LOCUS       c00154_gnlPro..         5561 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|AFEGNNAN_154.
ACCESSION   c00154_gnlPro..
VERSION     c00154_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-05 14:43:38
            Original ID  :: gnl|Prokka|AFEGNNAN_154
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 5561
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..5561
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[126:4885]"
                     /cutoff="10000"
                     /detection_rule="(minimum(4, [AcetylCoA_hyd_C,
                     AcetylCoA_hydro, B12-binding, Biotin_lipoyl, Glyoxalase_4,
                     HMGL-like, MM_CoA_mutase, MeaB, OAD_beta, OAD_gamma,
                     PYC_OADA]) and minimum(1, [CT_Btheta, CT_Propionyl_CoA1,
                     CT_Propionyl_CoA2, CT_Propionyl_CoA3, CT_Vparvula]) and not
                     Cytidylate_kin)"
                     /neighbourhood="10000"
                     /product="succinate2propionate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      127..4885
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="10000"
                     /detection_rule="(minimum(4, [AcetylCoA_hyd_C,
                     AcetylCoA_hydro, B12-binding, Biotin_lipoyl, Glyoxalase_4,
                     HMGL-like, MM_CoA_mutase, MeaB, OAD_beta, OAD_gamma,
                     PYC_OADA]) and minimum(1, [CT_Btheta, CT_Propionyl_CoA1,
                     CT_Propionyl_CoA2, CT_Propionyl_CoA3, CT_Vparvula]) and not
                     Cytidylate_kin)"
                     /neighbourhood="10000"
                     /product="succinate2propionate"
                     /protocluster_number="1"
     cand_cluster    1..5561
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(minimum(4, [AcetylCoA_hyd_C,
                     AcetylCoA_hydro, B12-binding, Biotin_lipoyl, Glyoxalase_4,
                     HMGL-like, MM_CoA_mutase, MeaB, OAD_beta, OAD_gamma,
                     PYC_OADA]) and minimum(1, [CT_Btheta, CT_Propionyl_CoA1,
                     CT_Propionyl_CoA2, CT_Propionyl_CoA3, CT_Vparvula]) and not
                     Cytidylate_kin)"
                     /kind="single"
                     /product="succinate2propionate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..5561
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="succinate2propionate"
                     /region_number="1"
                     /rules="(minimum(4, [AcetylCoA_hyd_C, AcetylCoA_hydro,
                     B12-binding, Biotin_lipoyl, Glyoxalase_4, HMGL-like,
                     MM_CoA_mutase, MeaB, OAD_beta, OAD_gamma, PYC_OADA]) and
                     minimum(1, [CT_Btheta, CT_Propionyl_CoA1,
                     CT_Propionyl_CoA2, CT_Propionyl_CoA3, CT_Vparvula]) and not
                     Cytidylate_kin)"
                     /tool="gutsmash"
     CDS             complement(3..89)
                     /locus_tag="ctg154_1"
                     /transl_table=11
                     /translation="MNSAASFSTPPEEEGPCGDWNYHEWAEAF"
     CDS             complement(127..1752)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     succinate2propionate: OAD_beta"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg154_2"
                     /sec_met_domain="OAD_beta (E-value: 7.9e-160, bitscore:
                     520.3, seeds: 229, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MGVFSLSWQMVGMWGIAILLLYLGVAKQYEPLLMVPIAFGALIAN
                     IPDNGMLITQLNQQVISSNEQGEVTATSLNNVGYLRVHVAPLQQAPSKVPANLTTPESR
                     AQYQEIMQQPMQVYPGSQLTVSKIKSVRESQEKAKADAARLGDDSLTVDPNLKDFQNVT
                     EDNGNEPVFLLTNGEGTTVVRQQGVNYFDTSGNRIPVDLKTQKLEPLVVSAAGKYVAVG
                     QHTQELLVTSIHGGLYDWIGLGIKAEIFPPIIFLGVGALTDFGPLLAAPRTLLLGAAAQ
                     VGVAATFFMALFMGFTPNESASIGIIGGADGPTSIFLTMKLAPHLLGAVAVAAYTYMSL
                     VPLIQPPIMALLTTKKERLIRMKSLRTVSKSEKLFFAVLVTIVTILLIPDASPLIGMLM
                     LGNFLRECKVTERLVQASQNEIINIVTIFLGTSVGLTMQGDRFLQSETLLIILLGIVAF
                     GVATAGGVIAAKIMNLIWRKNPVNPLIGSAGVSAVPMAARVSHNVGQKYDPSNYLLMHA
                     MGPNVAGVIGTAVIAGYYIATLAK"
     CDS             complement(1870..2271)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     succinate2propionate: Biotin_lipoyl"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1024:Biotin-requiring enzyme (Score: 58.8; E-value:
                     1.2e-18)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg154_3"
                     /sec_met_domain="Biotin_lipoyl (E-value: 1.4e-19, bitscore:
                     58.8, seeds: 40, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKKLRITVDGKVFDVSVELLDQVSSTTAAPAPAPVAAPAPAASAP
                     VAAPAPAPAPAPAPAAAGAGDVPSPLAGKVVSLDVAAGAPVKAGDQILTLEAMKMNTII
                     YAPASGTLTAFCVNPGDTVQEGQALAKIG"
     CDS             complement(2344..2796)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     succinate2propionate: OAD_gamma"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg154_4"
                     /sec_met_domain="OAD_gamma (E-value: 2.9e-10, bitscore:
                     29.7, seeds: 234, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MLLTTLAFAFAQGIANVMNNLNYQVVGIMVVMLCLGGMAVILTVS
                     GSIAAAIQNRAKAKTAAPVAVSAAAPAAAGKPGMTPEMLAILSAAVDSAMTELTPEMVA
                     VISAAVDAGLDGMSHRIVEIKQSPGSGYAAAGRAEIFASHRIRPSR"
     CDS             complement(2818..4383)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     succinate2propionate: CT_Vparvula"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg154_5"
                     /sec_met_domain="CT_Vparvula (E-value: 6.4e-217, bitscore:
                     709.8, seeds: 132, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAIDPKLIDDLNSRRKKVILSGGQEKIDKRHEKGEMTARDRMGYL
                     FEEGTFSEIGMHVRHNCHNFGMGKKEIPGDGVVSGFGLVDGRPVACAASDFLAQGGSLG
                     YMHAMKIADAQKYALKAGIPMVTVNDSGGARLQEGVAALSGYAQVFYNNVLASGVVPQI
                     SMILGPCAGGAAYSPALTDFIIMRNSGNAGMYITGPKVIEQVTYEKCTMDDIGSAAIHA
                     TVSGNVHFVADSDAHAMDILKRLLSFLPSNNTEEPPHKLDTPLDLSADEGMSDLIPGDN
                     RTPLDVQPIISRLVDNGDFLEVHKDFAKNVVVGFGRICGVVVGIIANQPNVKAGCLDID
                     SSDKAARFIRFCNAFNIPLVNLVDVPGFLPGKNQERGGIIRHGAKLIFAYSQATVPKVT
                     LIMRKAYGGAYIAMCCKDLGADAVFAWPGAEIAVMGAEGAVPVLYGRELKAVEDPAEKA
                     KRQGELLEEYREAFYNPYVAAGMGQITEVINPEETRAKIAFALRTLLNKKEVRPAKKHG
                     NIPL"
     CDS             complement(4415..4885)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     succinate2propionate: Glyoxalase_4"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg154_6"
                     /sec_met_domain="Glyoxalase_4 (E-value: 5.1e-26, bitscore:
                     80.1, seeds: 51, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MYCRPPPCIPYNHHTHMIQQIDHIGIAVKSLDATVPYYRDALGLG
                     EPHIEEVPTQKVRVAMFDVAGVHIELLEPTSPESAIAKAIEKKGEGIHHIAFKTNDIAG
                     NISQAKEAGLTVLNDPPVPGAHNTQVTFLHPKCTFGVLTEFCQHPGGCECGK"
     CDS             5003..5560
                     /locus_tag="ctg154_7"
                     /transl_table=11
                     /translation="MFKSGLAVLFLLASWFSAACAYELLPAHVAVVYNGKSELSRRMAR
                     EYARVRGVPEGNLVSLDCPMTSEISRKEYEDTIRVPLLETARKQRWWVPSGIASSPLMN
                     RKIFVLVLMADLPMKIRHETPAPLPGKGVNQMQTDRAAVDSELALLAVGGYERKSWQVN
                     PYFNKREDFVGSGLPSFLVCRLD"
ORIGIN
        1 gaaaaagcct cagcccattc atgataattc cagtcgccgc acgggccttc ctcctccgga
       61 ggggtggaaa atgaggctgc ggaattcatg ggacgggccg aggccgggag taattttcaa
      121 ttctggttat ttcgccagag tggcgatgta gtagcccgcg atgactgcgg tgccgatcac
      181 gcctgcaacg ttcgggccca tggcgtgcat gagcaggtag ttggaggggt cgtacttctg
      241 gcccacattg tgggagacgc gggctgccat cgggacggcg gagacgcctg cggacccgat
      301 cagggggttg accggattct tgcgccagat gaggttcata atcttggcgg caatcactcc
      361 gcccgctgtt gccacgccga aggcgacgat acccagaagg atgatcagca gcgtttccga
      421 ctgcaggaag cggtcgccct gcatcgtgag ccccacggag gttcccagga agatggtgac
      481 gatgttgatg atttcattct gggaggcctg aaccaggcgt tccgtcacct tgcattcgcg
      541 caggaagttg ccaagcatca gcatgccgat cagcggggat gcgtccggaa tcagcaaaat
      601 ggtgacaatg gttaccagca cggcgaaaaa cagtttttcg ctcttgctga ccgttcgcag
      661 ggatttcatg cggatgaggc gttccttctt ggtcgtcagc aacgccatga tgggcggctg
      721 tatcagcgga accagggaca tgtacgtgta tgcggccacg gccacggcgc ccagcaggtg
      781 cggggccagc ttcatcgtca agaagatgga ggtggggccg tcagcgccgc caatgatgcc
      841 gatggacgcg gattcattcg gggtgaaccc catgaacagg gccatgaaga acgtggcggc
      901 gacgcccacc tgggccgctg cgcccagaag cagggtgcgc ggagcggcaa gcagcgggcc
      961 gaagtccgtc agggctccta cgcccagaaa gatgatgggc gggaaaattt cagccttgat
     1021 gcccagcccg atccagtcat acaggccccc gtggatggag gtgaccagca gttcctgggt
     1081 atgctggcct acggccacgt atttgcctgc ggcggaaacc accaggggct ccagcttctg
     1141 ggttttcaga tccaccggaa tccggttgcc gctggtgtca aagtaattga cgccctgctg
     1201 gcggacgacg gtggtccctt ctccgttcgt gagcaggaag accggttcat tgccgttgtc
     1261 ttccgtcacg ttctggaaat ccttcaggtt ggggtccacc gtcaggctgt cgtcgcccag
     1321 gcgggccgcg tcagcttttg ctttttcctg ggattcccgc acggacttga tcttggaaac
     1381 ggtcagctgg ctgccggggt aaacctgcat gggctgctgc atgatttcct gatactgggc
     1441 acgggattca ggcgtggtca ggttggccgg caccttggag ggagcctgct gaagcggagc
     1501 cacgtgaacg cgcaggtacc ccacgttgtt cagagacgtg gcggttactt ccccctgttc
     1561 attggaggag atgacctgct ggttcagctg ggtgatgagc attccgttat ccggaatgtt
     1621 ggcaatgagg gcgccgaaag caatgggaac catcaggagc ggttcgtatt gcttggctac
     1681 acccagatac aggaggagaa tggcaatgcc ccacatccct accatctgcc atgataggga
     1741 gaagacgccc atcccctgta aaaaggtgat taatgaatca aaaagactca tagggaatat
     1801 ttttgtagcg gtttattatc tgagccggag cccgggactg ttggcgtttc cgggctgccg
     1861 ccgccgggat taaccaattt tggccagtgc ctgtccttcc tgaaccgtgt cgcccgggtt
     1921 cacgcagaaa gcggtgagcg tgccggaggc gggagcgtag atgatggtgt tcatcttcat
     1981 ggcttccagc gtcagaatct ggtcaccggc cttcacgggt gcgcctgctg ccacgtccag
     2041 ggagacgacc ttcccggcaa gcgggctggg aacgtctcct gcgccggcgg cggccggagc
     2101 cggcgcaggt gcgggagccg gggccggggc ggccacgggg gcggaagccg caggagcggg
     2161 agcggccaca ggtgcggggg ccggcgccgc agtggtggag gacacctggt ccagaagttc
     2221 tacggatacg tcaaatactt tgccgtctac ggtgatgcga agtttcttca taatagtgtg
     2281 tggtatggga tattaagtta aatgattccg cctgggcggg gtaagggtga ataatgcagt
     2341 agttcagcgg gaaggccgga tgcggtggga ggcgaaaatt tccgccctgc ccgcagccgc
     2401 atagccggaa cccggggatt gcttgatttc cacgatgcgg tggctcatgc cgtccaggcc
     2461 tgcgtccacg gcggcggaga tgacggccac catttccggc gtaagctccg tcatggcgga
     2521 atccacggcg gcggaaagga tggcgagcat ttccggagtc atgcccggtt tgcccgccgc
     2581 agcgggagcg gcggcagaca cggctacggg agcggctgtt ttggctttgg ccctgttctg
     2641 aatggcggcg gcgatggagc cgctgacggt cagaatgacg gccatgcctc ccaggcacag
     2701 cataaccacc atgatgccga caacctgata gttcagattg ttcataacgt tggcgatgcc
     2761 ctgtgcgaag gcaaaagcga gtgttgtaag taacatatga atccgttggg atggatgtta
     2821 gagcggaatg ttgccgtgtt tcttggccgg gcgcacttcc ttcttgttca gcagggtgcg
     2881 cagggcgaag gcgattttgg cgcgggtttc ttccggattg atgacttccg taatctgtcc
     2941 catgccggcc gccacatacg ggttgtaaaa ggcttcccgg tattcttcca gaagttctcc
     3001 ctggcgcttg gctttttccg ccgggtcttc cacagccttc agttcgcggc cgtagagtac
     3061 cgggacggcg ccttccgcac ccataacggc aatttccgca ccaggccagg cgaagacggc
     3121 gtcagcgccc aggtccttgc agcacatggc gatgtaggcg ccgccgtagg ctttccgcat
     3181 gatcagggtg actttgggca ccgtggcctg ggaataggcg aagataagtt tggcgccgtg
     3241 gcggataatg ccgccccgtt cctggttctt gccgggcagg aagccgggca cgtccaccag
     3301 gttcaccaac ggaatattga acgcgttgca gaaacggatg aagcgcgcgg ccttgtcgga
     3361 ggagtcgata tccaggcagc cggctttcac attgggctgg ttggcgatga tgccgaccac
     3421 cacgccgcag atgcggccga atccgacgac gacatttttg gcgaagtcct tgtgtacttc
     3481 caggaagtcc ccgttgtcca ccagacggct gatgatgggc tggacgtcca gcggcgtgcg
     3541 gttgtcgccg ggaatcaggt cgctcatgcc ttcgtcagcg ctcaggtcca gcggagtgtc
     3601 cagtttgtgg ggcggttctt ccgtgttgtt ggaaggaagg aaggaaagaa gcctcttcag
     3661 gatgtccatg gcgtgcgcgt cgctgtccgc tacgaaatgg acgttgccgg acacggtggc
     3721 gtgaatggcg gcggagccga tgtcgtccat cgtgcatttt tcatacgtga cctgttcaat
     3781 cactttggga ccggtgatgt acatgcccgc gttgccagaa ttgcgcatga tgatgaagtc
     3841 cgtcagggcg ggggagtagg ccgcgccgcc cgcgcagggg cccaggatca tggagatttg
     3901 cggaaccacg ccggaggcaa gcacgttgtt gtaaaacacc tgggcgtatc cggaaagggc
     3961 cgccactcct tcctggaggc gcgcgccgcc ggagtcgttc acggtcacca tcgggatgcc
     4021 ggctttcagc gcatacttct gggcatccgc aattttcatg gcgtgcatgt agccgaggga
     4081 accgccctgg gccaggaaat cggaggccgc gcaggctacc gggcggccgt ccaccaggcc
     4141 gaaaccggaa acgacgccgt cgccggggat ttccttctta cccatgccga agttgtggca
     4201 gttgtggcgc acatgcatgc cgatttccga gaaggtgcct tcctcaaaga ggtaccccat
     4261 gcggtcgcgg gctgtcattt cgcccttttc atgtctcttg tcgatctttt cctgaccgcc
     4321 gctgaggatg actttcttgc ggcggctgtt cagatcgtca atcaatttgg gatcaatagc
     4381 cattggtcta gtggtatggt gaatagggag aatgttactt gccgcattcg cagccgccgg
     4441 ggtgctggca gaattcggtc aggacgccga aggtgcattt gggatgcagg aaggtgacct
     4501 gcgtgttgtg agcgccgggg acgggcgggt cgttcagcac ggtaaggcct gcttccttgg
     4561 cctgggagat atttccggcg atgtcattgg tcttgaaagc gatgtggtgg atgccttccc
     4621 cttttttctc aatagccttg gcgatagcgc tttccggaga ggtgggttcg agcagttcga
     4681 tgtggactcc ggcaacgtca aacatggcga cgcggacctt ttgagtggga acttcctcaa
     4741 tgtggggctc tccgagcccg agcgcatcgc ggtaataggg gacggtggcg tcaagactct
     4801 tgacggcgat gccgatatgg tcaatttgct gaatcatatg cgtgtgatgg ttatagggaa
     4861 tgcagggggg aggcctgcaa tacatcaaga gttattaaag cgtatttttt taggatgtga
     4921 agcgaaaagc ggaaaaatgg cgaattcgtg gccgcccctt tcacctgctt gattgccccg
     4981 gaagatgagg tatggtaggg gcatgttcaa gagtggatta gccgtgttat ttctgctggc
     5041 gtcgtggttt tccgccgcgt gtgcctatga actgctgccc gctcatgtgg ccgtggttta
     5101 taacggaaaa tccgagctga gccgccgcat ggccagggag tatgcacggg tgcgcggcgt
     5161 tccggaaggg aatctcgttt ccctggattg ccccatgacc agcgagattt cgcggaagga
     5221 gtatgaggat acgatccgcg tcccgctgct ggagacggcg cggaaacaac ggtggtgggt
     5281 gccttccggc attgcgtcct cccccttgat gaaccggaag atttttgtgc tggtgctgat
     5341 ggcggacctg ccgatgaaga tacggcatga aacgcccgct cccctgcccg ggaaaggcgt
     5401 caaccagatg cagacggatc gcgctgcggt ggattccgaa ctggctttgc tggcggtggg
     5461 cggttatgaa aggaagtcct ggcaggtgaa tccctatttt aataaaaggg aggattttgt
     5521 gggttccggc cttccttcgt ttctggtatg ccgcctggac c
//
