LOCUS       c00160_gnlPro..         5630 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|NAKFNGPJ_160.
ACCESSION   c00160_gnlPro..
VERSION     c00160_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-05 17:49:47
            Original ID  :: gnl|Prokka|NAKFNGPJ_160
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 5630
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..5630
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[2:3053](-)"
                     /cutoff="10000"
                     /detection_rule="(minimum(1, [HAAA1, HG2B1, HG2B2, HGD-D,
                     HleuD1, HleuD2, HleuS1, HleuS2, Lactoyl-CoA1,
                     Lactoyl-CoA2]) and minimum(1, [ACP_syn_III, ACP_syn_III_C,
                     AcetylCoA_hyd_C, AcetylCoA_hydro, Acyl-CoA_dh_1,
                     Acyl-CoA_dh_2, AdoHcyase_NAD, AlaDh_PNT_C, BcrAD_BadFG,
                     Biotin_lipoyl, Carboxyl_trans, CoA_trans,
                     CoA_trans_230_acto, CoA_trans_6_Glutaconate, CoA_transf_3,
                     ECH_1, ECH_2, ETF, ETF_alpha, FGGY_C, HCDH, HCDH_N,
                     Hacid_dh, Hacid_dh_C, Mal_decarbox_Al, Peptidase_M22,
                     Peptidase_M23, Thiolase_C, Thiolase_N, ketoacyl-synt]) and
                     not (minimum(2, [AcetylCoA_hyd_C, BcrAD_BadFG, CoA_trans,
                     CoA_trans_230_acto, Mal_decarbox_Al]) and Lactoyl-CoA2 and
                     Lactoyl-CoA1) and not (minimum(4, [ACP_syn_III,
                     ACP_syn_III_C, Acyl-CoA_dh_1, Acyl-CoA_dh_2, AlaDh_PNT_C,
                     BcrAD_BadFG, ECH_1, ECH_2, ETF, ETF_alpha, HCDH, HCDH_N,
                     Hacid_dh, Hacid_dh_C, IlvN, NAD_Gly3P_dh_N, Thiolase_C,
                     Thiolase_N, ketoacyl-synt]) and HAAA1 and not HleuS1))"
                     /neighbourhood="10000"
                     /product="Others_HGD_unassigned"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      complement(3..3053)
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="10000"
                     /detection_rule="(minimum(1, [HAAA1, HG2B1, HG2B2, HGD-D,
                     HleuD1, HleuD2, HleuS1, HleuS2, Lactoyl-CoA1,
                     Lactoyl-CoA2]) and minimum(1, [ACP_syn_III, ACP_syn_III_C,
                     AcetylCoA_hyd_C, AcetylCoA_hydro, Acyl-CoA_dh_1,
                     Acyl-CoA_dh_2, AdoHcyase_NAD, AlaDh_PNT_C, BcrAD_BadFG,
                     Biotin_lipoyl, Carboxyl_trans, CoA_trans,
                     CoA_trans_230_acto, CoA_trans_6_Glutaconate, CoA_transf_3,
                     ECH_1, ECH_2, ETF, ETF_alpha, FGGY_C, HCDH, HCDH_N,
                     Hacid_dh, Hacid_dh_C, Mal_decarbox_Al, Peptidase_M22,
                     Peptidase_M23, Thiolase_C, Thiolase_N, ketoacyl-synt]) and
                     not (minimum(2, [AcetylCoA_hyd_C, BcrAD_BadFG, CoA_trans,
                     CoA_trans_230_acto, Mal_decarbox_Al]) and Lactoyl-CoA2 and
                     Lactoyl-CoA1) and not (minimum(4, [ACP_syn_III,
                     ACP_syn_III_C, Acyl-CoA_dh_1, Acyl-CoA_dh_2, AlaDh_PNT_C,
                     BcrAD_BadFG, ECH_1, ECH_2, ETF, ETF_alpha, HCDH, HCDH_N,
                     Hacid_dh, Hacid_dh_C, IlvN, NAD_Gly3P_dh_N, Thiolase_C,
                     Thiolase_N, ketoacyl-synt]) and HAAA1 and not HleuS1))"
                     /neighbourhood="10000"
                     /product="Others_HGD_unassigned"
                     /protocluster_number="1"
     cand_cluster    1..5630
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(minimum(1, [HAAA1, HG2B1, HG2B2, HGD-D,
                     HleuD1, HleuD2, HleuS1, HleuS2, Lactoyl-CoA1,
                     Lactoyl-CoA2]) and minimum(1, [ACP_syn_III, ACP_syn_III_C,
                     AcetylCoA_hyd_C, AcetylCoA_hydro, Acyl-CoA_dh_1,
                     Acyl-CoA_dh_2, AdoHcyase_NAD, AlaDh_PNT_C, BcrAD_BadFG,
                     Biotin_lipoyl, Carboxyl_trans, CoA_trans,
                     CoA_trans_230_acto, CoA_trans_6_Glutaconate, CoA_transf_3,
                     ECH_1, ECH_2, ETF, ETF_alpha, FGGY_C, HCDH, HCDH_N,
                     Hacid_dh, Hacid_dh_C, Mal_decarbox_Al, Peptidase_M22,
                     Peptidase_M23, Thiolase_C, Thiolase_N, ketoacyl-synt]) and
                     not (minimum(2, [AcetylCoA_hyd_C, BcrAD_BadFG, CoA_trans,
                     CoA_trans_230_acto, Mal_decarbox_Al]) and Lactoyl-CoA2 and
                     Lactoyl-CoA1) and not (minimum(4, [ACP_syn_III,
                     ACP_syn_III_C, Acyl-CoA_dh_1, Acyl-CoA_dh_2, AlaDh_PNT_C,
                     BcrAD_BadFG, ECH_1, ECH_2, ETF, ETF_alpha, HCDH, HCDH_N,
                     Hacid_dh, Hacid_dh_C, IlvN, NAD_Gly3P_dh_N, Thiolase_C,
                     Thiolase_N, ketoacyl-synt]) and HAAA1 and not HleuS1))"
                     /kind="single"
                     /product="Others_HGD_unassigned"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..5630
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Others_HGD_unassigned"
                     /region_number="1"
                     /rules="(minimum(1, [HAAA1, HG2B1, HG2B2, HGD-D, HleuD1,
                     HleuD2, HleuS1, HleuS2, Lactoyl-CoA1, Lactoyl-CoA2]) and
                     minimum(1, [ACP_syn_III, ACP_syn_III_C, AcetylCoA_hyd_C,
                     AcetylCoA_hydro, Acyl-CoA_dh_1, Acyl-CoA_dh_2,
                     AdoHcyase_NAD, AlaDh_PNT_C, BcrAD_BadFG, Biotin_lipoyl,
                     Carboxyl_trans, CoA_trans, CoA_trans_230_acto,
                     CoA_trans_6_Glutaconate, CoA_transf_3, ECH_1, ECH_2, ETF,
                     ETF_alpha, FGGY_C, HCDH, HCDH_N, Hacid_dh, Hacid_dh_C,
                     Mal_decarbox_Al, Peptidase_M22, Peptidase_M23, Thiolase_C,
                     Thiolase_N, ketoacyl-synt]) and not (minimum(2,
                     [AcetylCoA_hyd_C, BcrAD_BadFG, CoA_trans,
                     CoA_trans_230_acto, Mal_decarbox_Al]) and Lactoyl-CoA2 and
                     Lactoyl-CoA1) and not (minimum(4, [ACP_syn_III,
                     ACP_syn_III_C, Acyl-CoA_dh_1, Acyl-CoA_dh_2, AlaDh_PNT_C,
                     BcrAD_BadFG, ECH_1, ECH_2, ETF, ETF_alpha, HCDH, HCDH_N,
                     Hacid_dh, Hacid_dh_C, IlvN, NAD_Gly3P_dh_N, Thiolase_C,
                     Thiolase_N, ketoacyl-synt]) and HAAA1 and not HleuS1))"
                     /tool="gutsmash"
     CDS             complement(3..3053)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Others_HGD_unassigned: HGD-D"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Others_HGD_unassigned: BcrAD_BadFG"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg160_1"
                     /sec_met_domain="HGD-D (E-value: 6e-24, bitscore: 73.4,
                     seeds: 370, tool: rule-based-clusters)"
                     /sec_met_domain="BcrAD_BadFG (E-value: 6.2e-40, bitscore:
                     125.4, seeds: 18, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSADNKNTAVVYRLGIDIGSTTVKIAVLNEENEILFSDYERHFAD
                     IQNTLAGLLDKAYKQLGEFSVLPVITGSGGLTLAKHLEVPFVQEVIAVSSSLQELAPKT
                     DVAIELGGEDAKIIYFEGGNIEQRMNGICAGGTGSFIDQMASLLQTDAAGLNDYAKDYK
                     ALYPIAARCGVFAKTDIQPLINEGATKEDLSASIFQAVVNQTISGLACGKPIRGHVAFL
                     GGPLHFLSELKTAFIRTLKLDEEHTIVPDNSHLFAAIGSALNAKKDTPPVSVTAMRDRL
                     SANIHMEFEVNRMEPLFATKEDYDKFNERQSHYNVVTGDISSYSGNCYLGIDAGSTTTK
                     AALVGEDGSLLYSFYSNNNGNPLATSIRAIQEIYSHLPREAKIAYSCSTGYGEALIKAA
                     LLLDEGEVETVSHYYAAAFFDPEVDCILDIGGQDMKCIKIKNHTVDSVQLNEACSSGCG
                     SFIETFANSLNYSVQDFAKAALFAKNPIDLGPRCTVFMNSKVKQAQKEGAEVSDISAGL
                     AYSVIKNALYKVIKVSDASELGKHIVVQGGTFYNDAVLRSFERIANCEAIRPDIAGIMG
                     AFGAALIARERYTAGQETTMLPIEKINSLQYKTTMANCRGCTNNCRLTINKFSGGRQYI
                     SGNRCERGIGKEKNKDHIPNLFDYKYKMLFSYEPLTEDKAPRGKVGIPRVLNMFENYPF
                     WYTFFTRLGYEVVLSPTSTRKIYELGIESIPSESECYPAKLAHGHVSWLIKQGVKYIFY
                     PCIPYEREEFADSVNHYNCPIVTSYAENIKNNIDELNDPEIRFHNPFLAMTNEETITER
                     LLEEFKDIPSTEISDAAHAAWKEMAKCREDIRRKGEETLRYLDKTGRRGIVLAGRPYHI
                     DPEIHHGIPDLINSYGIAVLTEDSVSHLAPIERPLRVNDQWMYHTRLYAAANFVKEKDN
                     LNLIQLNSFGCGLDAVTTDQVNEILADSGKIYTCLKIDEVNNLGAARIRVRSLIAALRV
                     KEEQHIKRKISPSSIKKVVFTPEMRKNY"
     CDS             3335..4471
                     /locus_tag="ctg160_2"
                     /transl_table=11
                     /translation="MYKILKQEGMAKRAHFETVHGVVETPVFMNVGTVAAIKGAVATTD
                     LHEIGTQIELSNTYHLHVRPGDQVIKKLGGLHKFMNWDRPILTDSGGFQVFSLSKLRKI
                     KEEGVYFNSHIDGHKIFMGPEESMQIQSNLASTIAMAFDECPSSVADRKYVQDSVDRTT
                     RWLARCKEEMARLNAKEDTINPQQMLFGINQGAVYDDIRITHAKEIAKMDLDGYAVGGL
                     AVGESHEEMYRILDSVVPYLPADKPTYLMGVGTPANILEGVSRGVDFFDCVYPSRNGRH
                     GHVYTNFGKLNLFNAKYELDSRPIEEGCQCPACRSYSRAYIRHLLKAKEMLGMRLCVLH
                     NLYFYNTMMTEIRQALDEGRYEDYKQQKLERMAAGPKA"
     CDS             4589..4912
                     /locus_tag="ctg160_3"
                     /transl_table=11
                     /translation="MFLGQASAGGAGAWATMLLPLVVFFLLMYFMIMRPQKKEQQRVKS
                     MLSSMEVGDSVVTTSGFYGILIDITDEDVIVEFGNNRNCRIPMRKAAIAEVEKAGDGIA
                     EKD"
     CDS             5017..5628
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) SDH_beta"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg160_4"
                     /sec_met_domain="SDH_beta (E-value: 1.8e-16, bitscore:
                     49.0, seeds: 368, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAFISLFDVIGPNMVGPSSSHTAGAASMALLARRLFPGKIKKVRF
                     TLYGSFAQTYRGHGTDRALLGGIMGFQTDDLRIRDSLTLAREQGMEYEFILSDKLGDEH
                     PNTADMDIEGTCGEHLFVRGVSLGGGKVKIVRLNQIEVDFTGEYSTLVVSQTDKPGVVA
                     HITKVLSEKNVNIAFMRLYREERGARAYTIVESDEKIPEDV"
ORIGIN
        1 gtatagtttt tcctcatttc aggtgtaaat actacctttt tgatggatga cggcgatatt
       61 ttccgcttga tatgctgctc ttccttgaca cggagggcag cgatcaggga gcggacacgg
      121 atacgggcag cacccaggtt gttcacctca tcaattttca ggcaggtgta gatcttgccg
      181 ctgtcagcca gaatttcatt tacctggtca gtggtcacgg catccaggcc gcagccaaag
      241 gagtttaact ggatcagatt cagattatct ttttccttca caaagttggc agccgcatag
      301 agcctggtgt gatacatcca ttggtcattg acgcgcagag gccgttcaat aggagccaga
      361 tgagagacgg aatcctcagt cagcacggca atgccgtagg aattgatcaa atccggaata
      421 ccgtgatgaa tttccggatc aatgtggtaa ggtcttcctg ccagcacaat accccgccgt
      481 cctgttttat ccagatagcg cagggtttcc tcgccttttc tgcggatatc ctcccggcat
      541 ttggccattt ctttccaggc agcatgggca gcatcagaga tttccgtgga ggggatatcc
      601 ttaaattctt ccaacagacg ctctgtgatg gtctcttcat tggtcatggc taaaaatggg
      661 ttgtggaatc tgatctccgg gtcattcagc tcatcaatgt tgttctttat gttctccgca
      721 taggaggtga caattgggca gttgtagtgg ttaacagagt ccgcaaactc ttcccgctcg
      781 taggggatgc agggatagaa gatatatttg acaccctgtt tgatcagcca tgaaacgtgt
      841 ccgtgggcca gtttggccgg atagcactcc gattcactgg gaatggattc aatgcccagt
      901 tcataaatct ttctcgtgga ggtgggagag agcaccacct cataccccag ccgggtaaag
      961 aaggtatacc agaaaggata attctcaaac atgttcagaa ccctgggtat tcccaccttt
     1021 cctctggggg ctttatcctc tgtcagcggt tcgtaggaga acagcatttt gtatttataa
     1081 tcaaacagat tcgggatatg gtccttattc ttctccttgc cgatgccgcg ctcacagcgg
     1141 ttgccggaga tgtactggcg gcctcctgaa aatttgttga tggtcagccg gcagttgttg
     1201 gtacagcccc tgcagttagc catggtggtt ttatactgaa ggctgttgat cttctcaatg
     1261 ggcagcatgg tggtttcctg tcccgctgta tagcgctccc tggcgatcag ggcagcaccg
     1321 aaagcaccca tgatcccggc aatgtcgggt cttatggcct cacagttggc aatgcgctca
     1381 aagcttctta aaaccgcatc gttgtagaag gttcccccct gtaccacgat atgttttccc
     1441 aactccgagg catcggatac cttgattacc ttatacagag cgtttttaat aacagagtag
     1501 gccagacctg cggagatatc cgatacctca gcgccttcct tctgggcctg ttttactttg
     1561 gagttcatga ataccgtaca tctgggtccg aggtcaatgg gattcttggc aaaaagtgcg
     1621 gcttttgcaa aatcctgtac gctgtagttc agggaatttg caaatgtttc gataaaagaa
     1681 ccgcagccgg atgagcaggc ttcattgagc tgtacgctgt ctactgtatg gttttttatt
     1741 tttatgcatt tcatatcctg accgccgata tccaggatac agtctacctc aggatcaaag
     1801 aaggccgctg cgtagtagtg ggatactgtc tccacttccc cttcatccag aaggagagca
     1861 gcttttatca atgcctcccc gtatccggtg gagcaggagt acgcaatttt cgcctcacgg
     1921 ggcagatggc tgtagatttc ctgaatggca cggatactgg tagccagggg attgccgtta
     1981 ttgttgctgt aaaaggaata gagcagggag ccgtcttccc ccaccagtgc agcttttgtt
     2041 gttgtggagc cggcgtcaat gcccagatag cagtttccgc tgtaactgga aatgtcaccg
     2101 gttactacat tgtaatggct ctgccgctca ttgaatttat cataatcttc cttagtggcg
     2161 aacagaggct ccatacggtt tacttcaaat tccatatgta tgttggctga cagcctgtca
     2221 cgcatggcag tgacgctgac aggaggtgtg tcctttttcg cgttcagcgc agagccgatg
     2281 gcggcgaaca ggtgggaatt gtcaggaaca atggtgtgtt cctcatccag cttcagtgtg
     2341 cggatgaacg ccgtcttgag ttcggataaa aagtgaagcg gacctcccag aaatgccaca
     2401 tgtccgcgga tcggttttcc gcaggcaaga ccgctgatgg tttggtttac aacagcctgg
     2461 aatatggagg ctgaaaggtc ttcttttgta gcgccctcat tgatcagcgg ttgaatatct
     2521 gtcttggcaa atacaccgca cctggcagca atgggataga gtgctttgta atctttggcg
     2581 taatcattca gcccggcggc atctgtctgc agcagggagg ccatctggtc gatgaaagag
     2641 ccggttcctc cggcacagat accgttcata cgctgttcaa tatttccgcc ctcaaaataa
     2701 atgatctttg catcctcacc acccagttca atggccacat cggttttggg tgccagttcc
     2761 tgtaaggagg aggatacagc aataacctcc tgtacaaagg gaacctccag atgctttgcg
     2821 agtgtcagtc cgcctgaacc ggtgatcacc gggaggacgg aaaattcccc caattgcttg
     2881 tatgccttgt caagcagtcc ggccagcgta ttctggatgt ctgcaaagtg gcgttcataa
     2941 tcggagaaaa gaatttcgtt ttcttcattt aaaactgcaa tttttaccgt ggtggagcca
     3001 atatcaatgc caagacggta tacgactgca gtatttttat tatctgctga catagtcagg
     3061 agacctcctt cataaaaaat ggacggtttt tcgccgtcat atatgcattt cttcttattt
     3121 atatagtgcg attaaaaatc atttaacatt atacgacaat atttctaaaa atacaattgc
     3181 taatttgaaa ttggattatt ttagttatat atcctcagaa aaagaaaata tttcagccgg
     3241 ttgcgtttac atttcccata tggtagaata agccagacac aagtgccgga aacagccggt
     3301 aaaagaatta catttagaaa gaggaaacgg ttatatgtat aagatattaa aacaggaagg
     3361 tatggcaaag cgcgcccatt ttgaaactgt gcacggagtg gtggagacac ctgtctttat
     3421 gaacgtgggc acagtggcag ccatcaaagg ggccgtagcc acaacagacc tgcatgaaat
     3481 aggcacccag atagaattgt caaacacgta tcatcttcat gtgcgtcctg gcgaccaggt
     3541 gatcaaaaaa ctgggaggcc tccataagtt tatgaactgg gacagaccca ttctcacaga
     3601 ttccggcgga ttccaggtgt tttccctgtc aaagctcagg aagatcaaag aggaaggtgt
     3661 gtacttcaat tcccatattg acgggcataa aatttttatg ggaccggagg aaagtatgca
     3721 gatacagtcc aatctggctt ccaccattgc catggctttt gacgagtgtc cctcctcagt
     3781 ggcagacagg aaatacgttc aggattctgt ggacagaacg acaagatggc ttgcaagatg
     3841 taaggaagag atggcaagac tgaacgcaaa ggaggacacc atcaatcccc agcagatgtt
     3901 gtttgggatc aatcagggcg cagtatatga tgatatcagg atcacccacg caaaagagat
     3961 tgccaaaatg gaccttgacg gctatgcggt gggcggactt gcagtgggag agagccatga
     4021 ggagatgtac cggatcttgg acagtgttgt tccttatctt ccggcagaca agccaacgta
     4081 tctgatggga gtgggcacac cggccaatat tctggaggga gtgtccagag gcgtggattt
     4141 ctttgactgt gtatatccaa gcaggaacgg ccgtcacgga catgtgtata caaactttgg
     4201 aaaactgaat ctcttcaatg ccaaatatga gctggactcc cgccccattg aggaagggtg
     4261 tcagtgtccg gcctgcagaa gctacagcag ggcgtatatc cgccatcttc tgaaggcaaa
     4321 ggaaatgctg ggcatgcgtc tgtgcgtgct tcacaatctg tatttttaca ataccatgat
     4381 gacggaaatc cgccaggcac tggatgaagg ccgctatgaa gactataaac agcagaagct
     4441 ggagcgcatg gcagcaggcc ccaaggcata aaaataggaa aaacgtgtaa aatatataaa
     4501 aaaacctgaa aaacacttga agaaataaga aaaattgtgt attattgtaa taacgttcaa
     4561 aatagtataa tttgcaggag gaacccaaat gtttttagga caggcaagcg caggcggtgc
     4621 tggcgcatgg gcaaccatgt tgctgccgtt ggttgtattt tttcttttaa tgtatttcat
     4681 gatcatgaga ccgcagaaaa aggaacagca gagagtgaaa tccatgttat cttctatgga
     4741 agtgggagac agtgtggtaa ccacaagcgg tttttacgga atcttaattg atattacaga
     4801 tgaggatgtc atcgtagaat tcggtaacaa cagaaactgc agaattccca tgagaaaagc
     4861 ggctatcgcg gaagttgaga aagccggcga cggaatcgca gaaaaagatt gatcagaaga
     4921 ttgaaaagca aaacaggtgt tgcaggaaaa acagttctgc ggcgcctgtt ttttcgtctt
     4981 caaccatgca gattcagaaa atagagaggt gagaatatgg catttatcag tttatttgat
     5041 gtgatcggtc cgaatatggt gggtccttcc agctcccata cggccggcgc ggcatctatg
     5101 gcccttcttg cccggagact ttttccgggt aaaataaaaa aagtgcgttt taccctgtat
     5161 ggctcctttg cccagacata ccgggggcac ggcacagacc gggcgcttct aggcgggatc
     5221 atgggatttc agacagatga tctgcggatc agggattctc tgacactggc acgtgaacag
     5281 gggatggagt atgaatttat tctcagtgac aagcttggtg atgagcatcc aaacactgcg
     5341 gatatggata tagagggtac ctgcggggaa catctttttg tgcgcggcgt atcccttggc
     5401 gggggaaaag taaagattgt ccggctgaac cagatagaag tggattttac aggggagtac
     5461 agcactctgg tggtgagcca gacggacaag ccgggtgttg tggcacatat cacaaaggtt
     5521 ctcagcgaaa agaacgtgaa tattgcattt atgcgcctct atcgggagga gagaggtgcc
     5581 agggcatata ccattgtgga gtctgatgag aaaataccgg aagatgtgac
//
