LOCUS       c00220_gnlPro..         6413 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|ELBKKIIG_220.
ACCESSION   c00220_gnlPro..
VERSION     c00220_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-05 16:11:27
            Original ID  :: gnl|Prokka|ELBKKIIG_220
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 6413
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     CDS             complement(1..690)
                     /locus_tag="ctg220_1"
                     /transl_table=11
                     /translation="MRFTRLILALTSFLILITQTDLFASTVLHKTSMVDLSNNAIICMH
                     QDPDGYLWIGTYDGLNLYNGKDTYVYRFELNNKNSLCSNIIHKISDAEPGFLWISTSLG
                     INKFSLKERKVTESFPGYMECDLLASDSSGITLAICKENRISCYTPSNDGFRDLPREGV
                     GPQTVRTLFAGTDGHFYLLLASGILKKLTPDFSDTTFNLLSEERQFHNKPILSAFYENN
                     HIFIVDTD"
     protocluster    1..6413
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[3320:6296]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      3321..6296
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..6413
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..6413
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             847..3150
                     /locus_tag="ctg220_2"
                     /transl_table=11
                     /translation="MEQCEENSQENDDVIIEKEISKLIEEQRLFTESIYACLPVGIEIY
                     DAHGVLRSINDHALRMYGVDDRTSVVNIVNLYKSPFVDSELLARIQSGEDISLEFEYDF
                     DRVNKDAYYSSHNKDTMIYGVKVIPIWSRKKHLIGHILLANDMTSVKDVEFRTEESKKN
                     LEMAMKAANMASWVYDVNKKVFNPLYGLTVAKKNMPMEAVLDILHPQDRAPLIRLFSQL
                     IGNEIEQGQMTLRFYNEQEEMYRYYESRMRLSTEHRGKLQIIGTQMDITERLQMAKKAQ
                     DLIAKRDLAMKVSNIVHWDFDVNSQKFESYNDPINDYVSDRLLTVSEYMDVIYPEDRSV
                     FYDAMQSLIAGKNVTINFTCRIQTKYDETWQYCDFMGVPFDQDENGDIIRYTGFRQNIP
                     KIQQLNRELKERNYKIELSFKTVGMSYWDFDVKSLKFSAFNDPINDFYSEKTITPEDYL
                     NATHPDDVVFVRKYMEHMFRGIDKDFNVKYRSKTKWDDEWQTLLVTGIQVERDKEGHVT
                     RYTGITINNTKWEKMIQQLKELKEKAELSDRLKSAFLANMSHEIRTPLNAIVGFSELMV
                     TCDDPEEKKEYINIIQSNNELLLRLINDILDLSKIESGIIERRKENFNLAKVCNELFVT
                     IQAKMTNPNVELRLDGPNSECWVLLDRNRLKQVWMNFLTNAVKCTKSGYIKMGYGIERE
                     GLRIYVEDTGIGIPDDLHDKVFTRFEKLNEFSQGTGLGLTISRAIVEAAGGEVGFTSKL
                     GVGSTFWAWLPLRP"
     CDS             3321..5561
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate2"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg220_3"
                     /sec_met_domain="Gly_radical (E-value: 1.6e-46, bitscore:
                     145.5, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate2 (E-value: 1.2e-282, bitscore:
                     926.7, seeds: 33, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKFENECWDHFKGETWKREINVRDFIQSNYTPYEGDDSFLVASSE
                     KTRKVWNKLTEMFKVEREKGVYDAETKLPQGIDVYGPGYIDKENEVIVGLQTDAPLKRG
                     IFPKGGIRMVENSLEAYGYHLDPMTKEIFTKYRKTHNEGVFSAYTEEMIAARRSAIITG
                     LPDAYGRGRIIGDYRRVALYGTAILIEEKKRFLNRLDIQEITEEIIQSREEISEQIKAL
                     KAFEKMCASYGFDVTRPAQNAREAVQFVYLAYLAAVKDQDGAAMSIGRTSTFLDIYIEK
                     DIREGKLTEEEAQELVDQLIIKLRIVRFLRTPEYNDLFSGDPVWVTESLGGQGVDGRSL
                     VTRTSYRYLHTLYNLGPAPEPNLTVLWFKNAPENWKRFCAKVSIDTSAIQYENDDLMRP
                     DYGDDYGIACCVSPMKIGKQMQFFGARANLAKCLLYAINGGRDERSGVQVAPMFEPVRG
                     EYLEYDEVMAKYEQMMRWLAKVYVNALKIIHYMHDKYAYEAFEMSLHDGDVERIRATGI
                     AGLSIVADSLAAIRDTKVRVIRDERGLAVDFEREGEYVPFGNNDDRTDSIAVEITEKFM
                     EYLRQHETYRHAKPTQSILTITSNVVYGKKTGTTPDGRIGGTPFAPGANPMNGRDTKGA
                     IAALASVAKLPFQHAHDGISYTFAVSPATLGKEREVQINNLVSLLDGYFTPDGGQHLNV
                     NVFDKDLLIDAMEHPEKYPQLTIRVSGYAVNFVKLTREQQLDVISRTINHSL"
     CDS             5562..6296
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 67.6;
                     E-value: 4.7e-21)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg220_4"
                     /sec_met_domain="Radical_SAM (E-value: 5.1e-34, bitscore:
                     106.2, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="VIKGKIHSLESFGTVDGPGIRFVVFMQGCPLRCLYCHNPDTWNPK
                     GKVKYQMTPGELLTEVLRYKSFIARGGVTVTGGEPLLQPEFLKEFFRLCQEQGLHTALD
                     TSGFVCTSKAWEVLDYADLVLLDIKTLNPDLHPLLAGVKQDNTLLFLDELERRGIDTWI
                     RHVIVPGYTDNDEWLEALARYVSSYKVVRKVELLPYHTMGTYKYEQLGLDYPLKGVEPL
                     SKERLDNAKAIFSRYKPENNKA"
ORIGIN
        1 gtcggtatct actatgaaaa tatgattatt ctcgtaaaat gcggataaga tcggtttatt
       61 atggaattgg cgttcttccg atagcaaatt aaaagtagta tcggagaaat ccggagtcaa
      121 ctttttcagt atgcccgagg ctaataataa ataaaaatgt ccgtccgtcc cggcgaataa
      181 ggtacgtacg gtttgagggc ctactccttc tctcggcagg tcccggaacc catcgttaga
      241 tggggtgtag caagagatcc tgttttcctt acaaatagcg agcgtgatgc cggatgaatc
      301 ggacgctaat agatcgcatt ccatgtatcc ggggaaagac tcggtgactt tccgttcttt
      361 tagtgagaat ttattgatac ctaaagaggt tgatatccat aaaaagccgg gttctgcgtc
      421 ggatattttg tgaatgatat tactacaaag agagtttttg ttatttaatt cgaaacgata
      481 tacatacgta tcttttccgt tgtataagtt aagtccgtcg taagtcccga tccacaaata
      541 gccatccgga tcttgatgca tgcaaataat agcgttattt gagagatcaa ccatagaagt
      601 tttatgcaat acggttgagg caaataggtc tgtttgcgtg atcaatataa ggaaactggt
      661 taacgctaga ataagccttg tgaatctcat gtatttaaga tttagtgttt gttttcagcg
      721 caaagataat atctttatga acgaacagaa aagaattcac ttttttgttt acttttgcaa
      781 aaagcagggc tttcgtataa attagaatgt ctgacgtatg ataatacgaa ataaagtaag
      841 tgacagatgg aacaatgtga agagaattct caagaaaatg acgatgtgat aatagagaaa
      901 gaaatttcta aattaataga agaacagcgt ttatttacgg agagtatata tgcttgtctg
      961 ccggtgggta ttgaaattta tgacgcacat ggtgtgctgc gtagtataaa cgaccatgcg
     1021 ttgaggatgt atggtgtgga cgaccggaca tcggtggtga atatagtcaa tctttacaaa
     1081 agtccatttg ttgattcaga acttttagct cggatacaat cgggtgaaga tatttccttg
     1141 gagtttgagt atgattttga tcgggttaat aaggatgcat attattcttc ccataataaa
     1201 gacacgatga tctatggggt taaagttatc ccgatatgga gtagaaaaaa acatcttatc
     1261 ggacatatat tactcgcaaa tgatatgact tccgtgaaag atgtggaatt ccgtacggaa
     1321 gaaagcaaga aaaatctgga gatggccatg aaagcggcta atatggcttc ttgggtatat
     1381 gatgtgaata aaaaggtttt taatccatta tatggactaa ccgtagccaa aaaaaatatg
     1441 ccaatggagg cggtactgga tattttgcat ccccaagatc gagctccgtt aatccggctc
     1501 ttctctcaat tgataggaaa tgagatagaa caaggacaaa tgactttgcg tttctataac
     1561 gagcaagaag agatgtatcg ttattatgag agccggatgc gcctatcgac agaacatcgt
     1621 ggaaaattgc agatcatcgg gacacaaatg gatataaccg aaaggttgca aatggccaag
     1681 aaagcgcagg atctgatcgc aaaacgtgat ctggccatga aagtcagtaa tattgttcat
     1741 tgggattttg atgtaaattc ccagaaattc gaatcttata atgatccgat caacgattac
     1801 gtttcagatc ggttattgac cgtttcggag tatatggatg ttatataccc cgaggaccgg
     1861 tctgtttttt acgacgcaat gcaatccctg atagctggaa agaatgtcac catcaatttt
     1921 acctgtcgca tacaaaccaa gtatgatgag acttggcagt attgtgattt tatgggtgtc
     1981 cctttcgatc aagatgaaaa tggagatata atacgctata cgggattcag gcaaaacatt
     2041 cctaagatcc agcaattgaa tcgggaactt aaagaaagaa attacaagat agagctttct
     2101 ttcaagacgg ttggtatgtc gtattgggat tttgatgtaa aaagcctgaa attcagtgct
     2161 tttaatgatc ccatcaatga tttttattcg gaaaagacaa tcactcccga ggattatttg
     2221 aacgctacac atccggatga tgtggttttt gtgcggaaat atatggagca tatgttccgg
     2281 ggcatagata aagatttcaa cgttaaatat cgttccaaaa ccaaatggga tgacgagtgg
     2341 cagaccttat tggttactgg aatccaggtt gaaagagata aagaaggcca tgtgactcgc
     2401 tataccggta ttacgatcaa taatacgaag tgggagaaga tgattcagca attgaaggaa
     2461 ttaaaggaga aagcggaact ttcagatcgt ctcaagtctg cgtttctcgc taatatgagt
     2521 cacgagatac gtaccccgtt aaacgcgatt gttggttttt cagagttaat ggtaacttgt
     2581 gatgatccgg aagaaaaaaa agaatatata aatatcatcc aatccaataa tgagttgtta
     2641 ttgcgattga tcaacgatat ccttgatctt tccaagatag agtcaggcat aattgaacgt
     2701 aggaaggaga atttcaatct ggccaaggtt tgtaatgagt tatttgttac gattcaggcg
     2761 aagatgacaa atccgaatgt cgagttacgt ttagatggtc ccaactcgga gtgctgggta
     2821 cttctggata gaaaccggct taagcaagta tggatgaatt tcctcactaa tgcggtaaaa
     2881 tgcaccaaat ccggatatat taaaatggga tatggcattg aaagggaagg tcttcgtatc
     2941 tatgttgagg atacgggaat cggtataccg gatgatttgc atgacaaagt atttacccgt
     3001 tttgagaaat tgaatgagtt ctcgcagggt accggacttg gcttgacgat ctccagagct
     3061 atcgtagaag cggcaggggg agaagtgggc ttcacttcaa aacttggtgt cggatctact
     3121 ttttgggcat ggttgcctct tcgcccttag gtctgactcc ccatttattg gtaatgtcct
     3181 tttaaaatag tgaaggatga gaagcttttt tatcgcaatt tatagggatt ttagaactgg
     3241 tgtagcatat gtgacagaaa tatgacgata attctcctac ctttgcggcg gtaaaaatgt
     3301 ggtattagat aagattgttt atgaaatttg agaatgagtg ctgggatcac tttaagggtg
     3361 agacctggaa aagagagatc aatgtaagag attttatcca gagtaactat acgccttatg
     3421 aaggggatga ttccttttta gtcgcttctt ccgagaagac ccgtaaggtc tggaacaagc
     3481 tgaccgagat gtttaaggtc gagagggaaa agggagttta tgacgccgag acaaaattac
     3541 ctcaagggat cgatgtatat ggcccgggct atattgataa ggagaatgag gtgatcgttg
     3601 ggttacaaac cgatgctccg ctgaaacgtg gtatcttccc gaaaggcggt atccgtatgg
     3661 tcgagaatag cttggaagct tatggttatc atttggaccc gatgacgaaa gagatcttca
     3721 cgaagtaccg taagacgcat aacgagggcg ttttctccgc atatacggaa gagatgattg
     3781 ccgcacgtcg ttcggctatt atcaccggtc tgcccgacgc gtatggtaga ggacgtatta
     3841 ttggtgacta tcgtcgtgtc gctctgtatg gtacggcaat cttgatcgag gagaaaaagc
     3901 gtttcttgaa tcgtttggat atccaagaga tcacggaaga gatcatccag agccgtgagg
     3961 agatttccga acagatcaag gctttgaaag cgttcgagaa aatgtgcgct agctatggct
     4021 ttgatgtgac tcgtccggca caaaacgctc gtgaggcggt tcagtttgtg tatttagctt
     4081 atctggcggc ggtaaaagac caagatggcg cggcgatgtc tatcggacgt acttctacgt
     4141 ttttggatat ttatatcgag aaagacatcc gtgagggtaa gttgaccgaa gaggaggcgc
     4201 aagaattggt agaccagctg atcatcaagt tgcgtatcgt tcgtttctta cgtacgccgg
     4261 aatacaatga tttgttctcc ggtgatccgg tttgggtaac agagtccttg ggcggccaag
     4321 gagtggatgg ccgttctttg gtaacaagaa cttcctatcg ttatctccat acattatata
     4381 atctaggtcc ggcaccagaa cccaatttga cggttctgtg gtttaagaac gctcccgaga
     4441 actggaaacg tttctgtgcc aaggtctcta tcgatacgtc ggctatccaa tatgagaacg
     4501 acgatttgat gcgtccggat tatggagatg attatggtat cgcttgttgc gtttctccta
     4561 tgaagatcgg taagcagatg cagttcttcg gagcccgtgc gaatttggcg aaatgtttat
     4621 tatatgcgat caacggagga cgggatgagc gcagcggcgt acaggtagcc ccgatgttcg
     4681 agccggttcg cggcgagtat ctggaatacg atgaggtgat ggctaagtat gagcaaatga
     4741 tgcgctggtt ggcgaaagtg tatgtgaacg ccttgaagat aatccattat atgcatgata
     4801 aatacgcgta tgaggcattc gagatgtcgc ttcacgatgg ggatgtagag cgtatccgtg
     4861 cgacaggtat cgccggtctt tctatcgttg ccgattcgtt ggctgctatc cgtgatacga
     4921 aagtacgtgt gatccgtgac gagcgtggct tggcggttga tttcgaacgt gagggcgaat
     4981 atgttccttt cggtaataac gatgatcgta cggatagtat cgctgtagag atcaccgaga
     5041 agttcatgga atatttgcgt cagcacgaga cctatcgcca tgcgaaacct acgcagtcca
     5101 tccttacgat cacctctaat gtggtatatg gtaagaagac tggtacgaca ccggatggcc
     5161 gtatcggtgg aactcctttc gctccgggtg cgaaccctat gaacggtcgt gatacgaaag
     5221 gtgctatcgc tgcgctagct tcggtagcga agcttccatt ccagcatgcg catgacggca
     5281 tctcttatac tttcgctgtt tctccggcta cgttgggaaa ggagcgtgaa gtgcagatca
     5341 ataatctggt tagcttattg gatggctatt ttactccgga tggaggtcag catctgaatg
     5401 ttaacgtatt tgataaggat ttgttgatcg acgctatgga gcatcccgag aaatatccgc
     5461 aacttacgat ccgtgtatcc ggatatgcgg taaacttcgt gaaactgact cgtgaacaac
     5521 agttggatgt tatttcaaga accattaacc attcactgta agtgataaaa ggtaagatac
     5581 attcattaga gagtttcggg accgtagatg gtcccggaat tcgctttgtc gtttttatgc
     5641 agggctgccc gttgcgttgc ctgtattgcc ataacccgga tacttggaac ccgaaaggga
     5701 aggtgaaata tcagatgact cccggcgagc tattgacgga agtcttacgg tataagagtt
     5761 ttatcgcccg tgggggggtg actgttaccg gtggggaacc tttattgcaa cctgagtttc
     5821 tgaaagagtt tttccgtttg tgccaagagc aaggtcttca cacagctttg gacacatctg
     5881 gtttcgtatg tacctcaaaa gcttgggaag tcttggatta tgcggactta gtgttattgg
     5941 acatcaaaac cttaaacccg gatttacatc cgttactggc gggtgtcaag caggataata
     6001 cgcttctgtt cttggatgaa ttggagagac gtggcataga tacttggatt cgccatgtta
     6061 ttgtccccgg atatacggat aatgacgagt ggctggaggc gttagctcgg tatgtcagtt
     6121 catataaagt ggttcgcaaa gtggaattat tgccttatca tacgatggga acctataaat
     6181 atgagcaatt aggcctcgat tatccgttaa agggggtgga acccttatca aaagaacgtt
     6241 tagataatgc gaaagcgata ttctctcgct acaaaccaga aaataacaag gcctgatctc
     6301 tcgatcaagc cttgcgatta tgcaacagct aatcagggaa aacaatctaa tccgattagc
     6361 ttttaaacga tagtaataat tttttgccta gactaacctt gactatttaa tat
//
