LOCUS       c00270_gnlPro..         3057 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|AFEGNNAN_270.
ACCESSION   c00270_gnlPro..
VERSION     c00270_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-05 14:43:38
            Original ID  :: gnl|Prokka|AFEGNNAN_270
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 3057
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..3057
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[795:3055]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      796..3055
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..3057
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..3057
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             2..547
                     /locus_tag="ctg270_1"
                     /transl_table=11
                     /translation="PRSTLFPDATLFRFSLFPVKKADPAEDAPILIAPFSRLGSASEWS
                     WEQWRELVSLLPGRTVLAALEEDRERAASLAEHLNVELAVGAPEALFPVMDGAAAAVAV
                     DGDLPSLCSFRGLPVVTLFSTRLPDVYRPMGTFNRSLYSHQCCSPCFRKECDRDVPCNR
                     HIAVRDVLEALREISGKE"
     CDS             complement(796..1575)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 53.7;
                     E-value: 9e-17)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg270_2"
                     /sec_met_domain="Radical_SAM (E-value: 4.1e-32, bitscore:
                     99.6, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPQVFPQNHDPDGESSVTGLVHSVESCGTVDGPGIRFVLFLSGCS
                     LRCRYCHNPDTSYVRRGRLRSADDVLKEIARYRDFLQAAGGGVTLSGGDPLFQPDFAGA
                     VLKGCRKLGLHTCLDTSGHLGVNAGEEMLADTDLVLLDIKAWNPKRYRALTGGELHPTL
                     EFAERLASLRKPVWLRYVLVPGVTDNLEEIEELSRHAALLGNVERVDVLPFHQLGRFKW
                     DELKLDYTLKDVPEASAELAEQVRGIFRQGGFPDCVH"
     CDS             complement(1592..3055)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate2"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg270_3"
                     /sec_met_domain="Gly_radical (E-value: 1.7e-46, bitscore:
                     145.0, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate2 (E-value: 8.9e-176, bitscore:
                     573.4, seeds: 33, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="VKEQNGAAMSLGRVSTFFDIYFTRDLEQGLITEEEVQEIIDQFVM
                     KLRIVRFIRTPDYNNLFSGDPTWVTESIGGMGEDGRTLVTRSSFRMLQTLYNLGPAPEP
                     NLTVLWSRNLPEAFKSFCAKVSIETSSVQYENDDLMRPHWGDDYGIACCVSAMRIGKQM
                     QFFGARANLAKCLLYALNGGVDELKGKQVAPPSPRYTEEILNYDEVMALYDKMQDWLAK
                     TYIDALNIIHYMHDKYCYERIEMALHDPEILRTMATGIAGLSVAADSLSAIKYATVKAI
                     RNEEGLIVDFKTEGEFPCYGNNDPRVDDIACSLVSNFMEKLRRLHTYRNSLPTQSILTI
                     TSNVVYGKKTGNTPDGRRAGEPFAPGANPMHGRDRNGAVASMLSVAKLSYDDSLDGISY
                     TFSIVPQALGKEERERRVKLVSLLDAYFAATGHHINVNVLERKTLLDAMDHPEKYPQLT
                     IRVSGYAVNFIKLTREQQQEVINRTFHTR"
ORIGIN
        1 acctagatct acactctttc ccgacgcgac gctcttccga ttttctttgt tccctgtaaa
       61 aaaagcggac ccggcggaag atgcccccat tttaatcgct ccgttttccc gcctggggag
      121 cgccagtgaa tggagctggg agcagtggag ggagctggtt tcccttctgc cgggacggac
      181 ggtgctggcg gctctggaag aagaccggga gcgcgccgcg tccctggcgg aacatttgaa
      241 tgtggagctg gcggtgggtg cgccggaagc tctctttccc gtcatggacg gcgctgcggc
      301 ggcagtagcc gtggatggcg acttgccgtc cctctgttct ttccgggggc ttccggtggt
      361 gactctgttt tccacccggc ttccggatgt ttaccgccct atgggcacat tcaaccgttc
      421 cctttacagc caccagtgct gttctccctg cttccggaag gaatgtgacc gggatgttcc
      481 ctgcaaccgc cacattgccg tgcgggacgt actggaagcc ttgcgggaaa tttccgggaa
      541 agaataatct gggaagggac ggaatgccgt cccccggcgt tggcggaaaa aacggcggga
      601 atgctgccgt tgccgtaaag aatgatgaaa taacgaaagg aattgcggaa atgccgtatt
      661 ccggagcaat tccagaaatg cgtcttcctt tttgcatgga ttctgcagtg gaggggagag
      721 ggcccctcat ccggaagagg gggatgcctg cgtccggttt gctgcggttg gtggcgccaa
      781 agcgcgaaaa tccggttaat gcacgcaatc cggaaagccg ccctgccgga aaatgccgcg
      841 gacctgttcc gccagttctg cggacgcttc cgggacgtcc ttcaaggtat agtccagctt
      901 caattcatcc catttgaatc tccccagctg gtggaaaggg agcacgtcca cgcgttccac
      961 gttgcccaga agcgccgcat ggcgggagag ttcctcaatt tcttccagat tgtccgtaac
     1021 gccaggtacc agcacatacc tcagccacac gggcttgcgg agggaggcca ggcgttccgc
     1081 aaactccagt gtagggtgca gttccccgcc tgtcagggcg cgataccgtt tcggattcca
     1141 tgccttgata tccagcagga ccagatccgt atccgccagc atttcttcgc ccgcattcac
     1201 gcccagatgg ccggaggtgt ccaggcaggt gtgcaggccc agtttccggc agcctttcag
     1261 cacggcaccg gcaaaatccg gctggaagag ggggtccccg ccggagaggg tgacgccgcc
     1321 gcccgccgcc tgcaaaaaat cccggtaacg ggctatttcc ttgaggacat catctgcgct
     1381 cctgagccgg cccctccgca cgtaggaggt atccggatta tggcagtagc ggcagcgcag
     1441 gctgcaaccg gacaggaaaa ggacgaagcg gatgccgggg ccgtccacgg tgccgcagga
     1501 ttccacggaa tgaaccaaac cggtgacgga gctttctccg tccggatcat ggttttgtgg
     1561 aaaaacctgc ggcatccgct tgcagtccgg gttaacgggt gtggaaagtg cggttgatga
     1621 cctcctgctg ctgttcccgg gtcagcttga tgaaattcac ggcatagccg gaaacgcgga
     1681 tggtaagctg cgggtatttt tccgggtgat ccatggcgtc gagaagcgtt ttccgttcca
     1741 gtacgttcac attaatgtgg tgacctgtag cggcaaagta ggcgtccagc agggagacga
     1801 gcttgacgcg gcgttcacgt tcttccttgc ccagggcctg aggaacgatg gagaaggtgt
     1861 aggagatgcc gtccagggag tcgtcatagg acagcttggc tacggagagc atggaggcca
     1921 cggctccgtt cctgtccctt ccgtgcatgg ggttggctcc gggggcgaac ggttccccgg
     1981 ctcggcggcc gtccggcgtg ttgcccgtct ttttgccgta caccacgttg gaggtgatcg
     2041 tcaggatgga ctgggtgggc agggaattgc ggtaagtgtg gagcctgcgc agtttttcca
     2101 tgaaattgct gaccaggctg catgctatgt cgtccacgcg cgggtcattg ttcccgtaac
     2161 aggggaattc cccttccgtc ttgaagtcca caatcagccc ttcttcattg cggatggctt
     2221 ttacggtggc atacttgatg gcggacaggg agtccgccgc cacggaaagc ccggcgattc
     2281 ccgtagccat cgtgcgcaga atttccggat catgcagcgc catttcaatg cgttcatagc
     2341 agtatttgtc gtgcatgtag tggatgatgt tcagcgcgtc aatgtaagtt ttggcgagcc
     2401 agtcctgcat cttgtcgtac agggccatca cctcatcata gttgagaatt tcctccgtgt
     2461 agcgggggga gggcggggcc acctgcttgc ccttgagttc atccacgccg ccgttcaggg
     2521 cgtagagaag gcacttggcg aggttggcgc gcgctccgaa gaactgcatc tgcttgccga
     2581 tgcgcatggc ggacacgcag catgcgatgc cgtagtcgtc gccccaatgc gggcgcatca
     2641 ggtcgtcgtt ctcgtattgg acggaggacg tttcaatgga aaccttggcg cagaagcttt
     2701 tgaaggcttc cggcaggttc cgggaccaca gaacggtcag gttcggttcc ggagccggcc
     2761 ccaggttgta cagggtctgc agcatgcgga aggaactgcg ggtgacgagg gtgcgcccgt
     2821 cctctcccat gccgccgatg gattccgtca cccaggtggg gtctccggag aacaggttat
     2881 tgtaatcagg cgtgcggatg aaacggacga tgcgcagctt catcacgaac tggtcgatga
     2941 tttcctgaac ttcctcttcc gtgatgagcc cctgttccag gtcgcgggtg aaataaatgt
     3001 cgaagaaagt ggaaacgcgg cccagggaca tggccgctcc attctgttcc ttcacgg
//
