LOCUS       c00027_gnlPro..         6962 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|ALOPODML_27.
ACCESSION   c00027_gnlPro..
VERSION     c00027_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-07 17:15:35
            Original ID  :: gnl|Prokka|ALOPODML_27
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 6962
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     CDS             1..777
                     /locus_tag="ctg27_1"
                     /transl_table=11
                     /translation="LTTWGGTVGMDVDINDRLTAGAAFTANYGSLTASAADTADGDLDS
                     YYVNLFARYQYRKWSHLLILTGGWNDASLTRTVNYGTGSYQARGNTTGAGFGAMYELAY
                     DIALNEDRSVILQPLFNASIVTTRMDGYRESGSAGNAGLKVEDQELTTAAVAVGARLSG
                     LMGSNIFGREALGEVRVNVSQDMGDRRGQANVGFLADPSYTRPVYGAKVGSTAFQIGAG
                     LSVPVGTQGVIFVDGNADIRSGSSSINGSIGYRYNF"
     protocluster    1..6962
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[5383:6962]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      5384..6962
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..6962
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..6962
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             complement(899..1309)
                     /locus_tag="ctg27_2"
                     /transl_table=11
                     /translation="MTDHYYTSETENVGQFRCETTFIPSNVTFATDLPASFEGRAEFPS
                     PGVLLGSTLASCMMSLVSVVAARKGVDLKGMRVLARAAESSKGIEKIELKAIMPLEGSH
                     PLRAMLEKTALSCPVRQALRPDLETPIEWEWK"
     CDS             complement(1508..2671)
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Abhydrolase_1"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg27_3"
                     /sec_met_domain="Abhydrolase_1 (E-value: 2.5e-30, bitscore:
                     94.8, seeds: 48, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MQLPAVQTKFLDLPNSFSLRNGATLDKVRVAYEQYGTLTPNKDNA
                     ILLFHALSGSQHAYGYNPEVPGIGSLWKPENHEGWWNSIIGPGKPLDTNRFCIICANYL
                     GGCYGTTGPATPCPADGQPYGSRFPHVEAADQARLQALLLDSLGIERVHLMGPSVGGLI
                     ALSFACQFPERVRSFISIGSGYRASIEHRLSLFEQILAIELDPDFQGGDYYRGLAPKKG
                     LAFARIIGHKSFVYQEGLEQRARKEVGGNYGLLTWMTPTRSTQSYMLHQGTKFAERFDA
                     NAYIRIADMWAEFDIRDHTPDGTFQTALEGFRRAGIPALIFSIDTDCCFRPAEQQDFAA
                     QLEAAHIPTEFHTIASTKGHDSFLLEPELYAEPIRRILEARKPKGTA"
     CDS             complement(2758..3060)
                     /locus_tag="ctg27_4"
                     /transl_table=11
                     /translation="MLYCIFLIKASLRRRHLTFGKEMTLQFLTKRYISSPEPNTKPELY
                     TMKVLSSLASMKRRHADCQIVKRKGTLYVICKSNPKFKARQGATAGTRLSKKGVK"
     CDS             3210..5135
                     /locus_tag="ctg27_5"
                     /transl_table=11
                     /translation="MTDSSSPDSSPASRNTFAHHLGMKGFLLMEGLLKLVGMKTLYRLG
                     RAAGAVAWHLLPQRRNIVERNLRIVLDPALRGKELQKLSRENFKRTIANFLCSAKTATL
                     TDEQLKHCVTVGGHEQFAAPVLEGRGQVCAIAHSGNWEALARIRAFYPEVERYGSMYRQ
                     FDNPLMEEYVYQRRTERGTQMFSKEGGIKAPMKMLKEGGALGVLSDQFVWEGVYVPFFG
                     KVTGTTPLPALLRKRAGADMVAIAVRTDAPGHWIADMGNVVDFSRSDGSLAGDTIEVNR
                     GLETLIRESVLDVFWMHHRWKSVDRFAPQDKKTAALLENMELKPYRILVAVPRALDEAL
                     ATVPLIRALKTVRCDMQVNIVCPSAQLGIWKTVPEVTHVLPHDSLKQLREALAADEFYN
                     DGPLDLGVMLDGDMETLKALEPYGPMMFSGLDTHPGARKYKFRVKAPVLRAAPPAHRVQ
                     LYLQLGGLHGLDAATPSLFPVKKAAPAEDAPILIAPFSRLGSASEWSWEQWRELVSLLP
                     GRTVLAALEEDRERAASLAEHLNVELAVGAPEALFPVMDGAAAAVAVDGDLPSLCSFRG
                     LPVVTLFSTRLPDVYRPMGTFNRSLYSHQCCSPCFRKECDRDVPCNRHIAVRDVLEALR
                     EISGKE"
     CDS             complement(5384..6163)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 53.7;
                     E-value: 9e-17)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg27_6"
                     /sec_met_domain="Radical_SAM (E-value: 9.6e-32, bitscore:
                     99.6, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPQVFPQNHDPDGESSVTGLVHSVESCGTVDGPGIRFVLFLSGCS
                     LRCRYCHNPDTSYVRRGRLRSADDVLKEIARYRDFLQAAGGGVTLSGGDPLFQPDFAGA
                     VLKGCRKLGLHTCLDTSGHLGVNAGEEMLADTDLVLLDIKAWNPKRYRALTGGELHPTL
                     EFAERLASLRKPVWLRYVLVPGVTDNLEEIEELSRHAALLGNVERVDVLPFHQLGRFKW
                     DELKLDYTLKDVPEASAELAEQVRGIFRQGGFPDCVH"
     CDS             complement(6180..6962)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate2"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg27_7"
                     /sec_met_domain="Gly_radical (E-value: 1.2e-46, bitscore:
                     146.7, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate2 (E-value: 1.3e-52, bitscore:
                     167.9, seeds: 33, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="LNIIHYMHDKYCYERIEMALHDPEILRTMATGIAGLSVAADSLSA
                     IKYATVKAIRNEEGLIVDFKTEGEFPCYGNNDPRVDDIACSLVSNFMEKLRRLHTYRNS
                     LPTQSILTITSNVVYGKKTGNTPDGRRAGEPFAPGANPMHGRDRNGAVASMLSVAKLSY
                     DDSLDGISYTFSIVPQALGKEERERRVKLVSLLDAYFAATGHHINVNVLERKTLLDAMD
                     HPEKYPQLTIRVSGYAVNFIKLTREQQQEVINRTFHTR"
ORIGIN
        1 ctcaccacct ggggaggcac ggtaggcatg gacgtggata tcaacgaccg cctcactgcg
       61 ggagctgcgt ttacagccaa ttacggaagc ctgacggcca gcgcggcgga tacggcggac
      121 ggagacctgg acagttacta tgtcaatctg ttcgcccgct accagtaccg taaatggtcc
      181 catctgctga ttctgacggg cggctggaat gatgcctcgc tgacgcgcac ggtgaattac
      241 ggtacggggt cttaccaggc ccgcggcaat acgacgggag ccggtttcgg cgccatgtac
      301 gagttggcgt atgacatcgc tctcaatgaa gacaggagcg tgattctcca gcctttgttt
      361 aacgcttcca tcgtgacgac ccgcatggac ggctatcggg aaagcggttc tgccggaaat
      421 gccggactga aggtggaaga ccaggaactg actacggctg ctgtcgctgt gggtgcccgg
      481 ctctccggac ttatggggtc caatatcttc gggcgtgaag cgctgggaga agtacgggtg
      541 aacgtttccc aggatatggg agaccgccgc gggcaggcta atgtgggctt cctggcagat
      601 ccctcctata cgcgtccggt gtatggcgcc aaggtcggaa gcacggcctt ccagattggc
      661 gcggggctga gcgttcccgt gggcacccag ggcgtcattt ttgtggacgg caacgcggat
      721 atccgcagcg gctccagctc catcaacggg agtatcggct accgctacaa cttctaaagg
      781 aaaaaacgtc ggccaattgg aaagccgctg cggatgattc cgcagcggct tttttcatga
      841 atgggcggga tttctttaag gataacttca agcttgggtt gacgtccctg atggctgctt
      901 acttccattc ccattcaatg ggcgtttcca gatcaggccg cagcgcctgg cgcacagggc
      961 aggacaaggc ggttttttcc agcatggcgc ggagagggtg gctgccttcc aggggcatga
     1021 ttgccttcag ttcaattttt tcaattccct tggatgattc cgccgcacgc gccagaacgc
     1081 gcatgccttt caaatccact ccttttctgg ccgcgaccac ggaaaccagg ctcatcatgc
     1141 aggaggcgag cgtggaaccg agcaaaactc cgggggaggg gaattctgcg cggccttcaa
     1201 aagaagcggg caggtccgtg gcaaacgtaa cgttggaagg aataaaggtg gtttcgcagc
     1261 ggaattgtcc gacgttctcc gtttcggagg tgtaataatg atcagtcatg aaggtgtgac
     1321 agcgaaaacc ctgttcgggt tcatccggtt caggttattt tcacgttgaa gagccgtgaa
     1381 gttccgcttt gcagggaacc tggccgttcc ttcatgacat ggttgatttt tccctcttgc
     1441 ttccggttcc cggcatcact tctttttaag cgttgctccg ccgtgtcttc ttgcggcggg
     1501 aaaaagctta cgccgtcccc ttcggcttcc ttgcctccag aatgcgccgg atgggttccg
     1561 cataaagttc cggctccagc aggaaggaat cgtgtccctt ggtggaagcg atggtatgga
     1621 actccgtggg aatatgggcg gcttcaagct gcgccgcgaa atcctgctgt tccgccgggc
     1681 ggaaacagca gtctgtatca atggaaaaga taagcgcggg aatccctgcg cggcggaagc
     1741 cttccagggc agtttgaaat gttccgtccg gggtgtggtc gcggatgtcg aactccgccc
     1801 acatatccgc aatacggata taggcgttgg cgtcaaagcg ctccgcgaac ttggttccct
     1861 ggtgaagcat gtagctttgc gtgctgcggg tgggggtcat ccacgtgagc aggccgtagt
     1921 tgcctcccac ctcttttctg gcgcgctgtt ccagcccctc ctggtaaaca aatgatttgt
     1981 ggccgatgat acgggcgaac gccagccctt ttttgggcgc cagtccccgg tagtaatccc
     2041 cgccttggaa atccgggtca agctcaatgg ccaggatttg ttcaaacagg gacaggcggt
     2101 gttcgatgga agcccggtaa ccggagccga tggagatgaa gctccggacc cgttccggga
     2161 actggcacgc gaagctgagg gcgatcaggc cgcccacgga ggggcccata agatgaacgc
     2221 gttctatgcc caggctgtcc agcagaaggg cctggagacg tgcctggtct gcggcctcca
     2281 catgcgggaa gcgggagccg tagggctggc cgtccgcagg gcagggggta gcggggcctg
     2341 tggtgccgta gcagcctccc aggtaattgg cgcagatgat gcagaagcgg tttgtgtcca
     2401 gcggcttgcc ggggccgatg atgctgttcc accagccctc gtggttttcc ggtttccaga
     2461 gggaaccgat gcccgggact tccggattgt aaccgtaggc gtgctggctg ccggaaagag
     2521 catggaacag caggatggcg ttgtctttat tcggcgtcag ggttccgtat tgttcgtagg
     2581 cgacgcgaac cttgtccagc gtagctccat ttctcaggga gaaggagttg ggaagatcca
     2641 ggaatttggt ttggacggca ggaagctgca tggatgtacg gaaaggggct tggtcagcaa
     2701 aaaaagaccg gcacccggcg ggcggcggtc ttcttgaaat ctgtgctggg ccagggatta
     2761 cttaacgccc tttttggaaa ggcgcgtgcc tgccgtagcg ccctggcggg ccttgaactt
     2821 gggattgctc ttgcagatca catacagcgt gcccttgcgc ttcacgattt ggcagtctgc
     2881 gtggcggcgc ttcatagagg ctaaggaaga aagaactttc atggtgtata actccggttt
     2941 ggtgttcggt tcgggagacg aaatataccg tttagttaag aattgtaaag tcatttcttt
     3001 gccaaaagta agatgccgtc tccgcagaga tgcttttatg aggaagatgc agtaaagcat
     3061 gggatggctt tggattccgc gccggcggaa aaatttaggg aagacgtcat ttagccggga
     3121 aaataaaatg cgggaaggga tctctgccgg gggaataaag ggtttgtgga ccggagggga
     3181 ttcgtataac atcttttccg gttttcctta tgactgattc ttcctctcct gattcctccc
     3241 cggcttcccg gaatacgttt gcccatcatt tgggaatgaa agggtttctg ctcatggaag
     3301 ggctgttgaa gctcgtgggc atgaaaacgc tgtaccggct ggggcgcgcg gcaggggccg
     3361 tggcgtggca tctgcttccc cagcgccgga acattgtgga aaggaatctg cgcatcgttt
     3421 tggatccggc cctgcgcgga aaggaactgc agaaattaag ccgggaaaat ttcaaaagga
     3481 ccattgccaa ttttttgtgt tccgcgaaga cggccaccct gacggatgag cagttgaagc
     3541 attgcgtgac ggtcggaggg cacgaacagt ttgccgcgcc tgtgctggaa gggcgcgggc
     3601 aggtttgcgc catagcccac tccggcaact gggaggcgct ggcgagaata cgcgcatttt
     3661 acccggaggt ggagcgttac gggtccatgt accgccagtt tgacaatccg ctgatggagg
     3721 aatacgtgta ccagcggcgc acggaacgtg gaactcagat gttctccaag gagggaggca
     3781 tcaaggctcc catgaaaatg ctcaaggaag ggggagccct gggcgtgctg agcgaccagt
     3841 tcgtatggga gggggtgtat gtcccctttt tcggcaaggt gaccggcacg actccgctgc
     3901 ccgctctgct gagaaaaagg gctggcgcgg acatggtggc catagcggtg cgcacggatg
     3961 ctcccggcca ctggatagcg gatatgggga atgtggtgga tttttccagg tcggacggct
     4021 ctctggcggg ggacacgatt gaagtgaaca gggggctgga aacattgatc cgggaatccg
     4081 tgctggatgt gttctggatg caccatcgct ggaaatccgt tgaccgattt gccccgcagg
     4141 ataaaaaaac ggctgcgctc ctggaaaata tggagctgaa gccgtaccgc attctggtag
     4201 ccgttcccag ggcgctggat gaagccctgg cgactgttcc cctgattcga gctttgaaaa
     4261 ccgtccggtg cgacatgcag gtgaacatcg tctgcccgtc tgcccagctg gggatatgga
     4321 agacggtgcc ggaagtcacc catgtgctgc cgcacgactc cctgaagcag cttcgggaag
     4381 ctttggcagc agatgaattt tacaatgacg gaccgctgga tctgggcgtc atgctggatg
     4441 gagatatgga aaccctgaaa gcgctggaac cgtatggccc catgatgttt tccgggctgg
     4501 acacgcatcc cggtgcccgg aagtacaagt tccgggtaaa ggcccccgtc ctgcgcgccg
     4561 cccccccggc gcaccgggtg cagctttatc tgcaattggg ggggctgcat gggctggacg
     4621 ccgccacccc gtctttgttc cctgtgaaaa aagcggcccc ggcggaagat gcccccattt
     4681 taatcgctcc gttttcccgc ctggggagcg ccagtgaatg gagctgggag cagtggaggg
     4741 agctggtttc ccttctgccg ggacggacgg tgctggcggc tctggaagaa gaccgggagc
     4801 gcgccgcgtc cctggcggaa catttgaatg tggagctggc ggtgggtgcg ccggaagctc
     4861 tctttcccgt catggacggc gctgcggcgg cagtagccgt ggatggcgac ttgccgtccc
     4921 tctgttcttt ccgggggctt ccggtggtga ctctgttttc cacccggctt ccggatgttt
     4981 accgccctat gggcacattc aaccgttccc tttacagcca ccagtgctgt tctccctgct
     5041 tccggaagga atgtgaccgg gatgttccct gcaaccgcca cattgccgtg cgggacgtac
     5101 tggaagcctt gcgggaaatt tccgggaaag aataatctgg gaagggacgg aatgccgtcc
     5161 cccggcgttg gcggaaaaaa cggcgggaat gctgccgttg ccgtaaagaa tgatgaaata
     5221 acgaaaggaa ttgcggaaat gccgtattcc ggagcaattc cagaaatgcg tcttcctttt
     5281 tgcatggatt ctgcagtgga ggggagaggg cccctcatcc ggaagagggg gatgcctgcg
     5341 tccggtttgc tgcggttggt ggcgccaaag cgcgaaaatc cggttaatgc acgcaatccg
     5401 gaaagccgcc ctgccggaaa atgccgcgga cctgttccgc cagttctgcg gacgcttccg
     5461 ggacgtcctt caaggtatag tccagcttca attcatccca tttgaatctc cccagctggt
     5521 ggaaagggag cacgtccacg cgttccacgt tgcccagaag cgccgcatgg cgggagagtt
     5581 cctcaatttc ttccagattg tccgtaacgc caggtaccag cacatacctc agccacacgg
     5641 gcttgcggag ggaggccagg cgttccgcaa actccagtgt agggtgcagt tccccgcctg
     5701 tcagggcgcg ataccgtttc ggattccatg ccttgatatc cagcaggacc agatccgtat
     5761 ccgccagcat ttcttcgccc gcattcacgc ccagatggcc ggaggtgtcc aggcaggtgt
     5821 gcaggcccag tttccggcag cctttcagca cggcaccggc aaaatccggc tggaagaggg
     5881 ggtccccgcc ggagagggtg acgccgccgc ccgccgcctg caaaaaatcc cggtaacggg
     5941 ctatttcctt gaggacatca tctgcgctcc tgagccggcc cctccgcacg taggaggtat
     6001 ccggattatg gcagtagcgg cagcgcaggc tgcaaccgga caggaaaagg acgaagcgga
     6061 tgccggggcc gtccacggtg ccgcaggatt ccacggaatg aaccaaaccg gtgacggagc
     6121 tttctccgtc cggatcatgg ttttgtggaa aaacctgcgg catccgcttg cagtccgggt
     6181 taacgggtgt ggaaagtgcg gttgatgacc tcctgctgct gttcccgggt cagcttgatg
     6241 aaattcacgg catagccgga aacgcggatg gtaagctgcg ggtatttttc cgggtgatcc
     6301 atggcgtcga gaagcgtttt ccgttccagt acgttcacat taatgtggtg acctgtagcg
     6361 gcaaagtagg cgtccagcag ggagacgagc ttgacgcggc gttcacgttc ttccttgccc
     6421 agggcctgag gaacgatgga gaaggtgtag gagatgccgt ccagggagtc gtcataggac
     6481 agcttggcta cggagagcat ggaggccacg gctccgttcc tgtcccttcc gtgcatgggg
     6541 ttggctccgg gggcgaacgg ttccccggct cggcggccgt ccggcgtgtt gcccgtcttt
     6601 ttgccgtaca ccacgttgga ggtgatcgtc aggatggact gggtgggcag ggaattgcgg
     6661 taagtgtgga gcctgcgcag tttttccatg aaattgctga ccaggctgca tgctatgtcg
     6721 tccacgcgcg ggtcattgtt cccgtaacag gggaattccc cttccgtctt gaagtccaca
     6781 atcagccctt cttcattgcg gatggctttt acggtggcat acttgatggc ggacagggag
     6841 tccgccgcca cggaaagccc ggcgattccc gtagccatcg tgcgcagaat ttccggatca
     6901 tgcagcgcca tttcaatgcg ttcatagcag tatttgtcgt gcatgtagtg gatgatgttc
     6961 ag
//
