LOCUS       c00384_gnlPro..         3136 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|EMICEFEF_384.
ACCESSION   c00384_gnlPro..
VERSION     c00384_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-07 22:45:51
            Original ID  :: gnl|Prokka|EMICEFEF_384
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 3136
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..3136
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[172:3136]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      173..3136
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..3136
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..3136
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             173..2413
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate2"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg384_1"
                     /sec_met_domain="Gly_radical (E-value: 7.8e-47, bitscore:
                     145.5, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate2 (E-value: 6.1e-283, bitscore:
                     926.7, seeds: 33, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKFENECWDHFKGETWKREINVRDFIQSNYTPYEGDDSFLVASSE
                     KTRKVWNKLTEMFKVEREKGVYDAETKLPQGIDVYGPGYIDKENEVIVGLQTDAPLKRG
                     IFPKGGIRMVENSLEAYGYHLDPMTKEIFTKYRKTHNEGVFSAYTEEMIAARRSAIITG
                     LPDAYGRGRIIGDYRRVALYGTAILIEEKKRFLNRLDIQEITEEIIQSREEISEQIKAL
                     KAFEKMCASYGFDVTRPAQNAREAVQFVYLAYLAAVKDQDGAAMSIGRTSTFLDIYIEK
                     DIREGKLTEEEAQELVDQLIIKLRIVRFLRTPEYNDLFSGDPVWVTESLGGQGVDGRSL
                     VTRTSYRYLHTLYNLGPAPEPNLTVLWFKNAPENWKRFCAKVSIDTSAIQYENDDLMRP
                     DYGDDYGIACCVSPMKIGKQMQFFGARANLAKCLLYAINGGRDERSGVQVAPMFEPVRG
                     EYLEYDEVMAKYEQMMRWLAKVYVNALKIIHYMHDKYAYEAFEMSLHDGDVERIRATGI
                     AGLSIVADSLAAIRDTKVRVIRDERGLAVDFEREGEYVPFGNNDDRTDSIAVEITEKFM
                     EYLRQHETYRHAKPTQSILTITSNVVYGKKTGTTPDGRIGGTPFAPGANPMNGRDTKGA
                     IAALASVAKLPFQHAHDGISYTFAVSPATLGKEREVQINNLVSLLDGYFTPDGGQHLNV
                     NVFDKDLLIDAMEHPEKYPQLTIRVSGYAVNFVKLTREQQLDVISRTINHSL"
     CDS             2414..3136
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 67.6;
                     E-value: 4.5e-21)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg384_2"
                     /sec_met_domain="Radical_SAM (E-value: 2.5e-34, bitscore:
                     106.2, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="VIKGKIHSLESFGTVDGPGIRFVVFMQGCPLRCLYCHNPDTWNPK
                     GKVKYQMTPGELLTEVLRYKSFIARGGVTVTGGEPLLQPEFLKEFFRLCQEQGLHTALD
                     TSGFVCTSKAWEVLDYADLVLLDIKTLNPDLHPLLAGVKQDNTLLFLDELERRGIDTWI
                     RHVIVPGYTDNDEWLEALARYVSSYKVVRKVELLPYHTMGTYKYEQLGLDYPLKGVEPL
                     SKERLDNAKAIFSRYKPEN"
ORIGIN
        1 agatcgtcgg cagcgtcaga tgtgtataag agacagaaat agtgaaggat gagaagcttt
       61 tttatcgcaa tttataggga ttttagaact ggtgtagcat atgtgacaga aatatgacga
      121 taattctcct acctttgcgg cggtaaaaat gtggtattag ataagattgt ttatgaaatt
      181 tgagaatgag tgctgggatc actttaaggg tgagacctgg aaaagagaga tcaatgtaag
      241 agattttatc cagagtaact atacgcctta tgaaggggat gattcctttt tagtcgcttc
      301 ttccgagaag acccgtaagg tctggaacaa gctgaccgag atgtttaagg tcgagaggga
      361 aaagggagtt tatgacgccg agacaaaatt acctcaaggg atcgatgtat atggcccggg
      421 ctatattgat aaggagaatg aggtgatcgt tgggttacaa accgatgctc cgctgaaacg
      481 tggtatcttc ccgaaaggcg gtatccgtat ggtcgagaat agcttggaag cttatggtta
      541 tcatttggac ccgatgacga aagagatctt cacgaagtac cgtaagacgc ataacgaggg
      601 cgttttctcc gcatatacgg aagagatgat tgccgcacgt cgttcggcta ttatcaccgg
      661 tctgcccgac gcgtatggta gaggacgtat tattggtgac tatcgtcgtg tcgctctgta
      721 tggtacggca atcttgatcg aggagaaaaa gcgtttcttg aatcgtttgg atatccaaga
      781 gatcacggaa gagatcatcc agagccgtga ggagatttcc gaacagatca aggctttgaa
      841 agcgttcgag aaaatgtgcg ctagctatgg ctttgatgtg actcgtccgg cacaaaacgc
      901 tcgtgaggcg gttcagtttg tgtatttagc ttatctggcg gcggtaaaag accaagatgg
      961 cgcggcgatg tctatcggac gtacttctac gtttttggat atttatatcg agaaagacat
     1021 ccgtgagggt aagttgaccg aagaggaggc gcaagaattg gtagaccagc tgatcatcaa
     1081 gttgcgtatc gttcgtttct tacgtacgcc ggaatacaat gatttgttct ccggtgatcc
     1141 ggtttgggta acagagtcct tgggcggcca aggagtggat ggccgttctt tggtaacaag
     1201 aacttcctat cgttatctcc atacattata taatctaggt ccggcaccag aacccaattt
     1261 gacggttctg tggtttaaga acgctcccga gaactggaaa cgtttctgtg ccaaggtctc
     1321 tatcgatacg tcggctatcc aatatgagaa cgacgatttg atgcgtccgg attatggaga
     1381 tgattatggt atcgcttgtt gcgtttctcc tatgaagatc ggtaagcaga tgcagttctt
     1441 cggagcccgt gcgaatttgg cgaaatgttt attatatgcg atcaacggag gacgggatga
     1501 gcgcagcggc gtacaggtag ccccgatgtt cgagccggtt cgcggcgagt atctggaata
     1561 cgatgaggtg atggctaagt atgagcaaat gatgcgctgg ttggcgaaag tgtatgtgaa
     1621 cgccttgaag ataatccatt atatgcatga taaatacgcg tatgaggcat tcgagatgtc
     1681 gcttcacgat ggggatgtag agcgtatccg tgcgacaggt atcgccggtc tttctatcgt
     1741 tgccgattcg ttggctgcta tccgtgatac gaaagtacgt gtgatccgtg acgagcgtgg
     1801 cttggcggtt gatttcgaac gtgagggcga atatgttcct ttcggtaata acgatgatcg
     1861 tacggatagt atcgctgtag agatcaccga gaagttcatg gaatatttgc gtcagcacga
     1921 gacctatcgc catgcgaaac ctacgcagtc catccttacg atcacctcta atgtggtata
     1981 tggtaagaag actggtacga caccggatgg ccgtatcggt ggaactcctt tcgctccggg
     2041 tgcgaaccct atgaacggtc gtgatacgaa aggtgctatc gctgcgctag cttcggtagc
     2101 gaagcttcca ttccagcatg cgcatgacgg catctcttat actttcgctg tttctccggc
     2161 tacgttggga aaggagcgtg aagtgcagat caataatctg gttagcttat tggatggcta
     2221 ttttactccg gatggaggtc agcatctgaa tgttaacgta tttgataagg atttgttgat
     2281 cgacgctatg gagcatcccg agaaatatcc gcaacttacg atccgtgtat ccggatatgc
     2341 ggtaaacttc gtgaaactga ctcgtgaaca acagttggat gttatttcaa gaaccattaa
     2401 ccattcactg taagtgataa aaggtaagat acattcatta gagagtttcg ggaccgtaga
     2461 tggtcccgga attcgctttg tcgtttttat gcagggctgc ccgttgcgtt gcctgtattg
     2521 ccataacccg gatacttgga acccgaaagg gaaggtgaaa tatcagatga ctcccggcga
     2581 gctattgacg gaagtcttac ggtataagag ttttatcgcc cgtggggggg tgactgttac
     2641 cggtggggaa cctttattgc aacctgagtt tctgaaagag tttttccgtt tgtgccaaga
     2701 gcaaggtctt cacacagctt tggacacatc tggtttcgta tgtacctcaa aagcttggga
     2761 agtcttggat tatgcggact tagtgttatt ggacatcaaa accttaaacc cggatttaca
     2821 tccgttactg gcgggtgtca agcaggataa tacgcttctg ttcttggatg aattggagag
     2881 acgtggcata gatacttgga ttcgccatgt tattgtcccc ggatatacgg ataatgacga
     2941 gtggctggag gcgttagctc ggtatgtcag ttcatataaa gtggttcgca aagtggaatt
     3001 attgccttat catacgatgg gaacctataa atatgagcaa ttaggcctcg attatccgtt
     3061 aaagggggtg gaacccttat caaaagaacg tttagataat gcgaaagcga tattctctcg
     3121 ctacaaacca gaaaat
//
