LOCUS       c00131_gnlPro..         5544 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|BANCDLKN_131.
ACCESSION   c00131_gnlPro..
VERSION     c00131_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-07 18:54:06
            Original ID  :: gnl|Prokka|BANCDLKN_131
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 5544
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..5544
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[1740:4717]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      1741..4717
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..5544
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..5544
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             complement(23..589)
                     /locus_tag="ctg131_1"
                     /transl_table=11
                     /translation="MEPIINSQAPEFKVQAYHNGSFKTVSSDDIKGKWAIFFFYPADFT
                     FVCPTELVDMADKYDQFQAMGVEVYSVSTDSHFVHKAWHDASETIRKIKYPMLADPTGV
                     LSRAFGVMIEEDGMAYRGTFLVNPEGKIKLAEINDNGIGRNADELLRKVEAAQFIAEHP
                     SEVCPAKWKKGGETLKPSIDLVGKI"
     CDS             complement(750..1652)
                     /locus_tag="ctg131_2"
                     /transl_table=11
                     /translation="MEQHHHDHHHHEITSLNKAFIIGISLNLAFVVAEFTAGFWYNSLG
                     LLSDAGHNLGDVASLVLAMLAFRLAKVHPTCSYTYGYKKSTVLVSLLNAVILLVAVGII
                     IAESIGKLLHPRPVEGDAIAWVAGIGVVINALTAWLFMKDKDKDLNVKGAYLHMAADAL
                     VSVGVVVSGIVIAHTGWYIIDPIIGLLIAFVIIVSTWGLLRDSIRLSLDGVPAGTDIDK
                     VEKTIHSVEGVEGVHHLHIWAISTTETALTAHVSVADISEMERIKQEIKHDLQHVGIQH
                     ATLEFETSGFKCDGRCCSD"
     CDS             complement(1741..2469)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 72.3;
                     E-value: 1.5e-22)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg131_3"
                     /sec_met_domain="Radical_SAM (E-value: 3.6e-37, bitscore:
                     116.4, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="LEIKGNIHSLESFGTVDGPGIRFVVFMQGCPLRCLYCHNPDTWDS
                     KGLAKYQMTPEELLAEVLRYKNFIAKGGVTVTGGEPLLQAEFLREFFRLCREAGIHTAL
                     DTSGYICTPKALAVLEYADLVLLDIKTIDPDLHPRLTAVKLDNTLRFLDELEKRGVPVW
                     IRHVIVPGLTDNDEALDKLAEYISHYTVVQKAELLPYHTMGTYKYEAQGLDYKLKEVEP
                     LSAERLANAKEIFKKHGITI"
     CDS             complement(2477..4717)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate2"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg131_4"
                     /sec_met_domain="Gly_radical (E-value: 1.1e-45, bitscore:
                     142.8, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate2 (E-value: 3.4e-285, bitscore:
                     935.1, seeds: 33, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKFENENWAQFKGEIWKREINVRDFIQNNYKPYEGDDSFLKDSSD
                     KTKKVWDKLTEMFKVEREKGVYDAETKYPQGIDTYGPGYIDKENEVIVGLQTDAPLKRG
                     IYPKCGTRMVENSLEAYGYKLDPMTKEIFTKYRKTHNEGVFSAYTDEMLAARRSAIITG
                     LPDAYGRGRIIGDYRRVALYGTEILIAEKKHYLKRLDIQELTEEIIQSREEISEQIKAL
                     KAFEKMCAGYGFDVTKPAKDAREAVQFVYLGYLAAVKDQDGAAMSLGRTSTFLDIYIEK
                     DIREGKLTEEEAQELIDQFIIKLRIVRFLRTPEYNDLFSGDPVWVTESLGGQGVDGRSL
                     VTKTSYRYLHTLYNLGPAPEPNLTVLWFNNAPENWKRFCAKVSIDTSAIQYENDDLMRP
                     DYGDDYGIACCVSPMKIGKQMQYFGARANLAKCLLYAINGGRDERSGVQVAPRFEPITS
                     EYLDYNEVMEKYEQMMRWLAKVYVNALKIIHYMHDKYAYEAFEMALHDGSVERIRATGI
                     AGLSIVADSLAAIRDTKVKVIRDERGLAVGFEREGDYVPFGNNDDRTDSIAVDITEKFM
                     EYLRQHQTYRNATPTQSILTITSNVVYGKKTGATPDGREGGTPFAPGANPMNGRDTKGA
                     VAALASVAKLPFQHAHDGISYTFAVSPATLGKERDIQIANMVGLLDGYFTPDGGQHLNV
                     NVFDKELLLDAMEHPENYPQLTIRVSGYAVNFVKLTREQQLDVISRTINHSL"
ORIGIN
        1 ttgccggcct ccagttttac tattagatct taccgaccag atcgatgctc ggtttcaatg
       61 tttcgccacc ttttttccat ttggcggggc aaacttcgct cggatgttcg gcgatgaact
      121 gggcagcttc tactttacga agcagttcgt cggcgttacg gccgataccg ttatcgttga
      181 tttctgccag tttgatcttt ccttccggat ttaccaggaa tgtaccgcgg taagccatac
      241 cgtcttcttc gatcattaca ccgaatgcac ggctcaatac gcctgtcggg tctgccaaca
      301 tcgggtattt gatcttacga atcgtttcag aagcatcgtg ccatgctttg tgtacgaagt
      361 gtgaatctgt acttacagag taaacttcta cacccattgc ctggaactgg tcatatttgt
      421 cagccatatc taccaactcg gtcggacaaa caaatgtaaa gtcagccggg tagaaaaaga
      481 aaatcgccca tttgcctttg atgtcgtcgc tgcttacagt tttgaaacta ccgttatggt
      541 atgcctgaac tttaaactcg ggtgcttgtg aattgataat aggttccata gaatttttat
      601 tttaatcgtt attactattt tgtttttgtt actggtgcaa agataagttg ccgattcgag
      661 gtaagaacaa aaaatccaat cgaaaggttt tatgcagtca tagacaaaat ctttgagtat
      721 cagtaaatca ggtttataat atacctgttt cagtctgagc aacagcgccc atcgcacttg
      781 aatcccgacg tttcaaattc gagcgtagca tgctggattc ccacatgctg aaggtcgtgt
      841 tttatctcct gtttgatacg ttccatttct gaaatatccg caacggaaac atgggcggta
      901 agtgccgttt cggtcgtact gatcgcccag atatggagat gatggacgcc ttcaacgcct
      961 tcgaccgaat gtatcgtctt ttccacctta tcgatgtccg ttcccgccgg cacaccgtcc
     1021 agagagagac ggatactgtc gcgtagcagt ccccaggtag aaacaatgat aacgaacgca
     1081 atcaacaggc cgataatcgg gtcgatgata taccatccgg tatgggcaat aacaattccg
     1141 gagacaacca cgccgaccga taccagtgca tcggcagcca tatgcaagta agcacctttc
     1201 acattcaagt ctttatcctt atccttcatg aaaagccatg ccgtaagtgc atttataacg
     1261 actccgatac ctgccaccca agcgatcgca tcaccctcga ccggacgcgg atgcagcaat
     1321 tttcctatac tttcagcaat aataatcccg acggccacca agaggatgac cgcattcagg
     1381 agggatacga gtacagtgct tttcttatat ccataggtat agctgcaagt cgggtgcacc
     1441 ttagccagcc ggaaagccag catagccagt accaggctgg ccacatctcc caggttatgc
     1501 cctgcatccg acagaagtcc taatgaatta taccagaagc cagccgtaaa ttcggctacg
     1561 acaaaagcca ggttcaggga gatacctata atgaaagcct tgttcaaaga ggttatttcg
     1621 tggtggtgat gatcatgatg atgttgttcc atattcattc ctacttttat ttgagtaaca
     1681 atatgggaca aaactatgtt ttttagagca ataaaaatat acctgattat aggtaaatta
     1741 tcagatggtt ataccgtgct tcttaaatat ttctttggca tttgccagac gttcggccga
     1801 taaaggttct acctctttca gtttgtagtc gagaccttga gcctcgtatt tatatgtgcc
     1861 catcgtgtga taaggcaaga gttccgcctt ctgtacaacc gtatagtggc tgatgtattc
     1921 ggccagttta tccaaggctt cgtcattatc cgtcagtccg ggcacaatga catggcgtat
     1981 ccaaaccgga acaccgcgtt tttccagttc gtccaggaaa cgtaacgtat tatccagttt
     2041 cacggccgtt agacggggat gcaggtcggg atcgatggtt tttatatcca ataagaccag
     2101 gtcggcatat tccagaaccg ccagggcttt cggtgtgcag atatagccgg atgtatcgag
     2161 cgccgtatga ataccggctt cccggcaaag acggaaaaac tccctgagaa attcagcttg
     2221 caataaaggt tctcccccgg tgacagtcac gcctcccttg gcgataaagt tcttatatcg
     2281 taaaacctca gccaataatt cttcaggcgt catctgatat ttggcaagac ctttgctatc
     2341 ccaggtatcc ggattgtggc aatacaggca acggagcgga caaccctgca taaagacaac
     2401 aaaacgaatt ccgggaccat caacggtccc gaaactctct agtgaatgta tgtttccttt
     2461 aatttccaac tttcaattac aaagaatgat tgattgttct tgaaatcaca tccagctgct
     2521 gttcgcgtgt cagtttcaca aagtttactg cataacccga tacgcggatt gtcaactgcg
     2581 gatagttttc cggatgctcc atagcatcga gcaacagctc cttgtcgaat acgttcacat
     2641 tcagatgctg tccgccgtca ggagtaaagt aaccgtccag caggccgacc atattggcga
     2701 tctgaatatc acgttctttt cctaacgttg caggagaaac agcgaatgta taagagatac
     2761 cgtcgtgtgc atgctggaac ggcagtttag caacagaagc cagtgcagca acagcgcctt
     2821 tggtatcacg tccgttcatc gggttcgcac ccggagcaaa cggcgtacca ccttcgcgac
     2881 cgtcgggagt tgcacctgtc ttctttccat acaccacatt ggatgtgata gtcaggatag
     2941 actgtgtcgg agtggcgttg cggtatgtct ggtgctgacg cagatattcc atgaactttt
     3001 cagtgatgtc gacagcaata ctatctgtac gatcgtcatt gttaccgaaa ggaacataat
     3061 ctccttcacg ttcgaaaccg acagccaggc cacgttcgtc gcggatcact tttactttcg
     3121 tatcgcggat agcagccaat gagtcggcaa cgatagaaag gccggcaata cctgttgcgc
     3181 ggatacgttc taccgaaccg tcatgcaaag ccatctcgaa tgcttcatac gcatatttat
     3241 cgtgcatgta atggatgatc ttcagtgcat tcacataaac tttagccagc cagcgcatca
     3301 tctgttcgta tttctccatc acttcgttgt aatccaggta ttcgctggtg atcggttcaa
     3361 acctcggagc cacctgtaca cctgatcttt cgtcgcgccc gccgttgatg gcatacagca
     3421 aacatttagc caggttggca cgtgcgccga aatactgcat ctgcttaccg atcttcatcg
     3481 gagacacgca gcaggcaata ccataatcat ctccataatc cggacgcatc agatcgtcgt
     3541 tctcatactg gatagcggaa gtatcgatag aaaccttcgc acagaaacgt ttccagtttt
     3601 caggagcgtt gttgaaccac agtaccgtta agttgggttc cggtgccggg ccgaggttat
     3661 ataatgtatg cagataacgg taagacgtct tcgtcaccaa tgaacggccg tcgacaccct
     3721 gtccgcccaa agactcggtt acccaaacag ggtcaccgga gaacaggtcg ttatattccg
     3781 gagtacgaag gaaacggacg atacgcagtt tgataataaa ctgatcgatc agctcctgag
     3841 cttcttcttc cgtcagcttg ccttcgcgga tatctttttc aatataaata tcgaggaaag
     3901 tagaagtacg tcccaacgac atggcagccc catcctggtc cttcaccgca gccagataac
     3961 ccagatagac aaactgaacc gcttcgcgtg catcctttgc aggcttggtc acgtcgaaac
     4021 cataaccggc acacatcttt tcgaaagcct tcagcgcctt gatctgttcg gatatttcct
     4081 cacggctctg gatgatctct tcggtcagtt cctgaatatc gagtcttttc agataatgtt
     4141 tcttttcagc gatcaggatt tcagtaccgt aaagtgctac acggcggtag tcaccgataa
     4201 tacgtccgcg tccgtaagca tccggcaggc cggtgatgat agcggaacgg cgcgctgcaa
     4261 gcatttcatc tgtataagcg gagaatacgc cttcgttatg cgtctttctg tatttcgtga
     4321 agatttcttt tgtcatcggg tccagtttgt agccatatgc ttccagactg ttttccacca
     4381 tgcgggtacc acatttcggg taaataccgc gtttcagggg ggcatcggtt tgcaggccga
     4441 caattacttc gttttctttg tcaatataac ctggtccgta agtatctata ccttgaggat
     4501 attttgtttc agcgtcgtaa acacccttct ccctttcaac cttaaacatt tcggtcaatt
     4561 tatcccaaac tttcttcgtc ttatctgagg agtctttcaa gaaagagtcg tccccttcgt
     4621 aaggtttgta gttgttctgg ataaaatccc ttacgttaat ctctcttttc caaatttcgc
     4681 ctttgaactg cgcccagttc tcgttttcaa acttcatacc ttattttgtt ttcttcggtt
     4741 ttaatttcgg tgcaaaggta cgttatttat atggcatgcg aaacaatcaa caaaaacatc
     4801 atgtattccc aacggccaaa atccctacaa atagttacta aaacggtaga tatagtgcag
     4861 cgtatcgcct gatataggta tcaaacagga aggattataa ataatatcaa aaaaataaaa
     4921 aaggaaccat ccttcgcagg agagtccctt ttttatgccg gaaacaacca ataaaaaatt
     4981 tccgacacac gtttaatcat cttatatgct tacataatta gcaaaaggtt ttgatatagc
     5041 gattatacta atagtgttac ttttttctat gttgcaaaga tagagccgtc aaggggagta
     5101 cacaatacct attatatagt aaaaaaagag cttcctacta cctattctgt gttataatcg
     5161 ggaaaattga tcattggaat aaacaatatt tgcaaaaatt gctttctttg ataaataaaa
     5221 aagagccatt cttcacagga tggctccctt ttatatatga aagggacaac caataaaaaa
     5281 actcccttcc atgggtattt accttaaata ataggtgtac ataaaaatca acaaatcgga
     5341 ttttggtcag gacgatttat actaatagta aaacttaatt gatacggcaa aggtaaaacc
     5401 acggaaaggc acaggcaata cctatttata tgtaaaacga gctcatttct aaccataaat
     5461 agggattaca gggtaaaata ttacctatat aattattcta ccttcagatt ttcccccgca
     5521 acatatacca cagacctgcc cgcc
//
