LOCUS       c00300_gnlPro..         6329 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|JGGBPCPD_300.
ACCESSION   c00300_gnlPro..
VERSION     c00300_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-05 18:07:35
            Original ID  :: gnl|Prokka|JGGBPCPD_300
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 6329
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..6329
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[1660:4637]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      1661..4637
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..6329
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..6329
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             256..885
                     /locus_tag="ctg300_1"
                     /transl_table=11
                     /translation="MITQYDLYETPSPKGEDGKKSLHARVCPKKTYTSQEFVEHVAAFE
                     HLPKNQIGGALAAIVDDLCYLLADGNIVELGDLGFFSTSLKCLKDTDDDEDEIKPKSIA
                     FQNVHLRISSEFRKRIKHEMKFQRVHSLTRRQKIVNSTVKERMKKMVSFLKINICITRK
                     EYIGLVGLTPYEAMNELNDFISQGLLRRRGAGRSVVYVAGENLKVE"
     CDS             1661..3901
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate2"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg300_2"
                     /sec_met_domain="Gly_radical (E-value: 1.3e-45, bitscore:
                     142.8, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate2 (E-value: 4.2e-285, bitscore:
                     935.1, seeds: 33, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKFENENWAQFKGEIWKREINVRDFIQNNYKPYEGDDSFLKDSSD
                     KTKKVWDKLTEMFKVEREKGVYDAETKYPQGIDTYGPGYIDKENEVIVGLQTDAPLKRG
                     IYPKCGTRMVENSLEAYGYKLDPMTKEIFTKYRKTHNEGVFSAYTDEMLAARRSAIITG
                     LPDAYGRGRIIGDYRRVALYGTEILIAEKKHYLKRLDIQELTEEIIQSREEISEQIKAL
                     KAFEKMCAGYGFDVTKPAKDAREAVQFVYLGYLAAVKDQDGAAMSLGRTSTFLDIYIEK
                     DIREGKLTEEEAQELIDQFIIKLRIVRFLRTPEYNDLFSGDPVWVTESLGGQGVDGRSL
                     VTKTSYRYLHTLYNLGPAPEPNLTVLWFNNAPENWKRFCAKVSIDTSAIQYENDDLMRP
                     DYGDDYGIACCVSPMKIGKQMQYFGARANLAKCLLYAINGGRDERSGVQVAPRFEPITS
                     EYLDYNEVMEKYEQMMRWLAKVYVNALKIIHYMHDKYAYEAFEMALHDGSVERIRATGI
                     AGLSIVADSLAAIRDTKVKVIRDERGLAVGFEREGDYVPFGNNDDRTDSIAVDITEKFM
                     EYLRQHQTYRNATPTQSILTITSNVVYGKKTGATPDGREGGTPFAPGANPMNGRDTKGA
                     VAALASVAKLPFQHAHDGISYTFAVSPATLGKERDIQIANMVGLLDGYFTPDGGQHLNV
                     NVFDKELLLDAMEHPENYPQLTIRVSGYAVNFVKLTREQQLDVISRTINHSL"
     CDS             3909..4637
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 69.3;
                     E-value: 1.3e-21)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg300_3"
                     /sec_met_domain="Radical_SAM (E-value: 9e-36, bitscore:
                     112.2, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="LEIKGNIHSLESFGTVDGPGIRFVIFMQGCPLRCLYCHNPDTWDS
                     KGLAKYQMTPEELLAEVLRYKNFIAKGGVTVTGGEPLLQAEFLREFFCLCREAGIHTAL
                     DTSGYICTPKALAVLEYADLVLLDIKTIDPELHPRLTAVKLDNTLRFLDELEKRGVPVW
                     IRHVIVPGLTDNDEALDKLAEYISHYTVVQKAELLPYHTMGAYKYEAQGLDYKLKEVEP
                     LSAERLANAKEIFKKHGITI"
     CDS             4726..5628
                     /locus_tag="ctg300_4"
                     /transl_table=11
                     /translation="MEQHHHDHHHHEITSLNKAFIIGISLNLAFVVAEFTAGFWYNSLG
                     LLSDAGHNLGDVASLVLAMLAFRLAKVHPTCSYTYGYKKSTVLVSLLNAVILLVAVGII
                     IAESIGKLLHPRPVEGDAIAWVAGIGVVINALTAWFFMKDKDKDLNVKGAYLHMAADAL
                     VSVGVVVSGIVIAHTGWYIIDPIIGLLIAIVIIVSTWGLLRDSIRLSLDGVPAGTDIDK
                     VEKTIHSVEGVEGVHHLHIWAISTTETALTAHVSVADISEMERIKQEIKHDLQHVGIQH
                     ATLEFETSGFKCDGHCCSD"
     CDS             5789..6328
                     /locus_tag="ctg300_5"
                     /transl_table=11
                     /translation="MEPIINSQAPEFKVQAYHNGSFKTVSSDDIKGKWAIFFFYPADFT
                     FVCPTELVDMADKYDQFQAMGVEVYSVSTDSHFVHKAWHDASETIRKIKYPMLADPTGV
                     LSRAFGVMIEEDGMAYRGTFLVNPEGKIKLAEINDNGIGRNADELLRKVEAAQFIAEHP
                     SEVCPAKWKKGGETLKP"
ORIGIN
        1 tcaacctact ttatcgataa gacatttagt agtcaactaa aaacgttaac atacagttcg
       61 tttaaaataa aaaatcgttc gacaatcatt ctttatgcat ttaacagcta tcgaataatc
      121 attcgacaat tgtcgaacga ataaagaatg attgtcgaat gatttttttt ctagaagcga
      181 aatttatttt tagaagtatt tcgatttatc agggagaaac aagttatatt tgaacaatta
      241 atatcaatat aacctatgat cacacaatac gacctgtatg aaacaccttc acccaaaggg
      301 gaagacggta aaaaatcgct gcatgcccgt gtttgtccga aaaagactta caccagtcaa
      361 gagtttgtag aacatgtggc agcctttgaa catctgccga agaatcagat tggcggggca
      421 cttgctgcca ttgtcgacga cctttgttat ctgcttgccg atggaaacat tgtggaatta
      481 ggtgatcttg gatttttttc tacttcattg aagtgcctga aggatacgga tgacgatgag
      541 gatgaaatca aaccgaaatc gatcgctttt caaaatgtac atctgagaat cagcagtgaa
      601 ttccgaaaaa gaatcaaaca tgagatgaaa tttcaacgcg tgcattccct tacgcggaga
      661 caaaaaattg tcaattctac cgtgaaagag cggatgaaaa aaatggtctc tttcctcaaa
      721 ataaacattt gtattacccg aaaggaatat ataggactgg tcggattgac accttacgag
      781 gcgatgaatg aactgaacga ttttatcagc cagggtcttc ttcgccgtcg cggggcgggc
      841 aggtctgtgg tatatgttgc gggggaaaat ctgaaggtag aataattata taggtaatat
      901 tttaccctgt aatccctatt tatggttaga aatgagctcg ttttacatat atataggtat
      961 tgcctgtgcc tttccgtggt tttacctttg ccgtatcaat taagttttac tattagtata
     1021 aatcgtcctg accaaaatcc gatttgttga tttttatgta cacctattat ttaaggtaaa
     1081 tacccatgga agggagtttt tttattggtt gtccctttca tatataaaag ggagccatcc
     1141 tgtgaagaat ggctcttttt tatttaacaa agaaagcaat ttttgcaaat attgtttatt
     1201 ccaatgatca attttcccga ttataacaca gaataggtag taggaagctc tttttttact
     1261 atataatagg tattgtgtac tccccttgac ggctctatct ttgcaacata gaaaaaagta
     1321 acactattag tataatcgct atatcaaaac cttttgctaa ttatgtaagc atgtaagatg
     1381 attaaacgtg tgtcggaaat tttttattgg ttgtttccgg cataaaaaag ggactctcct
     1441 gcgaaggatg gttccttttt tatttttttg atattattta taatccttcc tgtttgatac
     1501 ctatatcagg cgatacgctg cactatatct accgttttag taactatttg tagggatttt
     1561 ggccgttggg aatacatgat gtttttgttg attgtttcgc atgccatata aataacgtac
     1621 ctttgcaccg aaattaaaac cgaagaaaac aaaataaggt atgaagtttg aaaacgagaa
     1681 ctgggcgcag ttcaaaggcg aaatttggaa aagagagatt aacgtaaggg attttatcca
     1741 gaacaactac aaaccttacg aaggggacga ctctttcttg aaagactcct cagataagac
     1801 gaagaaagtt tgggataaat tgaccgaaat gtttaaggtt gaaagggaga agggtgttta
     1861 cgacgctgaa acaaaatatc ctcaaggtat agatacttac ggaccaggtt atattgacaa
     1921 agaaaacgaa gtaattgtcg gcctgcaaac cgatgccccc ctgaaacgcg gtatttaccc
     1981 gaaatgtggt acccgcatgg tggaaaacag tctggaagca tatggctaca aactggaccc
     2041 gatgacaaaa gaaatcttca cgaaatacag aaagacgcat aacgaaggcg tattctccgc
     2101 ttatacagat gaaatgcttg cagcgcgccg ttccgctatc atcaccggcc tgccggatgc
     2161 ttacggacgc ggacgtatta tcggtgacta ccgccgtgta gcactttacg gtactgaaat
     2221 cctgatcgct gaaaagaaac attatctgaa aagactcgat attcaggaac tgaccgaaga
     2281 gatcatccag agccgtgagg aaatatccga acagatcaag gcgctgaagg ctttcgaaaa
     2341 gatgtgtgcc ggttatggtt tcgacgtgac caagcctgca aaggatgcac gcgaagcggt
     2401 tcagtttgtc tatctgggtt atttggctgc ggtgaaggac caggatgggg ctgccatgtc
     2461 gttgggacgt acttctactt tcctcgatat ttatattgaa aaagatatcc gcgaaggcaa
     2521 gctgacggaa gaagaagctc aggagctgat cgaccagttt attatcaaac tgcgtatcgt
     2581 ccgtttcctt cgtactccgg aatataacga cctgttctcc ggtgaccctg tttgggtaac
     2641 cgagtctttg ggcggacagg gtgtcgacgg ccgttcattg gtgacgaaga cgtcttaccg
     2701 ttatctgcat acattatata acctcggccc ggcaccggaa cccaacttaa cggtactgtg
     2761 gttcaacaac gctcctgaaa actggaaacg tttctgtgcg aaggtttcta tcgatacttc
     2821 cgctatccag tatgagaacg acgatctgat gcgtccggat tatggagatg attatggtat
     2881 tgcctgctgc gtgtctccga tgaagatcgg taagcagatg cagtatttcg gggcacgtgc
     2941 caacctggct aaatgtttgt tgtatgccat caacggaggg cgcgatgaaa gatcaggtgt
     3001 acaggtggct ccgaggtttg aaccgatcac cagcgaatac ctggattaca acgaagtgat
     3061 ggagaaatac gaacagatga tgcgctggct ggctaaagtc tatgtgaatg cactgaagat
     3121 catccattac atgcacgata aatatgcgta tgaagcattc gagatggctt tgcatgacgg
     3181 ttcggtagaa cgtatccgcg caacaggtat tgccggcctt tctatcgttg ccgactcatt
     3241 ggctgctatc cgcgatacga aagtaaaagt gatccgcgac gaacgtggcc tggctgtcgg
     3301 tttcgaacgt gaaggagatt atgttccttt cggtaacaat gacgatcgta cagatagtat
     3361 tgctgtcgac atcactgaaa agttcatgga atatctgcgt cagcaccaga cataccgcaa
     3421 cgccactccg acacagtcta tcctgactat cacatccaat gtggtgtatg gaaagaagac
     3481 aggtgcaact cccgacggtc gcgaaggtgg tacgccgttt gctccgggtg cgaacccgat
     3541 gaacggacgt gataccaaag gcgctgttgc tgcactggct tctgtcgcta agctgccgtt
     3601 ccagcatgca cacgacggta tctcttatac attcgctgtt tctcctgcaa cgttaggaaa
     3661 agaacgtgat attcagatcg ccaatatggt cggcctgctg gacggttact ttactcctga
     3721 cggcggacag catctgaatg tgaacgtatt cgacaaggag ctgttgctcg atgctatgga
     3781 gcatccggaa aactatccgc agttgacaat ccgcgtatcg ggttatgcag taaactttgt
     3841 gaagctgaca cgcgaacagc agctggatgt gatttcaaga acaatcaatc attctttgta
     3901 attgaaagtt ggaaattaaa ggaaacatac attcactaga gagtttcggg accgttgatg
     3961 gtcccggaat tcgttttgtt atctttatgc aggggtgtcc gctccgttgc ctgtattgcc
     4021 acaatccgga tacctgggat agcaaagggc ttgccaaata tcagatgacg cctgaagaat
     4081 tattggctga ggttttacga tataagaact ttatcgccaa gggtggcgtg actgtcaccg
     4141 ggggagaacc cttattgcaa gctgaatttc tcagggagtt tttctgtctt tgccgggaag
     4201 ccggtattca tacggcgctc gatacatccg gctatatctg cacaccgaaa gccctggcgg
     4261 ttctggaata tgccgacctg gtcttattgg atataaaaac catcgatccc gaactgcatc
     4321 cccgtctaac ggccgtgaaa ctggataata cgttacgttt cctggacgaa ctggaaaaac
     4381 gcggtgttcc ggtttggata cgccatgtca ttgtgcccgg actgacggat aatgacgagg
     4441 ccttggataa actggccgaa tacatcagcc actatacggt tgtacagaag gcggaactct
     4501 tgccttatca cacgatgggc gcttataaat acgaggcaca aggtctcgac tacaaactga
     4561 aagaggtaga acctttatcg gccgaacgtc tggcaaatgc caaagaaata tttaagaagc
     4621 acggtataac catctgataa tttacctata atcaggtata tttttattgc tctaaaaaac
     4681 atagttttgt cccatattgt tactcaaata aaagtaggaa tgaatatgga acaacatcat
     4741 catgatcatc accaccacga aataacctct ttgaacaagg ctttcattat aggtatttcc
     4801 ctgaacctgg cttttgtcgt agccgaattt acggctggct tctggtataa ttcattagga
     4861 cttctgtcgg acgcagggca taacctggga gatgtggcca gcctggtact ggctatgctg
     4921 gctttccggc tggctaaggt gcacccgact tgcagctata cctatggata taagaaaagc
     4981 actgtactcg tatcccttct gaatgcggtc atcctcttgg tggccgtcgg gattattatt
     5041 gctgaaagta taggaaaact gctgcatccg cgtccggtcg agggtgatgc gatcgcttgg
     5101 gtggcaggta tcggagtcgt tataaatgca cttacggcat ggttttttat gaaggataag
     5161 gataaagact tgaatgtgaa aggtgcttac ttacatatgg ctgccgatgc actggtatcg
     5221 gtcggcgtgg ttgtctccgg aattgttatt gcccataccg gatggtatat catcgacccg
     5281 attatcggcc tgttgattgc gatcgttatc attgtttcta cctggggact gctgcgcgac
     5341 agtatccgtc tctctctgga cggtgtgccg gcgggaacgg acatcgataa ggtggaaaag
     5401 acgatacatt cggtcgaagg cgttgaaggc gtccatcatc tccatatctg ggcgatcagt
     5461 acgaccgaaa cggcacttac cgcccatgtt tccgttgcgg atatttcaga aatggaacgt
     5521 atcaaacagg agataaaaca cgaccttcag catgtgggaa tccagcatgc tacgctcgaa
     5581 tttgaaacgt cgggattcaa gtgcgatggg cactgttgct cagactgaaa caggtatatt
     5641 ataaacctga tttactgata ctcaaagatt ttgtctatga ctgcataaaa cctttcgatt
     5701 ggattttttg ttcttacctc gaatcggcaa cttatctttg caccagtaac aaaaacaaaa
     5761 tagtaataac gattaaaata aaaattctat ggaacctatt atcaattcac aagcacccga
     5821 gtttaaagtt caggcatacc acaacggtag tttcaaaact gtaagcagcg acgacatcaa
     5881 aggcaaatgg gcgattttct ttttctaccc ggctgacttt acatttgttt gtccgaccga
     5941 gttggtagat atggctgaca aatatgacca gttccaggca atgggtgtag aagtttactc
     6001 tgtaagtaca gattcacact tcgtacacaa agcatggcac gatgcttctg aaacgattcg
     6061 taagatcaaa tacccgatgt tggcagaccc gacaggcgta ttgagccgtg cattcggtgt
     6121 aatgatcgaa gaagacggta tggcttaccg cggtacattc ctggtaaatc cggaaggaaa
     6181 gattaagctg gcagaaatca acgataacgg tatcggccgt aacgccgacg aactgcttcg
     6241 taaagtagaa gctgcccagt tcatcgccga acatccgagc gaagtttgcc ccgccaaatg
     6301 gaaaaaaggt ggcgaaacat tgaaaccga
//
