacttc ctcaaacttc agtgtctcgc cggtcaatac gggaatacct ttcaccatga
     1561 cagttccggt gttttcgcaa cggccaccgt tgatggcaag cagcagggcc ttggcaaggt
     1621 tggcacgtgc gccgaagaac tggatttgct tgccgatggc ttggtaggat acacagcagg
     1681 caatgccgta atcgtcgcag ttgcgcactt cgcgcatcag gttgtcgttt tcgtactgga
     1741 tggagctggt atctacggaa actttggcgc agaaatcctt gaagccttcc ggcagttcgg
     1801 gactccacag tacggtcaag tttggttccg gagaaggacc gaggttgtat agcgtctgca
     1861 agaagcggaa agaagtcttg gtgactttca cgcgtccgtc attgaagcgt cctccgatgg
     1921 cttcggtcac ccaagtcggg tcaccggcaa agatatcatt gtacgactgc atacgcagat
     1981 ggcgtaccat gcgcagcttg atgacgaact ggtctatcag ttcttgcgca aacgtctcat
     2041 tgatttttcc gtgagccaga tcgtattcaa tgaatatatc gaggaacgaa gatacgttac
     2101 ccaatgacat ggcagcaccg tcttgctctt tgatggcagc aagatatgcc atgtataccc
     2161 attgcacggc ctcttgagct gatgtagccg gatggctgag gtcgagtccg taatattcgc
     2221 ccataacctt gatgtccttc aaagctttga tttgttccgc cacttcttcg cgtagacgga
     2281 tgcgtgcttc ggtcatcggg ccggtgaggt tgcgcaagtc ctcctgcttg gcttcaatca
     2341 gacggtcgat gccgtagaga gccaatcggc ggtagtcgcc gatgatacgt ccgcgggcat
     2401 agttgtcggg caatccggta aggaatccca gtgagcggaa ggaacggatt tcttcggtat
     2461 atacatcgaa tacgccgtcg ttatgtgttt tgcggtaatg ggtgaagatg tcttttactt
     2521 tatcgtccac ttccactccg ttttcacggc atgcttttgc taccacgttg atgcctccga
     2581 aaggtttgat ggatcgtttc aacagttcat cagtctgcaa accgacaata agttcattgt
     2641 ccttatcaat atacccggct ttatgagaag tgatggtaga tacggtggta ttgtccagtg
     2701 aacggacgcc attgttgttc ctttcttctt cgagagcctt cagacagatg tcccagatac
     2761 gtttggtacg ttcggtaggt ccctgcaaga aagatgcatc cccggtatag ggtgcgatgt
     2821 ttttactgac gaaatcactg acattgattt ctttattcca aagcccattg gtaaaggttt
     2881 tgttcaattc cataaaattt gtttttgtta gtaggttaga taatgttatc ctttagagcg
     2941 cacaaaaata ctgaataaat tccgtaattt tataagagat acttctcttt ttatgttgaa
     3001 cagcataaaa taactattat taggtagtga atgtccgggg gagtagagat gttaatactt
     3061 tctatatgtg gtttatgaat tgaaacaaga accgaataat aatctgcatt atttacaaat
     3121 gatgtaggtt taaggaacag tcgtattctg aacctaattc agtcgtatag tgaatgccat
     3181 tcagtcgtat actgaatagg gttcaatcgt atactgaatg tggttctttg taaatatact
     3241 gatagccagt aagttataaa actctgtctt gcccttgata aatttcaaca aatagcctaa
     3301 atgccgttat gttcgttccg tagtgggtat aaacaagaat tttggtataa ttgctctgtt
     3361 tttgtgcaga aaagtttgta gaataaaaaa tgatttgtat ctttgcaccg ctttaaaaga
     3421 aaagcacttc tgaaaaagaa gttttggaga ggtggcagag tggtcgattg cggcggtctt
     3481 gaaaaccgtt gtgctgcgag gcacccgggg ttcgaatccc tgtctctccg ctgaaaagaa
     3541 ttgcaaaaca aagaaaatcc ctgtaaatta atactttacg gggattttt
//
