LOCUS       c00099_gnlPro..         4639 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|NPGOMCPD_99.
ACCESSION   c00099_gnlPro..
VERSION     c00099_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-05 15:44:56
            Original ID  :: gnl|Prokka|NPGOMCPD_99
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 4639
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..4639
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[684:3640]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      685..3640
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..4639
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..4639
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             685..2913
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate1"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg99_1"
                     /sec_met_domain="Gly_radical (E-value: 1.7e-47, bitscore:
                     148.2, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate1 (E-value: 0.0, bitscore:
                     1171.1, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MELNKTFTNGLWNKEINVSDFVSKNIAPYTGDASFLQGPTERTKR
                     IWDICLKALEEERNNNGVRSLDNTTVSTITSHKAGYIDKDNELIVGLQTDELLKRSIKP
                     FGGINVVAKACRENGVEVDDKVKDIFTHYRKTHNDGVFDVYTEEIRSFRSLGFLTGLPD
                     NYARGRIIGDYRRLALYGIDRLIEAKQEDLRNLTGPMTEARIRLREEVAEQIKALKDIK
                     VMGEYYGLDLSHPATSAQEAVQWVYMAYLAAIKEQDGAAMSLGNVSSFLDIFIEYDLAH
                     GKINETFAQELIDQFVIKLRMVRHLRMQSYNDIFAGDPTWVTEAIGGRFNDGRVKVTKT
                     SFRFLQTLYNLGPSPEPNLTVLWSPELPEGFKDFCAKVSVDTSSIQYENDNLMREVRNC
                     DDYGIACCVSYQAIGKQIQFFGARANLAKALLLAINGGRCENTGTVMVKGIPVLTGETL
                     KFEEVMANYKKVLTEIARVYNEAMNIIHYMHDKYYYEKAQMAFIDTNPRINLAYGVAGL
                     SIAIDSLSAIKYAKVTARRNEIGLTEGFDTEGSFPCFGNNDDRVDHLGVDLVYYFSEEL
                     KKLPVYKNARPTLSLLTITSNVMYGKKTGATPDGRAKGVAFAPGANPMHGRDKNGAIAS
                     LSSVAKLRYRDSQDGISNTFSIVPKSLGPTPEERVENLVTMMDGYFTKGAHHLNVNVLN
                     REMLEDAMEHPEKYPQLTIRVSGYAVNFVKLSREHQLEVISRSFHERM"
     CDS             2915..3640
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 75.5;
                     E-value: 1.7e-23)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg99_2"
                     /sec_met_domain="Radical_SAM (E-value: 5.5e-32, bitscore:
                     99.2, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MINVHSYESMGTFDGPGLRLVVFLQGCPFRCLYCANPDTIDVKGG
                     TPTTPEEILQMAISQKAFFGKKGGITFSGGEPTLQAEALIPLFKELKANGIHICLDSNG
                     GIWNEKVEELFSLTDLVLLDIKQFNPERHRTLTGRSNEQTLRTAAWLEEHEHPFWLRYV
                     LVPGYSDSEEDIRLLGESLGKYRQIQRVEILPYHRLGVHKYEVMGWDYKLKEVKENTPE
                     QLERAEKLFKEYFTNVITN"
     CDS             complement(3709..4638)
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Fn3-like"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Glyco_hydro_3_C"
                     /gene_functions="other (smcogs) SMCOG1041:Fibronectin type
                     III-like domain (Score: 88.9; E-value: 6.1e-28)"
                     /gene_kind="other"
                     /locus_tag="ctg99_3"
                     /sec_met_domain="Fn3-like (E-value: 2.9e-29, bitscore:
                     88.9, seeds: 729, tool: rule-based-clusters)"
                     /sec_met_domain="Glyco_hydro_3_C (E-value: 3.1e-43,
                     bitscore: 135.8, seeds: 116, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="ATTDRSKVVTLYDALVRKGLEVNYAEGYDMKSNQLTGLQEALQAA
                     EASDVIVVAVGERFGQSGEKASKVNIAIPEGQQKLVSELAKTGKPVIALVMCGRPVIFN
                     EIREHADAVLCTWWLGSEAGNAICNVLWGEYNPSAKLPMTFPAHVGQIPIYYQNKSTGR
                     PTALQQTYKASYIDASTEPAYPFGYGLSYTDFGYSDLKLLPGKDAGVHTEVSVKVTNTG
                     KCAGEEVVQLYVQDKVASVTRPIKELKGFRKIHLNAGESKTVAFQITDDALGFYDNEMN
                     YMVEPGDFVFMVGGSSDDVQQITYNLKE"
ORIGIN
        1 tgtagctttt tgtccgtttg ctgaccattt ctgtccagca cgttcagcgg agagacaggg
       61 attcgaaccc cgggtgcctc gcagcacaac ggttttcaag accgccgcaa tcgaccactc
      121 tgccacctct ccaaaacttc tttttcagaa gtgcttttct tttaaagcgg tgcaaagata
      181 caaatcattt tttattctac aaacttttct gcacaaaaac agagcaatta taccaaaatt
      241 cttgtttata cccactacgg aacgaacata acggcattta ggctatttgt tgaaatttat
      301 caagggcaag acagagtttt ataacttact ggctatcagt atatttacaa agaaccacat
      361 tcagtatacg attgaaccct attcagtata cgactgaatg gcattcacta tacgactgaa
      421 ttaggttcag aatacgactg ttccttaaac ctacatcatt tgtaaataat gcagattatt
      481 attcggttct tgtttcaatt cataaaccac atatagaaag tattaacatc tctactcccc
      541 cggacattca ctacctaata atagttattt tatgctgttc aacataaaaa gagaagtatc
      601 tcttataaaa ttacggaatt tattcagtat ttttgtgcgc tctaaaggat aacattatct
      661 aacctactaa caaaaacaaa ttttatggaa ttgaacaaaa cctttaccaa tgggctttgg
      721 aataaagaaa tcaatgtcag tgatttcgtc agtaaaaaca tcgcacccta taccggggat
      781 gcatctttct tgcagggacc taccgaacgt accaaacgta tctgggacat ctgtctgaag
      841 gctctcgaag aagaaaggaa caacaatggc gtccgttcac tggacaatac caccgtatct
      901 accatcactt ctcataaagc cgggtatatt gataaggaca atgaacttat tgtcggtttg
      961 cagactgatg aactgttgaa acgatccatc aaacctttcg gaggcatcaa cgtggtagca
     1021 aaagcatgcc gtgaaaacgg agtggaagtg gacgataaag taaaagacat cttcacccat
     1081 taccgcaaaa cacataacga cggcgtattc gatgtatata ccgaagaaat ccgttccttc
     1141 cgctcactgg gattccttac cggattgccc gacaactatg cccgcggacg tatcatcggc
     1201 gactaccgcc gattggctct ctacggcatc gaccgtctga ttgaagccaa gcaggaggac
     1261 ttgcgcaacc tcaccggccc gatgaccgaa gcacgcatcc gtctacgcga agaagtggcg
     1321 gaacaaatca aagctttgaa ggacatcaag gttatgggcg aatattacgg actcgacctc
     1381 agccatccgg ctacatcagc tcaagaggcc gtgcaatggg tatacatggc atatcttgct
     1441 gccatcaaag agcaagacgg tgctgccatg tcattgggta acgtatcttc gttcctcgat
     1501 atattcattg aatacgatct ggctcacgga aaaatcaatg agacgtttgc gcaagaactg
     1561 atagaccagt tcgtcatcaa gctgcgcatg gtacgccatc tgcgtatgca gtcgtacaat
     1621 gatatctttg ccggtgaccc gacttgggtg accgaagcca tcggaggacg cttcaatgac
     1681 ggacgcgtga aagtcaccaa gacttctttc cgcttcttgc agacgctata caacctcggt
     1741 ccttctccgg aaccaaactt gaccgtactg tggagtcccg aactgccgga aggcttcaag
     1801 gatttctgcg ccaaagtttc cgtagatacc agctccatcc agtacgaaaa cgacaacctg
     1861 atgcgcgaag tgcgcaactg cgacgattac ggcattgcct gctgtgtatc ctaccaagcc
     1921 atcggcaagc aaatccagtt cttcggcgca cgtgccaacc ttgccaaggc cctgctgctt
     1981 gccatcaacg gtggccgttg cgaaaacacc ggaactgtca tggtgaaagg tattcccgta
     2041 ttgaccggcg agacactgaa gtttgaggaa gttatggcca actacaagaa ggtactgact
     2101 gaaatagccc gtgtctacaa cgaggccatg aatatcatcc actacatgca tgataagtat
     2161 tattatgaga aggcccagat ggcattcatc gataccaacc cacgcatcaa tctggcatac
     2221 ggtgttgccg gcctttccat cgccatagac tcactgtccg ccatcaaata tgccaaagtc
     2281 actgcacgcc gcaacgaaat cggcctgacc gaaggtttcg acactgaagg ctcattcccc
     2341 tgcttcggca ataatgacga ccgtgtggac cacctgggag tagacctcgt ttactatttc
     2401 agcgaagaac tgaaaaagct gcctgtctac aagaatgccc gtcccaccct ttccttgctg
     2461 actatcacct ccaacgtgat gtacggcaag aagaccggtg ccactccgga cggacgcgcc
     2521 aaaggtgttg cctttgctcc gggtgccaac ccaatgcacg gacgtgacaa gaacggtgcc
     2581 atcgcttcct tgagctccgt agccaaattg cgctaccgcg actcgcagga cggcatcagc
     2641 aatacgttct ccattgttcc taaatcactg ggcccgacac cggaagaacg tgtagagaac
     2701 ctggtaacca tgatggacgg ctacttcacc aaaggtgccc accatttgaa cgtaaacgta
     2761 ctgaaccgcg aaatgctgga agatgccatg gaacatcctg aaaagtatcc gcaactcacc
     2821 atccgcgttt ccggctatgc cgtgaacttc gtgaagttga gccgcgagca tcagttggaa
     2881 gtcatcagcc gttcattcca cgaaagaatg taatatgata aatgtacact cctacgaaag
     2941 tatgggaaca ttcgacgggc cgggtctccg gctcgtcgtt ttcctgcaag gatgtccgtt
     3001 ccgttgtctg tattgtgcca atccggatac gattgatgta aaaggaggca ctcccaccac
     3061 tccggaagag attctgcaaa tggctatcag ccaaaaggca ttcttcggga agaaaggagg
     3121 tattactttc tccggcggtg agcctaccct gcaagcagaa gcgctgattc ctcttttcaa
     3181 ggagctgaag gcgaacggca tccatatctg tctggacagt aacggaggca tctggaatga
     3241 aaaggtggaa gagctcttta gcctgactga cctcgtgctg ctggacatca agcaattcaa
     3301 tcccgaacgc caccgtacgc tgacgggacg aagcaacgaa cagacactcc gcaccgctgc
     3361 ctggctcgaa gaacatgaac accccttctg gctgcgttat gtactggttc ccggttacag
     3421 cgattcggaa gaagacatcc gcctgctggg tgaaagtctc ggcaaatacc ggcagataca
     3481 acgggtggaa atacttcctt accaccgtct gggcgttcac aaatacgaag tcatgggatg
     3541 ggattacaaa ttgaaagaag tgaaggaaaa tactccggaa caactggaac gggcagaaaa
     3601 actgttcaaa gagtatttca cgaatgtgat aaccaactaa aacagaaaag caaggctgtc
     3661 cggatatgat tccagacagc cttgcttttc atttatagga caagaatcct attctttcaa
     3721 gttataggta atctgctgta catcatcact actgccaccc accataaaga cgaaatcacc
     3781 aggttctacc atataattca tctcattgtc atagaatccc agtgcatcgt cagttatctg
     3841 aaaagctaca gttttgcttt cacccgcatt cagatgaatt ttcctgaagc ccttcaactc
     3901 ctttatcggg cgcgtaacag acgctacctt atcctgcaca tacaactgga ctacttcctc
     3961 tcctgcacac ttaccggtat tagtcacttt taccgacacc tccgtatgga cgcctgcatc
     4021 cttgcccggc agcagtttca aatcagaata tccaaaatcc gtatacgaaa gcccatatcc
     4081 gaaaggatag gcaggctcag tggaagcatc gatataggat gccttataag tctgttgcaa
     4141 tgcggtagga cgaccggtac tcttattctg gtaataaatg ggaatctgcc ctacgtgtgc
     4201 aggaaatgtc atgggcaatt tagccgaagg attatactct ccccacaaca cattgcatat
     4261 ggcattgccg gcctcactcc ccagccacca agtacacagt accgcatcag catgttccct
     4321 tatctcattg aagattaccg gacgcccaca catgacaaga gcaatgacag gttttccagt
     4381 ctttgccagt tccgacacca gcttctgctg cccctcgggt atggctatat tcactttgga
     4441 agccttctcg ccactctgac cgaatctctc gcctaccgcc acaacaatga catccgaagc
     4501 ctcagcagcc tgcaatgctt cctgcagtcc ggtcaattga ttgctcttca tatcatagcc
     4561 ttcagcatag ttcacctcca gtcctttccg gaccagtgca tcatacaagg tcaccacttt
     4621 gctccggtcc gtagtggcg
//
