LOCUS       c00089_gnlPro..         4535 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|AKOCODOI_89.
ACCESSION   c00089_gnlPro..
VERSION     c00089_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-05 17:50:23
            Original ID  :: gnl|Prokka|AKOCODOI_89
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 4535
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..4535
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[107:4352](+)"
                     /cutoff="10000"
                     /detection_rule="(minimum(1, [HAAA1, HG2B1, HG2B2, HGD-D,
                     HleuD1, HleuD2, HleuS1, HleuS2, Lactoyl-CoA1,
                     Lactoyl-CoA2]) and minimum(1, [ACP_syn_III, ACP_syn_III_C,
                     AcetylCoA_hyd_C, AcetylCoA_hydro, Acyl-CoA_dh_1,
                     Acyl-CoA_dh_2, AdoHcyase_NAD, AlaDh_PNT_C, BcrAD_BadFG,
                     Biotin_lipoyl, Carboxyl_trans, CoA_trans,
                     CoA_trans_230_acto, CoA_trans_6_Glutaconate, CoA_transf_3,
                     ECH_1, ECH_2, ETF, ETF_alpha, FGGY_C, HCDH, HCDH_N,
                     Hacid_dh, Hacid_dh_C, Mal_decarbox_Al, Peptidase_M22,
                     Peptidase_M23, Thiolase_C, Thiolase_N, ketoacyl-synt]) and
                     not (minimum(2, [AcetylCoA_hyd_C, BcrAD_BadFG, CoA_trans,
                     CoA_trans_230_acto, Mal_decarbox_Al]) and Lactoyl-CoA2 and
                     Lactoyl-CoA1) and not (minimum(4, [ACP_syn_III,
                     ACP_syn_III_C, Acyl-CoA_dh_1, Acyl-CoA_dh_2, AlaDh_PNT_C,
                     BcrAD_BadFG, ECH_1, ECH_2, ETF, ETF_alpha, HCDH, HCDH_N,
                     Hacid_dh, Hacid_dh_C, IlvN, NAD_Gly3P_dh_N, Thiolase_C,
                     Thiolase_N, ketoacyl-synt]) and HAAA1 and not HleuS1))"
                     /neighbourhood="10000"
                     /product="Others_HGD_unassigned"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      108..4352
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="10000"
                     /detection_rule="(minimum(1, [HAAA1, HG2B1, HG2B2, HGD-D,
                     HleuD1, HleuD2, HleuS1, HleuS2, Lactoyl-CoA1,
                     Lactoyl-CoA2]) and minimum(1, [ACP_syn_III, ACP_syn_III_C,
                     AcetylCoA_hyd_C, AcetylCoA_hydro, Acyl-CoA_dh_1,
                     Acyl-CoA_dh_2, AdoHcyase_NAD, AlaDh_PNT_C, BcrAD_BadFG,
                     Biotin_lipoyl, Carboxyl_trans, CoA_trans,
                     CoA_trans_230_acto, CoA_trans_6_Glutaconate, CoA_transf_3,
                     ECH_1, ECH_2, ETF, ETF_alpha, FGGY_C, HCDH, HCDH_N,
                     Hacid_dh, Hacid_dh_C, Mal_decarbox_Al, Peptidase_M22,
                     Peptidase_M23, Thiolase_C, Thiolase_N, ketoacyl-synt]) and
                     not (minimum(2, [AcetylCoA_hyd_C, BcrAD_BadFG, CoA_trans,
                     CoA_trans_230_acto, Mal_decarbox_Al]) and Lactoyl-CoA2 and
                     Lactoyl-CoA1) and not (minimum(4, [ACP_syn_III,
                     ACP_syn_III_C, Acyl-CoA_dh_1, Acyl-CoA_dh_2, AlaDh_PNT_C,
                     BcrAD_BadFG, ECH_1, ECH_2, ETF, ETF_alpha, HCDH, HCDH_N,
                     Hacid_dh, Hacid_dh_C, IlvN, NAD_Gly3P_dh_N, Thiolase_C,
                     Thiolase_N, ketoacyl-synt]) and HAAA1 and not HleuS1))"
                     /neighbourhood="10000"
                     /product="Others_HGD_unassigned"
                     /protocluster_number="1"
     cand_cluster    1..4535
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(minimum(1, [HAAA1, HG2B1, HG2B2, HGD-D,
                     HleuD1, HleuD2, HleuS1, HleuS2, Lactoyl-CoA1,
                     Lactoyl-CoA2]) and minimum(1, [ACP_syn_III, ACP_syn_III_C,
                     AcetylCoA_hyd_C, AcetylCoA_hydro, Acyl-CoA_dh_1,
                     Acyl-CoA_dh_2, AdoHcyase_NAD, AlaDh_PNT_C, BcrAD_BadFG,
                     Biotin_lipoyl, Carboxyl_trans, CoA_trans,
                     CoA_trans_230_acto, CoA_trans_6_Glutaconate, CoA_transf_3,
                     ECH_1, ECH_2, ETF, ETF_alpha, FGGY_C, HCDH, HCDH_N,
                     Hacid_dh, Hacid_dh_C, Mal_decarbox_Al, Peptidase_M22,
                     Peptidase_M23, Thiolase_C, Thiolase_N, ketoacyl-synt]) and
                     not (minimum(2, [AcetylCoA_hyd_C, BcrAD_BadFG, CoA_trans,
                     CoA_trans_230_acto, Mal_decarbox_Al]) and Lactoyl-CoA2 and
                     Lactoyl-CoA1) and not (minimum(4, [ACP_syn_III,
                     ACP_syn_III_C, Acyl-CoA_dh_1, Acyl-CoA_dh_2, AlaDh_PNT_C,
                     BcrAD_BadFG, ECH_1, ECH_2, ETF, ETF_alpha, HCDH, HCDH_N,
                     Hacid_dh, Hacid_dh_C, IlvN, NAD_Gly3P_dh_N, Thiolase_C,
                     Thiolase_N, ketoacyl-synt]) and HAAA1 and not HleuS1))"
                     /kind="single"
                     /product="Others_HGD_unassigned"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..4535
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Others_HGD_unassigned"
                     /region_number="1"
                     /rules="(minimum(1, [HAAA1, HG2B1, HG2B2, HGD-D, HleuD1,
                     HleuD2, HleuS1, HleuS2, Lactoyl-CoA1, Lactoyl-CoA2]) and
                     minimum(1, [ACP_syn_III, ACP_syn_III_C, AcetylCoA_hyd_C,
                     AcetylCoA_hydro, Acyl-CoA_dh_1, Acyl-CoA_dh_2,
                     AdoHcyase_NAD, AlaDh_PNT_C, BcrAD_BadFG, Biotin_lipoyl,
                     Carboxyl_trans, CoA_trans, CoA_trans_230_acto,
                     CoA_trans_6_Glutaconate, CoA_transf_3, ECH_1, ECH_2, ETF,
                     ETF_alpha, FGGY_C, HCDH, HCDH_N, Hacid_dh, Hacid_dh_C,
                     Mal_decarbox_Al, Peptidase_M22, Peptidase_M23, Thiolase_C,
                     Thiolase_N, ketoacyl-synt]) and not (minimum(2,
                     [AcetylCoA_hyd_C, BcrAD_BadFG, CoA_trans,
                     CoA_trans_230_acto, Mal_decarbox_Al]) and Lactoyl-CoA2 and
                     Lactoyl-CoA1) and not (minimum(4, [ACP_syn_III,
                     ACP_syn_III_C, Acyl-CoA_dh_1, Acyl-CoA_dh_2, AlaDh_PNT_C,
                     BcrAD_BadFG, ECH_1, ECH_2, ETF, ETF_alpha, HCDH, HCDH_N,
                     Hacid_dh, Hacid_dh_C, IlvN, NAD_Gly3P_dh_N, Thiolase_C,
                     Thiolase_N, ketoacyl-synt]) and HAAA1 and not HleuS1))"
                     /tool="gutsmash"
     CDS             108..4352
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Others_HGD_unassigned: HGD-D"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Others_HGD_unassigned: BcrAD_BadFG"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg89_1"
                     /sec_met_domain="HGD-D (E-value: 7.4e-65, bitscore: 205.8,
                     seeds: 370, tool: rule-based-clusters)"
                     /sec_met_domain="BcrAD_BadFG (E-value: 4.2e-39, bitscore:
                     120.7, seeds: 18, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNYKTLGIDIGSTTVKIAILDENENLIFADYKRHFANIQETLADL
                     LQEAFDQYGEMTLHPVITGSGGLTLANHLGVPFTQEVIAVSTSLQKLAPKTDVAIELGG
                     EDAKIIYFENGNVEQRMNGICAGGTGSFIDQMASLLQTDATGLNEYAKDYKALYPIAAR
                     CGVFAKTDIQPLINDGATKPDLSASIFQAVVNQTISGLACGKPIRGHVAFLGGPLHFLS
                     ELRESFIRTLKLDGEHTIIPENSHLFAAIGSALNAKEDNSISLTEMKDRLRNSIKLDFE
                     VERMEPLFASQEEYDAFAKRQSSYKVKTGDLATYKGNCYLGIDAGSTTTKTALVGEDGT
                     LLYSFYSSNNGNPLKTTIRSIKEIYELLPKDAKIAYSCSTGYGEALLKAALLLDEGEVE
                     TVSHYYAAAFFDPEVDCILDIGGQDMKCIKIRNKTVDSVQLNEACSSGCGSFIETFAKS
                     LNYTVQDFAKAAVFAQHPIDLGTRCTVFMNSKVKQAQKEGAEVSDISAGLAYSVIKNAL
                     YKVIKVSDASELGKHIVVQGGTFYNDAVLRSFEKIAGCEAIRPDIAGIMGAFGAALIAR
                     ERHQEGAETTMLSIDKINELKYTTSMANCRGCTNNCRLTINKFSGGRQYVSGNRCERGI
                     GKEKNKDHIPNLYEYKYKRIFSYTPLTADKATRGKVGIPRVLNMFENYPFWYTFFTELK
                     YEVVLSPTSTRKIYELGIESIPSESECYPAKLAHGHVTWLIRNGVKFIFYPCIPYERNE
                     FPDAVNHYNCPIVTSYAENIKNNVDELNDPSITFRNPFLAFTSEEILANRLVEEFKDIP
                     AEEVKAAVHKGWEEMAAARRDVQKKGEETLKYLEDTGRHGIVLAGRPYHIDPEIHHGIP
                     DLINSYGIAVLTEDSISHLAPVERPIRVNDQWMYHSRLYAAANYVKTRDDLDLIQLNSF
                     GCGLDAVTTDEVYEILDGSDKIYTCLKIDEVNNLGAARIRIRSLIAAIRAKKAQGQKRT
                     VKPASIDKVSFTKEMRKDYTILCPQMSPFHFSLLQAAFNSCGYNLEVLPNDNKHAVDVG
                     LKYVNNDACYPSLIVVGQIMDALLSGKYDLNKTAVVMSQTGGGCRASNYIAFIRRALKK
                     AGMEQIPVISVNLSGLESNPGFKLTLPLVKKVAYGAVFGDILMKCVYRMRPYELEEGIV
                     NRKHKIWEQRVISFLSGSSISHSQFKKMCREMVHEFDTIPISDVKKPRVGIVGEILVKF
                     LPAANNHLAELLESEGAEAVVPDLIDFMCYCFYNQNFKVENLGFKKSKATMANWGIKAI
                     EWVRKPASEALAQSRHFAPPADIRNLAKMASPIVSTGNQTGEGWFLTGEMMELIHGDVP
                     NIVCIQPFGCLPNHIVGKGVIKEIRREYPTANIVAIDYDPGASEVNQLNRIKLMLSTAQ
                     KNLKKAEEKMHK"
ORIGIN
        1 tttggcatat tcatgccaaa acacctcgcg ggatagtggt gtgtgaacag taacaggaaa
       61 atttcaattt tgtttatata caatacgagg aggcttgaat ttactttatg aattacaaaa
      121 cacttggaat tgacattggt tccactactg taaagatcgc gatcttagat gagaatgaaa
      181 atctcatttt cgcggattac aagcgtcatt ttgcgaacat tcaggaaacc cttgcagacc
      241 ttcttcagga ggcctttgac cagtatggcg agatgactct ccatcctgtg atcacaggtt
      301 ccggcggtct gacccttgcc aaccatctgg gcgttccttt tacccaggag gttattgcag
      361 tttctacttc tctgcagaag ctggcaccta agacagatgt tgccatcgag cttggcggtg
      421 aggatgccaa gatcatttat ttcgaaaacg gaaacgtaga acagcgtatg aacggtatct
      481 gtgcaggtgg caccggttct ttcatcgacc agatggcttc tcttctccag acagatgcca
      541 caggtctgaa tgaatatgcc aaagattaca aagcgctcta cccgattgct gcacgctgcg
      601 gtgttttcgc caaaacggat atccagccgc tgatcaatga cggtgcaacc aaaccagatc
      661 tgtcagcttc tattttccag gcagttgtaa accagaccat ctccggtctt gcctgcggaa
      721 aacccatccg tggacatgtt gcattcctgg gtggtccgct tcacttcctg tccgaactga
      781 gagaatcctt tatccgtact ttgaagctgg atggggaaca caccatcatt ccggagaact
      841 cccatctttt tgcagccatc ggttctgcat taaatgccaa agaagacaac tccatttccc
      901 tgacagaaat gaaagacaga ctccgcaatt ccattaagct ggattttgaa gtagaacgta
      961 tggaaccact tttcgcttct caggaagaat acgatgcatt tgcaaaacgt cagagttcct
     1021 acaaagtaaa aacaggagat ctggctacat ataaaggaaa ctgctatctg ggtattgatg
     1081 caggttccac cacaaccaaa actgcactgg taggcgaaga tggaacactt ctctattcct
     1141 tttacagtag caacaacgga aacccgttaa aaactaccat ccgttctatt aaagaaatct
     1201 atgaactgct tcccaaagac gcaaagatcg cttattcctg ttctacaggt tacggcgagg
     1261 ctctgctcaa agcggccctg ctcctggatg agggcgaggt tgagaccgta tcccattact
     1321 atgcagctgc attctttgac ccagaggtag actgtatcct ggatatcggc ggacaggata
     1381 tgaaatgtat caagatccgc aacaagaccg ttgacagcgt acagttaaat gaagcatgct
     1441 cctccggatg cggttccttt attgaaacct ttgccaaatc cctgaattat acagtacagg
     1501 atttcgcaaa agctgctgtt tttgcacagc atcctataga tcttggtacc agatgtactg
     1561 ttttcatgaa ttccaaggta aagcaggcgc agaaagaggg agctgaggtt tccgatattt
     1621 ccgcaggtct tgcttactct gttatcaaaa atgctctgta taaggttatc aaggtttctg
     1681 acgcttccga acttggcaag cacatcgttg tacagggtgg taccttctac aatgatgcgg
     1741 ttcttcgaag cttcgaaaag attgcaggct gcgaagccat ccgtccggat atcgccggaa
     1801 tcatgggtgc cttcggtgct gcactgattg cccgtgaacg ccatcaggaa ggcgcagaaa
     1861 cgactatgct ctccattgat aagatcaacg agctgaaata tacaacttct atggcaaact
     1921 gtcgtggatg taccaataac tgccgtctga ctatcaacaa attctccggc ggccgccagt
     1981 atgtaagcgg taaccgctgt gaacgcggta tcggtaagga aaagaacaaa gatcatattc
     2041 cgaatcttta tgaatacaaa tataaacgta ttttctccta cacaccgctt actgctgata
     2101 aggcaacgcg aggtaaagta ggtatcccgc gtgttctgaa tatgtttgag aattatcctt
     2161 tctggtatac tttctttaca gaactgaagt atgaggttgt gctctctcct acttccacca
     2221 gaaagattta tgagttgggt attgaatcca ttccaagtga atccgaatgc taccctgcca
     2281 agctcgcaca tggccatgta acctggctga tccgcaatgg tgtgaaattt attttctacc
     2341 cttgtattcc atatgaacgt aacgagttcc cggatgcagt caatcactat aactgcccga
     2401 tcgttacctc ttatgccgaa aatatcaaaa ataacgtaga cgaattaaat gatccgtcca
     2461 ttaccttccg caatccgttc cttgcattta cttctgagga aattctggca aatcgtctgg
     2521 tagaggaatt taaggatatc ccggcagaag aagtcaaagc tgctgtgcat aagggctggg
     2581 aggaaatggc tgctgcacgc cgcgatgtac agaaaaaagg ggaagaaacc ctgaaatatc
     2641 tggaagatac aggacgccat ggtattgtcc ttgcaggacg cccataccat attgacccgg
     2701 aaatccatca tggtatcccg gatctgatca acagctatgg tattgccgta cttaccgagg
     2761 attccatctc tcatctggca cctgtggaac gcccgatccg cgttaatgac cagtggatgt
     2821 accactccag actgtatgct gctgcaaact atgtaaagac acgcgatgac ctggatctga
     2881 tccagctcaa ctcttttgga tgtggtctgg atgcagttac tactgacgag gtttatgaaa
     2941 tccttgacgg aagtgacaaa atctatacct gcttaaagat tgacgaggta aataaccttg
     3001 gtgctgcaag aatccgtatc cgttccctga tcgcagcgat ccgtgcgaag aaggcacagg
     3061 gacagaaacg tactgtcaaa cctgcttcta ttgataaagt atcctttaca aaggaaatga
     3121 gaaaggacta taccatcctc tgcccgcaga tgtcaccgtt ccatttcagc ctcctgcagg
     3181 cagcattcaa ttcctgcgga tacaacctgg aggtgcttcc gaatgacaat aagcatgcag
     3241 tggatgttgg tctgaagtat gtaaacaatg acgcctgcta tccgtctctg atcgttgtgg
     3301 gacagatcat ggatgctctg ctttccggta aatatgacct gaacaaaacg gccgttgtca
     3361 tgtcacagac aggtggcgga tgtcgtgctt ccaactatat tgcatttata cgacgcgcat
     3421 taaagaaagc cggtatggaa cagattccgg ttatctctgt taacttaagc ggtctggaaa
     3481 gcaatcccgg attcaaactt acgcttccat tagtgaagaa agttgcttat ggtgccgtat
     3541 tcggagatat cctgatgaaa tgcgtatatc gtatgcgtcc ttatgaactg gaagagggaa
     3601 tcgtaaacag gaaacataaa atctgggaac agcgcgtgat ctctttctta agcggaagca
     3661 gtatcagcca cagccagttt aagaaaatgt gccgcgaaat ggttcacgaa tttgatacca
     3721 ttccgatcag tgatgtgaaa aagcctcgtg tcggcatcgt aggtgagatc cttgtcaagt
     3781 tcctgcctgc tgcaaataat catctggcag agcttctgga atctgaaggc gctgaggcag
     3841 ttgtaccgga tctgatcgat ttcatgtgct actgcttcta taatcagaac ttcaaggtag
     3901 aaaatcttgg attcaagaag tccaaggcaa ccatggcaaa ctggggaatc aaagcaatcg
     3961 aatgggtgag aaagcctgca agcgaagctc ttgcacagag ccgccatttt gcaccgcctg
     4021 ctgatatcag aaatctggca aaaatggcat ctccgatcgt ttccaccgga aaccagacag
     4081 gtgagggatg gttcctgaca ggtgagatga tggaactgat ccatggagat gttccgaata
     4141 ttgtttgtat ccagcctttc ggatgtctgc cgaaccacat tgtaggtaag ggtgttatca
     4201 aagagatccg cagagaatat ccgacagcca atattgtagc catcgactat gatccgggtg
     4261 caagtgaagt taaccagctc aaccgaatca aactgatgct ctccacagct cagaaaaacc
     4321 tgaaaaaagc agaagaaaaa atgcataaat aataccgtaa tggatatatt gcaatgtatt
     4381 ttaccttgca atatatccgc cttagagcgt atttgaaaaa tcattcccgc aatctacata
     4441 tcctgctttg cgaaaagcat ttttcacacg cgctcaacaa agagttatat ttcagaaatt
     4501 tttaatgtgt ttctatttca gaaatacatt taaac
//
