LOCUS       c00329_gnlPro..         5419 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|OELEJGKM_329.
ACCESSION   c00329_gnlPro..
VERSION     c00329_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-05 15:25:24
            Original ID  :: gnl|Prokka|OELEJGKM_329
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 5419
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..5419
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[1:4548]"
                     /cutoff="10000"
                     /detection_rule="(minimum(4, [AcetylCoA_hyd_C,
                     AcetylCoA_hydro, B12-binding, Biotin_lipoyl, Glyoxalase_4,
                     HMGL-like, MM_CoA_mutase, MeaB, OAD_beta, OAD_gamma,
                     PYC_OADA]) and minimum(1, [CT_Btheta, CT_Propionyl_CoA1,
                     CT_Propionyl_CoA2, CT_Propionyl_CoA3, CT_Vparvula]) and not
                     Cytidylate_kin)"
                     /neighbourhood="10000"
                     /product="succinate2propionate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      2..4548
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="10000"
                     /detection_rule="(minimum(4, [AcetylCoA_hyd_C,
                     AcetylCoA_hydro, B12-binding, Biotin_lipoyl, Glyoxalase_4,
                     HMGL-like, MM_CoA_mutase, MeaB, OAD_beta, OAD_gamma,
                     PYC_OADA]) and minimum(1, [CT_Btheta, CT_Propionyl_CoA1,
                     CT_Propionyl_CoA2, CT_Propionyl_CoA3, CT_Vparvula]) and not
                     Cytidylate_kin)"
                     /neighbourhood="10000"
                     /product="succinate2propionate"
                     /protocluster_number="1"
     cand_cluster    1..5419
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(minimum(4, [AcetylCoA_hyd_C,
                     AcetylCoA_hydro, B12-binding, Biotin_lipoyl, Glyoxalase_4,
                     HMGL-like, MM_CoA_mutase, MeaB, OAD_beta, OAD_gamma,
                     PYC_OADA]) and minimum(1, [CT_Btheta, CT_Propionyl_CoA1,
                     CT_Propionyl_CoA2, CT_Propionyl_CoA3, CT_Vparvula]) and not
                     Cytidylate_kin)"
                     /kind="single"
                     /product="succinate2propionate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..5419
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="succinate2propionate"
                     /region_number="1"
                     /rules="(minimum(4, [AcetylCoA_hyd_C, AcetylCoA_hydro,
                     B12-binding, Biotin_lipoyl, Glyoxalase_4, HMGL-like,
                     MM_CoA_mutase, MeaB, OAD_beta, OAD_gamma, PYC_OADA]) and
                     minimum(1, [CT_Btheta, CT_Propionyl_CoA1,
                     CT_Propionyl_CoA2, CT_Propionyl_CoA3, CT_Vparvula]) and not
                     Cytidylate_kin)"
                     /tool="gutsmash"
     CDS             2..352
                     /gene_functions="biosynthetic (rule-based-clusters)
                     succinate2propionate: Glyoxalase_4"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg329_1"
                     /sec_met_domain="Glyoxalase_4 (E-value: 9.9e-21, bitscore:
                     62.9, seeds: 51, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="YENVLGLKCYNIETVEDQKVRTAFLKVGDVKIELLEPTSPESTIA
                     KFIEKNNGNGGMHHLAFAVEDGVAGALAEVEEKGIRLIDKAPRKGAEGLNIAFLHPKST
                     LGVLTELCEKPE"
     CDS             411..1964
                     /gene_functions="biosynthetic (rule-based-clusters)
                     succinate2propionate: CT_Btheta"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg329_2"
                     /sec_met_domain="CT_Btheta (E-value: 6.2e-292, bitscore:
                     956.9, seeds: 72, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSNQLEKIKELIDRRAAARLGGGEKAIAKQHEKGKYTARERIAML
                     LDEGSFEEMDMFVEHRCTNFGMEKKHYPGDGVVTGCGTIEGRLVYIFAQDFTVSAGSLS
                     ETMSLKICKIMDQAMKMGAPCIGINDSGGARIQEGINALAGYAEIFQRNILASGVIPQI
                     SGIFGPCAGGAVYSPALTDFTLMMEGTSYMFLTGPKVVKTVTGEDVSQENLGGASVHST
                     KSGVTHFTAQTEEEGLALIRKLLSYIPQNNLEEAPYVDCTDPIDRLEDSLNDIIPDNPN
                     KPYDMYEVIGAIIDNGEFLEIQKDYSKNIIIGFARFNGQSVGIVANQPKYLAGVLDSNA
                     SRKGARFVRFCDAFNIPIVSLVDVPGFLPGTGQEYNGVILHGAKLLYAYGEATVPKVTI
                     TLRKSYGGSHIVMSCKQLRGDMNYAWPTAEIAVMGGAGAVEVLYAREAKEQENPAQFLA
                     EKEAEYTKLFANPYNAAKYGYIDDVIEPRNTRFRIIRALQQLQTKKLTNPAKKHGNIPL
                     "
     CDS             1996..2928
                     /gene_functions="biosynthetic (rule-based-clusters)
                     succinate2propionate: OAD_gamma"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg329_3"
                     /sec_met_domain="OAD_gamma (E-value: 5.1e-18, bitscore:
                     54.3, seeds: 234, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNKTKIGIFLSLMLVLGFCSSCKEQKSNNKLLLNEVLVNNESNYQ
                     DDYGVHSAWIEIFNRSYGSADLAGCYLKFSSQPGDTASYFIPKGDVLTLIKPRQHALFW
                     ADGEPRRGTFHTNFKLDATNANWIGLYDSGKKLLDQVIVPAGTLKANQSYARVSDAADQ
                     WEVKGGSEDKYVTPSTNNKTIDSNAKMEKFEEHDSVGIGMAISAMSVVFCGLILLYISF
                     KIIGKISVNLSKRNAMKAKGITDKQEAKEKQLGQAPGEVFAAISMAMHEFQSDVHDVED
                     TILTITRVKRSYSPWSSKIYTLRETPHKK"
     CDS             2952..3386
                     /gene_functions="biosynthetic (rule-based-clusters)
                     succinate2propionate: Biotin_lipoyl"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1024:Biotin-requiring enzyme (Score: 67.3; E-value:
                     2.7e-21)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg329_4"
                     /sec_met_domain="Biotin_lipoyl (E-value: 2.6e-22, bitscore:
                     67.3, seeds: 40, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKEYKYKINGNVYKVAIGDIEDNIAHVEVNGTPYKVEMEKAPKVV
                     VKPVARPAATSAPAAPATPVVKPAAASTGKSGVKSPLPGVILDIKVNVGDTVKKGQVII
                     ILEAMKMENNINADKDGKITAINVNKGDSVLEGTDLVIIE"
     CDS             3388..4548
                     /gene_functions="biosynthetic (rule-based-clusters)
                     succinate2propionate: OAD_beta"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg329_5"
                     /sec_met_domain="OAD_beta (E-value: 2.3e-153, bitscore:
                     498.9, seeds: 229, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MGEFITFLGNNLADFWTYTGFANATVGHVVMLLVGLFFIYLAVAK
                     EFEPMLLIPIGFGMLIGNIPFNMEAGLKVGIYEEGSVLNILYQGVTSGWYPPLIFLGIG
                     AMTDFSALISNPKLMLVGAAAQFGIFGAYMIALAIGFDPMQAGAIGIIGGADGPTAIFL
                     SSKLAPNLMGAIAVSAYSYMALVPVIQPPIMRWLTSKNERLIRMKPPRVVSHTEKVMFP
                     IIGLLLTTFLVPSGLPLLGMLFFGNLLKESGVTRRLANTASGPLIDTITILLGLTVGAS
                     TQASEFLTLDSIKIFGLGALSFVIATASGVIFVKIFNLFLKKGNKINPLIGNAGVSAVP
                     DSARISQVVGLEYDPTNYLLMHAMGPNVAGVIGSAVAAGILLGFLM"
     CDS             4718..5419
                     /locus_tag="ctg329_6"
                     /transl_table=11
                     /translation="MRKLFISLCFSACCSLLAAQTTNPIQEAMANYDYETALMLIDQET
                     PTVPLLYQKGKALKGLGNNLEALSVFQEVVAQDSLNPRAYIEAAECCKSLAKYSEALDY
                     YQNALHINPDNKYARIQYISLLMNMKRYRESLKESNLLAERDSSTYVLHLRAESMGQVY
                     DNTEIMHVIDAYLDIQKRYPNDYLSAAKLGNIYVAGHQYEDAINITEKYRSIDSTNVLV
                     NRINAQAYCLNK"
ORIGIN
        1 ctacgaaaac gttttaggtt tgaaatgcta caacatcgaa accgtggaag atcagaaagt
       61 tagaaccgct ttcctgaaag tgggcgatgt aaagatcgaa ttgttggaac ctacttctcc
      121 cgaaagcaca attgccaagt tcatcgaaaa gaacaacggt aacggtggca tgcatcatct
      181 cgcattcgcc gttgaagacg gagtggccgg tgcgcttgcc gaagtcgaag aaaaaggtat
      241 ccgccttata gacaaagctc cccgcaaagg tgcagaaggt ctgaacattg cattcctgca
      301 tccgaagtca actctcggtg tcctgaccga gctttgcgag aaaccggaat aagcaagact
      361 gtaaaatcat caaatcataa attgtataaa cacttaaaga atataaattc atgagtaatc
      421 aacttgaaaa gattaaagag cttatagacc gccgcgctgc agcacgtctc ggtggtggtg
      481 aaaaagcgat tgcaaagcaa cacgagaagg gtaaatatac agctcgcgag cgcatagcca
      541 tgcttctcga cgaaggcagt ttcgaagaaa tggacatgtt cgtagaacat agatgcacca
      601 acttcggcat ggaaaagaaa cactaccccg gtgacggtgt agtaaccggt tgcggtacaa
      661 tcgaaggccg tctggtttat atcttcgcac aggacttcac agtttctgcc ggttcactgt
      721 ccgaaaccat gtctctgaaa atctgtaaga ttatggacca agccatgaaa atgggtgctc
      781 cgtgtatcgg tatcaacgac tcgggcggtg cacgtattca ggaaggtatt aatgccttgg
      841 ctggctacgc tgaaatcttc caacgtaaca tacttgcttc gggtgtcatt ccgcagattt
      901 ccggtatttt cggtccttgt gccggtggcg ccgtttactc tcctgccttg acagacttta
      961 ctttgatgat ggaaggtaca tcctacatgt tccttaccgg tccgaaggta gtaaagactg
     1021 taaccggaga agacgtaagc caggaaaacc tgggtggcgc gagcgttcac tccaccaaat
     1081 ctggtgttac tcattttact gcacagacag aagaagaagg tttggccctt atccgcaaac
     1141 tgttgagcta cattccccag aacaatctgg aagaagctcc ctatgtagac tgtacagacc
     1201 ctattgaccg tctggaagat tccctgaacg atatcattcc tgataacccg aacaaaccgt
     1261 acgatatgta cgaggtaatt ggcgctatca tagataatgg tgagttcctc gaaatccaaa
     1321 aagattattc aaagaatatc attattggtt tcgcacgttt caacggacag tccgtaggta
     1381 ttgttgccaa ccagccgaag tatctggcag gtgtgctcga cagcaacgca tcccgcaagg
     1441 gcgcacgctt cgtccgtttc tgcgacgcct tcaatattcc tatcgtatca ttggtagacg
     1501 taccgggatt ccttccggga accggccagg agtacaacgg cgtcatcctg cacggtgcca
     1561 aactgctgta tgcatacggt gaagctactg tacccaaggt taccatcaca ctgcgtaaat
     1621 cctacggtgg ttcgcacatc gtgatgagct gtaagcagct gcgtggcgac atgaattatg
     1681 cttggcctac tgctgaaatc gctgtaatgg gtggtgcagg tgctgtagaa gtattatatg
     1741 cccgcgaagc caaagagcag gaaaatccgg ctcaattcct ggctgaaaaa gaggctgaat
     1801 acaccaagtt gtttgccaat ccttataatg cagctaaata tggctacatt gatgatgtta
     1861 tcgaaccgcg taacacacgt ttccgcatca ttcgtgccct gcaacagttg caaaccaaga
     1921 aattgactaa cccggctaag aagcatggta atattccctt gtaatcaagc ttttcacact
     1981 aaaacaagaa cgattatgaa taaaacaaaa atcggaatat tcctttcatt gatgctggtg
     2041 ttaggctttt gctcttcttg caaagaacag aaatcgaaca acaaactgct gctgaatgaa
     2101 gtgctggtca acaacgaaag caactatcag gatgactacg gtgtgcacag cgcatggatt
     2161 gaaatattca acagatcata cggcagcgcc gaccttgcag gttgctatct gaaattcagc
     2221 agccagccgg gtgatactgc cagttatttc attcccaaag gtgacgttct gacactcata
     2281 aaaccacgtc agcatgcact tttctgggct gatggagaac caagacgcgg tacatttcat
     2341 acgaacttca aactggatgc cacaaatgcc aactggattg ggttgtatga ttccggcaag
     2401 aaattgcttg atcaagtcat agtgcctgcc ggtactttaa aagccaacca atcctatgcc
     2461 cgtgtcagtg atgcagccga ccaatgggaa gtaaagggtg gaagcgaaga taaatatgtc
     2521 actccgagta ccaacaacaa gactatcgac agcaatgcca aaatggaaaa attcgaagag
     2581 catgacagcg taggtatcgg tatggctatt tctgccatga gcgttgtatt ctgcgggctg
     2641 atattactct acatctcctt caagattata ggtaaaatat cagttaacct cagcaaacgc
     2701 aatgccatga aagctaaagg tattactgat aaacaagaag caaaagagaa acaactgggg
     2761 caggctccgg gagaagtttt tgctgccatt tcaatggcca tgcacgaatt ccaaagcgat
     2821 gtacacgatg tggaagacac gatacttacc attacccgtg taaaacgcag ttactcaccg
     2881 tggagttcaa agatttatac gttgcgtgag actcctcaca aaaagtaagt cacccataat
     2941 aaaataaaac gatgaaagaa tataaatata aaattaacgg taacgtatat aaagttgcca
     3001 ttggagatat tgaagacaac atcgctcacg tagaagtgaa cggtactccc tacaaagttg
     3061 aaatggaaaa agcaccgaag gtagttgtta aaccggtagc acgtccggca gccacatccg
     3121 ctcctgctgc tcctgctact ccggtagtaa aaccggccgc tgcttctacc ggcaagtcag
     3181 gtgtaaaatc tccgcttccc ggtgtaatcc tcgatatcaa agtaaatgtc ggcgatacag
     3241 taaagaaggg acaagttatc atcattctgg aagccatgaa gatggaaaac aatatcaatg
     3301 ctgataaaga cggtaagatt acagccatca atgtaaacaa aggggactct gttctcgaag
     3361 gtactgacct cgtaattatt gaataatatg ggagaattta tcacattttt aggaaacaac
     3421 cttgccgact tctggactta tacaggtttt gccaatgcaa cggtaggaca tgtggtaatg
     3481 cttctggtag gtttgttttt catatacttg gctgtagcca aggaatttga gccgatgcta
     3541 ttaattccta tcggtttcgg tatgctgatt ggtaatatac ctttcaatat ggaagccgga
     3601 ttgaaagtcg gtatctatga agaaggttcc gtacttaata ttctgtacca gggagtaacc
     3661 tccggttggt acccgccact catattcctg ggtatcggtg ccatgacgga tttctccgct
     3721 ttgatatcga accccaaatt aatgttggtt ggtgcagcag cccaattcgg tatattcggt
     3781 gcttatatga ttgcccttgc cataggattt gacccgatgc aggctggtgc aatcggtatc
     3841 attggtggtg ccgatggtcc gactgccatc ttcctctctt ccaaactggc tcctaactta
     3901 atgggtgcca ttgcggtatc agcctattca tatatggcat tagtaccggt gatacagccg
     3961 cctattatgc gttggctgac cagtaaaaat gaacgtctta tccgtatgaa acctccccgt
     4021 gtggtttcac atacggaaaa agtaatgttc ccgattatcg gtttgttact aaccacattc
     4081 cttgtaccat ccggtctgcc tttgctgggt atgctgttct tcggtaattt actgaaagaa
     4141 agtggagtta cacgtcgtct ggcaaacaca gccagtggtc cgctgattga taccattact
     4201 attctgcttg gtttgacagt aggtgcttct acgcaagcat ccgagttctt aacccttgat
     4261 tcaatcaaga tattcggtct gggcgcctta tcatttgtca ttgccacagc atccggtgtc
     4321 atcttcgtga agattttcaa cctcttcctg aagaaaggca acaagattaa tccgttgatc
     4381 ggtaatgccg gtgtatctgc cgtacccgac tctgcacgta tctcccaagt agtaggtctg
     4441 gaatacgatc ctaccaacta tctgctgatg catgctatgg gcccgaacgt tgccggtgta
     4501 atcggttccg ctgtagcagc aggtatcctg ctgggattct taatgtaata catttattca
     4561 ttccgttatc cggcttgaat tctgaactat tttagagccc cttcattttt ggaggggctt
     4621 tttcgttaat ctatctaaaa cagattacga ttatttcgta aatacattct ttttagtaac
     4681 ttgcagtcag tttaacctca tataaatgat atactgtatg cgaaagcttt ttatctcctt
     4741 atgtttcagt gcatgctgtt cgcttctggc ggcacagacc acaaatccca ttcaagaggc
     4801 gatggccaac tacgattatg aaacagcctt gatgctgatc gatcaagaaa ccccgaccgt
     4861 ccccctactc taccaaaaag ggaaagcgct gaaagggctg ggcaataacc ttgaagctct
     4921 gagcgtattt caggaagtcg tagctcagga ttctctcaat ccccgggcat acattgaagc
     4981 tgccgaatgc tgcaaatcac ttgccaaata tagcgaggca ctggattact accagaatgc
     5041 gctccacata aatccggaca ataaatacgc acgcattcag tatatcagtc tgctgatgaa
     5101 catgaagaga taccgtgagt ctctaaagga aagtaacctg ctggcagaaa gggacagttc
     5161 cacctatgta ctacacctta gagccgaaag tatggggcaa gtctatgaca acacagaaat
     5221 catgcatgtg atagatgcct acctggacat acagaaaaga tatcccaatg attacctgtc
     5281 tgctgccaaa ctcggcaaca tctatgtagc aggccaccag tatgaagacg ccataaacat
     5341 aacggagaaa taccgttcca tagactctac caacgtcctt gtgaaccgca tcaatgccca
     5401 agcctattgc ctgaacaaa
//
