LOCUS       c00148_gnlPro..         6594 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|GDFJMIIP_148.
ACCESSION   c00148_gnlPro..
VERSION     c00148_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-05 15:31:17
            Original ID  :: gnl|Prokka|GDFJMIIP_148
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 6594
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..6594
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[65:3057]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      66..3057
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..6594
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..6594
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             complement(66..818)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 73.6;
                     E-value: 6.2e-23)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg148_1"
                     /sec_met_domain="Radical_SAM (E-value: 3.2e-32, bitscore:
                     100.7, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTECNKNSMALVHSTESFGSVDGPGVRFIVFLQGCPLRCQFCHNP
                     DTWKMTEENGAVWKSADALLNQALRYRPYWKNGGGITVSGGEPLLQIDFMLEFFKKAKE
                     KGIHTTIDTAGGPFTREEPFFSKFQELMKYTDLLLVDIKHIDNKYHKVLTGRSNEHILD
                     MIRYLSDIKKPIWIRHVLVPERSDKDEYLTRLADFIHSLDNVEKVEILPYHTMGIYKWK
                     ELGLEYPLEGIEPPTKERVENAKKILAI"
     CDS             complement(805..3057)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate2"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg148_2"
                     /sec_met_domain="Gly_radical (E-value: 3e-42, bitscore:
                     132.0, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate2 (E-value: 9.4e-268, bitscore:
                     877.8, seeds: 33, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MLQKEQWQGFNGRIWREEINLREFIQDNYTPYDGDASFLEGPTEA
                     TDTLWGELQKLQKAEREKGGVLDMDTDIVSSLTSHKPGYISESLKEKEQIVGLQTDKPL
                     KRAFMPFGGIRTAEEACTTYGYTPNPEFHKIFTDYHKTHNQAVFDSYTPEMKKARHNKI
                     ITGLPDTYGRGRIVGDYRRVALYGIDLLVADKQKDFANCGDGTMTDDVIRLREEISMQI
                     KALKDMKVMAESYGYDISEPASNAKEAVQWTYFGYLAAIKTQNGAAMSIGRIATFLDIY
                     IKRDMDKGILTEKEAQELIDHMTMKFRMVKFGRIPAYNQLFSGDPVWATLEVGGLGQDG
                     RSMVTKTDYRFLHTLENMGPSPEPNLTVLYSERLPKTFRDYAAHISITTSSIQYENDDV
                     MRPVWGDDYSICCCVSATQTGKEMQFFGARANLAKCLLYAINGGVDEKTGQQVGPEYKP
                     ITSEYLDYDEVMHKYDIMMDWLAGLYVNTLNLIQYMHDKYYYEYALMALIDTNVRRTFA
                     TGIAGFSHVVDSLSAIKYAKVKTVRNEDGLVVDYETTGDFPKYGNDDDRADDIAVWLLQ
                     TFMKKLEKFHTYRDSEPTTSILTITSNVVYGKATGSLPDGRKAGEPLSPGANPSYGAEQ
                     NGLLASLNSVAKLPYEWALDGISNTQTINPDALGHSEEERIGNLVNVMDGYFAQGAHHL
                     NVNVFGVEKLIDAMEHPEKEEYANFTIRVSGYAVKFISLTKEQQLDVIARTCHDRM"
     CDS             complement(3809..4243)
                     /locus_tag="ctg148_3"
                     /transl_table=11
                     /translation="MILQFFAAFIGATGFGFLFHLQFKHIFPAALGGVLTWVIYVIATT
                     WGFDVFISSLFASAFAAIYSEVIAKIRKAPTTLFLIISVVVLIPGGSLYYTMSYAVQKK
                     WAEAAAYGSQTLQCALGLAIGMSLIWIFHDMARKVYYLIH"
     CDS             complement(4240..5001)
                     /locus_tag="ctg148_4"
                     /transl_table=11
                     /translation="LNSPNAILHELLNLAEIMLTNGAEVSRVEETLNRMGHAYGTTQMN
                     VFAITSSIVVTMVFEEGEEYTQTRRITTPVGTDFFKLEQANALSRRCCSSPLSLPDLKD
                     EINKISKITPAPYTIYLGSMLGAGGFSVFFGGTILDGAIAVIFAFLLCFLQRHLNSICS
                     NTLILQFFCSLFVGSGICVAAHFFPVIHADKVMIGDIMLLIPGILMTNSIRDILIGDTI
                     SGVMRLVESLLWAGALACGFMCAIWFVGGVL"
     CDS             5067..6419
                     /locus_tag="ctg148_5"
                     /transl_table=11
                     /translation="LSYTWYEVFWFFLLYSFAGWVIGTSGAALKEKKFIDVGFLFGPWC
                     PSYGFGAVGFALFLPELKEQPVFLFAGGVILSFIITSLTGLTLERIFHQKWKDYSRRRF
                     NFGGYVNLPYTVVWGAAALLCIWIVNPFLSKIIGILPYGLGKVILIIAYVVIVIDFIGT
                     ISGILSVHIRLARLGKLVLVEEVADNLQKAADYMGNGLTGWVLKHLEKSHEGLEIKEII
                     RSKLQRREKAQEMTGVFAAGCGFYKLFWLFFLGSFLGDITETIFCWWKMGAITSRSSVV
                     YGPFSLVWGIGCFFLTYMLYQYRDRSDSFIFIFGTVLGGAYEYICSVVLELVFGTVFWD
                     YSKIPFNLGGRINLLYCFFWGIAAVVWMKILYPKLSGFIEKIPKKIGVPLTWFLVVFMV
                     FDMAISGLALNRYHERHQKAATPENAITKILDTRFPDKRMERIYPSAKHVK"
ORIGIN
        1 attcaacgca atctatcttc aaagtcacaa atatgggggg ttttaggatt ctgtttgtcg
       61 gcaaatcaaa tagcaagaat tttcttggcg ttctccactc gttctttcgt tggaggttca
      121 attccttcta aaggatattc taatccaagt tctttccatt tataaatacc catcgtatga
      181 taaggcagga tttccacctt ttccacatta tcaagagagt gaatgaaatc agctaatcta
      241 gtcagatatt catctttatc gcttcgttcc gggacaagga catggcgaat ccaaatcggt
      301 tttttgatat cggaaagata gcgaatcata tcaagaatat gttcattgct acgaccagtt
      361 aaaactttgt ggtatttgtt atcaatgtgt ttgatatcca ctaataacag gtcagtatat
      421 ttcatcaatt cctgaaattt agaaaagaaa ggttcttctc tcgtaaaagg acctccggca
      481 gtatcaatag tagtgtggat tcctttttct tttgcttttt taaaaaattc aagcataaaa
      541 tcaatctgta aaagaggctc tccgccactg acagtaatac ctccgccatt tttccagtaa
      601 ggacgatagc ggagtgcctg atttaacagc gcatctgcgc ttttccatac cgcaccattt
      661 tcttctgtca tcttccatgt atctggattg tgacagaact ggcaccgcaa agggcaaccc
      721 tgcaaaaaaa caataaaacg aactcccggc ccatccaccg aaccaaaact ctccgtagaa
      781 tgaacaagag ccatcgaatt tttattacat tctgtcatgg catgttctgg caattacatc
      841 taactgctgt tcttttgtca gagagatgaa tttaactgca taacctgaaa cacggatcgt
      901 aaagtttgcg tattcttctt tttccggatg ttccatcgca tcaatcagtt tctcaacacc
      961 aaatacattt acgtttaagt ggtgagcacc ctgtgcgaag tagccgtcca taacatttac
     1021 taagttacca atacgttctt cttcggaatg accgagtgca tcagggttga ttgtctgcgt
     1081 attggaaata ccatctaatg cccattcata aggaagttta gctacagagt ttaaggaagc
     1141 taagagaccg ttttgctctg caccataact tgggtttgct cctggggaaa gtggttcacc
     1201 ggcttttctt ccatctggaa gggaacctgt tgccttacca tatactacgt tagatgtgat
     1261 agtaaggata gaagttgtag gttcagaatc acgataagta tggaacttct ccagtttctt
     1321 catgaatgtc tgaagtaacc atacagcgat atcatcggcg cggtcgtcat catttccgta
     1381 tttagggaag tcgccagttg tctcataatc aacaacaaga ccatcttcat tacgaactgt
     1441 cttaaccttt gcatatttga tagcacttaa ggagtctact acatgtgaga atcctgcgat
     1501 acctgtagcg aatgtacgac gtacatttgt atcaattaag gccatcaaag cgtattcata
     1561 gtaatacttg tcatgcatgt actggatgag atttaatgta tttacatata aacctgctaa
     1621 ccagtccatc atgatatcat atttgtgcat tacttcatca tagtcaagat attcggaagt
     1681 aatcggttta tattccggtc caacctgctg tccggtcttt tcatccacac caccattgat
     1741 agcgtaaagg agacattttg caaggttagc gcgagcaccg aagaactgca tttccttacc
     1801 agtctgtgta gcagaaacac agcagcagat ggagtaatca tcgccccata ctggacgcat
     1861 tacatcatca ttttcatact ggattgaact tgttgtaata gaaatatgag ctgcgtaatc
     1921 tctaaatgtt tttggaagac gctcagagta aagtactgtg aggtttggct ctggggatgg
     1981 tcccatattt tctaatgtgt ggaggaaacg gtagtcagtt tttgtaacca tggaacggcc
     2041 gtcctgtcca agtccgccaa cttcaagtgt tgcccataca gggtcaccgg agaaaagctg
     2101 gttgtaagct gggatacgtc cgaacttaac catacggaac ttcatagtca tatggtcgat
     2161 taattcctgc gcttctttct ctgtaaggat tcccttatcc atatcacgtt tgatataaat
     2221 atcaaggaaa gtagcgatac gtccaatact catagcagca ccgttctgtg tcttgattgc
     2281 tgcaaggtat ccgaaatacg tccactgaac agcttccttt gcgttagaag caggttcgga
     2341 aatatcatag ccatagcttt cagccataac tttcatatct tttaatgctt taatctgcat
     2401 ggagatttct tctcttaaac ggataacgtc atctgtcatt gttccgtcac cgcagtttgc
     2461 gaaatctttc tgcttatcag caactaacag gtcaataccg taaagagcta cacgacggta
     2521 gtcaccaacg atacgtccac gtccgtaagt atctggaaga ccagtaatga tcttgttgtg
     2581 acgagctttc ttcatttcag gagtataaga atcaaatact gcctgattat gcgtcttatg
     2641 ataatctgta aaaatcttgt ggaattctgg atttggtgta taaccataag ttgtacaagc
     2701 ttcctcagca gtacggatac caccaaatgg cataaatgca cgttttaaag gtttatctgt
     2761 ctgaagacct acgatttgtt ctttctcttt taaagactca gaaatgtaac ccggtttatg
     2821 ggaagtaagg cttgatacga tatcagtatc catatcaaga acaccaccct tttcacgctc
     2881 tgctttctgc agcttctgca gttcgcccca cagtgtgtca gtagcttcgg taggaccttc
     2941 gaggaaagaa gcatctccat catatggagt gtagttatcc tggatgaact ctcttaaatt
     3001 aatttcttct ctccagattc gtccattaaa tccctgccat tgttcttttt gtaacatctt
     3061 aattgacctc cctgtttgtt aaaaaaatcc ttaagtcaag gttttgaaat atgtttgcta
     3121 aaaaatcaac aaaattgttc agatgattta ctataattag ttttaactta ttcaagtttt
     3181 aatcatctaa caacattatt aacatgattt ttggcaatcg tcaaacaatt ttttgatatt
     3241 atgtgttctt ttttttgaac atatacacaa aaatgcttgt agtgccttta aaataaaggg
     3301 ttttagagcg ttgataaaat attatcaaat cgtacaaatg tactgatata agtacaataa
     3361 tgacaagcat acaactataa ttttattttt cgctctttag agcatgtttg gaaaaacatc
     3421 ttctttagca catgacttaa taacgattta aagttacttc ttatcaggga tattggatag
     3481 aatattatct tatcagaaat aaaaaagcca gcatccagac aagaattgcc cagacggtcg
     3541 gctataagag tctcatactt catgtggttc attccactta cttccgtcag tcgtataagt
     3601 atcctaacta taaacaaaat agaacaaaat gtcaaggggt tactatttaa ttcattaagc
     3661 gaactacaga cgaagccatt gccccgaata aggcatattt gtgattttag tcgcggcgtg
     3721 ttgaatgttc gctctactcg ctcacatcca acacttgctc cgagaccaca aatataggat
     3781 atcttctctc ttctcgggcg tctgtctctt aatgaattaa atagtaaacc ttccttgcca
     3841 tatcgtggaa tatccagatg aggctcattc caatggcaag tcctaaagca cactgtaatg
     3901 tttgggagcc gtaagcggct gcttctgccc attttttctg aacagcataa ctcatcgtgt
     3961 aatagagaga gccgccggga attagaacga caacggaaat gattaaaaat aaggtggtag
     4021 gggcttttct tatttttgcg atgacttcgg agtaaattgc ggcgaaagca ctggcaaata
     4081 gagaagaaat gaaaacatca aatccccagg tagttgcgat gacatagata acccaggtca
     4141 gtacaccacc taatgcggca gggaaaatgt gcttaaattg caaatgaaat aagaatccga
     4201 agccggtagc tccaataaag gctgcaaaaa attgtaatat cataacacac ctccaacaaa
     4261 ccagatagcg cacataaatc cacaggcaag tgctcccgcc caaagaagcg attctacaag
     4321 acgcatcacg cctgaaatcg tgtcaccgat taaaatgtca cggatagagt ttgtcataag
     4381 gattccggga atcagcagca taatgtcacc gatcataact ttgtctgcat gaattaccgg
     4441 aaagaaatgt gcagctacac aaatgccgga gccgacaaaa agggagcaga aaaactgcag
     4501 gattaaagta ttgctgcata tagagtttaa atgcctttgt aaaaagcaaa ggagaaaggc
     4561 aaagataacg gcaatggctc cgtctaaaat tgttcctcca aaaaagacag aaaagccacc
     4621 tgcaccaagc atgcttccca ggtaaatggt ataaggagca ggagtgattt ttgaaatttt
     4681 atttatctca tctttcaaat ctggaaggga aagaggagaa gaacaacagc gcctgctgag
     4741 tgcatttgcc tgttctaatt taaagaaatc tgtgccgaca ggggtagtga ttcttcgggt
     4801 ttgtgtatat tcttcgcctt cttcaaagac catagtgaca acgatacttg aagtgattgc
     4861 aaatacattc atctgggtgg taccgtaggc atgccccatt cggttcagcg tttcttccac
     4921 acggctgacc tcggcaccgt tggtcagcat aatttccgca agatttagta attcatgtaa
     4981 aatagcattt ggtgaattca acgtactttc tcctttcaca aaaatatgat atagtatatc
     5041 acaaattcac gaaaaggaga aaaattttga gctatacctg gtatgaagtg ttctggtttt
     5101 ttctgttata ttcctttgca ggatgggtaa taggaacttc tggagcagca ttaaaagaaa
     5161 aaaagtttat tgacgtagga tttttatttg gcccgtggtg tccatcctac ggatttggag
     5221 ctgttggttt tgcattattt ttaccagaat taaaagaaca gcctgtattt ttatttgcag
     5281 gaggagtgat tctttcattc attatcacat ctcttacagg actgacactg gagcgtattt
     5341 tccatcagaa atggaaggat tattccagac ggagatttaa ttttggtggc tatgttaact
     5401 taccttatac ggtagtatgg ggtgcggcgg cactgttgtg tatttggatt gtaaatccat
     5461 ttttaagtaa aattattgga atacttccat atggattagg aaaagtcatt ttgattattg
     5521 cctatgtagt aattgttatt gattttatcg gaactatttc aggaattctg agtgtacata
     5581 tccgcctggc acgattagga aaactagtcc ttgtagaaga agtagctgat aacttacaaa
     5641 aagctgcaga ttacatggga aatggtctga ctggctgggt attaaaacat cttgaaaaaa
     5701 gtcatgaggg tctcgaaatc aaggaaatca tccgaagtaa actgcaaaga cgagaaaaag
     5761 cacaggaaat gactggtgta tttgcagcag gatgtggatt ttacaaactg ttctggctgt
     5821 tctttcttgg ctcttttctt ggcgatatta cagaaactat cttttgctgg tggaagatgg
     5881 gcgctatcac aagccgaagc agtgtagttt atggtccatt tagccttgtc tggggaattg
     5941 gctgtttctt cctcacatac atgctctacc aatatcgcga tagaagcgac agcttcatct
     6001 ttatattcgg aacagtcctc ggcggtgctt acgaatatat atgtagtgtt gtattagagc
     6061 ttgtctttgg aaccgtattc tgggattaca gcaaaattcc atttaatctg ggtggaagaa
     6121 tcaacttatt gtactgtttc ttctggggaa tcgcagccgt agtttggatg aaaatattat
     6181 atccgaaact atcaggtttt attgagaaga taccaaagaa aatcggggta ccattaacct
     6241 ggttcttggt tgtattcatg gtattcgata tggcaatctc aggacttgca ttaaaccgtt
     6301 atcacgaaag acaccagaag gcagcaacac cggaaaatgc tataacgaag atattagata
     6361 cgagatttcc agataagaga atggagagaa tatatccaag tgcgaaacat gtgaagtagg
     6421 gaattctgat ttgcgtagca aaggagacgt ccacagggaa tataatatcc tatatttgtg
     6481 actttagttg cggcatgttg aatactcgct ctattcgctc gtatccaaca cttgctccga
     6541 ggccacaaat ataggatatt atattccctg tgggctgtgt tttggctgcc taag
//
