LOCUS       c00109_gnlPro..         7649 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|LKGLEBJM_109.
ACCESSION   c00109_gnlPro..
VERSION     c00109_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-05 17:29:25
            Original ID  :: gnl|Prokka|LKGLEBJM_109
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 7649
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..7649
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[3581:6573]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      3582..6573
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..7649
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..7649
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             217..3402
                     /locus_tag="ctg109_1"
                     /transl_table=11
                     /translation="MKNNHNLLMKMKNLEKFSGAIKRCVAIAMASMLFATTGFSLDTHP
                     VKAQEVSSSADKIEIPEISKDAFKKYKKISGIGANTILGADFTYYQQCLEWGKSYKNYM
                     SQSVDNIFDYVKSQGINTISLKVAVNPTGENAYLSLENAIKTLKAVKASNTNLKTNLVL
                     LYSDEITYAGTQNLPADWEKAEKEEQSVTRVESAKTYTRETIAKLKQANVLPDIVTIGN
                     EVNWNFLGITDGEGWEGWKAMGDISALLKKEGVKNAVSIAAQPDAASVKYIVQKLGYAS
                     VDYDYIGVNVYPDNNTNSYIKSLKNEVESCAPDKQLIVSNVEYERVNEANTANVYTQAD
                     SIYNLLEATIDEKNAGGLIYNEAAYVGSWKSFFDDEGDAQVSMAIFAYAQGNETDTSRD
                     PYKYGDDTGLKQQKVTIKKVKNMSDSTIRGIDISSYTALKKAGVKYYDNEGKEASLLKV
                     LSDNGVNYIRIRIWNDPYNEKGETYGGGSNDVKAGLEIAKEAAKYNIKVLLGFHYSDFW
                     ADPAVQLLPKAWEKDRNNQEKMCSNVYEFTKETLEQFKDAGADIGMVQVGNEISQGMMG
                     IMHRTKANVWQEEEKSVLIDSYLNAGARAVRECVPDALVAIHLDTLNLSIYKDAMNAWE
                     RDKVDYDVLGSSSYAFWAGKNMLGNVRKAGNYVASRGKLFAVLETSWLNSQKDADGTVN
                     MVNNTKDAVYKVGPQGQADMLSDLYDAILSNDNGLGAFYWEGAWIPVKAGWVNWKYNKE
                     MANEFGTGWATENAGGYYPKSKLYYNGNPVWGGDSWDNQTLFDDKGYPLDSLRFYKDAV
                     SSNEKYSRVVIALCDKENNVLEYRVVKVISGKSMTYTLPEIKGYTKEKDTIKILGTNDK
                     ISKVSVVYNKDIKKQTITVKKASYTIPYGTKFNLKNEVKAVGSLTFTSNNTNIVSVEKQ
                     GKKLVVKKPGKVKIKITAGATADYKQTSRIVTIYAVPKKQTIKKVSTAKRKVKVNIKKD
                     VKATGYQIVAAKNSRFSKGKKVLTKKGTRQVTYTITKLNSRKIYYVKARAYKTIGNKKY
                     FGPWSKVKKIRIK"
     CDS             complement(3582..4334)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 76.2;
                     E-value: 1e-23)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg109_2"
                     /sec_met_domain="Radical_SAM (E-value: 1.9e-33, bitscore:
                     103.9, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTECNKNAMALVHSTESFGSVDGPGVRFIVFLQGCPLRCQFCHNP
                     DTWKMTEENGAVWRSADELLNQALRYRTYWKNSGGITVSGGEPLLQIDFMLEFFKKAKE
                     KGIHTVIDTAGGPFTREEPFFSKFQELMKYTDLLLVDIKHIDNKYHKVLTGRSNENILD
                     MIRYLSDIKKPIWIRHVLVPERSDKDEYLTRLADFIHSLDNVEKVEILPYHTMGIYKWK
                     ELGLEYPLEGIDPPTKERVENAKKILAI"
     CDS             complement(4321..6573)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate2"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg109_3"
                     /sec_met_domain="Gly_radical (E-value: 1.8e-42, bitscore:
                     132.0, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate2 (E-value: 8.7e-268, bitscore:
                     877.2, seeds: 33, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MLQKEQWQGFNGRIWREEINLREFIQDNYTPYDGDASFLEGPTEA
                     TDTLWGELQKLQKAEREKGGVLDMDTDIVSSLTSHKPGYISESLKEKEQIVGLQTDKPL
                     KRAFMPFGGIRTAEEACTTYGYTPNPEFHKIFTDYHKTHNQAVFDSYTPEMKKARHNKI
                     ITGLPDTYGRGRIVGDYRRVALYGIDLLVADKQKDFANCGDGTMTDDVIRLREEISMQI
                     KALKDMKVMAESYGYDISEPASNAKEAVQWTYFGYLAAIKTQNGAAMSIGRIATFLDIY
                     IKRDMDKGILTEKEAQELIDHMTMKFRMVKFGRIPAYNQLFSGDPVWATLEVGGLGQDG
                     RSMVTKTDYRFLHTLENMGPSPEPNLTVLYSERLPKAFRDYAAHISITTSSIQYENDDV
                     MRPVWGDDYSICCCVSATQTGKEMQFFGARANLAKCLLYAINGGVDEKTGQQVGPEYKP
                     ITSEYLDYDEVMHKYDIMMDWLAGLYVNTLNLIQYMHDKYYYEYALMALIDTNVRRTFA
                     TGIAGFSHVVDSLSAIKYAKVKTVRNEEGLVVDYETTGDFPKYGNDDDRADDIAVWLLQ
                     TFMKKLEKFHTYRDSEPTTSILTITSNVVYGKATGSLPDGRKAGEPLSPGANPSYGAEQ
                     NGLLASLNSVAKLPYEWALDGISNTQTINPDALGHSEEERIGNLVNVMDGYFAQGAHHL
                     NVNVFGVEKLIDAMEHPEKEEYANFTIRVSGYAVKFISLTKEQQLDVIARTCHDRM"
ORIGIN
        1 ctttaactga ggaggaaaac tcagaatcta ttttctgcaa ttgaaataga agttttcgga
       61 taggatgaat tcggaaagcg aggggaagat ttgataaaga aattagagta tgtttaaaaa
      121 atatcaaaaa tttaccaatt ttactatagt gtatgcttga aaaaagatta taatatgtta
      181 aaatatatat tattatttat aagaaagggg aaacttatga aaaacaatca taatctacta
      241 atgaaaatga agaatcttga aaaatttagc ggggctatca aaagatgtgt agcaatagca
      301 atggcatcca tgttatttgc aacaactgga tttagtttgg atacacatcc ggttaaggcg
      361 caagaagtaa gttcgtcagc agataagatt gaaattccag aaataagcaa ggatgctttt
      421 aagaagtaca aaaagatttc tggaattgga gcgaatacta ttttaggagc ggattttact
      481 tattatcagc agtgtctgga gtgggggaaa tcatataaaa attatatgag tcagtccgta
      541 gacaatattt tcgactatgt aaaaagtcag ggtatcaata ccatatcttt aaaggttgct
      601 gtaaatccaa caggggaaaa tgcatatctt tctttggaaa atgcaataaa aacattaaaa
      661 gctgtaaaag catctaatac aaatcttaag acaaatcttg tcttacttta ttcagacgaa
      721 ataacttatg cgggtacaca gaatcttccg gcagactggg agaaagcaga gaaagaagag
      781 caatcggtaa ctcgagtaga aagtgcaaaa acttatacaa gagagacaat tgcaaagtta
      841 aagcaggcga atgtccttcc tgatattgta acgattggta atgaggtgaa ttggaatttc
      901 cttggaatta ctgacggaga aggctgggaa ggctggaaag caatgggcga tatttcagca
      961 ttgctgaaaa aagaaggtgt aaagaatgct gtcagcatag ctgctcaacc tgatgctgct
     1021 tctgtaaaat atattgttca gaaactgggc tatgcgtctg tagattatga ttatattggg
     1081 gtaaatgttt atccagataa taatacaaac agttatatta aaagtttaaa aaacgaagta
     1141 gaaagttgtg caccggataa acaactgata gtttctaacg tagaatatga acgtgtaaat
     1201 gaggcgaata cagccaatgt atatacacag gctgacagca tttataactt gttagaagcc
     1261 acaattgatg agaagaatgc tggtggtttg atttataatg aagctgcata tgttggaagt
     1321 tggaagtctt tctttgatga tgagggagat gcacaggttt ccatggctat ttttgcatat
     1381 gcacagggta atgagacaga tacaagcaga gatccttata aatacggaga tgatactggt
     1441 ttaaaacagc agaaagtaac cattaaaaaa gtgaagaata tgtcagattc tactattcga
     1501 ggcatagaca tttcatcgta tactgcttta aaaaaggctg gggtaaaata ttatgataat
     1561 gaaggaaaag aagcctcatt actaaaagtt ttaagtgata atggtgtaaa ttacattcgt
     1621 attcgtatat ggaatgatcc gtataatgaa aaaggagaga cttatggtgg tggatccaat
     1681 gatgttaagg ctgggttaga aatagcgaaa gaggcagcca aatataatat taaagtttta
     1741 ttgggattcc attattctga tttttgggca gatccagcag tacagttgct tccaaaagca
     1801 tgggaaaaag atagaaataa tcaggaaaaa atgtgttcta atgtatatga atttactaaa
     1861 gaaaccttag aacaatttaa agatgctggt gctgacattg gtatggtaca agttggaaat
     1921 gaaatatctc agggaatgat ggggattatg catagaacta aagccaatgt ctggcaggaa
     1981 gaagaaaagt ctgtgcttat tgacagttat ttaaatgcag gtgccagggc agtaagagaa
     2041 tgtgtgccag atgctttggt tgcgatacat ttagatactc ttaatttaag tatatataaa
     2101 gatgctatga atgcatggga aagagataaa gtggattatg atgttttagg atcttctagc
     2161 tatgcctttt gggctggaaa aaatatgctt gggaatgttc ggaaagctgg aaattatgtt
     2221 gcatccagag gtaaattatt tgcagtattg gagacatcat ggcttaactc tcagaaagat
     2281 gcagatggta ctgtaaatat ggttaataat actaaagatg cagtttataa agttggtcct
     2341 cagggacagg cagatatgtt gtcagattta tatgatgcaa ttttatcaaa tgataatgga
     2401 ttgggtgctt tttattggga aggggcatgg atacctgtca aagctggttg ggtgaattgg
     2461 aaatataaca aagaaatggc caatgaattt ggaacaggat gggcgacaga aaatgcagga
     2521 ggatactatc caaagagtaa attgtattat aatgggaatc ctgtttgggg aggagattct
     2581 tgggataatc aaacgctttt tgatgataaa ggatacccat tagattctct tcgcttctat
     2641 aaagatgcag tgtcatctaa cgagaaatat tccagagtgg ttattgcatt atgtgataag
     2701 gaaaataatg tacttgaata cagggtagta aaagttattt caggaaagag tatgacttat
     2761 acattaccgg aaattaaagg atatacgaaa gaaaaagata caattaaaat tttaggaaca
     2821 aatgataaga tatctaaggt atctgtagtt tataataagg atataaaaaa acagacaatt
     2881 actgtgaaaa aagcatccta tacgattcct tatggaacaa agtttaatct taagaacgaa
     2941 gtaaaagcag ttggaagttt aacatttact tcaaataata cgaatattgt ttctgttgaa
     3001 aagcagggta aaaaacttgt tgtaaagaag ccaggaaaag taaagattaa aataacagcc
     3061 ggtgcaacag cagattataa acagaccagt agaatagtta ctatttatgc agttccgaaa
     3121 aagcagacca tcaaaaaggt ttctacagct aagagaaaag taaaagtgaa tataaagaaa
     3181 gatgtaaaag ctacaggata tcagattgtt gcagctaaga actcaagatt tagcaagggc
     3241 aagaaggtat taacaaagaa aggaacaaga caggtaacat atacaataac aaaattaaat
     3301 tctaggaaaa tttactatgt aaaggcacgt gcttataaaa caataggcaa taagaaatat
     3361 tttggtccat ggagcaaagt aaagaaaata agaataaagt aaattccata gatcatttga
     3421 aaaattagaa tttaatttga gcgaatttta actgaagttt atatctaaag agcaccccat
     3481 attgtgactt caaggataga ttgaagatga ataagtattc aacgcaatct atcttcaaag
     3541 tcacaaatat ggggggtttt aggattctgt ttgtcggcaa atcaaatagc aagaattttc
     3601 ttggcgttct ccactcgttc tttcgttgga gggtcaattc cttctaaagg atattctaat
     3661 ccaagttctt tccatttata aatacccatc gtatgataag gcaggatttc caccttttcc
     3721 acattatcaa gagagtgaat gaaatcagct aatctagtca aatattcatc tttatcgctt
     3781 cgttccggga caaggacatg gcgaatccaa atcggttttt tgatatcgga aagatagcga
     3841 atcatatcaa gaatattctc attgctacga ccagttaaaa ctttgtggta tttgttatca
     3901 atgtgtttga tatccactaa taacaggtct gtatatttca tcaattcctg aaatttagaa
     3961 aagaaaggtt cctctcttgt aaaaggtcct ccagcagtat caataacagt gtggattcct
     4021 ttttcttttg cctttttaaa aaactctagc ataaaatcaa tctgtaaaag aggctcacca
     4081 ccacttacgg taataccgcc gctatttttc cagtaagtac gataacggag tgcctgattt
     4141 aacagctcat ccgcacttct ccacaccgca ccattttcct ctgtcatctt ccatgtatct
     4201 ggattgtgac agaactgaca tcgcaaagga caaccctgta aaaaaacaat aaaacgaact
     4261 cccggcccat ccaccgaacc aaaactctcc gtagaatgaa caagagccat cgcattttta
     4321 ttacattctg tcatggcatg ttctggcaat tacatctaac tgctgttctt ttgtcagaga
     4381 gatgaattta actgcataac ctgaaacacg gatcgtaaag tttgcgtatt cttctttttc
     4441 cggatgttcc atagcatcaa tcagtttctc aacaccaaat acatttacgt ttaggtggtg
     4501 agcaccctgt gcgaagtagc cgtccataac atttactaag ttaccaatac gttcttcttc
     4561 ggaatgaccg agtgcatcag ggttgattgt ctgcgtattg gaaataccat ctaatgccca
     4621 ttcataagga agtttagcta cagagtttaa ggaagctaag agaccgttct gctctgcacc
     4681 ataacttggg tttgctcctg gggaaagtgg ttcaccggct tttcttccat ctggaaggga
     4741 acctgttgcc ttaccatata ctacgttaga tgtgatagta aggatagaag ttgtaggttc
     4801 agaatcacga taagtatgga acttctccag tttcttcatg aatgtctgaa gtaaccatac
     4861 agcgatatca tcggcgcggt cgtcatcatt tccgtattta gggaagtcgc cagttgtttc
     4921 ataatctaca acgagtcctt cttcgttacg aactgtctta acctttgcat atttgatagc
     4981 acttaaggag tctactacgt gtgagaatcc tgcgatacct gtagcgaatg tacgacgtac
     5041 atttgtatcg attaaggcca tcaaagcata ttcatagtaa tatttgtcat gcatgtactg
     5101 gatgagattt aatgtattta catataaacc tgctaaccag tccatcatga tatcatattt
     5161 gtgcattact tcatcatagt caagatattc ggaagtaatc ggtttatatt ccggtccaac
     5221 ctgctgtccg gtcttttcat ccacaccacc attgatagcg taaaggagac attttgcaag
     5281 gttagcgcga gcaccgaaga actgcatttc cttaccagtc tgtgtagcag aaacacagca
     5341 gcagatggag taatcatcgc cccatactgg acgcattaca tcatcatttt catactggat
     5401 tgaacttgtt gtaatagaaa tatgagctgc gtaatctcta aatgcttttg gaagacgctc
     5461 agagtaaagt actgtgaggt ttggctctgg ggatggtccc atattttcta atgtgtggag
     5521 gaaacggtag tcagtttttg taaccatgga acgaccgtcc tgtccaagtc cgccaacttc
     5581 aagtgttgcc catacagggt caccggagaa gagctggttg taagctggga tacgtccgaa
     5641 cttaaccata cggaacttca tagtcatatg gtcgattaat tcctgcgctt ctttctctgt
     5701 aaggattccc ttatccatat cacgtttgat ataaatgtca aggaaagtag cgatacgtcc
     5761 gatactcata gcagcaccgt tctgtgtctt gattgctgca aggtatccga aatacgtcca
     5821 ttgaacagct tcctttgcgt tagaagcagg ttcggaaata tcatagccat agctttcagc
     5881 cataactttc atatctttta atgctttaat ctgcatggag atttcttctc ttaaacggat
     5941 aacgtcatct gtcattgttc cgtcaccgca gtttgcgaaa tctttttgct tgtcagcaac
     6001 taacaggtca ataccgtaaa gagctacacg acggtagtca ccaacgatac gtccacgtcc
     6061 gtaagtatct ggaagaccag taatgatctt attgtgacga gctttcttca tttcaggagt
     6121 ataagaatca aatactgcct ggttatgtgt cttatgataa tctgtaaaaa tcttgtggaa
     6181 ttctggattt ggtgtgtaac cataagttgt acaagcttct tcagcagtac ggataccacc
     6241 aaatggcata aatgcacgtt ttaaaggttt atctgtctga agacctacga tctgttcttt
     6301 ctcttttaaa gactcagaaa tgtaacccgg tttatgggaa gtaaggcttg atacgatatc
     6361 agtatccata tcaagaacac cacccttttc acgctctgct ttctgcagct tctgcagttc
     6421 gccccacagt gtgtcagtag cttcggtagg accttcgagg aaagaagcat ctccatcata
     6481 tggagtgtag ttatcctgga tgaactctct taaattaatt tcttctctcc agattcgtcc
     6541 attaaatccc tgccattgtt ctttttgtaa catcttaatt gacctccctg tttgttaaaa
     6601 aaatccttaa gtcaaggttt tgaaatatgt ttgctaaaaa atcaacaaaa ttgttcagat
     6661 gatttactat gattagtttt aacttattta agttttaatc atctaacaac attattaaca
     6721 tgatttttgg caatcgtcaa acaatttttt gatattatgt gttctttttt ttgaacatat
     6781 acacaaaaat gcttgtagag cctttaaaat aaagggtttt agagcgttga taaaatatta
     6841 tcaaatcgta caaatgtact gatataagta caataatgac aagcatacaa ctataatttt
     6901 atttttcgct ctttagagca tgtttggaaa aacatcttct ttagcacatg acttaataac
     6961 gatttaaagt tacttcttat cagggatatt ggatagaata ttatcttatc agaaataaaa
     7021 aagccagcat ccagacaaga attgcccaga cggtcggcta taagagtctt atacttcatg
     7081 tggttcattc cacttacttc cgtcagtcgt ataagtatcc taactataaa caaaatagaa
     7141 caaaatgtca aggggttact atttaattca ttaagagaac tacagacgaa gccattgcct
     7201 ccaataaggc atatttgtga ttttagtcgc ggcgcgttga atgttcgctc catgcgctcg
     7261 catccaacac ttgctccgag accacaaata tgccttattg gaggcaatgg cttctgtgct
     7321 atggctggag aagatgaatt aaacagaggg aggggatttg gggacagaaa ctgatgtctt
     7381 ctgcttactc aagaatgact gggaacaggg ggatgattta gcttttttaa tggttatctc
     7441 agcaatattt caataatttc agaataaaaa gcagactgtt gttatcaata tcaattcatt
     7501 acctcttcta tttaaataca aaacatctat tcacaatatt ttaagagcaa gtagaaaact
     7561 tctaccctct cccccaaatc cccacaacca ctatttaatt caaacaaaat ccagccaaag
     7621 acagcccggg aagagaaccg ataccctat
//
