LOCUS       c00288_gnlPro..         3665 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|EMICEFEF_288.
ACCESSION   c00288_gnlPro..
VERSION     c00288_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-07 22:45:51
            Original ID  :: gnl|Prokka|EMICEFEF_288
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 3665
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..3665
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[47:3512]"
                     /cutoff="10000"
                     /detection_rule="(Succ_DH_flav_C and FAD_binding_2 and
                     (Fer2_3 or Fer4_7 or SucD_FumR))"
                     /neighbourhood="10000"
                     /product="Fumarate2succinate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      48..3512
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="10000"
                     /detection_rule="(Succ_DH_flav_C and FAD_binding_2 and
                     (Fer2_3 or Fer4_7 or SucD_FumR))"
                     /neighbourhood="10000"
                     /product="Fumarate2succinate"
                     /protocluster_number="1"
     cand_cluster    1..3665
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(Succ_DH_flav_C and FAD_binding_2 and
                     (Fer2_3 or Fer4_7 or SucD_FumR))"
                     /kind="single"
                     /product="Fumarate2succinate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..3665
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Fumarate2succinate"
                     /region_number="1"
                     /rules="(Succ_DH_flav_C and FAD_binding_2 and (Fer2_3 or
                     Fer4_7 or SucD_FumR))"
                     /tool="gutsmash"
     CDS             48..740
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Fumarate2succinate: SucD_FumR"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg288_1"
                     /sec_met_domain="SucD_FumR (E-value: 3.1e-75, bitscore:
                     239.8, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MWLLNSSIGKKLIMSISGLFLVLFLLFHLSMNVAAVFSGEAYNMV
                     CSLLGSNWYAVAATLVLAAGVVIHFVYAIILTLQNRKARGNDRYAINARPKGVEWASQN
                     MFVLGLIVILFMLLHFSQFWYKMMFAELIGHHEVALGSAMVSPQDGAAFINYYFQGNAV
                     ITVLYLIWYVALWFHLTHGFWSAIQTIGWNNTIWMNRWECISKIVATVICGLFAIITII
                     FFLNGVGA"
     CDS             789..2732
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Fumarate2succinate: FAD_binding_2"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Fumarate2succinate: Succ_DH_flav_C"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1027:FAD binding domain (Score: 197.2; E-value:
                     1.7e-60)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg288_2"
                     /sec_met_domain="FAD_binding_2 (E-value: 8.3e-62, bitscore:
                     197.2, seeds: 62, tool: rule-based-clusters)"
                     /sec_met_domain="Succ_DH_flav_C (E-value: 2.2e-39,
                     bitscore: 122.2, seeds: 739, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSEINSKIPQGPLAEKWTNYKNHQKLVNPANKRRLDVIVVGTGLA
                     GASAAASLAEMGFNVLNFCIQDSPRRAHSIAAQGGINAAKNYQNDGDSVYRLFYDTIKG
                     GDYRAREANVYRLAEVSNSIIDQCVAQGVPFAREYGGLLDNRSFGGAQVSRTFYARGQT
                     GQQLLLGAYSALSRQIGLGKVKMYTRHEMLDVVKVDGRARGIIARNLITGKIERYAAHA
                     VVVATGGYVNTFFLSTNAMASNGSAAWQCYKKGAYFANPCMVQIHPTCVPVHGDYQSKL
                     TLMSESLRNDGRIWVPKKLEDAKALQAGTKKGKDIPEADRDYYLERRYPAFGNLVPRDV
                     ASRAAKERCDAGFGVNNTGLAVFLDFSDAINRLGKEVVKQKYGNLFDMYEEITNDDPYE
                     TPMMIYPALHYSMGGLWVDYELMTSIPGLFAIGEANFSDHGANRLGASALMQGLADGYF
                     VLPYTIQNYLSDQIQVPRFSTDLPEFNEAEKNIQARINKLMSIKGKESVDSIHKRLGHI
                     MWEHIGMARNAESLKEAIQMLKDLKKEFWSNVFIPGNADDLNVELEKALRLADFIEIGT
                     LMAHDALDRAESCGGHFREEHQTEEGEALRHDDKFMYVSCWEYQGEDKDPVMLKEDLNY
                     EFVVPQTRNYKK"
     CDS             2757..3512
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Fumarate2succinate: Fer2_3"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Fer4_8"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Fer4_4"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg288_3"
                     /sec_met_domain="Fer2_3 (E-value: 1.9e-26, bitscore: 80.0,
                     seeds: 38, tool: rule-based-clusters)"
                     /sec_met_domain="Fer4_8 (E-value: 6.7e-13, bitscore: 36.8,
                     seeds: 153, tool: rule-based-clusters)"
                     /sec_met_domain="Fer4_4 (E-value: 7.1e-07, bitscore: 17.5,
                     seeds: 79, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDKDINITLKVWRQKGPNVKGQFETYQLKNINQGTSFLEMLDILN
                     EQLISEGKEPVVFDHDCREGICGMCSLYVNGHPHGPATGATTCQLYMRRFNDGETITIE
                     PWRSAGFPVIRDLMVDRYAYDKIIQAGGFTSVNTGGVPDANAIPIPKPDAEMAMDAAAC
                     IGCGACAAACKNGSAMLFVSAKVSQLALLPQGKVEAARRAKAMVAKMDELGFGNCTNTR
                     ACEMECPKNISVSNIARLNREFICAKLQD"
ORIGIN
        1 tcgtgggctc ggagatgtgt ataagagaca gcttaaatta ttatcttatg tggttactta
       61 attcttctat cgggaagaag ctgataatga gcatatcagg tctcttcctt gttttgttct
      121 tattgttcca cctgtcgatg aacgtggctg ccgttttctc cggagaggct tataacatgg
      181 tttgttcgct gttgggctct aattggtacg ctgtggcagc tactctcgta ttggccgcag
      241 gcgttgtgat ccacttcgtg tacgctatca ttcttacctt gcaaaaccgt aaggcacgtg
      301 gtaacgaccg ttacgctatc aacgctcgtc cgaaaggcgt agaatgggca tctcagaaca
      361 tgttcgtttt gggtttgatc gttatcctgt tcatgctgtt gcacttcagc cagttctggt
      421 ataagatgat gttcgccgaa ttgatcggtc atcacgaagt agcattgggg tcagctatgg
      481 tatctccaca agacggagcc gcttttatca actattactt ccaaggtaat gccgtgatca
      541 ccgttcttta tttgatctgg tacgtggctt tgtggttcca cttgactcac ggtttctgga
      601 gcgctatcca aacaatcggt tggaataaca cgatctggat gaaccgttgg gaatgtatct
      661 ctaagatcgt agctacggtt atttgcggac ttttcgcaat cattactatc atcttcttcc
      721 taaacggagt aggtgcttaa ttgcaagcta caggttttat aattaagaac ttttgaaatt
      781 aaatcattat gtctgaaata aattctaaaa tacctcaagg cccgttggct gagaagtgga
      841 ctaattataa aaatcaccag aaattggtga accccgctaa caagcgtcgt ttggatgtta
      901 tcgtagtcgg tacaggtttg gctggagctt ccgctgccgc atctttggca gagatgggat
      961 tcaacgtatt gaacttctgt atccaagatt ctccccgccg tgcgcactct atcgccgctc
     1021 aaggtggtat caacgccgct aagaactacc agaatgatgg tgactctgtt taccgtttgt
     1081 tctacgatac gatcaaaggt ggtgactacc gtgctcgtga ggctaacgtt tatcgtttgg
     1141 ctgaggtttc taactcaatc atcgaccagt gcgtggctca aggcgttcct ttcgctcgcg
     1201 aatatggtgg tttgttggat aaccgttcat tcggtggcgc tcaggtatcg cgtacgttct
     1261 acgcccgtgg ccagacgggt cagcagcttt tgttaggcgc ttattcagcg ttgagtcgtc
     1321 agatcggttt gggtaaggtt aagatgtaca ctcgtcatga gatgttggat gtagttaagg
     1381 tagacggtcg tgctcgtggt atcatcgccc gcaacttgat tacaggtaag atcgaacgtt
     1441 acgccgctca cgctgtagtt gtagctacag gaggttacgt taatacgttc ttcttgtcaa
     1501 ccaacgcgat ggcatctaac ggttctgccg catggcaatg ctacaagaaa ggtgcttact
     1561 tcgctaatcc ttgtatggtt cagattcacc cgacttgcgt accggtacac ggtgactatc
     1621 agtctaaatt gactttgatg tccgagtctt tgcgtaatga tggccgtatc tgggttccga
     1681 agaaactgga agacgctaag gctttacagg caggaacaaa gaaaggtaaa gatatacctg
     1741 aggcagatcg cgattattat ttggaacgtc gttatccggc attcggaaac ttggttcctc
     1801 gcgacgtggc ttctcgtgcc gctaaagagc gttgcgacgc tggcttcggt gttaataaca
     1861 caggtttggc tgtattcttg gacttctccg acgctatcaa ccgtctgggt aaagaggttg
     1921 taaaacagaa atatggtaac ttgttcgata tgtacgaaga aatcaccaac gacgatccat
     1981 acgagactcc gatgatgatc tacccggcat tgcactattc aatgggtggt ctgtgggttg
     2041 attatgaatt gatgacttct atcccgggtc tgttcgctat cggtgaggca aacttctctg
     2101 atcacggcgc aaaccgtttg ggtgcttccg cgttgatgca aggtttggcc gatggttact
     2161 tcgtattgcc ttatacgatc cagaactatt tgtctgacca gattcaggtt ccgcgtttca
     2221 gcaccgatct gcctgagttc aacgaggctg agaagaatat ccaagcccgt atcaataaat
     2281 tgatgagcat caagggtaaa gagtctgttg attctatcca caaacgtttg ggacacatca
     2341 tgtgggaaca catcggtatg gctcgtaacg cagagagctt gaaagaggct atccagatgt
     2401 tgaaagactt gaagaaggaa ttctggagca acgtattcat tccgggtaac gcagatgact
     2461 tgaacgtaga gttggagaaa gcgttacgtt tggcagactt tatcgagatc ggtaccttga
     2521 tggcacatga cgctttagac cgtgctgaat cttgtggtgg tcacttccgt gaggagcacc
     2581 agactgagga aggcgaggca ttacgtcacg atgacaaatt catgtatgtc tcttgctggg
     2641 aatatcaggg tgaggataaa gaccctgtta tgctgaagga agatttgaac tatgaattcg
     2701 tagttcctca gacacgtaac tataagaagt aatcaatcat tgcaaaaatt agaatcatgg
     2761 ataaagatat aaatatcaca ctcaaagttt ggcgtcagaa aggtccgaat gtaaaaggac
     2821 aattcgaaac ttaccaactt aaaaacatca accaaggcac ttctttcttg gaaatgttgg
     2881 atatcctgaa cgagcaatta atcagcgaag gtaaagaacc tgttgttttt gaccatgact
     2941 gtcgtgaagg tatctgcggt atgtgttcat tgtatgtaaa cggacatccg cacggaccgg
     3001 caaccggagc tacgacttgc caattatata tgcgccgttt caacgatggt gagactatca
     3061 cgatcgaacc gtggcgttcc gccggtttcc cggtaatccg cgacttgatg gtagaccgtt
     3121 acgcttacga taaaatcatt caagctggtg gtttcacatc cgtaaatacc ggtggtgtac
     3181 ccgacgcaaa cgcaattcct attcctaagc cagacgcaga gatggcaatg gatgccgctg
     3241 cttgtatcgg ttgtggcgct tgtgccgccg cttgtaagaa cggttcagct atgttgttcg
     3301 tatccgctaa ggttagccag ttggctttat tacctcaagg taaggtagag gctgcccgcc
     3361 gtgcgaaagc aatggtcgct aaaatggatg aacttggctt cggtaactgt acgaacacac
     3421 gcgcttgcga gatggagtgt ccgaagaaca tctccgttag taacatcgct cgtttgaacc
     3481 gtgagttcat ttgcgctaag ctacaagact aatatttgat tagtatatca tggaaaaggc
     3541 gtctttctct aaaagaaagg cgccttttta tttttgttat atgttgaaaa acataattta
     3601 gcactacaga tcttgacagg aattgaaaag tccgtatatt tgcattgtgg ttttttcata
     3661 atagt
//
