LOCUS       c00396_gnlPro..         1716 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|PILIIIBM_396.
ACCESSION   c00396_gnlPro..
VERSION     c00396_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-07 18:11:34
            Original ID  :: gnl|Prokka|PILIIIBM_396
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 1716
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     CDS             complement(1..540)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 55.9;
                     E-value: 1.9e-17)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg396_1"
                     /sec_met_domain="Radical_SAM (E-value: 1.6e-26, bitscore:
                     80.8, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPQVFPQNHDPDGESSVTGLVHSVESCGTVDGPGIRFVLFLSGCS
                     LRCRYCHNPDTSYVRRGQLRSADDVLKEIARYRDFLQAAGGGVTLSGGDPLFQPDFAGA
                     VLKGCRKLGLHTCLDTSGHLGVNAGEEMLADTDLVLLDIKAWNPKRYRALTGGELRPTL
                     EFAERLPDQRQRELLLA"
     protocluster    1..1716
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[0:1714]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      1..1714
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..1716
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..1716
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             complement(557..1714)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate2"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg396_2"
                     /sec_met_domain="Gly_radical (E-value: 2.9e-47, bitscore:
                     146.9, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate2 (E-value: 1.9e-116, bitscore:
                     376.9, seeds: 33, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="EPNLTVLWSRNLPEAFKSFCAKVSIETSSVQYENDDLMRPHWGDD
                     YGIACCVSAMRIGKQMQFFGARANLAKCLLYALNGGVDELKGKQVAPPSPRYTEEILNY
                     DEVMALYDKMQDWLAKTYIDALNIIHYMHDKYCYERIEMALHDPEILRTMATGIAGLSV
                     AADSLSAIKYATVKAIRNEEGLIVDFKTEGEFPCYGNNDPRVDDIACSLVSNFMEKLRR
                     LHTYRNSLPTQSILTITSNVVYGKKTGNTPDGRRAGEPFAPGANPMHGRDRNGAVASML
                     SVAKLSYDDSLDGISYTFSIVPQALGKEERERRVKLVSLLDAYFAATGHHINVNVLERE
                     TLLDAMDHPEKYPQLTIRVSGYAVNFIKLTREQQQEVINRTFHTR"
ORIGIN
        1 ggccagcagc agctcccgct ggcgctggtc gggcaggcgt tccgcaaact ccagtgtggg
       61 gcgcagttct ccgcctgtca gggcgcgata ccgtttcgga ttccatgcct tgatatccag
      121 caggaccagg tccgtatccg ccagcatttc ttcgcccgca ttcacgccca gatggccgga
      181 ggtgtccagg caggtgtgca ggcccagttt ccggcagcct ttcagcacgg caccggcaaa
      241 atccggctgg aagaggggat ccccgccgga gagggtgact ccgccgcccg ccgcctgcaa
      301 aaaatcccgg taacgggcta tttccttgag gacatcatct gcgctcctga gctggcccct
      361 ccgcacgtag gaggtatccg gattatggca gtagcggcaa cgcaggctgc agccggacag
      421 gaaaaggacg aagcggatgc cggggccgtc cacggtgccg caggattcca cggaatgaac
      481 caaaccggtg acggagcttt ctccgtccgg atcatggttt tgtggaaaaa cctgcggcat
      541 ccgcttgcag tccgggttaa cgggtgtgga aagtgcggtt gatgacctcc tgctgctgtt
      601 cccgggtcag cttgatgaaa ttcacggcat agccggaaac gcggatggta agctgcgggt
      661 atttttccgg gtgatccatg gcgtcgagaa gcgtttcccg ttccagtacg ttcacattaa
      721 tgtggtgacc tgtagcggca aagtaggcgt ccagcaggga gacgagcttg acgcggcgtt
      781 cacgttcctc cttgcccaag gcctgaggaa cgatggagaa ggtgtaggag atgccgtcca
      841 gggagtcgtc ataggacagc ttggctacgg agagcatgga ggccacggct ccgttcctgt
      901 cccttccgtg catggggttg gctccggggg cgaacggttc cccggctcgg cggccgtccg
      961 gcgtgttgcc cgtctttttg ccgtacacca cgttggaggt gatcgtcagg atggactggg
     1021 tgggcaggga attgcggtaa gtgtggagcc tgcgcagttt ttccatgaaa ttgctgacca
     1081 ggctgcatgc tatgtcgtcc acgcgcgggt cattgttccc gtaacagggg aattcccctt
     1141 ccgtcttgaa gtccacaatc agcccttctt cattgcggat ggcttttacg gtggcatact
     1201 tgatggcgga cagggagtcc gccgccacgg aaagcccggc gattcccgta gccatcgtgc
     1261 gcagaatttc cggatcatgc agcgccattt caatgcgttc atagcagtat ttgtcgtgca
     1321 tgtagtggat gatgttcagc gcgtcaatgt aagttttggc gagccagtcc tgcatcttgt
     1381 cgtacagggc catcacctca tcatagttga gaatttcctc cgtgtagcgg ggggagggcg
     1441 gggccacctg cttgcccttg agttcatcca cgccgccgtt cagggcgtag agaaggcact
     1501 tggcgaggtt ggcgcgcgct ccgaagaact gcatctgctt gccgatgcgc atggcggaca
     1561 cgcagcatgc gatgccgtag tcgtcgcccc aatgcgggcg catcaggtcg tcgttctcgt
     1621 attggacgga ggacgtttca atggaaacct tggcgcagaa gcttttgaag gcttccggca
     1681 ggttccggga ccacagaacg gtcaggttcg gttccg
//
