LOCUS       c00340_gnlPro..         7722 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|DODPMHAE_340.
ACCESSION   c00340_gnlPro..
VERSION     c00340_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-07 19:17:20
            Original ID  :: gnl|Prokka|DODPMHAE_340
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 7722
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..7722
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[1565:4545]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      1566..4545
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..7722
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..7722
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             162..791
                     /locus_tag="ctg340_1"
                     /transl_table=11
                     /translation="MITQYDLYETPSPKGEDGKKSLHARVCPKKTYTSQEFVEHVAAFE
                     HLPKNQIGGALAAIVDDLCYLLADGNIVELGDLGFFSTSLKCLKDTDDDEDEIKPKSIA
                     FQNVHLRISSEFRKRIKHEMKFQRVHSLTRRPKIVNSTVKERMKKMVSFLKINICITRK
                     EYIGLVGLTPYEAMNELNDFISQGLLRRRGAGRSVVYVAGENLKVE"
     CDS             1566..3806
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate2"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg340_2"
                     /sec_met_domain="Gly_radical (E-value: 1.6e-45, bitscore:
                     142.8, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate2 (E-value: 5e-285, bitscore:
                     935.1, seeds: 33, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKFENENWAQFKGEIWKREINVRDFIQNNYKPYEGDDSFLKDSSD
                     KTKKVWDKLTEMFKVEREKGVYDAETKYPQGIDTYGPGYIDKENEVIVGLQTDAPLKRG
                     IYPKCGTRMVENSLEAYGYKLDPMTKEIFTKYRKTHNEGVFSAYTDEMLAARRSAIITG
                     LPDAYGRGRIIGDYRRVALYGTEILIAEKKHYLKRLDIQELTEEIIQSREEISEQIKAL
                     KAFEKMCAGYGFDVTKPAKDAREAVQFVYLGYLAAVKDQDGAAMSLGRTSTFLDIYIEK
                     DIREGKLTEEEAQELIDQFIIKLRIVRFLRTPEYNDLFSGDPVWVTESLGGQGVDGRSL
                     VTKTSYRYLHTLYNLGPAPEPNLTVLWFNNAPENWKRFCAKVSIDTSAIQYENDDLMRP
                     DYGDDYGIACCVSPMKIGKQMQYFGARANLAKCLLYAINGGRDERSGVQVAPRFEPITS
                     EYLDYNEVMEKYEQMMRWLAKVYVNALKIIHYMHDKYAYEAFEMALHDGSVERIRATGI
                     AGLSIVADSLAAIRDTKVKVIRDERGLAVGFEREGDYVPFGNNDDRTDSIAVDITEKFM
                     EYLRQHQTYRNATPTQSILTITSNVVYGKKTGATPDGREGGTPFAPGANPMNGRDTKGA
                     VAALASVAKLPFQHAHDGISYTFAVSPATLGKERDIQIANMVGLLDGYFTPDGGQHLNV
                     NVFDKELLLDAMEHPENYPQLTIRVSGYAVNFVKLTREQQLDVISRTINHSL"
     CDS             3814..4545
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 72.3;
                     E-value: 1.6e-22)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg340_3"
                     /sec_met_domain="Radical_SAM (E-value: 5.5e-37, bitscore:
                     116.4, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="LEIKGNIHSLESFGTVDGPGIRFVVFMQGCPLRCLYCHNPDTWDS
                     KGLAKYQMTPEELLAEVLRYKNFIAKGGVTVTGGEPLLQAEFLREFFRLCREAGIHTAL
                     DTSGYICTPKALAVLEYADLVLLDIKTIDPDLHPRLTAVKLDNTLRFLDELEKRGVPVW
                     IRHVIVPGLTDNDEALDKLAEYISHYTVVQKAELLPYHTMGAYKYEAQGLDYKLKEVEP
                     LSAERLANAKEIFKKHGITIW"
     CDS             4631..5533
                     /locus_tag="ctg340_4"
                     /transl_table=11
                     /translation="MEQHHHDHHHHEITSLNKAFIIGISLNLAFVVAEFTAGFWYNSLG
                     LLSDAGHNLGDVASLVLAMLAFRLAKVHPTCSYTYGYKKSTVLVSLLNAVILLVAVGII
                     IAESIGKLLHPRPVEGDAIAWVAGIGVVINALTAWLFMKDKDKDLNVKGAYLHMAADAL
                     VSVGVVVSGIVIAHTGWYIIDPIIGLLIAIVIIVSTWGLLRDSIRLSLDGVPAGTDIDK
                     VEKTIHSVEGVEGVHHLHIWAISTTETALTAHVSVADISEMERIKQEIKHDLQHVGIQH
                     ATLEFETSGFKCDGHCCSD"
     CDS             5694..6260
                     /locus_tag="ctg340_5"
                     /transl_table=11
                     /translation="MEPIINSQAPEFKVQAYHNGSFKTVSSDDIKGKWAIFFFYPADFT
                     FVCPTELVDMADKYDQFQAMGVEVYSVSTDSHFVHKAWHDASETIRKIKYPMLADPTGV
                     LSRAFGVMIEEDGMAYRGTFLVNPEGKIKLAEINDNGIGRNADELLRKVEAAQFIAEHP
                     SEVCPAKWKKGGETLKPSIDLVGKI"
     CDS             6354..7721
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Pyr_redox_2"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg340_6"
                     /sec_met_domain="Pyr_redox_2 (E-value: 1.4e-35, bitscore:
                     111.6, seeds: 65, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MLDSSMRNQLSGIFSGLTNEYIFDIQVSPEHESRKELLELLNDVA
                     SCSDKITVQVADGEGLEFSLLKNGEKTGIKFRGVPNGHEFTSLLLAILNSDGKGKNIPD
                     ESICNRVKALNGPVKLTTYVSLTCTNCPDVVQALNAMATLNPEISHEMVDGAINQEEVA
                     AMKVQGVPSVFADGKLIHVGRGDFGELLGKLEAQYGVKEENLSVVEKNYDVIIVGGGPA
                     GSAAAIYTARKGLAVAVVADRIGGQVKETVGIENMISVSQTTGQQLALDLMSHMKDYPI
                     DILEHRRIEKVEVEGREKLLTTSTGEKLIAPAVIVATGASWRRLNVPGESEYIGRGVAF
                     CPHCDGPFYKGKHVAVVGGGNSGVEAAIDLAGICSKVTLLEFADELKADQVLQDKARSL
                     PNVEIFMHSQTMEVLGNGEKVTGIRIKDRKTEEVRNIELDGIFVQIGLAANSGPFRE"
ORIGIN
        1 atgcatttaa cagctatcga ataatcattc gacaattgtc gaacgaataa agaatgattg
       61 tcgaatgatt ttttttctag aagcgaaatt tatttttaga agtatttcga tttatcaggg
      121 agaaacaagt tatatttgaa caattaatat caatataacc tatgatcaca caatacgacc
      181 tgtatgaaac accttcaccc aaaggggaag acggtaaaaa atcgctgcat gcccgtgttt
      241 gtccgaaaaa gacttacacc agtcaagagt ttgtagaaca tgtggcagcc tttgaacatc
      301 tgccgaagaa tcagattggc ggggcacttg ctgccattgt cgacgatctt tgttatctgc
      361 ttgccgatgg aaacattgtg gaattaggtg atcttggatt tttttctact tcattgaagt
      421 gcctgaagga tacggatgac gatgaggatg aaatcaaacc gaaatcgatc gcttttcaaa
      481 atgtacatct gagaatcagc agtgaattcc gaaaaagaat caaacatgag atgaagtttc
      541 aacgcgtgca ttcccttacg cggagaccaa aaattgtcaa ttctaccgtg aaagagcgga
      601 tgaaaaaaat ggtctctttc ctcaaaataa acatttgtat tacccgaaag gaatatatag
      661 gactggtcgg attgacacct tacgaggcga tgaatgaact gaacgatttt atcagccagg
      721 ggcttcttcg ccgtcgcggg gcgggcaggt ctgtggtata tgttgcgggg gaaaatctga
      781 aggtagaata attatatagg taatattttc cctgtaatcc ctatttatgg ttagaaatga
      841 gctcgtttta catatatata ggtattgcct gtgcctttcc gtggttttac ctttgccgta
      901 tcaattaagt tttactatta gtataaatcg tcctgaccaa aatccgattt gttgattttt
      961 atgtacacct attatttaag gtaaataccc atggaaggga gtttttttat tggttgtccc
     1021 tttcatatat aaaagggagc catcctgtga agaatggctc ttttttattt atcaaagaaa
     1081 gcaatttttg caaatattgt ttattccaat gatcaatttt cccgattata acacagaata
     1141 ggtagtagga agctcttttt ttactatata ataggtattg tgtactcccc ttgacggctc
     1201 tatctttgca acatagaaaa aagtaacact attagtataa tcgctatatc aaaacctttt
     1261 gctaattatg taagcatata agatgattaa acgtgtgtcg gaaatttttt attggttgtt
     1321 tccggcataa aaaagggact ctcctgcgaa ggatggttcc ttttttattt ttttgatatt
     1381 atttataatc cttcctgttt gatacctata tcaggcgata cgctgcacta tatctaccgt
     1441 tttagtaact atttgtaggg attttggccg ttgggaatac atgatgtttt tgttgattgt
     1501 ttcgcatgcc atataaataa cgtacctttg caccgaaatt aaaaccgaag aaaacaaaat
     1561 aaggtatgaa gtttgaaaac gagaactggg cgcagttcaa aggcgaaatt tggaaaagag
     1621 agattaacgt aagggatttt atccagaaca actacaaacc ttacgaaggg gacgactctt
     1681 tcttgaaaga ctcctcagat aagacgaaga aagtttggga taaattgacc gaaatgttta
     1741 aggttgaaag ggagaagggt gtttacgacg ctgaaacaaa atatcctcaa ggtatagata
     1801 cttacggacc aggttatatt gacaaagaaa acgaagtaat tgtcggcctg caaaccgatg
     1861 cccccctgaa acgcggtatt tacccgaaat gtggtacccg catggtggaa aacagtctgg
     1921 aagcatatgg ctacaaactg gacccgatga caaaagaaat cttcacgaaa tacagaaaga
     1981 cgcataacga aggcgtattc tccgcttata cagatgaaat gcttgcagcg cgccgttccg
     2041 ctatcatcac cggcctgccg gatgcttacg gacgcggacg tattatcggt gactaccgcc
     2101 gtgtagcact ttacggtacg gaaatcctga tcgctgaaaa gaaacattat ctgaaaagac
     2161 tcgatattca ggaactgacc gaagagatca tccagagccg tgaggaaata tccgaacaga
     2221 tcaaggcgct gaaggctttc gaaaagatgt gtgccggtta tggtttcgac gtgaccaagc
     2281 ctgcaaagga tgcacgcgaa gcggttcagt ttgtctatct gggttatctg gctgcggtga
     2341 aggaccagga tggggctgcc atgtcgttgg gacgtacttc tactttcctc gatatttata
     2401 ttgaaaaaga tatccgcgaa ggcaagctga cggaagaaga agctcaggag ctgatcgatc
     2461 agtttattat caaactgcgt atcgtccgtt tccttcgtac tccggaatat aacgacctgt
     2521 tctccggtga ccctgtttgg gtaaccgagt ctttgggcgg acagggtgtc gacggccgtt
     2581 cattggtgac gaagacatct taccgttatc tgcatacatt atataacctc ggcccggcac
     2641 cggaacccaa cttaacggta ctgtggttca acaacgctcc tgaaaactgg aaacgtttct
     2701 gtgcgaaggt ttctatcgat acttccgcta tccagtatga gaacgacgat ctgatgcgtc
     2761 cggattatgg agatgattat ggtattgcct gctgcgtgtc tccgatgaag atcggtaagc
     2821 agatgcagta tttcggcgca cgtgccaacc tggctaaatg tttgctgtat gccatcaacg
     2881 gcgggcgcga tgaaagatca ggtgtacagg tggctccgag gtttgaaccg atcaccagcg
     2941 aatacctgga ttacaacgaa gtgatggaga aatacgaaca gatgatgcgc tggctggcta
     3001 aagtttatgt gaacgcactg aagatcatcc attacatgca cgataaatat gcgtatgaag
     3061 cattcgagat ggctttgcat gacggttcgg tagaacgtat ccgcgcaaca ggtattgccg
     3121 gcctttctat cgttgccgac tcattggctg ctatccgcga tacgaaagta aaagtgatcc
     3181 gcgacgaacg tggcctggct gtcggtttcg aacgtgaagg cgattatgtt cctttcggta
     3241 acaatgacga tcgtacagat agtattgctg tcgacatcac tgaaaagttc atggaatatc
     3301 tgcgtcagca ccagacatac cgtaatgcaa ctccgacaca gtctatcctg actattacgt
     3361 ctaatgtcgt atacggaaag aagacaggtg caactcccga cggtcgcgaa ggtggtacgc
     3421 cgtttgctcc gggtgcgaac ccgatgaacg gacgtgatac caaaggcgct gttgctgcac
     3481 tggcttctgt tgctaaactg ccgttccagc atgcacacga cggtatctct tatacattcg
     3541 ctgtttctcc tgcaacgtta ggaaaagaac gtgatattca gatcgccaat atggtcggcc
     3601 tgctggacgg ttactttact cctgacggcg gacagcatct gaatgtgaac gtattcgaca
     3661 aggagctgtt gctcgatgct atggagcatc cggaaaacta tccgcagttg acaatccgtg
     3721 tatcgggtta tgcagtaaac tttgtgaaac tgacacgcga acagcagctg gatgtgattt
     3781 caagaacaat caatcattct ttgtaattga aagttggaaa ttaaaggaaa catacattca
     3841 ctagagagtt tcgggaccgt tgatggtccc ggaattcgtt ttgttgtctt tatgcagggg
     3901 tgtccgctcc gttgcctgta ttgccacaat ccggatacct gggatagcaa agggcttgcc
     3961 aaatatcaga tgacgcctga agaattattg gctgaggttt tacgatataa gaactttatc
     4021 gccaagggtg gcgtgactgt caccggggga gaacccttat tgcaagctga atttctcagg
     4081 gagtttttcc gtctttgccg ggaagccggt attcatacgg cgctcgatac atccggctat
     4141 atctgcacac cgaaagccct ggcggttctg gaatatgccg acctggtctt attggatata
     4201 aaaaccatcg atcccgacct gcatccccgt ctaacggccg tgaaactgga taatacgtta
     4261 cgtttcctgg acgaactgga aaaacgcggt gtcccggttt ggatacgcca tgtcattgtg
     4321 cccggactga cggataatga cgaagccttg gataaactgg ccgaatacat cagccactat
     4381 acggttgtac agaaggcgga actcttgcct tatcacacga tgggcgctta taaatacgag
     4441 gcacaaggtc tcgactacaa actgaaagag gtagaacctt tatcggccga acgtctggca
     4501 aatgccaaag aaatatttaa gaagcacggt ataaccatct ggtaatttac ctataatcag
     4561 gtatattttt attgctctaa aaaacatagt tttgtcccat attgttactc aaataaaagt
     4621 aggaatgaat atggaacaac atcatcacga tcatcaccac cacgaaataa cctctttgaa
     4681 caaggctttc attataggta tttccctgaa cctggctttt gtcgtagccg aatttacggc
     4741 tggcttctgg tataattcat taggactttt gtcggacgca gggcataacc tgggagatgt
     4801 agccagcctg gtactggcta tgctggcttt ccggctggct aaggtgcacc cgacttgcag
     4861 ctatacctat ggatataaga aaagcactgt actcgtatcc cttctgaatg cggtcatcct
     4921 cttggtggcc gtcgggatta ttattgctga aagtatagga aaactgctgc atccgcgtcc
     4981 ggtcgagggt gatgcgatcg cttgggtggc aggtatcgga gtcgttataa atgcacttac
     5041 ggcatggctt tttatgaagg ataaggataa agacttgaat gtgaaaggtg cttacttaca
     5101 tatggctgcc gatgcactgg tatcggtcgg cgtggttgtc tccggaattg ttattgccca
     5161 taccggatgg tatatcatcg acccgattat cggcctgttg attgcgatcg ttatcattgt
     5221 ttctacctgg ggactgctgc gcgacagtat ccgtctctct ctggacggtg tgccggcggg
     5281 aacggacatc gataaggtgg aaaagacgat acattcggtc gaaggcgttg aaggcgtcca
     5341 tcatcttcat atctgggcga tcagtacgac cgaaacggca cttaccgccc atgtttccgt
     5401 tgcggatatt tcagaaatgg aacgtatcaa acaggagata aaacacgacc ttcagcatgt
     5461 gggaatccag catgctacgc tcgaatttga aacgtcggga ttcaagtgcg atgggcactg
     5521 ttgctcagac tgaaacaggt atattataaa cctgatttac tgatactcaa agattttgtc
     5581 tatgactgca taaaaccttt cgattggatt ttttgttctt acctcgaatc ggcaacttat
     5641 ctttgcacca gtaacaaaaa caaaatagta ataacgatta aaataaaaat tctatggaac
     5701 ctattatcaa ttcacaagca cccgagttta aagttcaggc ataccacaac ggtagtttca
     5761 aaactgtaag cagcgacgac atcaaaggca aatgggcgat tttctttttc tacccggctg
     5821 actttacatt tgtttgtccg accgagttgg tagatatggc tgacaaatat gaccagttcc
     5881 aggcaatggg tgtagaagtt tattctgtaa gtacagattc acacttcgta cacaaagcat
     5941 ggcacgatgc atctgaaacg attcgtaaga tcaaataccc gatgttggca gacccgacag
     6001 gcgtattgag ccgtgcattc ggtgtaatga tcgaagaaga cggtatggct taccgcggta
     6061 cattcctggt aaatccggaa ggaaagatca aactggcaga aatcaacgat aacggtatcg
     6121 gccgtaacgc cgacgaactg cttcgtaaag tagaagctgc ccagttcatc gccgaacatc
     6181 cgagcgaagt ttgccccgcc aaatggaaaa aaggtggcga aacattgaaa ccgagcatcg
     6241 atctggtcgg taagatctaa tagtaaaact ggaggccggc aacatattgt cggccttttt
     6301 tatctaaaca aatatcagtt gttctattca taattaaaat aaaggattca actatgttag
     6361 attcatcaat gagaaatcag ttgtccggta tctttagcgg attgacgaac gaatatatat
     6421 ttgatataca agtttctcct gaacatgaaa gcaggaagga actattggaa ttgctgaacg
     6481 atgtggcttc ctgttccgat aagatcaccg tacaggtggc agacggcgaa ggcctggagt
     6541 tctctctgct gaaaaacggg gaaaaaaccg gtattaaatt ccggggcgtt cccaacggac
     6601 acgaatttac ttcgttgctg ctggctatcc tgaacagtga cggcaaaggc aaaaatattc
     6661 ccgacgaaag tatttgtaac cgtgtgaagg ccctgaatgg cccggtcaaa ctgactacat
     6721 atgtttcttt gacctgtacc aactgtcctg acgttgtaca ggctttgaat gccatggcta
     6781 cactgaaccc ggaaatctct cacgaaatgg tggacggtgc gatcaaccag gaagaggttg
     6841 ctgccatgaa agtgcaggga gtaccttccg tatttgccga cggtaaactg atccatgtag
     6901 gtcgtggaga ctttggcgaa ctgcttggca aactcgaagc gcagtatggc gtgaaagagg
     6961 agaacctgtc ggtggtagaa aagaactacg atgttatcat cgtcggcgga ggtcctgccg
     7021 gaagtgcggc agctatctat actgcccgta aaggtttggc ggtggctgtt gtggccgacc
     7081 gtatcggcgg acaggtgaag gaaactgtcg gtatcgagaa tatgatttct gtttcgcaga
     7141 cgacggggca gcagctggct ctcgacctga tgtcgcatat gaaagattat ccgatcgata
     7201 tccttgaaca ccgccgtatc gagaaggtgg aagtcgaagg acgcgaaaag ctgctgacaa
     7261 cctctaccgg cgaaaaactg atagccccgg ccgttatcgt agcaaccgga gctagctggc
     7321 gcaggttgaa tgtccccgga gaatccgaat atatcggacg cggtgtcgct ttctgcccgc
     7381 attgcgacgg tccgttctat aaaggcaagc atgtggctgt tgtaggcggc ggaaattcag
     7441 gcgttgaggc ggctatcgac ctggctggta tctgttcaaa agtgacgctt ctcgaatttg
     7501 ccgatgagtt gaaagcggat caggtcttgc aggataaggc aagaagcctt ccgaacgtgg
     7561 agatctttat gcattcccag acgatggagg tactgggtaa cggtgaaaaa gtgaccggta
     7621 tccgtataaa ggaccgtaag acagaagaag tccgcaacat cgaactggat ggcatctttg
     7681 tccagatcgg cctggcagcc aacagcggtc cgttccgtga gg
//
