LOCUS       c00115_gnlPro..         6114 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|AINHGDBO_115.
ACCESSION   c00115_gnlPro..
VERSION     c00115_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-05 17:37:30
            Original ID  :: gnl|Prokka|AINHGDBO_115
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 6114
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..6114
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[2115:5179]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      2116..5179
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..6114
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..6114
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             complement(42..830)
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Lipase_GDSL_2"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg115_1"
                     /sec_met_domain="Lipase_GDSL_2 (E-value: 3.3e-24, bitscore:
                     74.7, seeds: 1564, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNFLSYILAILLGSSSLAFSAPSIPASALPATLKPEPHQRDPYDW
                     NARHEAVKKRNRAVKPEYVMIGDSITHHWGGEPSGDGKNTGSGSWEKLFGPHAATNMGF
                     GFDYVDNAYYRVQNGELDGISPRVIIVLLGTNNLGHRKDTPQACADNIRAFVRLVRRKC
                     PSSKILLLGVLPRKEKNLAEPVKQTNRLLAELHNGKDIFFADPGKALLSADGASPQNEF
                     MKDVVHPNARGYEVLEKELAVLLKKLDAKYRGGKQAGKHS"
     CDS             complement(1053..1835)
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Lipase_GDSL_2"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg115_2"
                     /sec_met_domain="Lipase_GDSL_2 (E-value: 2.5e-24, bitscore:
                     75.1, seeds: 1564, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKSYACLCLLLAGVFCVACFSGACADELPPTLKPQVNPRAVYDWK
                     KRHEAVMERNRTVKPEYVVFGDSITHRWGGEPSGDNRALGTGKTAWDSLFSSHAVTNMG
                     FGSDYVDNAYYRLQLGELDGISPRVIIVLLGTNNLGGRKDAPRVCADNLKAFVSLARRK
                     CPSSKILLLGILPREEKKLAPLIRETNKMISGLADGNSVFFANPGEEFLGEDGVSPKPG
                     LLEDGLHPSARGYDILGKSLSVLLKKMDDKYGKLPAPR"
     CDS             2116..4383
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate2"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg115_3"
                     /sec_met_domain="Gly_radical (E-value: 2.2e-46, bitscore:
                     145.3, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate2 (E-value: 9.1e-282, bitscore:
                     924.1, seeds: 33, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MISIVKDLKESTALPQEWQGFKPGAWTESIDVRDFIQHNYTPYSG
                     NEEFLSGPSQRTLRLWDELKVLLKREIDNGGVLDADEEVVSSITSHKPGYIDKELEVVV
                     GLQTDAPLKRALMPFGGLRMAQQALESYGFKMCEKTADIFKKIRKTHNEGVFDAYTSDI
                     RAARSAGIITGLPDAYGRGRIIGDYRRVALYGTDKLIAERRKDLKNREHSPLTDELIRL
                     REEMSEQIRALEELAQLGASYGCDLTRPAANAREAVQWTYLGYLAAVKEQNGAAMSLGR
                     VSTFFDIYFTRDLEQRLITEEEVQEIIDQFVMKLRIVRFIRTPDYNNLFSGDPTWVTES
                     IGGMGEDGRTLVTRSSFRMLQTLYNLGPAPEPNLTVLWSRNLPEAFKSFCAKVSIETSS
                     VQYENDDLMRPHWGDDYGIACCVSAMRIGKQMQFFGARANLAKCLLYALNGGVDELKGK
                     QVAPPSPRYTEEILNYDEVMALYDKMQDWLAKTYIDALNIIHYMHDKYCYERIEMALHD
                     PEILRTMATGIAGLSVAADSLSAIKYATVKAIRNEEGLIVDFKTEGEFPCYGNNDPRVD
                     DIACSLVSNFMEKLRRLHTYRNSLPTQSILTITSNVVYGKKTGNTPDGRRAGEPFAPGA
                     NPMHGRDRNGAVASMLSVAKLSYDDSLDGISYTFSIVPQALGKEERERRVKLVSLLDAY
                     FAATGHHINVNVLERETLLDAMDHPEKYPQLTIRVSGYAVNFIKLTREQQQEVINRTFH
                     TR"
     CDS             4400..5179
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 54.5;
                     E-value: 4.9e-17)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg115_4"
                     /sec_met_domain="Radical_SAM (E-value: 7e-32, bitscore:
                     99.5, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPQVFPQNHDPDGESSVTGLVHSVESCGTVDGPGIRFVLFLSGCS
                     LRCRYCHNPDTSYVRRGQLRSADDVLKEIARYRDFLQAAGGGVTLSGGDPLFQPDFAGA
                     VLKGCRKLGLHTCLDTSGHLGVNAGEEMLADTDLVLLDIKAWNPKRYRALTGGELRPTL
                     EFAERLASLRKPVWLRYVLVPGVTDNLEEIEELSRHAALLGNVERVDVLPFHQLGRFKW
                     DELKLDYTLKDVPEASAELAEQVRGIFRRGGFPDCVH"
     CDS             complement(5428..6114)
                     /locus_tag="ctg115_5"
                     /transl_table=11
                     /translation="ETLKALEPYGPMMFSGLDTHPGARKYKFRVKAPVLRAAPPAHRVQ
                     LYLQLGVLHGLDVATPSLFPVKKAAPAEDAPILIAPFSRLGSASEWSREQWRELVSLLP
                     GRTVLAALEEDRERAASLAEHLNVELAVGAPEALFPVMDGAAAAVAVDGDFPSLCSFRG
                     LPVVTLFSTRLPDVYRPMGTFNRSLYSHQCCSPCFRKECDRDVPCNRHIAVRDVLEALR
                     EISGKE"
ORIGIN
        1 tcccttcaag caaaatccta ccttccatca gaaaacgatc atcaggaatg ctttcccgcc
       61 tgcttccctc cccggtattt tgcatcaagc tttttcagga gtacggccag ttccttttcc
      121 aaaacctcgt accccctggc attgggatgc accacatcct tcatgaattc attctgcgga
      181 gaagcaccat ctgcggaaag aagcgccttt cccggatcgg caaagaaaat gtcctttccg
      241 ttatgcagtt ccgccaacag tctgttcgtc tgcttcaccg gttccgccag gtttttctcc
      301 ttccgtggga gaacgcccag caacaggatt ttggaagagg ggcacttccg gcgcaccagg
      361 cggacaaatg ccctgatatt gtccgcacat gcctgcggcg tatccttgcg gtgccccaga
      421 ttattggtcc ccagcaacac aatgatgacc cgcggagaaa tgccgtccag ctccccgttt
      481 tggacacggt aataggcatt atccacataa tcaaatccaa agcccatatt cgtcgctgca
      541 tgcggaccga ataatttctc ccaggaaccc gatcccgtat tttttccgtc cccggaaggc
      601 tcccctcccc aatggtgggt aatgctgtcc cctatcatca cgtattcagg cttgacggcc
      661 cggtttcttt ttttaacggc ttcatgccgg gcattccaat catacgggtc ccgctgatgg
      721 ggctccggct tcagggtagc gggcaacgcg gaggccggaa tggaaggcgc agaaaatgcc
      781 agactggaac tccccagcaa tatggcgagt atgtaggaga ggaaattcat aatcaaaaag
      841 taaaaccggt tttcagttta agcaaatcct gccgtgcatc aagatcgcag gcgtcacttc
      901 cgccggcaga gtaaaaaaga ccactgcccg tccggcaaat cctgccgcaa ccacggccag
      961 gcgagcaccg tgttcggcct cttccaaggc gcgctccatt tcatccatcc cccggcctct
     1021 cccggaacag gaacgccctc ccccgtattc atctaccggg gagcgggcag cttgccgtac
     1081 ttgtcgtcca ttttcttcag caatacgctc aacgacttcc ccagtatgtc gtaccccctg
     1141 gcactgggat gcagcccatc ctccagtagc ccgggttttg gggacactcc gtcctccccc
     1201 aggaattcct cgccgggatt ggcgaaaaac acgctgtttc cgtcagccag gccggatatc
     1261 attttattgg tttccctaat caggggagcc agcttttttt cctccctggg gagaatgccc
     1321 agcaacagga ttttggaaga ggggcacttc cggcgcgcca ggctcacaaa agccttgaga
     1381 ttgtctgcgc agacccgggg agcatccttc cgcccgccaa gattattggt tcccagcagc
     1441 acgatgatga cccgcggaga aatgccgtcc agctctccca gctgcaagcg gtaataggca
     1501 ttatccacgt aatcggaacc aaaccccata ttggttaccg catgggaaga aaacaggcta
     1561 tcccaggcgg tttttcctgt tccgagagcc ctgttgtccc cggaaggctc gccgccccag
     1621 cggtgggtaa tgctgtcacc aaagacgaca tattccggtt tgacggtccg gttccgctcc
     1681 atcactgctt catgacgttt tttccagtca tacacggccc gcgggttcac ctggggcttc
     1741 agcgtgggcg gcaattcatc agcacaggcc ccggagaaac aagcaacgca aaaaactccc
     1801 gccagcaata aacaaaggca tgcataagat ttcataacgg taagcgttga gatactgcaa
     1861 tagagattag gaaagtaaag agaaataatc ttccgtcact tttccgcaat cgcatgttcc
     1921 cctcattttc cgctccgcaa cggacagaca ggtgaaacat gccaaagcca gcgcacccgc
     1981 gttctcttcg caaagaatta gccttgttgt cccccggaat tcacgatata attacgcggc
     2041 gcggagtttt gccgtttcca gcctttcagg ggctgcggaa cgtttttctc cagtcttctt
     2101 cactacattc tatttatgat cagcatagta aaagacttga aggagtccac cgcccttccg
     2161 caggaatggc agggattcaa gccgggagca tggacggagt ccattgacgt acgggatttc
     2221 atccagcata actacacgcc ctattccggc aatgaagagt ttctttccgg cccctcccag
     2281 cgcacgcttc gtttatggga cgagctgaaa gtcctgctga aacgggaaat agacaacggc
     2341 ggcgtgctgg atgctgatga agaagtggta tcctccatca catcccacaa gcccggctac
     2401 attgacaagg aactggaagt ggtcgtaggc ctccagacgg acgcccccct gaaacgggcc
     2461 ctgatgccct ttggcggctt gcgcatggcc caacaggccc tggaatccta cgggttcaag
     2521 atgtgcgaga aaaccgcaga cattttcaaa aagatacgca agacgcacaa tgaaggcgtt
     2581 ttcgacgcct acacttccga tatccgggcg gcccgttccg ccgggattat taccggcctt
     2641 ccggacgcct atggccgcgg ccgcatcatc ggggactacc gccgggtggc cctgtacggc
     2701 acggataagc tgattgccga acgccgcaag gacctcaaaa accgtgaaca ttcccccctt
     2761 acggatgagc tgatccgtct gcgcgaagaa atgagcgaac aaatacgcgc cctggaagaa
     2821 ctcgcccagc tgggcgcctc ctacggctgc gacctcactc gccccgccgc aaacgcccgc
     2881 gaagccgtgc aatggacgta cctgggctac ctggccgccg tgaaggaaca gaacggagcg
     2941 gccatgtccc tgggccgcgt ttccactttc ttcgacattt atttcacccg cgacctggaa
     3001 cagaggctca tcacggaaga ggaagttcag gaaatcatcg accagttcgt gatgaagctg
     3061 cgcatcgtcc gtttcatccg cacgcctgat tacaataacc tgttctccgg agaccccacc
     3121 tgggtgacgg aatccatcgg cggcatggga gaggacgggc gcaccctcgt cacccgcagt
     3181 tccttccgca tgctgcagac cctgtacaac ctggggccgg ctccggaacc gaacctgacc
     3241 gttctgtggt cccggaacct gccggaagcc ttcaaaagct tctgcgccaa ggtttccatt
     3301 gaaacgtcct ccgtccaata cgagaacgac gacctgatgc gcccgcattg gggcgacgac
     3361 tacggcatcg catgctgcgt gtccgccatg cgcatcggca agcagatgca gttcttcgga
     3421 gcgcgcgcca acctcgccaa gtgccttctc tacgccctga acggcggcgt ggatgaactc
     3481 aagggcaagc aggtggcccc gccctccccc cgctacacgg aggaaattct caactatgat
     3541 gaggtgatgg ccctgtacga caagatgcag gactggctcg ccaaaactta cattgacgcg
     3601 ctgaacatca tccactacat gcacgacaaa tactgctatg aacgcattga aatggcgctg
     3661 catgatccgg aaattctgcg cacgatggct acgggaatcg ccgggctttc cgtggcggcg
     3721 gactccctgt ccgccatcaa gtatgccacc gtaaaagcca tccgcaatga agaagggctg
     3781 attgtggact tcaagacgga aggggaattc ccctgttacg ggaacaatga cccgcgcgtg
     3841 gacgacatag catgcagcct ggtcagcaat ttcatggaaa aactgcgcag gctccacact
     3901 taccgcaatt ccctgcccac ccagtccatc ctgacgatca cctccaacgt ggtgtacggc
     3961 aaaaagacgg gcaacacgcc ggacggccgc cgagccgggg aaccgttcgc ccccggagcc
     4021 aaccccatgc acggaaggga caggaacgga gccgtggcct ccatgctctc cgtagccaag
     4081 ctgtcctatg acgactccct ggacggcatc tcctacacct tctccatcgt tcctcaggcc
     4141 ttgggcaagg aggaacgtga acgccgcgtc aagctcgtct ccctgctgga cgcctacttt
     4201 gccgctacag gtcaccacat taatgtgaac gtactggaac gggaaacgct tctcgacgcc
     4261 atggatcacc cggaaaaata cccgcagctt accatccgcg tttccggcta tgccgtgaat
     4321 ttcatcaagc tgacccggga acagcagcag gaggtcatca accgcacttt ccacacccgt
     4381 taacccggac tgcaagcgga tgccgcaggt ttttccacaa aaccatgatc cggacggaga
     4441 aagctccgtc accggtttgg ttcattccgt ggaatcctgc ggcaccgtgg acggccccgg
     4501 catccgcttc gtccttttcc tgtccggctg cagcctgcgt tgccgctact gccataatcc
     4561 ggatacctcc tacgtgcgga ggggccagct caggagcgca gatgatgtcc tcaaggaaat
     4621 agcccgttac cgggattttt tgcaggcggc gggcggcgga gtcaccctct ccggcgggga
     4681 tcccctcttc cagccggatt ttgccggtgc cgtgctgaaa ggctgccgga aactgggcct
     4741 gcacacctgc ctggacacct ccggccatct gggcgtgaat gcgggcgaag aaatgctggc
     4801 ggatacggac ctggtcctgc tggatatcaa ggcatggaat ccgaaacggt atcgcgccct
     4861 gacaggcgga gaactgcgcc ccacactgga gtttgcggaa cgcctggcct ccctccgcaa
     4921 gcccgtgtgg ctgaggtatg tgctggtacc tggcgttacg gacaatctgg aagaaattga
     4981 ggaactctcc cgccatgcgg cgcttctggg caacgtggaa cgcgtggacg tgctcccttt
     5041 ccaccagctg gggagattca aatgggatga attgaagctg gactatactt tgaaggacgt
     5101 cccggaagcg tccgcagaac tggcggaaca ggtccgcggc attttccggc ggggaggctt
     5161 tccggattgc gtgcattaac cggattttcg cgctttggcg ccaccaaccg cagcaaaccg
     5221 gacgcaggca tccccctctt ccggatgagg ggccctctcc cctccactgc agaatccatg
     5281 caaaaaggaa gacgcatttc tggaattgct ccggaatacg gcatttccgc aattcctttc
     5341 gttatttcat cattctttac ggcaacggca gcattcccgc cgttttttcc gccaacgccg
     5401 ggggacggca ttccgtccct tcccagatta ttctttcccg gaaatttccc gcaaggcttc
     5461 cagtacgtcc cgcacggcaa tgtggcggtt gcagggaaca tcacggtcac attccttccg
     5521 gaagcaggga gaacagcact ggtggctgta aagggaacgg ttgaatgtgc ccatagggcg
     5581 gtaaacatcc ggaagccggg tggaaaacag agtcaccacc ggaagccccc ggaaagaaca
     5641 gagggacgga aagtcgccat ccacggctac tgccgccgca gcgccgtcca tgacgggaaa
     5701 gagagcctcc ggcgcaccca ccgccagctc cacattcaaa tgttccgcca gggaggcggc
     5761 acgctcccgg tcttcttcca gagccgccag caccgtccgt cccggcagaa gggaaaccag
     5821 ctccctccac tgctccctgc tccattcact ggcgctcccc aggcgggaaa acggagcgat
     5881 taaaatgggg gcgtcttccg ccggggccgc ttttttcaca gggaacaaag acggggtggc
     5941 gacgtccagc ccatgcagca cccccaattg cagataaagc tgcacccggt gcgccggggg
     6001 ggcggcgcgc aggacggggg cctttacccg gaacttgtac ttccgggcac cgggatgcgt
     6061 gtccagcccg gaaaacatca tggggccata cggttccagc gctttcaggg tttc
//
