LOCUS       c00191_gnlPro..         3732 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|DDKLJDNO_191.
ACCESSION   c00191_gnlPro..
VERSION     c00191_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-07 17:43:23
            Original ID  :: gnl|Prokka|DDKLJDNO_191
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 3732
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..3732
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[423:3487]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      424..3487
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..3732
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..3732
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             424..2691
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate2"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg191_1"
                     /sec_met_domain="Gly_radical (E-value: 8.9e-47, bitscore:
                     145.3, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate2 (E-value: 5.3e-283, bitscore:
                     926.9, seeds: 33, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MISIVKDLKESTALPQEWQGFKPGTWTESIDVRDFIQHNYTPYSG
                     NEEFLSSPSQRTLRLWDELKVLLKREIDNGGVLDADEEVVSSITSHKPGYIDKELEVVV
                     GLQTDAPLKRALMPFGGLRMAQQALESYGFKMCEKTADIFKKIRKTHNEGVFDAYTSDI
                     RAARSAGIITGLPDAYGRGRIIGDYRRVALYGTDKLIAERRKDLKNREHSPLTDELIRL
                     REEMSEQIRALEELAQLGASYGCDLTRPAANAREAVQWTYLGYLAAVKEQNGAAMSLGR
                     VSTFFDIYFTRDLEQGLITEEEVQEIIDQFVMKLRIVRFIRTPDYNNLFSGDPTWVTES
                     IGGMGEDGRTLVTRSSFRMLQTLYNLGPAPEPNLTVLWSRNLPETFKSFCAKVSIETSS
                     VQYENDDLMRPHWGDDYGIACCVSAMRIGKQMQFFGARANLAKCLLYALNGGVDELKGK
                     QVAPPSPRYTEEILNYDEVMALYDKMQDWLAKTYIDALNIIHYMHDKYCYERIEMALHD
                     PEILRTMATGIAGLSVAADSLSAIKYATVKAIRNEEGLIVDFKTEGEFPCYGNNDPRVD
                     DIACSLVSNFMEKLRRLHTYRNSLPTQSILTITSNVVYGKKTGNTPDGRRAGEPFAPGA
                     NPMHGRDRNGAVASMLSVAKLSYDDSLDGISYTFSIVPQALGKEERERRVKLVSLLDAY
                     FAATGHHINVNVLERETLLDAMDHPEKYPQLTIRVSGYAVNFIKLTREQQQEVINRTFH
                     TR"
     CDS             2708..3487
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 53.6;
                     E-value: 9.1e-17)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg191_2"
                     /sec_met_domain="Radical_SAM (E-value: 1.4e-32, bitscore:
                     100.6, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPQVFPQNRDPDGESSVTGLVHSVESCGTVDGPGIRFVLFLSGCS
                     LRCRYCHNPDTSYVRRGRLRSADDVLKEIARYRDFLQAAGGGVTLSGGDPLFQPDFAGA
                     VLKGCRKLGLHTCLDTSGHLGVNAGEEMLADTDLVLLDIKAWNPKRYRALTGGELRPTL
                     EFAERLASLRKPVWLRYVLVPGVTDNLEEIEELSRHAALLGNVERVDVLPFHQLGRFKW
                     DELKLDYTLKDVPEASAELAEQVRGIFRRGGFPDCVH"
ORIGIN
        1 cagcccctga aaggctggaa acggcaaaac tccgcgccgc gtaattatat cgtgaattcc
       61 gggggacaac aaggctaatt ctttgcgaag agaacgcggg tgcgctggct ttggcatgtt
      121 tcacctgtct gtccgttgcg gagcggaaaa tgaggggaac atgcgattgc ggaaaagtga
      181 cggaagatta tttctcttta ctttcctaat ctctattgca gtatctcaac gcttaccgtt
      241 atgaaatctt atgcatgcct ttgtttattg ctggcgggag ttttttgcgt tgcttgttta
      301 aagaattagc cttgttgtcc cccggaattc acgatataat tacgcggcgc ggagttttgc
      361 cgtttccagc ctttcagggg ctgcggaacg tttttctcca gtcttcttca ctacattcta
      421 tttatgatca gcatagtaaa agacttgaag gagtccaccg cccttccgca ggaatggcag
      481 ggattcaagc cgggaacatg gacggagtcc attgacgtac gggatttcat ccagcataac
      541 tacacgccct attccggcaa tgaagagttt ctttccagcc cctcccagcg cacgcttcgt
      601 ttatgggacg agctgaaagt cctgctgaaa cgggaaatag acaacggcgg cgtgctggat
      661 gctgatgaag aagtggtatc ctccatcaca tcccacaagc ccggctacat tgacaaggaa
      721 ctggaagtgg tcgtaggcct ccaaacggac gcccccctga aaagggccct gatgcccttt
      781 ggcggcttgc gcatggccca acaggccctg gaatcctacg ggttcaagat gtgcgagaaa
      841 accgcggaca ttttcaaaaa gatacgcaag acgcacaatg aaggcgtttt cgacgcctac
      901 acttccgata tccgggcggc ccgttccgcc gggattatta ccggccttcc ggacgcctat
      961 ggccgcggcc gcatcatcgg ggactaccgc cgggtggccc tgtacggcac ggataagctg
     1021 attgccgaac gccgcaagga cctcaaaaac cgtgaacatt ccccccttac ggatgagctg
     1081 atacgtctgc gcgaagaaat gagcgaacaa atacgcgccc tggaagaact cgcccagctg
     1141 ggcgcctcct acggctgcga cctcactcgc cccgccgcaa acgcccgcga agccgtgcaa
     1201 tggacgtacc tgggctacct ggctgccgtg aaagaacaga atggagcggc catgtccctg
     1261 ggccgcgttt ccactttctt cgacatttat ttcacccgcg acctggaaca ggggctcatc
     1321 acggaagagg aagttcagga aatcatcgac cagttcgtga tgaagctgcg catcgtccgt
     1381 ttcatccgca cgcctgatta caataacctg ttctccggag accccacctg ggtgacggaa
     1441 tccatcggcg gcatgggaga ggacgggcgc accctcgtca cccgcagttc tttccgcatg
     1501 ctgcagaccc tgtacaacct ggggccggct ccggaaccga acctgaccgt tctgtggtcc
     1561 cggaacctgc cggaaacctt caaaagcttc tgcgccaagg tttccattga aacatcctcc
     1621 gtccaatacg agaacgatga cctgatgcgc ccgcattggg gcgacgacta cggcatcgca
     1681 tgctgcgtgt ccgccatgcg catcggcaag cagatgcagt tcttcggagc gcgcgccaac
     1741 ctcgccaagt gccttctcta cgccctgaac ggcggcgtgg atgaactcaa gggcaagcag
     1801 gtggccccgc cctccccccg ctacacggag gaaattctca actatgatga agtgatggcc
     1861 ctgtacgaca agatgcagga ctggctcgcc aaaacttaca ttgacgcgct gaacatcatc
     1921 cactacatgc acgacaaata ctgctatgaa cgcattgaaa tggcgctgca tgatccggaa
     1981 attctgcgca cgatggctac gggaatcgcc gggctttccg tggcggcgga ctccctgtcc
     2041 gccatcaagt atgccaccgt aaaagccatc cgcaatgaag aagggctgat tgtggacttc
     2101 aagacggaag gggaattccc ctgttacggg aacaatgacc cgcgcgtgga cgacatagca
     2161 tgcagcctgg tcagcaattt catggaaaaa ctgcgcaggc tccacactta ccgcaattcc
     2221 ctgcccaccc agtccatcct gacgatcacc tccaacgtgg tgtacggcaa aaagacgggc
     2281 aacacgccgg acggccgccg agccggggaa ccgttcgccc ccggagccaa ccccatgcac
     2341 ggaagggaca ggaacggagc cgtggcctcc atgctctccg tagccaagct gtcctatgac
     2401 gactccctgg acggcatctc ctacaccttc tccatcgttc cccaggccct gggcaaggag
     2461 gaacgtgaac gccgcgtcaa gctcgtctcc ctgctggacg cctactttgc cgctacaggt
     2521 caccacatta atgtgaacgt actggaacgg gaaacgcttc tcgacgccat ggatcacccg
     2581 gaaaaatacc cgcagcttac catccgcgtt tccggctatg ccgtgaattt catcaagctg
     2641 acccgggaac agcagcagga ggtcatcaac cgcactttcc acacccgtta acccggactg
     2701 caagcggatg ccgcaggttt ttccacaaaa ccgtgatccg gacggagaaa gctccgtcac
     2761 cggtttggtt cattccgtgg aatcctgcgg caccgtggac ggccctggca tccgcttcgt
     2821 ccttttcctg tccggttgca gcctgcgctg ccgctactgc cataatccgg atacctccta
     2881 cgtgcggagg ggccggctca ggagcgcaga tgatgtcctc aaggaaatag cccgttaccg
     2941 ggattttttg caggcggcgg gcggcggcgt caccctctcc ggcggggacc ccctcttcca
     3001 gccggatttt gccggtgccg tgctgaaagg ctgccggaaa ctgggcctgc acacctgcct
     3061 ggacacctcc ggccatctgg gcgtgaatgc gggcgaagaa atgctggcgg atacggatct
     3121 ggtcctgctg gatatcaagg catggaatcc gaaacggtac cgcgccctga caggagggga
     3181 actgcgccct acactggagt ttgcggaacg cctggcctcc ctccgcaagc ccgtgtggct
     3241 gaggtatgtg ctggtacctg gcgttacgga caatctggaa gaaattgagg aactctcccg
     3301 ccatgcggcg cttctgggca acgtggaacg cgtggacgtg ctccctttcc accagctggg
     3361 gagattcaaa tgggatgaat tgaagctgga ctatactttg aaggacgtcc cggaagcgtc
     3421 cgcggaactg gcggaacagg tccgcggcat tttccggcgg ggcggctttc cggattgcgt
     3481 gcattaaccg gattttcgcg ctttggcgcc accaaccgca gcaaaccgga cgcaggcatt
     3541 cccctcttcc ggatgagagg cctcttccct ccactgcaga atccatgcaa aaaggaagac
     3601 gcatttctgg aattgctccg gaatacggca tttccgcaat tcctttcgtt attccatcat
     3661 tctttacggc aacggcagca ttcccgccat ttttccgcca acgccggggg acggcattcc
     3721 gtcccttccc ag
//
