LOCUS       c00253_gnlPro..         5069 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|HCNELFEG_253.
ACCESSION   c00253_gnlPro..
VERSION     c00253_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-05 16:21:33
            Original ID  :: gnl|Prokka|HCNELFEG_253
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 5069
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..5069
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[2:3744]"
                     /cutoff="10000"
                     /detection_rule="(TPP_enzyme_C and not (PFL-like or
                     PFL_6_pcresol or PFL_9_tma or Gly_radical or Gly_430_pcre
                     or Gly_8-9_TMA or PFL_acetate1 or PFL_acetate2 or
                     Gly_radical_scatole or PFL_proline or PFL_toluene) and
                     minimum(2, [ALS_ss_C, AlaDh_PNT_C, Arginase, BKACE,
                     BcrAD_BadFG, Beta_elim_lyase, CPSase_L_D2, Cys_Met_Meta_PP,
                     ELFV_dehydrog, GRDB, Glu_syn_central, Glu_synthase,
                     Gly_reductase, HAAA1, HG2B1, HG2B2, HGD-D, HleuD1, HleuD2,
                     HleuS1, HleuS2, Homoserine_dh, ILVD_EDD, IlvC,
                     Lactoyl-CoA1, Lactoyl-CoA2, Lyase_aromatic, MAAL_C,
                     OKR_DC_1_5_lysine, OKR_DC_1_5_ornithine,
                     OKR_DC_1_7_arginine, OKR_DC_1_C, OTCace, PALP,
                     Proline_reductase, Sacchrp_dh_C, TPL, Thr_dehydrat_C, TnaA,
                     Urocanase]) and not (POR or POR_N or PFOR_II))"
                     /neighbourhood="10000"
                     /product="TPP_AA_metabolism"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      3..3744
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="10000"
                     /detection_rule="(TPP_enzyme_C and not (PFL-like or
                     PFL_6_pcresol or PFL_9_tma or Gly_radical or Gly_430_pcre
                     or Gly_8-9_TMA or PFL_acetate1 or PFL_acetate2 or
                     Gly_radical_scatole or PFL_proline or PFL_toluene) and
                     minimum(2, [ALS_ss_C, AlaDh_PNT_C, Arginase, BKACE,
                     BcrAD_BadFG, Beta_elim_lyase, CPSase_L_D2, Cys_Met_Meta_PP,
                     ELFV_dehydrog, GRDB, Glu_syn_central, Glu_synthase,
                     Gly_reductase, HAAA1, HG2B1, HG2B2, HGD-D, HleuD1, HleuD2,
                     HleuS1, HleuS2, Homoserine_dh, ILVD_EDD, IlvC,
                     Lactoyl-CoA1, Lactoyl-CoA2, Lyase_aromatic, MAAL_C,
                     OKR_DC_1_5_lysine, OKR_DC_1_5_ornithine,
                     OKR_DC_1_7_arginine, OKR_DC_1_C, OTCace, PALP,
                     Proline_reductase, Sacchrp_dh_C, TPL, Thr_dehydrat_C, TnaA,
                     Urocanase]) and not (POR or POR_N or PFOR_II))"
                     /neighbourhood="10000"
                     /product="TPP_AA_metabolism"
                     /protocluster_number="1"
     cand_cluster    1..5069
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(TPP_enzyme_C and not (PFL-like or
                     PFL_6_pcresol or PFL_9_tma or Gly_radical or Gly_430_pcre
                     or Gly_8-9_TMA or PFL_acetate1 or PFL_acetate2 or
                     Gly_radical_scatole or PFL_proline or PFL_toluene) and
                     minimum(2, [ALS_ss_C, AlaDh_PNT_C, Arginase, BKACE,
                     BcrAD_BadFG, Beta_elim_lyase, CPSase_L_D2, Cys_Met_Meta_PP,
                     ELFV_dehydrog, GRDB, Glu_syn_central, Glu_synthase,
                     Gly_reductase, HAAA1, HG2B1, HG2B2, HGD-D, HleuD1, HleuD2,
                     HleuS1, HleuS2, Homoserine_dh, ILVD_EDD, IlvC,
                     Lactoyl-CoA1, Lactoyl-CoA2, Lyase_aromatic, MAAL_C,
                     OKR_DC_1_5_lysine, OKR_DC_1_5_ornithine,
                     OKR_DC_1_7_arginine, OKR_DC_1_C, OTCace, PALP,
                     Proline_reductase, Sacchrp_dh_C, TPL, Thr_dehydrat_C, TnaA,
                     Urocanase]) and not (POR or POR_N or PFOR_II))"
                     /kind="single"
                     /product="TPP_AA_metabolism"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..5069
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="TPP_AA_metabolism"
                     /region_number="1"
                     /rules="(TPP_enzyme_C and not (PFL-like or PFL_6_pcresol or
                     PFL_9_tma or Gly_radical or Gly_430_pcre or Gly_8-9_TMA or
                     PFL_acetate1 or PFL_acetate2 or Gly_radical_scatole or
                     PFL_proline or PFL_toluene) and minimum(2, [ALS_ss_C,
                     AlaDh_PNT_C, Arginase, BKACE, BcrAD_BadFG, Beta_elim_lyase,
                     CPSase_L_D2, Cys_Met_Meta_PP, ELFV_dehydrog, GRDB,
                     Glu_syn_central, Glu_synthase, Gly_reductase, HAAA1, HG2B1,
                     HG2B2, HGD-D, HleuD1, HleuD2, HleuS1, HleuS2,
                     Homoserine_dh, ILVD_EDD, IlvC, Lactoyl-CoA1, Lactoyl-CoA2,
                     Lyase_aromatic, MAAL_C, OKR_DC_1_5_lysine,
                     OKR_DC_1_5_ornithine, OKR_DC_1_7_arginine, OKR_DC_1_C,
                     OTCace, PALP, Proline_reductase, Sacchrp_dh_C, TPL,
                     Thr_dehydrat_C, TnaA, Urocanase]) and not (POR or POR_N or
                     PFOR_II))"
                     /tool="gutsmash"
     CDS             3..1349
                     /gene_functions="biosynthetic (rule-based-clusters)
                     TPP_AA_metabolism: TPP_enzyme_C"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg253_1"
                     /sec_met_domain="TPP_enzyme_C (E-value: 2.1e-51, bitscore:
                     162.2, seeds: 323, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="DVIGITQPITKWAYQIRKPEEIAWAVSRAFYIASTGRPGPVVLDL
                     AKDAQVGLVEYEYKKVDYVRSYQPEPDINKDRIKAAADLINEAKKPFCLVGQGVLLGGA
                     EEELKAFLQKNDIPAGSTVLGLSALPTDFPLNKGMLGMHGNVGPNRKTNECDVLIAIGM
                     RFDDRVTGDLKTYAKQAKIIHLDIDNSEIGKNVAVDVKVLGNAKHTIPMITALLEERKR
                     PEWNAEFDRDEKEEYDKVIEKELYPTEGQLKMGEVVRKVSEATGNDAVLVTDVGQNQMM
                     GVRYFKYKQTRSVVTSGGLGTMGFGLPAAMGAKFGAPDRTVCFFTGDGGMQMTIQELGT
                     IMQEKLNVKIIILNNNFLGMVRQWQELFFHERYSNTIMENPDFVAIAKAYGIAGRAVEK
                     REELDDAIAEMLNHDGAYVLVANVETCGMVYPMVPAGGSVTNMIMGDEK"
     CDS             1365..1919
                     /gene_functions="biosynthetic (rule-based-clusters)
                     TPP_AA_metabolism: ALS_ss_C"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg253_2"
                     /sec_met_domain="ALS_ss_C (E-value: 6.1e-18, bitscore:
                     53.4, seeds: 685, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTTKKLYTVIIFSENIVGLLNQITIIFTRRQLNIETLSVSPSAIK
                     GIHKFTITTFADEDMIDKVVKQIDKRVDILKAYYNTDEDLVYQEIALYKLSTELFIKMG
                     TVEGLIRKYNARILEMNETCVVLEKSGHYDETQALFNELSETIGVLQFIRSGRVAITKS
                     KVERLSDMLADMERKLHEKQQ"
     CDS             1926..2651
                     /locus_tag="ctg253_3"
                     /transl_table=11
                     /translation="MEKVGLFHFVAEPYLMDFRGRVTLPMIGNYLIHAASSHAGERGFG
                     FNDMSERHTAWVLSRLAIEMKEYPTAFDKINLYTWIDEVGRLFTSRCFELADENGKTFG
                     FARSIWAAIDVETRRPTLLDVEALGKYIDERPCPIEKPGKIMPAENKAEGIPYSIKYSD
                     LDINGHFNSVKYIEHLLDLFDIDQFKTREIGRLEIAYQSEGKQGMPLTLHKAESAPDKQ
                     DMAICHEGKAICRAAVTWR"
     CDS             2701..3744
                     /gene_functions="biosynthetic (rule-based-clusters)
                     TPP_AA_metabolism: IlvC"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) IlvN"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg253_4"
                     /sec_met_domain="IlvN (E-value: 7.8e-48, bitscore: 150.6,
                     seeds: 43, tool: rule-based-clusters)"
                     /sec_met_domain="IlvC (E-value: 1e-40, bitscore: 127.8,
                     seeds: 295, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAQMNFGGVVENVMTREEFPLEKAREILKDETIAVIGYGVQGPGQ
                     SLNLRDNGFNVIVGQRQGKTYEKAVEDGWVPGETLFGIEEACDKATIIMCLLSDAAVMS
                     VWPTMKPYLTAGKALYFSHGFAITWNDRTGVVPPTDIDVIMVAPKGSGTSLRSMFLEGR
                     GLNSSFAIYQDATGKAMDRTLALGIGIGSGYLFETTFIREATSDLTGERGSLMGAIQGL
                     LLAQYEVLRENGHTPSEAFNETVEELTQSLMPLFAKNGMDWMYANCSTTAQRGALDWMG
                     PFHDAIKPVVEKLYANVKCGNEAQISIDSNSKPDYREKLEAELKALRESEMWQTAVTVR
                     KLRPENN"
     CDS             complement(3900..4739)
                     /locus_tag="ctg253_5"
                     /transl_table=11
                     /translation="MHKGLLVVGRGIGQVMFQGNALSGLLMLIGIACSSWTLALLALAG
                     NIASTLAAYLLGYSREDIREGLYGFNGTLVGIAIGVFMRINIGSISLLLMGSALSSGIA
                     RQFNRQNKLPGYTAPFILSVWILLILCRYLFPSLMLPTVATPPGHVPDLLHAFTANIGQ
                     VMFQGNMLTGLFFLLAILVNSRLNAAYTIWGASLPLGMILLPATSIADFNMGLLGYNGV
                     LCAIAAGNHLSIRSLIRATLAILLSIGLQIGGMRLGITTLTAPFVLSVWITNLIIKE"
ORIGIN
        1 tcgacgtgat tggcatcacg caaccgatca cgaaatgggc ttatcaaatc cgcaagccgg
       61 aggagatcgc atgggctgtt tctcgtgctt tttatatcgc ttctacaggc cgtccgggtc
      121 cagttgtgct ggatttagcg aaggacgcgc aagtagggtt ggtggaatat gaatataaga
      181 aggtcgatta cgtacgtagt tatcaaccgg agccggatat caataaggat cgtatcaagg
      241 ccgccgccga tttaatcaat gaggcgaaga aacccttctg tctggtcgga cagggagtat
      301 tgttaggcgg tgcggaagag gaattgaaag cgtttctcca aaagaatgat atacctgccg
      361 gatctacggt attgggctta tctgcgttgc caaccgattt cccattgaac aagggaatgc
      421 tcggcatgca tggaaacgta gggccgaacc ggaaaacgaa tgagtgtgat gtattgatcg
      481 ctatcggtat gcgtttcgac gaccgtgtga ctggcgattt aaagacatac gctaagcaag
      541 ccaagattat ccatctggat atcgacaact cggaaatcgg caagaatgtt gccgtggacg
      601 taaaggtgct ggggaatgcc aagcacacga taccaatgat cacggcgttg ttagaagaac
      661 gtaaacgtcc ggaatggaat gccgagttcg atcgggacga gaaagaggaa tacgataagg
      721 taatcgagaa ggaattgtat ccgaccgagg gccaactgaa aatgggcgag gtagtccgta
      781 aggtttccga ggcgaccggt aacgacgctg tattggtgac cgacgttggg cagaaccaga
      841 tgatgggcgt ccgctatttc aaatataaac aaacccgtag cgtggtgact tccggtggtt
      901 taggtacgat gggatttggt ctgccggccg ctatgggcgc taagttcgga gctccggacc
      961 gtacggtctg cttctttacc ggtgatgggg gtatgcagat gacgattcaa gaattgggta
     1021 ccatcatgca ggaaaaactg aacgtgaaga ttatcattct taataataac ttcttaggta
     1081 tggtacgtca gtggcaagaa ttgttcttcc acgagcgtta ttcaaacacc atcatggaga
     1141 atccggattt cgtggcgatc gctaaagcct atggtatcgc cggccgtgcc gttgaaaagc
     1201 gggaggagct ggacgacgct atcgctgaga tgctaaacca cgacggcgct tacgtgttgg
     1261 tcgctaacgt ggagacttgc ggaatggtat atccgatggt tcctgccggg ggaagcgtga
     1321 caaacatgat tatgggagac gagaaataag gaggagatga agctatgaca acaaagaaat
     1381 tatataccgt tattatcttt tcagagaata tcgtaggtct tttgaaccag ataacgatta
     1441 tctttacacg ccgacagctg aacatcgaga ctctgtccgt ctctccgtcc gctatcaagg
     1501 gcatccataa gtttacgatc acgacattcg ccgatgagga tatgatcgat aaggtggtga
     1561 aacagatcga taaacgtgtg gatatcttga aggcttatta taatacggat gaggatttgg
     1621 tttatcaaga gatcgctctt tataagctaa gtaccgagct tttcatcaag atgggaaccg
     1681 tggagggatt gatccgtaaa tacaacgctc gtatcttgga gatgaacgag acctgcgtgg
     1741 tattggagaa gagtggccat tatgacgaga cacaagcctt gttcaatgag ttgagcgaga
     1801 cgatcggtgt cctgcaattc atccgctccg gacgtgtggc tatcacgaag agcaaggtgg
     1861 agcgcttgag cgatatgttg gccgatatgg agcgaaagtt acatgaaaag caacaataat
     1921 aagatatgga gaaagtagga ttatttcatt tcgtggcgga gccgtatttg atggatttta
     1981 ggggacgtgt aacgctccct atgattggta actatttgat tcacgccgct tccagccatg
     2041 ccggagagcg tgggttcggt ttcaatgata tgtcggagcg tcatacggct tgggtgttgt
     2101 cccgtctggc tatcgagatg aaggaatatc ctaccgcctt cgataagatt aatctataca
     2161 cttggatcga tgaggtcgga cgcctcttca ccagccgttg ttttgagctg gcggatgaga
     2221 atgggaaaac ctttggattt gcccgttcta tttgggcggc tatcgacgtg gagacccgcc
     2281 gtcctacctt attggatgta gaggcattgg gcaaatatat cgacgagcgt ccgtgcccga
     2341 tcgagaagcc gggcaagatc atgccggcgg agaataaggc ggagggaatc ccgtattcga
     2401 tcaaatacag cgacttggat atcaacggtc acttcaatag cgtgaagtat atcgagcatc
     2461 tgctggattt gttcgatatc gaccagttca agacccgtga gatcggccgt ctggagatcg
     2521 cctaccaatc cgagggtaaa caaggtatgc cgcttacgct tcataaggcg gagtccgctc
     2581 ccgataagca agacatggct atttgccatg agggaaaggc gatctgtcgt gcggcggtta
     2641 cttggagata ataaaagaga tacataaata ggtttttaaa cattaatagt taaaaagaaa
     2701 atggcacaaa tgaattttgg cggtgttgta gagaatgtaa tgacccgcga ggaatttccg
     2761 ttggaaaagg caagagagat attaaaagat gagacaatcg ctgttatcgg ctatggcgta
     2821 cagggaccgg gacagtcatt gaaccttcgt gacaatggct tcaacgtaat cgttggccag
     2881 cgccaaggta agacttacga gaaagcggtt gaggacggat gggttccggg tgagactctg
     2941 ttcggtatcg aggaggcttg cgacaaggcc acgatcatca tgtgcttatt gtccgacgct
     3001 gccgtaatgt ctgtatggcc tacgatgaag ccttatctga cagctggcaa ggccctgtat
     3061 ttctctcatg gtttcgcgat cacttggaac gatcgtacgg gcgttgttcc tcccacggat
     3121 attgacgtga tcatggtcgc tccgaagggg tctggtacgt ctttgcgttc catgttcctt
     3181 gagggtcgtg gcttgaactc ttctttcgct atctatcaag acgctactgg caaggcaatg
     3241 gatcgtacgt tggcgttggg tatcggtatc ggttccggtt atttgttcga gacgactttc
     3301 atccgtgagg ctacttccga cttgaccggt gagcgcggtt ctttgatggg cgctatccaa
     3361 ggtttgttgt tggcccagta tgaggtattg cgcgagaatg gacatactcc ttccgaggct
     3421 ttcaacgaga cggtcgagga gttgactcaa tccttgatgc ccttattcgc caagaatggt
     3481 atggactgga tgtacgctaa ctgctctacg actgcccagc gtggcgcttt ggactggatg
     3541 ggtccgttcc acgacgcaat caagccggtt gtcgagaaat tgtatgcgaa cgtaaaatgc
     3601 ggtaacgagg ctcagatctc tatcgattct aactctaagc cggattatcg tgagaaattg
     3661 gaggctgaat tgaaggcgtt gcgcgagagc gagatgtggc agactgctgt taccgtacgc
     3721 aagcttcgtc cggagaacaa ctaatccaat cttaatcctt atatatcagc aaaaagaggc
     3781 gggtcactta cgggtggctc gccttgcgtg tccttgcggc cttggccgct gtttctttta
     3841 cggggatgtt gcttaatttc gtcatagtcg taatcattat taaatggatg atgctggaat
     3901 cattccttta ttataaggtt tgttatccat acggagagta cgaatggggc ggtaagggtc
     3961 gtgatcccca gacgcatgcc gccaatctgt aatccgatag atagtaagat cgccaaggtt
     4021 gcccgtatca ggctacggat agaaagatgg ttgcctgccg cgatagcgca taatacaccg
     4081 ttgtagccga gcaaccccat gttaaaatca gcgatgcttg tcgccggtag taatatcatc
     4141 cccaatggca gcgacgcgcc ccatattgtg taggctgcgt ttaagcgtga gtttactaag
     4201 atggcaagaa gaaagaatag ccctgttagc atattgcctt gaaacatcac ttgtccgatg
     4261 ttagcggtga aagcatgaag taaatcgggg acatgcccgg gaggagtcgc tacggttgga
     4321 agcatcaacg aagggaatag atatcgacat aggattaata aaatccatac cgaaagtatg
     4381 aatggggctg tatatcccgg tagtttattc tggcgattga attgccgggc gatcccgctt
     4441 gataaggcgg atcccataag aagcagagat atcgacccga tatttattct catgaatact
     4501 ccgatcgcta tacctaccag tgttccgttg aaaccatata gtccttcgcg tatatcttcg
     4561 cgagaatatc cgaggagata ggccgccagc gtgcttgcga tattgccggc taacgccaat
     4621 aacgctaaag tccatgaact acaggcaata cctatcagca ttaatagtcc ggaaagagca
     4681 ttgccttgaa acatcacttg accgattccc cggcctacaa cgagtaatcc cttatgcata
     4741 ccattttagt tttgactgcg aaaataacga aaatagcaca atcacgagcg taattcgggc
     4801 aactgtgcca agattccggt tacgatatgt tttgcggcat gatcgtcaac ctgtttcttt
     4861 tgcgtttttt tgcaaataac cgcttatatt tttgtttgtt aggattaaat atctaacttt
     4921 ggctcttgtt ataagagata cgaatgacac aagtttgcag atatacggcg ttaacctttt
     4981 ttaccgataa atatttggtg aactggcata attgtttagc aagcaagcaa gcaagcaagc
     5041 aagcaagcaa gcaagcaagc aagcaagca
//
