LOCUS       c00277_gnlPro..         5067 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|GHLGEGDM_277.
ACCESSION   c00277_gnlPro..
VERSION     c00277_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-07 18:03:13
            Original ID  :: gnl|Prokka|GHLGEGDM_277
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 5067
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     CDS             complement(1..1542)
                     /locus_tag="ctg277_1"
                     /transl_table=11
                     /translation="MKHFTIFQGFYYAIAEMTEEEIVSTIGSFTYREKVEEIRRIFAEQ
                     GEKAANEKKKELPAIAFSASYRGRRTKVNLVKYLGHIVIDIDHLSKEELARILPIIKRC
                     DYTRIAFISPKGMGVKIIVRACHPDETLPETLQEIEDFHHAAYTRLVSFYTELCQIEID
                     TSGQDVARTCLFSYDPEIYFNPNADAFLVDQPQASYKVSNRKNLSGSKQQTPPDGPPTN
                     EDTALNAHSANASLVLTLTYYHNKSEKYIVGNRNNYLHHLSCTFNRYGIPQEESSAFIK
                     SQFTDFPADETASLINSAYAHTDEFNTCKLNGTQKRILRIEQYISEHYETRYNEVLHIM
                     EYRRRHPDTEKPEPFRILDEMMENSIWIEINELGYACTVKTIENLIYSDFSQSYHPIRE
                     YFELLPEWDGTDYIRILADSVQTSHQEFWAECLERYLVAMCAAATQENIVNHTVLLLCS
                     EIQNIGKTTFINNLLPPELRAYLSTGLINSNNKDDLAKITQAMLINLDEFEGMSGR"
     protocluster    1..5067
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[3211:5066]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      3212..5066
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..5067
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..5067
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             complement(1629..1859)
                     /locus_tag="ctg277_2"
                     /transl_table=11
                     /translation="MNYKEDSFLIRTYTKAELAHLYNPHVCLKVALQILRRWIIYNLPL
                     LHELEQEGYRARNRLLSPKQVATIIHFLGEP"
     CDS             2053..2556
                     /locus_tag="ctg277_3"
                     /transl_table=11
                     /translation="MAMTVSYSVVPRKNPAKKSEPAKYYAQAQASGELDFEELCEAITS
                     RSTCTETDVRAAISGILYEVKRALKAGRIARLGDLGSLQIGLNSEGAASVKEFSGSMIK
                     GAHLIFRPGKTLAELMKILSYQQVQTRAVAQAGAGDGGGEDPDKNPDSGGDGSGDEEAP
                     DPTV"
     CDS             2616..2783
                     /locus_tag="ctg277_4"
                     /transl_table=11
                     /translation="MKEIVSRILDVIMYLIPFFGKRKRDRVVREVRYHTTCKEVCKVKT
                     TEREKEHEKD"
     CDS             2770..3219
                     /locus_tag="ctg277_5"
                     /transl_table=11
                     /translation="MRKISLIVIHCSATRVDCDFTAKDVDTAHRYRGFSCWGYHYYIRK
                     SGEIEPMRDKDTVGAHARGYNAISLGVCYEGGLDENGKAADTRTPRQKEALHRLVHELL
                     QRYPEAKVVGHRDLSPDTNYNGIVDPWERIKECPCFEVIGEFSIN"
     CDS             complement(3212..3940)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 72.5;
                     E-value: 1.4e-22)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg277_6"
                     /sec_met_domain="Radical_SAM (E-value: 1.3e-29, bitscore:
                     92.6, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MMINVHSYESMGTFDGPGLRLVVFLQGCNFRCLYCANPDTIAGKG
                     GTPTPPEEIVRMAMSQRPFFGKRGGITFSGGEPTFQAKALVPLVRELKEKGIHVCLDSN
                     GGLWNEDVEELFRLTDLVLLDIKEFNPNRHQTLTGRSNEQTIRTAAWLEEQEKPFWLRY
                     VLVPGYSDFEEDIRALGEALGKYKMIQRVEILPYHTLGVHKYEAMGQEYKMKGVKENTP
                     EQLEKAAEVFKEYFTTVVVN"
     CDS             complement(3954..5066)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate1"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg277_7"
                     /sec_met_domain="Gly_radical (E-value: 1.4e-47, bitscore:
                     149.7, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate1 (E-value: 1.3e-137, bitscore:
                     448.5, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="EFCAKVSIDTSSIQYENDDLMREVRQSDDYGIACCVSYQEIGKQI
                     QFFGARCNLAKALLLAINGGRCENTGTVMVKNIPVLTSDTLKFEEVMDNYKKVLTEIAR
                     VYNEAMNIIHYMHDKYYYEKAQMALVDTNPRINLAYGVAGLSIALDSLSAIKYAKVTAR
                     RNDIGLTEGFDIEGEFPCFGNDNDKVDHLGVDLVYFFSEELKKLPVYKNARPTLSLLTI
                     TSNVMYGKKTGATPDGRAKGVAFAPGANPMHGRDKNGAIASLSSVAKLRYRDSQDGISN
                     TFSIVPKSLGATDEDRIENLVTMMDGYFTKGAHHLNVNVLNRDMLYDAMEHPENYPQLT
                     IRVSGYAVNFVKLSREHQLEVISRSFHERM"
ORIGIN
        1 ccgaccgctc atcccttcaa actcgtcgag attgatcagc atggcctgcg ttattttggc
       61 taaatcatct ttattgttcg aattgattag tccggtagag agataagcac gcagttcggg
      121 aggcaggaga ttattgataa aggtagtctt tcctatgttc tgtatctcgc tgcatagcag
      181 aagtaccgtg tgattgacaa tattctcttg agtagccgct gcgcacatcg ccaccagata
      241 acgttccagg cattccgccc aaaactcctg atgactcgtc tggacactat ccgccaggat
      301 acggatatag tcggttccgt cccattcggg cagtaattca aaatactcgc gaatgggatg
      361 atacgactgg ctgaaatcgg agtaaatcag attttcgatt gttttgacag tgcaggcata
      421 tcccagttcg ttgatttcta tccagatact gttttccatc atctcatcca agatgcggaa
      481 aggctccggt ttctcggtgt cgggatgcct tctgcgatat tccatgatat gcagcacttc
      541 gttgtagcgt gtttcgtagt gctcgctgat atattgctct atccgtaata ttcgtttctg
      601 cgtgccattt agcttacagg tattaaattc gtcggtatga gcgtaggcgc tattgatcaa
      661 agaggctgtc tcgtccgccg gaaagtctgt aaactggctt ttgataaagg cggatgattc
      721 ctcctgcgga atgccgtagc gattgaaggt acatgagaga tggtgcagat aattgttccg
      781 gttgcccact atgtattttt cggacttgtt gtgatagtag gttaaagtca gcaccagaga
      841 agcgttggcg gaatgggcgt tgagcgctgt gtcttcattc gtcggggggc cgtccggagg
      901 agtctgttgt ttactgccgg agagattttt acgatttgaa accttgtagg atgcctgagg
      961 ttgatctaca agaaaagcgt cagcgttcgg attgaaataa atctccggat cgtaagagaa
     1021 cagacaagtg cgtgccacat cctgaccgga ggtgtcgatt tctatctgac agagttcggt
     1081 gtaaaaagaa acgagtctgg tgtaagctgc atgatggaag tcttctattt cctgaagggt
     1141 ttcgggcaat gtctcatccg gatggcatgc gcgtactatg attttcacgc ccatcccttt
     1201 ggggctgata aaggcgatgc gggtgtaatc gcatctcttg atgataggga ggatgcgtgc
     1261 caattcttct ttgctgaggt ggtcgatgtc tatcacgatg tgtcccagat atttgacgag
     1321 gttcactttg gtacgccttc ctcggtagct ggcggagaag gcgattgccg gcaattcttt
     1381 tttcttctca ttggcagcct tttctccttg ctctgcaaag atgcggcgta tttcttcgac
     1441 tttttcccgg taggtaaaac tgccgatagt ggacactatt tcttcttcgg tcatctctgc
     1501 tattgcgtag tagaaacctt gaaatatggt gaaatgtttc atttttattt tgattattgt
     1561 ttgtcgatgc aaaggaactt ctttgcggaa atgtgtaaag gaactttgta taaaatggca
     1621 ataagttact acggttcgcc caaaaagtgt atgatagttg ccacttgttt cggcgaaagg
     1681 agacggttgc gtgcgcggta gccttcctgt tcgagttcgt gcaggagagg gaggttgtaa
     1741 ataatccaac gacgaagtat ttgtaaagca actttgaggc agacatgggg attgtaaagg
     1801 tgagccagtt ccgctttggt ataggtgcgg ataaggaaag aatcttcttt gtaattcatg
     1861 atgttttctt cttgttttat gctataaagt tacataaaat tccttgttat tcaaaagttt
     1921 gacttcgttt ttattcgttg ggaatcaaga aacttcgttg gggaatatgg cttgtccggc
     1981 tatccttatc tttgtgctgt taacaagaag gggatgcatc ctatgtatta taaactttaa
     2041 aattatttta ttatggcaat gacagtatct tattcagtgg tgccgcgcaa gaatcctgca
     2101 aaaaagagcg aaccggcaaa gtattatgca caggcgcagg cgtcgggaga gttggacttt
     2161 gaagagttgt gcgaagcgat caccagtcgt tctacttgta cggaaacgga tgtacgtgcg
     2221 gctatttcgg gtattcttta cgaagtgaag cgtgcgttga aggcaggaag aattgcgaga
     2281 ctgggcgatt tgggcagttt gcagatagga ctcaacagtg aaggtgcggc atcggtcaag
     2341 gaattttccg gttcaatgat taagggggcg catcttattt tccgcccagg taagacgctg
     2401 gcggagttga tgaagattct gagttatcag caagtgcaga ctcgtgcggt ggcgcaggcg
     2461 ggtgcaggcg atggcggggg agaagaccct gacaagaatc cggattccgg tggtgatggc
     2521 tccggtgatg aagaggctcc ggaccctacg gtataattgg cagacgggtc aaggtaataa
     2581 cgaattagaa taacttccat taatattttg ataagatgaa agaaatagta agtcggattt
     2641 tagatgtgat catgtatctg attcctttct tcggaaagag aaagcgggac agggtggtga
     2701 gagaagtgcg ctatcatact acttgtaagg aggtgtgcaa agtgaaaacg acggaaaggg
     2761 aaaaggaaca tgagaaagat tagtctgatt gtgatccatt gttccgccac ccgtgtcgat
     2821 tgcgacttta cggcaaagga tgtggatacg gctcaccgtt atcgtggctt ttcgtgctgg
     2881 gggtatcatt attatatacg caagtcgggc gagattgagc cgatgcggga taaagatacg
     2941 gtcggagctc atgcacgtgg ctataatgcg atcagcttag gtgtatgtta tgagggcggt
     3001 ctggatgaga atggaaaagc tgcggatacg cgtacacccc gtcagaagga ggcgctacat
     3061 cgcttggtac acgaattgtt gcagcgttat ccggaagcta aagtagtagg gcatcgggat
     3121 ttaagtcccg acaccaacta taatgggatc gtagatcctt gggaacggat taaagaatgc
     3181 ccttgctttg aggtcattgg agaattctcc attaattaac taccaccgta gtgaaatact
     3241 ctttgaatac ctccgcagct ttttcaagct gttcgggggt attttctttt acccctttca
     3301 tcttgtattc ctgtcccatc gcttcgtact tatgtacgcc cagtgtatgg taaggcagta
     3361 tttccacccg ttggatcatc ttgtattttc cgagtgcttc tcccagtgcg cgtatgtctt
     3421 cttcaaaatc gctgtatccg ggcactagca cgtagcgtag ccagaaaggt ttctcctgtt
     3481 cttccagcca ggcggccgtg cggatggttt gctcgttgct tcttccggtg agcgtttgat
     3541 ggcggttagg gttgaattcc tttatatcca ataacacaag atcggtcagt ctgaataatt
     3601 cttctacgtc ttcgttccaa agtcctccgt tgctgtccag acagacatgg atgccttttt
     3661 ctttcagttc gcggaccagc gggacgagtg ctttcgcctg aaacgtcggt tcgcctccgg
     3721 agaaagtgat tcctcctcgt ttcccgaaga aggggcgctg gctcattgcc atgcggacga
     3781 tttcttccgg tggggtaggc gtgcctccct ttcctgctat cgtatccgga ttggcgcaat
     3841 agagacaacg gaagttgcat ccttgaagaa aaacgacgag ccgtaagccc ggcccgtcga
     3901 atgttcccat actttcgtat gaatgtacgt ttatcatcat atagagtaat agattacata
     3961 cgttcgtgga agctacggct gataacttcc agttggtgtt cgcggctcag cttcacgaag
     4021 tttacggcat agccggaaac gcggatagtc agctgcggat agttttccgg atgctccatg
     4081 gcatcgtaca gcatatcacg gttcagtacg tttacgttca ggtggtgggc acctttggtg
     4141 aagtaaccgt ccatcatcgt tacgaggttc tcgatacggt cttcgtccgt agcacccagt
     4201 gatttcggaa cgatagagaa ggtgttgctg ataccgtcct gtgagtcacg gtaacgaagt
     4261 ttcgctacag aactcagaga agcgatcgca ccattcttgt cgcgtccgtg catcgggttg
     4321 gcacccggag cgaaggcaac acctttggca cgtccgtcgg gagtagcacc ggtctttttg
     4381 ccgtacatca cattggaagt gatagtcagc aaggagaggg taggacgggc attcttgtaa
     4441 acaggcaatt tcttcaattc ttcgctgaag aagtatacca ggtctacgcc aaggtggtct
     4501 accttgtcgt tgtcgttacc gaagcaaggg aattctcctt cgatgtcgaa gccttctgtc
     4561 agaccgatat cattgcggcg ggcagttact ttcgcatatt tgatggcaga cagtgaatcg
     4621 agggcgatgg aaagtccggc tacaccgtaa gctaggttga tgcgcgggtt cgtatctaca
     4681 agagccattt gggccttctc gtagtaatac ttgtcgtgca tatagtggat gatgttcatc
     4741 gcttcgttat atacgcgggc gatttcggtc agcacttttt tgtagttgtc cattacttct
     4801 tcgaacttca gcgtgtcact ggtcagtacg gggatgttct tcaccatgac tgtaccggta
     4861 ttctcgcaac gtccgccgtt gatggcaagc aacagggctt tggccaggtt gcaacgggca
     4921 ccgaagaact gaatctgctt acctatttcc tggtatgata cgcagcaggc gattccgtag
     4981 tcgtcggact gacgtacttc gcgcatcagg tcgtcatttt cgtactggat agaagaagtg
     5041 tcgatagaaa cctttgcaca gaattct
//
