LOCUS       c00106_gnlPro..         4702 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|GGJFDKMM_106.
ACCESSION   c00106_gnlPro..
VERSION     c00106_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-05 15:40:52
            Original ID  :: gnl|Prokka|GGJFDKMM_106
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 4702
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..4702
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[1105:4097]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      1106..4097
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..4702
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..4702
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             2..646
                     /locus_tag="ctg106_1"
                     /transl_table=11
                     /translation="ATKQELINLLEDYLGYKENHRVPKKYHLEIQDWPCHSLDSAYQTK
                     EDFHVCELYSAEWNAVRKEYGEKRTDHEETWEEIRIKQKDYANCEQKYKVLVHLEDEEG
                     NPLGNYNVMLCEYDTEKELIDLLEDYWGYKKNIYKEYYLKVIDWPHNPGPSEWWPGSSL
                     HVCEMEEREFRVIRKKYKEDDGPMPCLYGCPNSRDIEEKKEVETKIEIVYF"
     CDS             complement(1106..1858)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 75.7;
                     E-value: 1.5e-23)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg106_2"
                     /sec_met_domain="Radical_SAM (E-value: 4.3e-32, bitscore:
                     99.5, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTECNKNAMALVHSTESFGSVDGPGVRFIVFLQGCPLRCQFCHNP
                     DTWKMTEENGAVWKSADALLNQALRYRPYWKNGGGITVSGGEPLLQIDFMLEFFKKAKE
                     KGIHTVIDTAGGPFTREEPFFSKFQELMKYTDLLLVDIKHIDNKYHKVLTGRSNENILD
                     MIRYLSDIKKPIWIRHVLVPERSDKDEYLTRLADFIHSLDNVEKVEILPYHTMGIYKWK
                     ELGLEYPLEGIEPPTKERVENAKKILAI"
     CDS             complement(1845..4097)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate2"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg106_3"
                     /sec_met_domain="Gly_radical (E-value: 1.8e-42, bitscore:
                     132.0, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate2 (E-value: 8.7e-268, bitscore:
                     877.2, seeds: 33, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MLQKEQWQGFNGRIWREEINLREFIQDNYTPYDGDASFLEGPTEA
                     TDTLWGELQKLQKAEREKGGVLDMDTDIVSSLTSHKPGYISESLKEKEQIVGLQTDKPL
                     KRAFMPFGGIRTAEEACTTYGYTPNPEFHKIFTDYHKTHNQAVFDSYTPEMKKARHNKI
                     ITGLPDTYGRGRIVGDYRRVALYGIDLLVADKQKDFANCGDGTMTDDVIRLREEISMQI
                     KALKDMKVMAESYGYDISEPASNAKEAVQWTYFGYLAAIKTQNGAAMSIGRIATFLDIY
                     IKRDMDKGILTEKEAQELIDHMTMKFRMVKFGRIPAYNQLFSGDPVWATLEVGGLGQDG
                     RSMVTKTDYRFLHTLENMGPSPEPNLTVLYSERLPKAFRDYAAHISITTSSIQYENDDV
                     MRPVWGDDYSICCCVSATQTGKEMQFFGARANLAKCLLYAINGGVDEKTGQQVGPEYKP
                     ITSEYLDYDEVMHKYDIMMDWLAGLYVNTLNLIQYMHDKYYYEYALMALIDTNVRRTFA
                     TGIAGFSHVVDSLSAIKYAKVKTVRNEEGLVVDYETTGDFPKYGNDDDRADDIAVWLLQ
                     TFMKKLEKFHTYRDSEPTTSILTITSNVVYGKATGSLPDGRKAGEPLSPGANPSYGAEQ
                     NGLLASLNSVAKLPYEWALDGISNTQTINPDALGHSEEERIGNLVNVMDGYFAQGAHHL
                     NVNVFGVEKLIDAMEHPEKEEYANFTIRVSGYAVKFISLTKEQQLDVIARTCHDRM"
ORIGIN
        1 tgccacaaaa caggaattga taaatttatt agaggattat ctgggatata aagaaaatca
       61 tcgtgtccct aaaaaatatc atttagagat acaagattgg ccatgtcatt ctttagatag
      121 tgcatatcag acaaaagaag atttccatgt atgtgagctc tacagtgctg agtggaatgc
      181 tgtcagaaaa gaatatggtg agaaaagaac agaccatgaa gaaacatggg aagaaataag
      241 aataaaacaa aaagactatg caaactgcga acaaaaatat aaagtgcttg ttcatttaga
      301 agatgaagaa ggcaatccac tgggaaacta taatgtaatg ctgtgtgaat atgatacgga
      361 aaaagagtta atagatttat tagaagatta ttggggatat aaaaagaata tatataaaga
      421 gtattatcta aaagttatag attggccaca taatcctggt ccaagtgagt ggtggccggg
      481 tagtagttta catgtatgtg aaatggaaga gagagaattt cgtgttatca ggaaaaaata
      541 taaagaagac gatggtccaa tgccttgcct ttatggttgt ccgaattcaa gagatataga
      601 agaaaagaaa gaagtggaaa caaaaataga gattgtttat ttctgatttg tagtgctaaa
      661 cagacgccgt tgtttagggc aatgtagccg gctttttaga agtatttaga taggaaacat
      721 aaaacatagc ctattttgtt ccgttgtgct aaatgctcca ttttgcccag taaagcagga
      781 acttggcctc aaaatctcca ctggagcttt tttcgatctg ctaaggcagg agaagggagt
      841 cccaagttcc tatggtcaaa attccgcagc acaaagtcgc aaaataggct atgttttatg
      901 tttctttttc ctatattctt tgtaaaatcc ggaaaaaaat ttttgtaacg tctgtttctc
      961 taaatcttgc aaaaaatcct gcaaattaat ggaacaaaat acaaccttag aagaatccct
     1021 ctgttagaag tcatcgatgc aatccgacca taaatatctc cacagctgcg tttgtcatca
     1081 actttacaaa tcagaaattg ataaatcaga tagcaagaat cttctttgcg ttctccactc
     1141 gttcttttgt tggtggctca attccctcca gtggatactc taatccaagt tctttccatt
     1201 tataaattcc catcgtatga taaggcagga tttcaacctt ttccacatta tcaagagagt
     1261 gaatgaaatc agctaatcta gtcagatatt catctttatc gcttcgttcc gggacaagaa
     1321 catggcgaat ccaaatcggt tttttgatat cggaaagata gcgaatcata tcaagaatat
     1381 tctcgttgct gcgaccagtt aaaactttgt ggtatttgtt atcaatgtgt ttgatatcca
     1441 ctaataacag gtcagtatat ttcatcaatt cctgaaattt agaaaagaaa ggttcttctc
     1501 ttgtaaaagg tcctccagca gtatcaataa cggtgtggat tcctttttct tttgcttttt
     1561 taaaaaactc aagcataaaa tcaatctgta aaagaggctc tccaccactg acagtaatac
     1621 ctccgccatt tttccagtaa ggacgatagc ggagtgcctg atttaacagc gcatctgcgc
     1681 ttttccatac cgcaccattt tcttctgtca tcttccatgt atctggattg tgacagaact
     1741 ggcaccgcaa agggcaaccc tgcaaaaaaa caataaaacg aactcccggc ccatccaccg
     1801 aaccaaaact ctccgtagaa tgaacaagag ccatcgcatt tttattacat tctgtcatgg
     1861 catgttctgg caattacatc taactgctgt tcttttgtca gagagatgaa tttaactgca
     1921 taacctgaaa cacggatcgt aaagtttgcg tattcttctt tttccggatg ttccatcgca
     1981 tcaatcagtt tctcaacacc aaatacattt acgtttaagt ggtgagcacc ctgtgcgaag
     2041 tagccatcca taacatttac taagttacca atacgttctt cttcggaatg accgagtgca
     2101 tcagggttga ttgtctgcgt attggaaata ccatctaatg cccattcata aggaagttta
     2161 gctacagagt ttaaggaagc taagagaccg ttctgctctg cgccataact tgggtttgct
     2221 cctggggaaa gtggttcacc ggcttttctt ccatctggaa gggaacctgt tgccttacca
     2281 tatactacgt tagatgtgat agtaaggata gaagttgtag gttcagaatc acgataagta
     2341 tggaacttct ccagtttctt catgaatgtc tgaagtaacc atacagcgat atcatcggcg
     2401 cggtcatcat catttccata tttagggaag tcgccagttg tctcataatc tacaacgagt
     2461 ccttcttcgt tacgaactgt cttaaccttt gcatacttga tagcacttaa ggagtctact
     2521 acgtgtgaga atcctgcgat acctgtagcg aatgtacgac gtacatttgt atcgattaag
     2581 gccatcaaag cgtattcata gtagtatttg tcatgcatgt actggatgag atttaatgta
     2641 tttacatata aacctgctaa ccagtccatc atgatatcat atttgtgcat tacttcatca
     2701 tagtcaagat attcggaagt aatcggttta tattccggtc caacctgctg tccggtcttt
     2761 tcatccacac cgccattgat agcgtaaagg agacattttg caaggttagc gcgagcaccg
     2821 aagaactgca tttccttacc agtctgtgta gcagaaacac agcagcagat ggagtaatca
     2881 tcgccccata ctggacgcat tacatcatca ttttcatact ggattgaact tgttgtaata
     2941 gaaatatgag ctgcgtaatc tctaaatgct tttggaagac gctcagagta aagtactgtg
     3001 aggtttggct ctggggatgg tcccatattt tctaatgtgt ggaggaaacg gtagtcagtt
     3061 tttgtaacca tggaacgacc gtcctgtcca agtccgccaa cttcaagtgt tgcccataca
     3121 gggtcaccgg agaagagctg gttgtaagct gggatacgtc cgaacttaac catacggaac
     3181 ttcatagtca tatggtcgat taattcctgc gcttctttct ctgtaaggat tcccttatcc
     3241 atatcacgtt tgatataaat gtcaaggaaa gtagcgatac gtccgatact catagcagca
     3301 ccgttctgtg tcttgattgc tgcaaggtat ccgaaatacg tccactgaac agcttccttt
     3361 gcgttagaag caggttcgga aatatcatag ccatagcttt cagccataac tttcatatct
     3421 tttaatgctt taatttgcat ggagatttct tctcttaaac ggataacgtc atctgtcatt
     3481 gttccgtcac cgcagtttgc gaaatctttt tgcttgtcag caactaatag gtcaataccg
     3541 taaagagcta cacgacggta gtcaccaacg atacgtccac gtccgtaagt atctggaaga
     3601 ccagtaatga tcttattgtg acgagctttc ttcatttcag gagtataaga atcaaatact
     3661 gcctggttat gtgtcttatg ataatctgta aaaatcttgt ggaattctgg atttggtgtg
     3721 taaccataag ttgtacaagc ttcttcagca gtacggatac caccaaatgg cataaatgca
     3781 cgttttaaag gtttatctgt ctgaagacct acgatctgtt ctttctcttt taaagactca
     3841 gaaatgtaac ccggtttatg ggaagtaagg cttgatacga tatcagtatc catatcaaga
     3901 acaccaccct tttcacgctc tgctttctgc agcttctgca gttcgcccca cagtgtgtca
     3961 gtagcttcgg taggaccttc gaggaaagaa gcatctccat catatggagt gtagttatcc
     4021 tggatgaact ctcttaaatt aatttcttct ctccagattc gtccattaaa tccctgccat
     4081 tgttcttttt gtaacatctt aattgacctc cctgtttgtt aaaaaaatcc ttaagtcaag
     4141 gttttgaaat atgtttgcta aaaaatcaac aaaattgttc agatgattta ctgtaattag
     4201 ttttaactta tttaagtttt aatcatctaa caacattatt aacatgattt ttggcaatcg
     4261 tcaaacaatt ttttgatatt atgtgttctt ttttttgaac atatacacaa aaatgcttgt
     4321 acagccttta aaataaaggg ttttagagcg ttgataaaat attatcaaat cgtacaaatg
     4381 tactgatata agtacaataa tgacaagcat acaactataa ttttattttt cgctctttag
     4441 agcatgtttg gaaaaacatc ttctttagca catgacttaa taacgattta aagttacttc
     4501 ttatcaggga tattggatag aatattatct tatcagaaat aaaaaagcca gcatccagac
     4561 aagaattgcc cagacggtcg actataagag tcttatactt catgtggttc attccactta
     4621 cttccgtcag tcgtataagt atcctaacta taaacaaaat agaacaaaat agaaggaggc
     4681 tatgatggaa ctttatcagt tg
//
