GCA_958346805.1 - 5 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

gnlProkkaEOMAMBIB_15 (original name was: gnl|Prokka|EOMAMBIB_15)

Region	Type	From	To	Most similar known cluster		Similarity
Region 15.1	ranthipeptide	28,972	50,560

gnlProkkaEOMAMBIB_16 (original name was: gnl|Prokka|EOMAMBIB_16)

Region	Type	From	To	Most similar known cluster		Similarity
Region 16.1	lanthipeptide-class-ii	1,723	24,353

gnlProkkaEOMAMBIB_25 (original name was: gnl|Prokka|EOMAMBIB_25)

Region	Type	From	To	Most similar known cluster		Similarity
Region 25.1	cyclic-lactone-autoinducer	27,355	38,426

gnlProkkaEOMAMBIB_32 (original name was: gnl|Prokka|EOMAMBIB_32)

Region	Type	From	To	Most similar known cluster		Similarity
Region 32.1	cyclic-lactone-autoinducer	17,872	35,189

gnlProkkaEOMAMBIB_74 (original name was: gnl|Prokka|EOMAMBIB_74)

Region	Type	From	To	Most similar known cluster		Similarity
Region 74.1	NRPS	1	14,405	dipeptide aldehydes	NRP	23%

Region	Type	From	To	Most similar known cluster	Similarity
Region 15.1	ranthipeptide	28,972	50,560
Region 16.1	lanthipeptide-class-ii	1,723	24,353
Region 25.1	cyclic-lactone-autoinducer	27,355	38,426
Region 32.1	cyclic-lactone-autoinducer	17,872	35,189
Region 74.1	NRPS	1	14,405	dipeptide aldehydes	NRP	23%

No secondary metabolite regions were found in these records:: gnlProkkaEOMAMBIB_1 (original name was: gnl|Prokka|EOMAMBIB_1); gnlProkkaEOMAMBIB_2 (original name was: gnl|Prokka|EOMAMBIB_2); gnlProkkaEOMAMBIB_3 (original name was: gnl|Prokka|EOMAMBIB_3); gnlProkkaEOMAMBIB_4 (original name was: gnl|Prokka|EOMAMBIB_4); gnlProkkaEOMAMBIB_5 (original name was: gnl|Prokka|EOMAMBIB_5); gnlProkkaEOMAMBIB_6 (original name was: gnl|Prokka|EOMAMBIB_6); gnlProkkaEOMAMBIB_7 (original name was: gnl|Prokka|EOMAMBIB_7); gnlProkkaEOMAMBIB_8 (original name was: gnl|Prokka|EOMAMBIB_8); gnlProkkaEOMAMBIB_9 (original name was: gnl|Prokka|EOMAMBIB_9); gnlProkkaEOMAMBIB_10 (original name was: gnl|Prokka|EOMAMBIB_10); gnlProkkaEOMAMBIB_11 (original name was: gnl|Prokka|EOMAMBIB_11); gnlProkkaEOMAMBIB_12 (original name was: gnl|Prokka|EOMAMBIB_12); gnlProkkaEOMAMBIB_13 (original name was: gnl|Prokka|EOMAMBIB_13); gnlProkkaEOMAMBIB_14 (original name was: gnl|Prokka|EOMAMBIB_14); gnlProkkaEOMAMBIB_17 (original name was: gnl|Prokka|EOMAMBIB_17); gnlProkkaEOMAMBIB_18 (original name was: gnl|Prokka|EOMAMBIB_18); gnlProkkaEOMAMBIB_19 (original name was: gnl|Prokka|EOMAMBIB_19); gnlProkkaEOMAMBIB_20 (original name was: gnl|Prokka|EOMAMBIB_20); gnlProkkaEOMAMBIB_21 (original name was: gnl|Prokka|EOMAMBIB_21); gnlProkkaEOMAMBIB_22 (original name was: gnl|Prokka|EOMAMBIB_22); gnlProkkaEOMAMBIB_23 (original name was: gnl|Prokka|EOMAMBIB_23); gnlProkkaEOMAMBIB_24 (original name was: gnl|Prokka|EOMAMBIB_24); gnlProkkaEOMAMBIB_26 (original name was: gnl|Prokka|EOMAMBIB_26); gnlProkkaEOMAMBIB_27 (original name was: gnl|Prokka|EOMAMBIB_27); gnlProkkaEOMAMBIB_28 (original name was: gnl|Prokka|EOMAMBIB_28); gnlProkkaEOMAMBIB_29 (original name was: gnl|Prokka|EOMAMBIB_29); gnlProkkaEOMAMBIB_30 (original name was: gnl|Prokka|EOMAMBIB_30); gnlProkkaEOMAMBIB_31 (original name was: gnl|Prokka|EOMAMBIB_31); gnlProkkaEOMAMBIB_33 (original name was: gnl|Prokka|EOMAMBIB_33); gnlProkkaEOMAMBIB_34 (original name was: gnl|Prokka|EOMAMBIB_34); gnlProkkaEOMAMBIB_35 (original name was: gnl|Prokka|EOMAMBIB_35); gnlProkkaEOMAMBIB_36 (original name was: gnl|Prokka|EOMAMBIB_36); gnlProkkaEOMAMBIB_37 (original name was: gnl|Prokka|EOMAMBIB_37); gnlProkkaEOMAMBIB_38 (original name was: gnl|Prokka|EOMAMBIB_38); gnlProkkaEOMAMBIB_39 (original name was: gnl|Prokka|EOMAMBIB_39); gnlProkkaEOMAMBIB_40 (original name was: gnl|Prokka|EOMAMBIB_40); gnlProkkaEOMAMBIB_41 (original name was: gnl|Prokka|EOMAMBIB_41); gnlProkkaEOMAMBIB_42 (original name was: gnl|Prokka|EOMAMBIB_42); gnlProkkaEOMAMBIB_43 (original name was: gnl|Prokka|EOMAMBIB_43); gnlProkkaEOMAMBIB_44 (original name was: gnl|Prokka|EOMAMBIB_44); gnlProkkaEOMAMBIB_45 (original name was: gnl|Prokka|EOMAMBIB_45); gnlProkkaEOMAMBIB_46 (original name was: gnl|Prokka|EOMAMBIB_46); gnlProkkaEOMAMBIB_47 (original name was: gnl|Prokka|EOMAMBIB_47); gnlProkkaEOMAMBIB_48 (original name was: gnl|Prokka|EOMAMBIB_48); gnlProkkaEOMAMBIB_49 (original name was: gnl|Prokka|EOMAMBIB_49); gnlProkkaEOMAMBIB_50 (original name was: gnl|Prokka|EOMAMBIB_50); gnlProkkaEOMAMBIB_51 (original name was: gnl|Prokka|EOMAMBIB_51); gnlProkkaEOMAMBIB_52 (original name was: gnl|Prokka|EOMAMBIB_52); gnlProkkaEOMAMBIB_53 (original name was: gnl|Prokka|EOMAMBIB_53); gnlProkkaEOMAMBIB_54 (original name was: gnl|Prokka|EOMAMBIB_54); gnlProkkaEOMAMBIB_55 (original name was: gnl|Prokka|EOMAMBIB_55); gnlProkkaEOMAMBIB_56 (original name was: gnl|Prokka|EOMAMBIB_56); gnlProkkaEOMAMBIB_57 (original name was: gnl|Prokka|EOMAMBIB_57); gnlProkkaEOMAMBIB_58 (original name was: gnl|Prokka|EOMAMBIB_58); gnlProkkaEOMAMBIB_59 (original name was: gnl|Prokka|EOMAMBIB_59); gnlProkkaEOMAMBIB_60 (original name was: gnl|Prokka|EOMAMBIB_60); gnlProkkaEOMAMBIB_61 (original name was: gnl|Prokka|EOMAMBIB_61); gnlProkkaEOMAMBIB_62 (original name was: gnl|Prokka|EOMAMBIB_62); gnlProkkaEOMAMBIB_63 (original name was: gnl|Prokka|EOMAMBIB_63); gnlProkkaEOMAMBIB_64 (original name was: gnl|Prokka|EOMAMBIB_64); gnlProkkaEOMAMBIB_65 (original name was: gnl|Prokka|EOMAMBIB_65); gnlProkkaEOMAMBIB_66 (original name was: gnl|Prokka|EOMAMBIB_66); gnlProkkaEOMAMBIB_67 (original name was: gnl|Prokka|EOMAMBIB_67); gnlProkkaEOMAMBIB_68 (original name was: gnl|Prokka|EOMAMBIB_68); gnlProkkaEOMAMBIB_69 (original name was: gnl|Prokka|EOMAMBIB_69); gnlProkkaEOMAMBIB_70 (original name was: gnl|Prokka|EOMAMBIB_70); gnlProkkaEOMAMBIB_71 (original name was: gnl|Prokka|EOMAMBIB_71); gnlProkkaEOMAMBIB_72 (original name was: gnl|Prokka|EOMAMBIB_72); gnlProkkaEOMAMBIB_73 (original name was: gnl|Prokka|EOMAMBIB_73); gnlProkkaEOMAMBIB_75 (original name was: gnl|Prokka|EOMAMBIB_75); gnlProkkaEOMAMBIB_76 (original name was: gnl|Prokka|EOMAMBIB_76); gnlProkkaEOMAMBIB_77 (original name was: gnl|Prokka|EOMAMBIB_77); gnlProkkaEOMAMBIB_78 (original name was: gnl|Prokka|EOMAMBIB_78); gnlProkkaEOMAMBIB_79 (original name was: gnl|Prokka|EOMAMBIB_79); gnlProkkaEOMAMBIB_80 (original name was: gnl|Prokka|EOMAMBIB_80); gnlProkkaEOMAMBIB_81 (original name was: gnl|Prokka|EOMAMBIB_81); gnlProkkaEOMAMBIB_82 (original name was: gnl|Prokka|EOMAMBIB_82); gnlProkkaEOMAMBIB_83 (original name was: gnl|Prokka|EOMAMBIB_83); gnlProkkaEOMAMBIB_84 (original name was: gnl|Prokka|EOMAMBIB_84); gnlProkkaEOMAMBIB_85 (original name was: gnl|Prokka|EOMAMBIB_85); gnlProkkaEOMAMBIB_86 (original name was: gnl|Prokka|EOMAMBIB_86); gnlProkkaEOMAMBIB_87 (original name was: gnl|Prokka|EOMAMBIB_87); gnlProkkaEOMAMBIB_88 (original name was: gnl|Prokka|EOMAMBIB_88); gnlProkkaEOMAMBIB_89 (original name was: gnl|Prokka|EOMAMBIB_89); gnlProkkaEOMAMBIB_90 (original name was: gnl|Prokka|EOMAMBIB_90); gnlProkkaEOMAMBIB_91 (original name was: gnl|Prokka|EOMAMBIB_91); gnlProkkaEOMAMBIB_92 (original name was: gnl|Prokka|EOMAMBIB_92); gnlProkkaEOMAMBIB_93 (original name was: gnl|Prokka|EOMAMBIB_93); gnlProkkaEOMAMBIB_94 (original name was: gnl|Prokka|EOMAMBIB_94); gnlProkkaEOMAMBIB_95 (original name was: gnl|Prokka|EOMAMBIB_95); gnlProkkaEOMAMBIB_96 (original name was: gnl|Prokka|EOMAMBIB_96); gnlProkkaEOMAMBIB_97 (original name was: gnl|Prokka|EOMAMBIB_97); gnlProkkaEOMAMBIB_98 (original name was: gnl|Prokka|EOMAMBIB_98); gnlProkkaEOMAMBIB_99 (original name was: gnl|Prokka|EOMAMBIB_99); gnlProkkaEOMAMBIB_100 (original name was: gnl|Prokka|EOMAMBIB_100); gnlProkkaEOMAMBIB_101 (original name was: gnl|Prokka|EOMAMBIB_101); gnlProkkaEOMAMBIB_102 (original name was: gnl|Prokka|EOMAMBIB_102); gnlProkkaEOMAMBIB_103 (original name was: gnl|Prokka|EOMAMBIB_103); gnlProkkaEOMAMBIB_104 (original name was: gnl|Prokka|EOMAMBIB_104); gnlProkkaEOMAMBIB_105 (original name was: gnl|Prokka|EOMAMBIB_105); gnlProkkaEOMAMBIB_106 (original name was: gnl|Prokka|EOMAMBIB_106); gnlProkkaEOMAMBIB_107 (original name was: gnl|Prokka|EOMAMBIB_107); gnlProkkaEOMAMBIB_108 (original name was: gnl|Prokka|EOMAMBIB_108)

Compact view

gnlProkkaEOMAMBIB_15 - Region 1 - ranthipeptide

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Location: 28,972 - 50,560 nt. (total: 21,589 nt) Show pHMM detection rules used

Region on contig edge.

ranthipeptide: (TIGR03973 or SCIFF or (cds(PF04055 and (TIGR04085 or SPASM)) and minimum(1, [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE, Cytolysin_C_RRE, Goadsporin_CD_RRE, Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE, Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE, Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE, Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE, Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE, Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE, Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE, Thiaglutamate_B_RRE, Thiaglutamate_G_RRE, Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE, Thurincin_rSAM_RRE, Trifolitoxin_RRE]) and not (TIGR01716 or subtilosin or TIGR02111 or TIGR03966 or TIGR04103 or (YcaO or TIGR03604))))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

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Gene overview

KnownClusterBlast

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Similar known gene clusters from MIBiG 3.1

No matches found.

gnlProkkaEOMAMBIB_16 - Region 1 - lanthipeptide-class-ii

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Location: 1,723 - 24,353 nt. (total: 22,631 nt) Show pHMM detection rules used

Region on contig edge.

lanthipeptide-class-ii: cds(LANC_like and DUF4135)

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

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resistance

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KnownClusterBlast

Lanthipeptides

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Similar known gene clusters from MIBiG 3.1

No matches found.

Lanthipeptide predictions

Lanthipeptide(s) for ctg16_11

allorf_03180_03365

Leader:

MSLNFSTYAYITKSPVRYSDAAVFSA

Core:

AAPDYHHPELFPVLLLFHTRELYPHMTEVTCFQTL

Core with dehydrations:

AAPDYHHPELFPVLLLFHDhbRELYPHMDhbEVDhbCysFQDhbL

antiSMASH-DB 4.0 matches for allorf_03180_03365

14.3% NZ_CP058552.1: Lanthipeptides: allorf_1481579_1481740_lanthipeptide

23

YPHMTEVTCFQTL

35

Y H+ + C QT+

6

YKHVQKTNCVQTI

18

allorf_18238_18366

Leader:

MLQLLTILLTPIYFRMHLQAG

Core:

QILTCLVVLMVIFIVTHYTCN

Core with dehydrations:

QILDhbCysLVVLMVIFIVDhbHYDhbCysN

Legend:

Dha: Didehydroalanine
Dhb: Didehydrobutyrine

Lanthipeptides

Core peptide predictions

allorf_03180_03365 - Class II

Cleavage pHMM score: -6.80

RODEO score: 0

Monoisotopic mass: 4093.1 Da

Molecular weight: 4095.7 Da

Number of bridges: 1

Alternative weights: (assuming N unmodified Ser/Thr residues); 4113.7 Da N = 1; 4131.7 Da N = 2; 4149.7 Da N = 3

allorf_18238_18366 - Class II

Cleavage pHMM score: -4.90

RODEO score: 0

Monoisotopic mass: 2368.3 Da

Molecular weight: 2369.9 Da

Number of bridges: 2

Alternative weights: (assuming N unmodified Ser/Thr residues); 2388.0 Da N = 1

gnlProkkaEOMAMBIB_25 - Region 1 - cyclic-lactone-autoinducer

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Location: 27,355 - 38,426 nt. (total: 11,072 nt) Show pHMM detection rules used

Region on contig edge.

cyclic-lactone-autoinducer: (TIGR04223 or AgrB)

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core biosynthetic genes

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KnownClusterBlast

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Similar known gene clusters from MIBiG 3.1

No matches found.

gnlProkkaEOMAMBIB_32 - Region 1 - cyclic-lactone-autoinducer

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Location: 17,872 - 35,189 nt. (total: 17,318 nt) Show pHMM detection rules used

Region on contig edge.

cyclic-lactone-autoinducer: (TIGR04223 or AgrB)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

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Gene details

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Gene overview

KnownClusterBlast

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Similar known gene clusters from MIBiG 3.1

No matches found.

gnlProkkaEOMAMBIB_74 - Region 1 - NRPS

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Location: 1 - 14,405 nt. (total: 14,405 nt) Show pHMM detection rules used

Region on contig edge.

NRPS: cds(Condensation and (AMP-binding or A-OX))

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core biosynthetic genes

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resistance

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NRPS/PKS domains

KnownClusterBlast

NRPS/PKS modules

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Detailed domain annotation

Selected features only

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Similar known gene clusters from MIBiG 3.1

Module view

Legend

NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 0 - 14405:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X - X - X)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

ctg74_4

AMP-binding (20..419): X

nrpys: Leu, Trp

SVM prediction details:

Predicted physicochemical class:: N/A
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Tiny, hydrophilic, transition to aliphatic (Gly, Ala)
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DVLTDGGISK
Nearest Stachelhaus code(s):: Leu or Trp DVLTDGGISK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (1033..1421): X

nrpys: Leu, Trp

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DAMFSGAIFK
Nearest Stachelhaus code(s):: Leu or Trp DAMFSGAIFK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (2027..2418): X

nrpys: Leu, Trp

SVM prediction details:

Predicted physicochemical class:: N/A
Large clusters prediction:: Aromatic side chain (Phe, Trp, Ph-Gly, Tyr, bOH-Tyr)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DAITTGGVSK
Nearest Stachelhaus code(s):: Leu or Trp DAITTGGVSK (100% 8Å match)
Stachelhaus code match:: 100% (strong)