LOCUS       c00369_gnlPro..         3681 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|EPMGIAPC_369.
ACCESSION   c00369_gnlPro..
VERSION     c00369_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-07 17:35:41
            Original ID  :: gnl|Prokka|EPMGIAPC_369
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 3681
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     CDS             complement(1..255)
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) IlvN"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg369_1"
                     /sec_met_domain="IlvN (E-value: 6.3e-18, bitscore: 53.3,
                     seeds: 43, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAQLNFGGVIENVMTREEFPLEKAREILKDETIAVIGYGVQGPGQ
                     ACNLRDNGFNVIVGQRPGKTYEKAVADGWKPGETLFSIEE"
     protocluster    1..3681
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[1054:3679]"
                     /cutoff="10000"
                     /detection_rule="(TPP_enzyme_C and not (PFL-like or
                     PFL_6_pcresol or PFL_9_tma or Gly_radical or Gly_430_pcre
                     or Gly_8-9_TMA or PFL_acetate1 or PFL_acetate2 or
                     Gly_radical_scatole or PFL_proline or PFL_toluene) and
                     minimum(2, [ALS_ss_C, AlaDh_PNT_C, Arginase, BKACE,
                     BcrAD_BadFG, Beta_elim_lyase, CPSase_L_D2, Cys_Met_Meta_PP,
                     ELFV_dehydrog, GRDB, Glu_syn_central, Glu_synthase,
                     Gly_reductase, HAAA1, HG2B1, HG2B2, HGD-D, HleuD1, HleuD2,
                     HleuS1, HleuS2, Homoserine_dh, ILVD_EDD, IlvC,
                     Lactoyl-CoA1, Lactoyl-CoA2, Lyase_aromatic, MAAL_C,
                     OKR_DC_1_5_lysine, OKR_DC_1_5_ornithine,
                     OKR_DC_1_7_arginine, OKR_DC_1_C, OTCace, PALP,
                     Proline_reductase, Sacchrp_dh_C, TPL, Thr_dehydrat_C, TnaA,
                     Urocanase]) and not (POR or POR_N or PFOR_II))"
                     /neighbourhood="10000"
                     /product="TPP_AA_metabolism"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      1055..3679
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="10000"
                     /detection_rule="(TPP_enzyme_C and not (PFL-like or
                     PFL_6_pcresol or PFL_9_tma or Gly_radical or Gly_430_pcre
                     or Gly_8-9_TMA or PFL_acetate1 or PFL_acetate2 or
                     Gly_radical_scatole or PFL_proline or PFL_toluene) and
                     minimum(2, [ALS_ss_C, AlaDh_PNT_C, Arginase, BKACE,
                     BcrAD_BadFG, Beta_elim_lyase, CPSase_L_D2, Cys_Met_Meta_PP,
                     ELFV_dehydrog, GRDB, Glu_syn_central, Glu_synthase,
                     Gly_reductase, HAAA1, HG2B1, HG2B2, HGD-D, HleuD1, HleuD2,
                     HleuS1, HleuS2, Homoserine_dh, ILVD_EDD, IlvC,
                     Lactoyl-CoA1, Lactoyl-CoA2, Lyase_aromatic, MAAL_C,
                     OKR_DC_1_5_lysine, OKR_DC_1_5_ornithine,
                     OKR_DC_1_7_arginine, OKR_DC_1_C, OTCace, PALP,
                     Proline_reductase, Sacchrp_dh_C, TPL, Thr_dehydrat_C, TnaA,
                     Urocanase]) and not (POR or POR_N or PFOR_II))"
                     /neighbourhood="10000"
                     /product="TPP_AA_metabolism"
                     /protocluster_number="1"
     cand_cluster    1..3681
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(TPP_enzyme_C and not (PFL-like or
                     PFL_6_pcresol or PFL_9_tma or Gly_radical or Gly_430_pcre
                     or Gly_8-9_TMA or PFL_acetate1 or PFL_acetate2 or
                     Gly_radical_scatole or PFL_proline or PFL_toluene) and
                     minimum(2, [ALS_ss_C, AlaDh_PNT_C, Arginase, BKACE,
                     BcrAD_BadFG, Beta_elim_lyase, CPSase_L_D2, Cys_Met_Meta_PP,
                     ELFV_dehydrog, GRDB, Glu_syn_central, Glu_synthase,
                     Gly_reductase, HAAA1, HG2B1, HG2B2, HGD-D, HleuD1, HleuD2,
                     HleuS1, HleuS2, Homoserine_dh, ILVD_EDD, IlvC,
                     Lactoyl-CoA1, Lactoyl-CoA2, Lyase_aromatic, MAAL_C,
                     OKR_DC_1_5_lysine, OKR_DC_1_5_ornithine,
                     OKR_DC_1_7_arginine, OKR_DC_1_C, OTCace, PALP,
                     Proline_reductase, Sacchrp_dh_C, TPL, Thr_dehydrat_C, TnaA,
                     Urocanase]) and not (POR or POR_N or PFOR_II))"
                     /kind="single"
                     /product="TPP_AA_metabolism"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..3681
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="TPP_AA_metabolism"
                     /region_number="1"
                     /rules="(TPP_enzyme_C and not (PFL-like or PFL_6_pcresol or
                     PFL_9_tma or Gly_radical or Gly_430_pcre or Gly_8-9_TMA or
                     PFL_acetate1 or PFL_acetate2 or Gly_radical_scatole or
                     PFL_proline or PFL_toluene) and minimum(2, [ALS_ss_C,
                     AlaDh_PNT_C, Arginase, BKACE, BcrAD_BadFG, Beta_elim_lyase,
                     CPSase_L_D2, Cys_Met_Meta_PP, ELFV_dehydrog, GRDB,
                     Glu_syn_central, Glu_synthase, Gly_reductase, HAAA1, HG2B1,
                     HG2B2, HGD-D, HleuD1, HleuD2, HleuS1, HleuS2,
                     Homoserine_dh, ILVD_EDD, IlvC, Lactoyl-CoA1, Lactoyl-CoA2,
                     Lyase_aromatic, MAAL_C, OKR_DC_1_5_lysine,
                     OKR_DC_1_5_ornithine, OKR_DC_1_7_arginine, OKR_DC_1_C,
                     OTCace, PALP, Proline_reductase, Sacchrp_dh_C, TPL,
                     Thr_dehydrat_C, TnaA, Urocanase]) and not (POR or POR_N or
                     PFOR_II))"
                     /tool="gutsmash"
     CDS             complement(306..1058)
                     /locus_tag="ctg369_2"
                     /transl_table=11
                     /translation="MMSENNKIGTYKFVAEPFHVDFTGRLTMGVLGNHLLNCAGFHASD
                     RGFGIATLNEDNYTWVLSRLAVELDEMPYQYEDFSIQTWVENVYRLFTDRNFAIIDKEG
                     KKIGYARSVWAMISMNTRKPADLLALHSGSIVDYVCDEPCPIEKPSRIKVASKEPVAAL
                     VAKYSDIDINGHVNSIRYIEHILDLFPIEMYKEKRIRRFEMAYIAESYYGDELTLFMDD
                     AGEGVYDVEVKKNGSEVVCRSKVIFTEK"
     CDS             complement(1055..1615)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     TPP_AA_metabolism: ALS_ss_C"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg369_3"
                     /sec_met_domain="ALS_ss_C (E-value: 3e-20, bitscore: 60.8,
                     seeds: 685, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDKKLYTIIVHSENFAGLLNQVTAVFTRRQINIESLNVSASSIKG
                     VHKYTITAWTDKDTIEKVTKQITKKIDVLQAHYFTDDEIYQHEIALYKITTPTLEEKPE
                     VSKVIRKYNARIVEVNAVFAIVEKNGMSEEITNLYEELRQLDCVLQFVRSGRVAITTSC
                     FERVNEYLADREAKYRQSKHQES"
     CDS             complement(1648..3399)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     TPP_AA_metabolism: TPP_enzyme_C"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg369_4"
                     /sec_met_domain="TPP_enzyme_C (E-value: 4.2e-48, bitscore:
                     151.4, seeds: 323, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MADNNKKSNFSSLSQGEGRGEALITGAEALMRSLEHQGVKTIFGY
                     PGGSIMPVFDALYDHRKELNHILVRHEQGATHAVQGFARVSGEVGVCLVTSGPGATNTI
                     TGIADAMIDSTPIVVIAGQVGTNFLGTDAFQEVDLVGITQPITKWSYQIRRAEDVAWAV
                     ARAFYVAKSGRPGPVVLDFAKNAQVEKTEYAPTKVDYVRSYLPVPEMEPEAIRKAAELI
                     NGAERPLVLVGQGVELGEAQQELRAFIEKAGMPAGCTLLGLSALPTNHPLNKGMLGMHG
                     NLGPNINTNKCDVLIAVGMRFDDRVTGKLETYAKQAKIIHFDIDPAEIDKNVKTDVAVL
                     GDCKETLAAVTQLLEPANHQEWIDSFRQYEEVEEEKVIRPELHPAGKALSMGEVVRAVS
                     EATHNEAILVTDVGQNQMMSARYFKYSKERSIVTSGGLGTMGFGLPAAIGATFGRPDRT
                     VCVFMGDGGLQMNLQELGTIMEQKAPVKMILLNNNFLGNVRQWQAMFFNRRYSFTPMMN
                     PDYMKIASAYDIPARRVMTREELAKAIDEMIATDGPFLLEACVEEEGNVMPMTPPGGSV
                     NQMLLEC"
     CDS             complement(3491..3679)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     TPP_AA_metabolism: ILVD_EDD"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg369_5"
                     /sec_met_domain="ILVD_EDD (E-value: 1.2e-16, bitscore:
                     48.8, seeds: 918, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="IKDGDIIEIDIPNRSINVKLTDEELAARLMTPVTRNREVSKALKA
                     YASMVSSADKGAVRLID"
ORIGIN
        1 ctcctctatg gaaaagaggg tttcaccggg cttccatccg tcagccacag ccttttcgta
       61 tgtcttaccc ggacgttgtc ctacaatcac attgaaaccg ttatcacgca agttgcaagc
      121 ctggcccggg ccttgtacgc cataaccgat tactgcaatt gtttcatctt tcaaaatttc
      181 acgtgctttt tccaaaggaa attcttcgcg ggtcattaca ttctcaataa caccgccaaa
      241 attcaattgt gccatttttt gttttaattt ttatttgtgg cttacgccaa cgttgataaa
      301 tttatttatt tctctgtaaa tatcacttta gagcgacaga ccacttcgct gccattcttt
      361 ttcacctcca catcgtaaac gccctcaccg gcatcatcca tgaacaaagt caactcatcc
      421 ccataatagc tttcggctat atatgccatt tcaaaacgac ggatgcgctt ctccttatac
      481 atctctatcg ggaacaaatc aaggatatgc tcgatgtaac ggatgctgtt cacatgcccg
      541 ttaatgtcaa tatcactgta tttggctacc aaagcagcaa ccggttcctt tgaggccacc
      601 tttatccgcg aaggtttttc tatcgggcaa ggctcatcgc aaacataatc tacaatgctg
      661 ccactatgaa gagccagcaa atctgccggc ttacgggtat tcatgctaat catcgcccaa
      721 acggaacgtg catacccaat cttcttaccc tccttatcga tgatagcgaa attacggtct
      781 gtaaaaaggc gatagacatt ctccacccaa gtctgaatgg agaaatcttc gtactgataa
      841 ggcatttcat ccagttcgac agccagacgg gacaataccc aagtataatt gtcctcgttt
      901 agagtcgcga tgccaaaacc gcggtcgctg gcatggaaac ccgcacaatt caacaaatgg
      961 ttgcccaata ctcccattgt cagccgtccg gtaaagtcga catgaaacgg ttcggcaaca
     1021 aacttgtagg tacctatctt attgttctcg ctcatcatga ttcctggtgt ttactttgac
     1081 gatattttgc ttcacggtcc gccagatatt cattgacacg ctcaaagcag ctggtcgtaa
     1141 tggctacgcg acccgaacgg acgaactgaa gtacgcaatc caactggcgc aactcctcat
     1201 aaagattggt tatttcctca ctcatgccat tcttctcaac aatggcaaat acagcattca
     1261 cctccacaat acgggcattg tacttacgga ttactttgga cacctccggt ttttcttcca
     1321 gcgtaggggt cgttatttta taaagggcaa tctcgtgctg atagatttca tcatccgtga
     1381 aataatgggc ttgcagcaca tctattttct tggtaatctg cttggtcacc ttttcaatgg
     1441 tatccttgtc cgtccaagcg gtaatggtat atttatgtac ccccttgatg gaggaggccg
     1501 atacattcaa actctcgatg tttatctgcc ggcgggtaaa cacagccgtc acctgattca
     1561 gcaaaccggc aaagttttcc gaatgaacaa tgattgtata tagtttttta tccatagata
     1621 gagtaagttc tttaatttcg catgacttca gcattccaac aacatctgat tgaccgaacc
     1681 gcccggaggt gtcatcggca ttacatttcc ttcttcctcc acacaagctt cgagcaggaa
     1741 cggtccgtca gtcgctatca tctcgtcaat cgcttttgcc agctcctcgc gggtcattac
     1801 cctgcgtgca ggaatatcat atgccgaagc aatcttcata taatccggat tcatcatcgg
     1861 agtaaacgaa taacgacgat tgaagaacat cgcctgccat tgacgaacat tgcccaggaa
     1921 gttattgttc aacagaatca tcttcaccgg agccttttgt tccatgatgg tacccagttc
     1981 ctgcaggttc atctgcaagc cgccgtctcc catgaatacg cataccgtgc ggtccggacg
     2041 tccaaaagtg gcaccgatag ctgccggaag gccgaagccc atagtaccca ggccaccgga
     2101 agtaacaatg ctacgttcct tgctgtattt gaaataacgg gcggacatca tctgattctg
     2161 gccgacatca gtcaccaata tcgcctcgtt atgggtagcc tcactcacag cacgcaccac
     2221 ctcacccatg cttagcgctt ttcctgccgg atgtaattcc ggacggatta ccttttcctc
     2281 ttccacttcc tcgtattgcc ggaaactgtc aatccattcc tgatggttgg cgggttccag
     2341 caattgggta acggcagcca gtgtctcttt gcaatctccc aggacagcaa catcggtctt
     2401 tacattctta tctatttcgg cagggtcaat atcaaagtgg ataatcttcg cctgcttggc
     2461 atatgtttcc aatttacccg tcacacggtc atcaaagcgc atgcctacgg cgataaggac
     2521 atcacattta ttggtattga tattgggccc caggttaccg tgcataccaa gcattccttt
     2581 gttcaacgga tggttcgtag gtaatgcgga aagtcccaat aaagtgcatc cggcaggcat
     2641 tccggctttc tcaatgaaag cacgaagctc ttgctgggct tctcccagct ccacgccttg
     2701 tcctaccagc accaaaggcc gttccgcacc atttattaat tcagcagcct tccgtatggc
     2761 ctccggctcc atttcgggaa cgggaagata gctacggaca tagtccacct tagtaggcgc
     2821 atattctgtt ttctccactt gggcgttttt agcaaaatcc agcacgaccg gtcccggacg
     2881 tccgctcttt gccacataga aagcacgtgc cactgcccag gccacatctt ctgcacgacg
     2941 aatctggtaa ctccatttcg tgataggctg agtaatgcct accaagtcca cttcctgaaa
     3001 tgcatccgta cccaagaagt tcgtccctac ctggccggca atgacaacga taggcgtact
     3061 gtcaatcatg gcatctgcaa tgcccgtgat agtattggta gcacccggtc cactggtaac
     3121 cagacaaacg ccaacctcgc ctgatacacg tgcaaagcct tgtaccgcat gggttgctcc
     3181 ttgttcatgg cgaaccaaga tgtggttcaa ttccttccgg tggtcgtata aggcatcgaa
     3241 taccggcatg atgctccctc ccggataacc gaaaatagtc tttactccct gatgctccag
     3301 agagcgcatc aatgcttctg cgcctgttat tagagcctca ccccgaccct ctccttggga
     3361 gagggaggaa aagttactct ttttattatt atcagccata ctttatctat tcattatatt
     3421 taaaagctca atagtagctt gaaaaatttg tttcattctc tcctcccctc gctttcggaa
     3481 aggggggctt ttaatcaatc agcctcacag cccctttgtc agcggaactc accatactgg
     3541 catatgcttt caatgctttg gagacttcac ggttgcgggt taccggagtc ataaggcgtg
     3601 ctgcaagctc ttcatccgtc aacttcacat tgatagaacg gttcggtata tcaatttcga
     3661 taatgtcccc atccttaatc t
//
