LOCUS       c00209_gnlPro..         6904 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|EFNNFLMH_209.
ACCESSION   c00209_gnlPro..
VERSION     c00209_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-07 17:48:48
            Original ID  :: gnl|Prokka|EFNNFLMH_209
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 6904
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..6904
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[3019:5995]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      3020..5995
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..6904
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..6904
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             complement(3..389)
                     /locus_tag="ctg209_1"
                     /transl_table=11
                     /translation="MRFTRLILALTSFLILITQTDLFASTVLHKTSMVDLSNNAIICMH
                     QDPDGYLWIGTYDGLNLYNGKDTYVYRFELNNKNSLCSNIIHKISDAEPGFLWISTSLG
                     INKFSLKERKVTESFPGYMECDLLA"
     CDS             546..2849
                     /locus_tag="ctg209_2"
                     /transl_table=11
                     /translation="MEQCEENSQENDDVIIEKEISKLIEEQRLFTESIYACLPVGIEIY
                     DAHGVLRSINDHALRMYGVDDRTSVVNIVNLYKSPFVDSELLARIQSGEDISLEFEYDF
                     DRVNKDAYYSSHNKDTMIYGVKVIPIWSRKKHLIGHILLANDMTSVKDVEFRTEESKKN
                     LEMAMKAANMASWVYDVNKKVFNPLYGLTVAKKNMPMEAVLDILHPQDRAPLIRLFSQL
                     IGNEIEQGQMTLRFYNEQEEMYRYYESRMRLSTEHRGKLQIIGTQMDITERLQMAKKAQ
                     DLIAKRDLAMKVSNIVHWDFDVNSQKFESYNDPINDYVSDRLLTVSEYMDVIYPEDRSV
                     FYDAMQSLIAGKNVTINFTCRIQTKYDETWQYCDFMGVPFDQDENGDIIRYTGFRQNIP
                     KIQQLNRELKERNYKIELSFKTVGMSYWDFDVKSLKFSAFNDPINDFYSEKTITPEDYL
                     NATHPDDVVFVRKYMEHMFRGIDKDFNVKYRSKTKWDDEWQTLLVTGIQVERDKEGHVT
                     RYTGITINNTKWEKMIQQLKELKEKAELSDRLKSAFLANMSHEIRTPLNAIVGFSELMV
                     TCDDPEEKKEYINIIQSNNELLLRLINDILDLSKIESGIIERRKENFNLAKVCNELFVT
                     IQAKMTNPNVELRLDGPNSECWVLLDRNRLKQVWMNFLTNAVKCTKSGYIKMGYGIERE
                     GLRIYVEDTGIGIPDDLHDKVFTRFEKLNEFSQGTGLGLTISRAIVEAAGGEVGFTSKL
                     GVGSTFWAWLPLRP"
     CDS             3020..5260
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate2"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg209_3"
                     /sec_met_domain="Gly_radical (E-value: 1.9e-46, bitscore:
                     145.5, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate2 (E-value: 1.5e-282, bitscore:
                     926.7, seeds: 33, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKFENECWDHFKGETWKREINVRDFIQSNYTPYEGDDSFLVASSE
                     KTRKVWNKLTEMFKVEREKGVYDAETKLPQGIDVYGPGYIDKENEVIVGLQTDAPLKRG
                     IFPKGGIRMVENSLEAYGYHLDPMTKEIFTKYRKTHNEGVFSAYTEEMIAARRSAIITG
                     LPDAYGRGRIIGDYRRVALYGTAILIEEKKRFLNRLDIQEITEEIIQSREEISEQIKAL
                     KAFEKMCASYGFDVTRPAQNAREAVQFVYLAYLAAVKDQDGAAMSIGRTSTFLDIYIEK
                     DIREGKLTEEEAQELVDQLIIKLRIVRFLRTPEYNDLFSGDPVWVTESLGGQGVDGRSL
                     VTRTSYRYLHTLYNLGPAPEPNLTVLWFKNAPENWKRFCAKVSIDTSAIQYENDDLMRP
                     DYGDDYGIACCVSPMKIGKQMQFFGARANLAKCLLYAINGGRDERSGVQVAPMFEPVRG
                     EYLEYDEVMAKYEQMMRWLAKVYVNALKIIHYMHDKYAYEAFEMSLHDGDVERIRATGI
                     AGLSIVADSLAAIRDTKVRVIRDERGLAVDFEREGEYVPFGNNDDRTDSIAVEITEKFM
                     EYLRQHETYRHAKPTQSILTITSNVVYGKKTGTTPDGRIGGTPFAPGANPMNGRDTKGA
                     IAALASVAKLPFQHAHDGISYTFAVSPATLGKEREVQINNLVSLLDGYFTPDGGQHLNV
                     NVFDKDLLIDAMEHPEKYPQLTIRVSGYAVNFVKLTREQQLDVISRTINHSL"
     CDS             5261..5995
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 67.6;
                     E-value: 4.7e-21)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg209_4"
                     /sec_met_domain="Radical_SAM (E-value: 6.4e-34, bitscore:
                     106.2, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="VIKGKIHSLESFGTVDGPGIRFVVFMQGCPLRCLYCHNPDTWNPK
                     GKVKYQMTPGELLTEVLRYKSFIARGGVTVTGGEPLLQPEFLKEFFRLCQEQGLHTALD
                     TSGFVCTSKAWEVLDYADLVLLDIKTLNPDLHPLLAGVKQDNTLLFLDELERRGIDTWI
                     RHVIVPGYTDNDEWLEALARYVSSYKVVRKVELLPYHTMGTYKYEQLGLDYPLKGVEPL
                     SKERLDNAKAIFSRYKPENNKA"
     CDS             6194..6772
                     /locus_tag="ctg209_5"
                     /transl_table=11
                     /translation="MKTPQEIAEMISKRFYPLPDADLDSLASIIESSKAAKGELLLDNG
                     QISKYLYYVESGLLRQFYYKNKRDITEHFSCEGNIVICIASTFQREPTRLLIEALEPTV
                     YHRIFFSEFSDLLKTSLAINRLYQKILEWALMLSQEKADSWRFESARKRYLRFQQEYPE
                     AAKRASVNHIASYLLMTPESLSRVRAGVL"
ORIGIN
        1 gacgctaata gatcgcattc catgtatccg gggaaagact cggtgacttt ccgttctttt
       61 agtgagaatt tattgatacc taaagaggtt gatatccata aaaagccggg ttctgcgtcg
      121 gatattttgt gaatgatatt actacaaaga gagtttttgt tatttaattc gaaacgatat
      181 acatacgtat cttttccgtt gtataagtta agtccgtcgt aagtcccgat ccacaaatag
      241 ccatccggat cttgatgcat gcaaataata gcgttatttg agagatcaac catagaagtt
      301 ttatgcaata cggttgaggc aaataggtct gtttgcgtga tcaatataag gaaactggtt
      361 aacgctagaa taagccttgt gaatctcatg tatttaagat ttagtgtttg ttttcagcgc
      421 aaagataata tctttatgaa cgaacagaaa agaattcact tttttgttta cttttgcaaa
      481 aagcagggct ttcgtataaa ttagaatgtc tgacgtatga taatacgaaa taaagtaagt
      541 gacagatgga acaatgtgaa gagaattctc aagaaaatga cgatgtgata atagagaaag
      601 aaatttctaa attaatagaa gaacagcgtt tatttacgga gagtatatat gcttgtctgc
      661 cggtgggtat tgaaatttat gacgcacatg gtgtgctgcg tagtataaac gaccatgcgt
      721 tgaggatgta tggtgtggac gaccggacat cggtggtgaa tatagtcaat ctttacaaaa
      781 gtccatttgt tgattcagaa cttttagctc ggatacaatc gggtgaagat atttccttgg
      841 agtttgagta tgattttgat cgggttaata aggatgcata ttattcttcc cataataaag
      901 acacgatgat ctatggggtt aaagttatcc cgatatggag tagaaaaaaa catcttatcg
      961 gacatatatt actcgcaaat gatatgactt ccgtgaaaga tgtggaattc cgtacggaag
     1021 aaagcaagaa aaatctggag atggccatga aagcggctaa tatggcttct tgggtatatg
     1081 atgtgaataa aaaggttttt aatccattat atggactaac cgtagccaaa aaaaatatgc
     1141 caatggaggc ggtactggat attttgcatc cccaagatcg agctccgtta atccggctct
     1201 tctctcaatt gataggaaat gagatagaac aaggacaaat gactttgcgt ttctataacg
     1261 agcaagaaga gatgtatcgt tattatgaga gccggatgcg cctatcgaca gaacatcgtg
     1321 gaaaattgca gatcatcggg acacaaatgg atataaccga aaggttgcaa atggccaaga
     1381 aagcgcagga tctgatcgca aaacgtgatc tggccatgaa agtcagtaat attgttcatt
     1441 gggattttga tgtaaattcc cagaaattcg aatcttataa tgatccgatc aacgattacg
     1501 tttcagatcg gttattgacc gtttcggagt atatggatgt tatatacccc gaggaccggt
     1561 ctgtttttta cgacgcaatg caatccctga tagctggaaa gaatgtcacc atcaatttta
     1621 cctgtcgcat acaaaccaag tatgatgaga cttggcagta ttgtgatttt atgggtgtcc
     1681 ctttcgatca agatgaaaat ggagatataa tacgctatac gggattcagg caaaacattc
     1741 ctaagatcca gcaattgaat cgggaactta aagaaagaaa ttacaagata gagctttctt
     1801 tcaagacggt tggtatgtcg tattgggatt ttgatgtaaa aagcctgaaa ttcagtgctt
     1861 ttaatgatcc catcaatgat ttttattcgg aaaagacaat cactcccgag gattatttga
     1921 acgctacaca tccggatgat gtggtttttg tgcggaaata tatggagcat atgttccggg
     1981 gcatagataa agatttcaac gttaaatatc gttccaaaac caaatgggat gacgagtggc
     2041 agaccttatt ggttactgga atccaggttg aaagagataa agaaggccat gtgactcgct
     2101 ataccggtat tacgatcaat aatacgaagt gggagaagat gattcagcaa ttgaaggaat
     2161 taaaggagaa agcggaactt tcagatcgtc tcaagtctgc gtttctcgct aatatgagtc
     2221 acgagatacg taccccgtta aacgcgattg ttggtttttc agagttaatg gtaacttgtg
     2281 atgatccgga agaaaaaaaa gaatatataa atatcatcca atccaataat gagttgttat
     2341 tgcgattgat caacgatatc cttgatcttt ccaagataga gtcaggcata attgaacgta
     2401 ggaaggagaa tttcaatctg gccaaggttt gtaatgagtt atttgttacg attcaggcga
     2461 agatgacaaa tccgaatgtc gagttacgtt tagatggtcc caactcggag tgctgggtac
     2521 ttctggatag aaaccggctt aagcaagtat ggatgaattt cctcactaat gcggtaaaat
     2581 gcaccaaatc cggatatatt aaaatgggat atggcattga aagggaaggt cttcgtatct
     2641 atgttgagga tacgggaatc ggtataccgg atgatttgca tgacaaagta tttacccgtt
     2701 ttgagaaatt gaatgagttc tcgcagggta ccggacttgg cttgacgatc tccagagcta
     2761 tcgtagaagc ggcaggggga gaagtgggct tcacttcaaa acttggtgtc ggatctactt
     2821 tttgggcatg gttgcctctt cgcccttagg tctgactccc catttattgg taatgtcctt
     2881 ttaaaatagt gaaggatgag aagctttttt atcgcaattt atagggattt tagaactggt
     2941 gtagcatatg tgacagaaat atgacgataa ttctcctacc tttgcggcgg taaaaatgtg
     3001 gtattagata agattgttta tgaaatttga gaatgagtgc tgggatcact ttaagggtga
     3061 gacctggaaa agagagatca atgtaagaga ttttatccag agtaactata cgccttatga
     3121 aggggatgat tcctttttag tcgcttcttc cgagaagacc cgtaaggtct ggaacaagct
     3181 gaccgagatg tttaaggtcg agagggaaaa gggagtttat gacgccgaga caaaattacc
     3241 tcaagggatc gatgtatatg gcccgggcta tattgataag gagaatgagg tgatcgttgg
     3301 gttacaaacc gatgctccgc tgaaacgtgg tatcttcccg aaaggcggta tccgtatggt
     3361 cgagaatagc ttggaagctt atggttatca tttggacccg atgacgaaag agatcttcac
     3421 gaagtaccgt aagacgcata acgagggcgt tttctccgca tatacggaag agatgattgc
     3481 cgcacgtcgt tcggctatta tcaccggtct gcccgacgcg tatggtagag gacgtattat
     3541 tggtgactat cgtcgtgtcg ctctgtatgg tacggcaatc ttgatcgagg agaaaaagcg
     3601 tttcttgaat cgtttggata tccaagagat cacggaagag atcatccaga gccgtgagga
     3661 gatttccgaa cagatcaagg ctttgaaagc gttcgagaaa atgtgcgcta gctatggctt
     3721 tgatgtgact cgtccggcac aaaacgctcg tgaggcggtt cagtttgtgt atttagctta
     3781 tctggcggcg gtaaaagacc aagatggcgc ggcgatgtct atcggacgta cttctacgtt
     3841 tttggatatt tatatcgaga aagacatccg tgagggtaag ttgaccgaag aggaggcgca
     3901 agaattggta gaccagctga tcatcaagtt gcgtatcgtt cgtttcttac gtacgccgga
     3961 atacaatgat ttgttctccg gtgatccggt ttgggtaaca gagtccttgg gcggccaagg
     4021 agtggatggc cgttctttgg taacaagaac ttcctatcgt tatctccata cattatataa
     4081 tctaggtccg gcaccagaac ccaatttgac ggttctgtgg tttaagaacg ctcccgagaa
     4141 ctggaaacgt ttctgtgcca aggtctctat cgatacgtcg gctatccaat atgagaacga
     4201 cgatttgatg cgtccggatt atggagatga ttatggtatc gcttgttgcg tttctcctat
     4261 gaagatcggt aagcagatgc agttcttcgg agcccgtgcg aatttggcga aatgtttatt
     4321 atatgcgatc aacggaggac gggatgagcg cagcggcgta caggtagccc cgatgttcga
     4381 gccggttcgc ggcgagtatc tggaatacga tgaggtgatg gctaagtatg agcaaatgat
     4441 gcgctggttg gcgaaagtgt atgtgaacgc cttgaagata atccattata tgcatgataa
     4501 atacgcgtat gaggcattcg agatgtcgct tcacgatggg gatgtagagc gtatccgtgc
     4561 gacaggtatc gccggtcttt ctatcgttgc cgattcgttg gctgctatcc gtgatacgaa
     4621 agtacgtgtg atccgtgacg agcgtggctt ggcggttgat ttcgaacgtg agggcgaata
     4681 tgttcctttc ggtaataacg atgatcgtac ggatagtatc gctgtagaga tcaccgagaa
     4741 gttcatggaa tatttgcgtc agcacgagac ctatcgccat gcgaaaccta cgcagtccat
     4801 ccttacgatc acctctaatg tggtatatgg taagaagact ggtacgacac cggatggccg
     4861 tatcggtgga actcctttcg ctccgggtgc gaaccctatg aacggtcgtg atacgaaagg
     4921 tgctatcgct gcgctagctt cggtagcgaa gcttccattc cagcatgcgc atgacggcat
     4981 ctcttatact ttcgctgttt ctccggctac gttgggaaag gagcgtgaag tgcagatcaa
     5041 taatctggtt agcttattgg atggctattt tactccggat ggaggtcagc atctgaatgt
     5101 taacgtattt gataaggatt tgttgatcga cgctatggag catcccgaga aatatccgca
     5161 acttacgatc cgtgtatccg gatatgcggt aaacttcgtg aaactgactc gtgaacaaca
     5221 gttggatgtt atttcaagaa ccattaacca ttcactgtaa gtgataaaag gtaagataca
     5281 ttcattagag agtttcggga ccgtagatgg tcccggaatt cgctttgtcg tttttatgca
     5341 gggctgcccg ttgcgttgcc tgtattgcca taacccggat acttggaacc cgaaagggaa
     5401 ggtgaaatat cagatgactc ccggcgagct attgacggaa gtcttacggt ataagagttt
     5461 tatcgcccgt gggggggtga ctgttaccgg tggggaacct ttattgcaac ctgagtttct
     5521 gaaagagttt ttccgtttgt gccaagagca aggtcttcac acagctttgg acacatctgg
     5581 tttcgtatgt acctcaaaag cttgggaagt cttggattat gcggacttag tgttattgga
     5641 catcaaaacc ttaaacccgg atttacatcc gttactggcg ggtgtcaagc aggataatac
     5701 gcttctgttc ttggatgaat tggagagacg tggcatagat acttggattc gccatgttat
     5761 tgtccccgga tatacggata atgacgagtg gctggaggcg ttagctcggt atgtcagttc
     5821 atataaagtg gttcgcaaag tggaattatt gccttatcat acgatgggaa cctataaata
     5881 tgagcaatta ggcctcgatt atccgttaaa gggggtggaa cccttatcaa aagaacgttt
     5941 agataatgcg aaagcgatat tctctcgcta caaaccagaa aataacaagg cctgatctct
     6001 cgatcaagcc ttgcgattat gcaacagcta atcagggaaa acaatctaat ccgattagct
     6061 tttaaacgat agtaataatt ttttgcctag actaaccttg actatttaat atgtgtttgt
     6121 tttgttatat gcaacaaata tctatttttg taaggagata aaaaatgata tagatcagaa
     6181 aatgatgaat agtatgaaga caccacaaga aatagccgaa atgatatcca aacgttttta
     6241 tccattaccc gatgcggatt tggattctct ggcttcgatc atagaatcaa gtaaggctgc
     6301 gaaaggagaa ttattgttgg ataacggaca aatatctaaa tatttatatt atgtggaatc
     6361 gggattatta cgccagtttt actataagaa taaacgtgat attaccgagc atttctcctg
     6421 tgagggaaat atcgttattt gtatagccag cacattccaa agagaaccga cgcgtttatt
     6481 aatcgaggca ttggaaccga cggtttatca ccggatcttt ttttcggaat ttagcgattt
     6541 attgaaaaca tctttagcta ttaatagatt gtaccagaaa atactggagt gggctttgat
     6601 gctttcgcaa gagaaagcgg actcttggcg ttttgagtcg gcaagaaaac gttatctgcg
     6661 ttttcaacag gaatatccgg aagcagcgaa acgtgcttct gtcaatcata tcgcctcgta
     6721 tttattgatg acacccgaat ctttgagccg ggtgcgggcg ggagtgttat aatcgttact
     6781 gatgcggtaa tccaaaaccc aagatattga agggcttctc cgtgaggata ggcccttttt
     6841 attgattgat aaagtattat ctactgtctc ttatacacat ctccgagccc acgagactcc
     6901 tgag
//
