LOCUS       c00008_gnlPro..        14450 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|OPOPPBOA_8.
ACCESSION   c00008_gnlPro..
VERSION     c00008_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-07 18:30:40
            Original ID  :: gnl|Prokka|OPOPPBOA_8
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 14450
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..14450
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[166:4450](-)"
                     /cutoff="10000"
                     /detection_rule="(minimum(1, [HAAA1, HG2B1, HG2B2, HGD-D,
                     HleuD1, HleuD2, HleuS1, HleuS2, Lactoyl-CoA1,
                     Lactoyl-CoA2]) and minimum(1, [ACP_syn_III, ACP_syn_III_C,
                     AcetylCoA_hyd_C, AcetylCoA_hydro, Acyl-CoA_dh_1,
                     Acyl-CoA_dh_2, AdoHcyase_NAD, AlaDh_PNT_C, BcrAD_BadFG,
                     Biotin_lipoyl, Carboxyl_trans, CoA_trans,
                     CoA_trans_230_acto, CoA_trans_6_Glutaconate, CoA_transf_3,
                     ECH_1, ECH_2, ETF, ETF_alpha, FGGY_C, HCDH, HCDH_N,
                     Hacid_dh, Hacid_dh_C, Mal_decarbox_Al, Peptidase_M22,
                     Peptidase_M23, Thiolase_C, Thiolase_N, ketoacyl-synt]) and
                     not (minimum(2, [AcetylCoA_hyd_C, BcrAD_BadFG, CoA_trans,
                     CoA_trans_230_acto, Mal_decarbox_Al]) and Lactoyl-CoA2 and
                     Lactoyl-CoA1) and not (minimum(4, [ACP_syn_III,
                     ACP_syn_III_C, Acyl-CoA_dh_1, Acyl-CoA_dh_2, AlaDh_PNT_C,
                     BcrAD_BadFG, ECH_1, ECH_2, ETF, ETF_alpha, HCDH, HCDH_N,
                     Hacid_dh, Hacid_dh_C, IlvN, NAD_Gly3P_dh_N, Thiolase_C,
                     Thiolase_N, ketoacyl-synt]) and HAAA1 and not HleuS1))"
                     /neighbourhood="10000"
                     /product="Others_HGD_unassigned"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      complement(167..4450)
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="10000"
                     /detection_rule="(minimum(1, [HAAA1, HG2B1, HG2B2, HGD-D,
                     HleuD1, HleuD2, HleuS1, HleuS2, Lactoyl-CoA1,
                     Lactoyl-CoA2]) and minimum(1, [ACP_syn_III, ACP_syn_III_C,
                     AcetylCoA_hyd_C, AcetylCoA_hydro, Acyl-CoA_dh_1,
                     Acyl-CoA_dh_2, AdoHcyase_NAD, AlaDh_PNT_C, BcrAD_BadFG,
                     Biotin_lipoyl, Carboxyl_trans, CoA_trans,
                     CoA_trans_230_acto, CoA_trans_6_Glutaconate, CoA_transf_3,
                     ECH_1, ECH_2, ETF, ETF_alpha, FGGY_C, HCDH, HCDH_N,
                     Hacid_dh, Hacid_dh_C, Mal_decarbox_Al, Peptidase_M22,
                     Peptidase_M23, Thiolase_C, Thiolase_N, ketoacyl-synt]) and
                     not (minimum(2, [AcetylCoA_hyd_C, BcrAD_BadFG, CoA_trans,
                     CoA_trans_230_acto, Mal_decarbox_Al]) and Lactoyl-CoA2 and
                     Lactoyl-CoA1) and not (minimum(4, [ACP_syn_III,
                     ACP_syn_III_C, Acyl-CoA_dh_1, Acyl-CoA_dh_2, AlaDh_PNT_C,
                     BcrAD_BadFG, ECH_1, ECH_2, ETF, ETF_alpha, HCDH, HCDH_N,
                     Hacid_dh, Hacid_dh_C, IlvN, NAD_Gly3P_dh_N, Thiolase_C,
                     Thiolase_N, ketoacyl-synt]) and HAAA1 and not HleuS1))"
                     /neighbourhood="10000"
                     /product="Others_HGD_unassigned"
                     /protocluster_number="1"
     cand_cluster    1..14450
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(minimum(1, [HAAA1, HG2B1, HG2B2, HGD-D,
                     HleuD1, HleuD2, HleuS1, HleuS2, Lactoyl-CoA1,
                     Lactoyl-CoA2]) and minimum(1, [ACP_syn_III, ACP_syn_III_C,
                     AcetylCoA_hyd_C, AcetylCoA_hydro, Acyl-CoA_dh_1,
                     Acyl-CoA_dh_2, AdoHcyase_NAD, AlaDh_PNT_C, BcrAD_BadFG,
                     Biotin_lipoyl, Carboxyl_trans, CoA_trans,
                     CoA_trans_230_acto, CoA_trans_6_Glutaconate, CoA_transf_3,
                     ECH_1, ECH_2, ETF, ETF_alpha, FGGY_C, HCDH, HCDH_N,
                     Hacid_dh, Hacid_dh_C, Mal_decarbox_Al, Peptidase_M22,
                     Peptidase_M23, Thiolase_C, Thiolase_N, ketoacyl-synt]) and
                     not (minimum(2, [AcetylCoA_hyd_C, BcrAD_BadFG, CoA_trans,
                     CoA_trans_230_acto, Mal_decarbox_Al]) and Lactoyl-CoA2 and
                     Lactoyl-CoA1) and not (minimum(4, [ACP_syn_III,
                     ACP_syn_III_C, Acyl-CoA_dh_1, Acyl-CoA_dh_2, AlaDh_PNT_C,
                     BcrAD_BadFG, ECH_1, ECH_2, ETF, ETF_alpha, HCDH, HCDH_N,
                     Hacid_dh, Hacid_dh_C, IlvN, NAD_Gly3P_dh_N, Thiolase_C,
                     Thiolase_N, ketoacyl-synt]) and HAAA1 and not HleuS1))"
                     /kind="single"
                     /product="Others_HGD_unassigned"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..14450
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Others_HGD_unassigned"
                     /region_number="1"
                     /rules="(minimum(1, [HAAA1, HG2B1, HG2B2, HGD-D, HleuD1,
                     HleuD2, HleuS1, HleuS2, Lactoyl-CoA1, Lactoyl-CoA2]) and
                     minimum(1, [ACP_syn_III, ACP_syn_III_C, AcetylCoA_hyd_C,
                     AcetylCoA_hydro, Acyl-CoA_dh_1, Acyl-CoA_dh_2,
                     AdoHcyase_NAD, AlaDh_PNT_C, BcrAD_BadFG, Biotin_lipoyl,
                     Carboxyl_trans, CoA_trans, CoA_trans_230_acto,
                     CoA_trans_6_Glutaconate, CoA_transf_3, ECH_1, ECH_2, ETF,
                     ETF_alpha, FGGY_C, HCDH, HCDH_N, Hacid_dh, Hacid_dh_C,
                     Mal_decarbox_Al, Peptidase_M22, Peptidase_M23, Thiolase_C,
                     Thiolase_N, ketoacyl-synt]) and not (minimum(2,
                     [AcetylCoA_hyd_C, BcrAD_BadFG, CoA_trans,
                     CoA_trans_230_acto, Mal_decarbox_Al]) and Lactoyl-CoA2 and
                     Lactoyl-CoA1) and not (minimum(4, [ACP_syn_III,
                     ACP_syn_III_C, Acyl-CoA_dh_1, Acyl-CoA_dh_2, AlaDh_PNT_C,
                     BcrAD_BadFG, ECH_1, ECH_2, ETF, ETF_alpha, HCDH, HCDH_N,
                     Hacid_dh, Hacid_dh_C, IlvN, NAD_Gly3P_dh_N, Thiolase_C,
                     Thiolase_N, ketoacyl-synt]) and HAAA1 and not HleuS1))"
                     /tool="gutsmash"
     CDS             complement(167..4450)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Others_HGD_unassigned: BcrAD_BadFG"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Others_HGD_unassigned: HGD-D"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg8_1"
                     /sec_met_domain="HGD-D (E-value: 1.1e-59, bitscore: 195.3,
                     seeds: 370, tool: rule-based-clusters)"
                     /sec_met_domain="BcrAD_BadFG (E-value: 1.6e-37, bitscore:
                     121.9, seeds: 18, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MENNTLHKLGIDIGSTTVKVAILDQEDHLLFSDYERHFANIQETL
                     SSLLQKAYDALGDFAVAPMITGSGGLTLAKHLGVPFVQEVIAVSTALQHYAPQTDVAIE
                     LGGEDAKIIYFEGGNVEQRMNGICAGGTGSFIDQMASLIQTDATGLNEYAKNYKAIYPI
                     AARCGVFAKTDIQPLINEGATREDLSASIFQAVVNQTISGLACGKPIRGHVAFLGGPLH
                     FLSELKAAFIRTLNLDDEHAITPENSHLFAAIGSALNYKPGETTTLVSLRDRLANGIKM
                     EFEVARMDPLFASQEEYDAFRARHDGHNVRSADLSTYEGNCYLGIDAGSTTTKIALVGE
                     DGSLLYSFYSNNNGSPLATAIRSIKEIYTLLPPKAKIVHSCSTGYGEALLKAALMLDDG
                     EVETVAHFYAAAFFDPEVDCILDIGGQDMKCIKIKNQTVDSVQLNEACSSGCGSFIETF
                     AKSLNYSVQDFAKEALFAKNPIDLGTRCTVFMNSKVKQAQKEGASVADISAGLAYSVIK
                     NALFKVIKLSDASQLGKNIVVQGGTFYNDAVLRSFEKIAGVECVRPDIAGIMGAFGAAL
                     IARERHEAGYQTSMLSIEQINALTFDTKLARCQGCTNHCLLTINRFSGNRQYITGNRCE
                     RGLGKEKNKDNIPNLYEYKNKRLFDYTPLSAEEAARGTVGIPRVLNMYENYPFWATFFK
                     KLGFSVVLSPQSTRKIYELGIDSIPSESECYPAKLAHGHVTWLIHQNVDFIFYPCIPYE
                     RNEFPDSNNHYNCPIVTSYAENIKNNVDEITSGQVKFLNPFMSFASEETLSSQLIKTFG
                     QSEFGIPESEIRDAVAEGWKELAVCRMEMQKKGEEVLQYLKETGRRGIVLAGRPYHVDP
                     EINHGIPELITSYGIAVLTEDSISHLQPVERPLIVMDQWMYHSRLYAAANYVKTRDDLD
                     LIQLNSFGCGLDAVTTDAVNDILTKSGKIYTCLKIDEVNNLGAARIRIRSLISAIRVRE
                     KKQTKRTIVPANYNRVVFTEEMRKNYTILCPQMSPIHFELLEPAFQTAGYNLVVPDVDS
                     RTCVDVGLKYVNNDACYPSLIVVGQLMAAVMSGGYDMSRTAVLISQTGGGCRASNYIGF
                     IRRALEKAGYPDVPVISINLSGLEKNPGFKLTLPLIQHGLYALEFGDIFMRCVYATRPY
                     EAVAGSTDELHEKWKKEVIAFITQKKMLSHGKFKKMCREIIRDFDNLPRKDIKKPRVGV
                     VGEILVKFHPAANNHLVELLESEGAEAVVPDLTDFLLYCFYNTGFKADNLGMSQKSKKI
                     GRLGINFFEWLRSAARDEFTKSRHFTAPAHIDDLARYARDIVSEGNQTGEGWFLTGEML
                     ELIHTGTPNIVCTQPFACLPNHVVGKGVIKELRHRYPGSNIVAIDYDPGASEVNQLNRI
                     KLMLSTANKNLAKQNAPEQKEQSAD"
     CDS             4712..5569
                     /locus_tag="ctg8_2"
                     /transl_table=11
                     /translation="MNWLNKLERKFGNWAIPNLIVWLIGAYTIGFVLYQVNPSIIGLLM
                     LSPYHILHGQVWRLITWVFMPTDTNLVYLLIMALFYYQLGTTLERTWGTFRFNVYIFGG
                     MLFTVIGAFLLYGIMYLLNGGVTTEMLLIGTSFSTSYINMSIFLAFACMYPDMQVLLMF
                     IIPIKMKWMAVVYGVLIALSFFETGWAGRVAIFMSLLNFIIFFFSTRNLKRYTPHEVHR
                     RQKFKADMRRPQGYAGGAKHKCAVCGRTELDDPTLEFRFCSKCEGNYEYCQDHLFTHQH
                     IRMS"
     CDS             complement(5645..6745)
                     /locus_tag="ctg8_3"
                     /transl_table=11
                     /translation="MAGSTLGTLFKITTWGESHGKGVGVVIDGCPAGLSLCEADIQKFL
                     DRRKPGQSRYATPRKEDDAVEILSGVFEGKTTGTPISLVVFNRNQQSKDYSEIASYYRP
                     GHADYTFDQKYGFRDYRGGGRSSGRETIGRVAAGAVAVKILNELGITFITYTKSIGPIA
                     VSSDRFNFSEVTKNPLYMPDAEAAENAENYLDACMAELNSSGGIVECIIKGMPAGIGDP
                     VFEKLNANLAKAVMSIGAVKGFEIGDGFDVAKATGKNNNDAFVLGEDGRIAKATNHAGG
                     ILGGMSDGSDIILRAAIKPTPSIAATQRTVNQSGEEISVNIKGRHDPIIVPRAVVVVES
                     MAAITIVDALFSNMSARMDALKDFYS"
     CDS             6870..8195
                     /locus_tag="ctg8_4"
                     /transl_table=11
                     /translation="MIEKYLVKKITEPVDWKVNVPGSKSMTNRALLMAVLSAGTVCVEG
                     VLFSDDSRHFLACLVALGFTVEIEEEKKCATVTGCGGRIPRGEAVIDVGSAGTAARFLA
                     AMLGFSDGCYEIRCSAQMKRRPMAELFDLLSGAGAKITFLEEEGHLPVRITGCRYAQAD
                     KTAGETPRELSLDITKSTQFLSALLLIAPMVPEGMKIHITSEKTDGSYIRITRRMMQEF
                     GVSVAFDGRDYEVPCRSSYRKETYMVEPDMSAACYFYAAAAVTGGRTLVRHVHMDNTQG
                     DLRFLEVLEHMGCTADDTPEGILVTGPADGALRGITVNMNDFSDQALTLAAIAPFADAP
                     VRIEGIGHIRGQECDRIHAIQTNLTALGIRCEEEETAATIYPGSPHGGSVETYDDHRVA
                     MAFSLVGLRTDGIVIENPACCRKTFENYFTVLSELTGKTVEG"
     CDS             8300..9451
                     /locus_tag="ctg8_5"
                     /transl_table=11
                     /translation="MYELLKQEGRAKRGVFHTVHGDIQTPVFMNVGTVAAIKGAVSTED
                     LEQIKCQVELSNTYHLHVRTGDKLIKEVGGLHKFMSWNRPILTDSGGFQVFSLAGLRKI
                     KEEGVYFHSHIDGAKIFMGPEESMQIQSNLGSTIAMAFDECPPALAERKYVEDSVARTT
                     RWLERCKNEMQRLNGLEDTVNRHQMLFGINQGAIYTDIRIDHAKRISELDLDGYAVGGL
                     AVGETHEEMYHVLDETVPYLPVNKPTYLMGVGTPANILEGVERGIDFFDCVYPSRNGRH
                     GHVYTNQGKINLFNQKYEKDMRPIEEGCGCPTCRRYSRAYIRHLLKAKEMLGMRLCVLH
                     NLYFYNTMMEEIRDALDAGNFASYKEEKLYGMGQINREKEMAK"
     CDS             9550..9867
                     /locus_tag="ctg8_6"
                     /transl_table=11
                     /translation="MFSILATESASSAGWISMVVWLVILFAFMYFFMVRPQKKEQQKKN
                     SMLAELAVGDTILTTSGFYGTVIDIADDTVIVEFGSNKNCRIPMQKAAISAVEKPEDVT
                     E"
     CDS             9956..12451
                     /locus_tag="ctg8_7"
                     /transl_table=11
                     /translation="MKSKKNTFVSRAAVGCVTTAIFLLLCIIKGIYPFGIATLDTVDFQ
                     SQWIPAYYHVWDVLHGDGSAFFDWRVGGGNNFAGVSAQFSLFSPFNLFFLFVKRSWIEK
                     SMIVFLLIKLLAMGYGMRWFLKEQILKEERYGIYILAGSVGYAVNGYTLLYYGMGWLDV
                     AAILPLLCYFFCRMAEREGDWRLGKNTLGYIVCLTLVFLINIPQAYMMCFFLIVFAGGY
                     FFLKRQDQPLKKNGILKFGLTSLLSLGIAAVIFLPAARAMLGSYRLSSEEYRGISGYFL
                     LLRQDGMDAVWKWVILGSVAVPLLFFVISGRRKREHIWQWYLVAAMVLPVFFEAVNLIW
                     HRGTYVCFPMRYGYMLVFAVICMAAARVPDFVGDSRVRRNILYAFLGIWCVQMVALGIS
                     LIQPETHTDETDFVGDATEIGQPLAGKTDVFHKAKLADGAIDNNYPLIAESTSYSNYLH
                     LMTSEQIRMNTLLGYSQVWTRLSDTGGTLASDIMLGYRYYVASKAGSWGKDTAADSLWP
                     LAETEKFTLWENAPVGDRAFFITKADYEAYAVTEAYTENVFENQNRLSELLFHTVPIDW
                     KKETYLDDQTGAELSYRFYADGKQILYLYGKDLQQAEITVNGERLYVPDYNDLYNESYP
                     ATGNQGILSLGCFSDEDITVEIRQSVGDSGAERAVFFGFLDPQELLEAVDAAGRAVTYE
                     LDRDSCKLTVESAGAEYLMLPVNTDSGWYCTVNGEERAISRLAGNLMLVPLDEGTNEIT
                     LCYVPRGMKKGAAVTGMALMLLAGWGVLSRLLRRKSVLDKIYAGLGCAAADIFVVVYAG
                     FLLVVYVIPVVYTIYLRWI"
     CDS             12461..13945
                     /locus_tag="ctg8_8"
                     /transl_table=11
                     /translation="MEKLQARKKVYLWLLILLSLCALIPLAVLSKYNHQSGDDYGYAYR
                     TFQIWRETHSFWNVIKEAFATSAEFYQKWQGLYASAVLLALEPGIFGDKYYALTGILLI
                     AIIVGANCAGTVYIFHRKCGCPRLEGFAAGCVMSFLMIEWMPSALQGLYWYNGAMNYVF
                     FHAVLLLLLCVLLSLAGEHPRRKAGRAVLAVLLAVLLAGGNHVTAFMGILLIVGFGIFA
                     AVKKRRQNLICCVITLAAMVAGFLFNVTSPGTRIRQSYFSDRPGVAGTIVTAVKTGIAC
                     INSWMGLAVIVTAVLLLPVLLPLVKKIRGQYGFSFRYPLLVFVLSVAWLCAMFCPPIYA
                     MGTPGDVRLTNVVYFSFVWLFVLNEAYLCGWAVTRLEASGAAALSGAAGEFPGSFLITA
                     AVLAVGMIIGCGENMTAWQAYLLIRSGEAQEYSAEADERYAVLCDSAGEDVVLKAYTAK
                     PWMLFLDDITEEKSDWRNEYMRVYFGLNSVVLEQTE"
ORIGIN
        1 ggatcatcga agatgcaaac atccggctgg ttagaaatgc cacatttttt gaaccaaatt
       61 tccctctgta tctccatctg aaatcccacg gtgtgatctt ctgataatat tacttatcac
      121 gaaaaggggc tgcacatttg atgcgcagcc cctttctctg tatctgtcag tcggcagact
      181 gttccttctg ctccggtgcg ttctgctttg ccagattttt gttcgcggtg gagagcatca
      241 gcttgatacg gttgagctgg tttacctcgc tggcacccgg atcgtaatcg atggccacga
      301 tattggaacc cggatagcgg tgacgcagtt ccttgatgac acccttgccg accacatggt
      361 tcggcaggca ggcgaacggc tgcgtgcaga cgatgttcgg tgttccggtg tggatcagct
      421 ctaacatctc accggttaag aaccagccct cgccggtctg gttgccctcg gatacaatgt
      481 ctcttgcata tctggcaaga tcatcgatgt gcgcaggtgc agtaaagtgc ctgctctttg
      541 tgaattcatc tcttgcagcg ctgcgcagcc actcaaagaa attgatgccc agacggccga
      601 tcttcttgga cttctggctc atgccgagat tgtctgcttt aaagcccgta ttgtagaagc
      661 agtagagcag gaaatcggtc agatccggta caacggcctc tgcaccctcg gattcgagaa
      721 gctccaccag atgattgttg gcagccggat ggaacttgac aagaatctcg ccgacaacgc
      781 cgacgcgcgg tttcttgatg tctttgcgcg gaaggttgtc aaaatcgcgg atgatctcgc
      841 ggcacatttt cttgaacttg ccgtgggaga gcatcttttt ctgggtgata aaggcgataa
      901 cctctttttt ccatttctcg tgcaactcgt ccgttgatcc ggcaaccgcc tcgtaagggc
      961 gcgttgcgta gacgcagcgc atgaagatat cgccaaactc cagcgcgtag agtccgtgct
     1021 ggatcagtgg cagtgtcagc ttaaagcccg ggttcttctc cagaccgctc agattgatcg
     1081 agatgaccgg aacgtccgga tagccggctt tttccagtgc acgccggatg aagccgatgt
     1141 aattgctggc gcggcagccg ccgccggtct gggagattaa gaccgcggtg cgcgacatat
     1201 cataaccgcc ggacatcacg gccgccatca gctggccaac cacgatcagg gacgggtagc
     1261 aggcgtcatt gttgacgtac tttaagccga catccacgca ggtgcggctg tccacatccg
     1321 gaacaacaag gttgtacccg gccgtctgga atgccggctc caacagctca aaatggatcg
     1381 gtgacatctg tgggcagagg atggtgtaat tcttgcgcat ttcctccgtg aagacgacgc
     1441 ggttgtaatt cgccggaacg atggtgcgct tcgtctgctt cttctcacgg acacggatcg
     1501 ccgagatcag ggaacggata cggatgcgcg ccgcaccgag gttgttgacc tcatcgattt
     1561 ttaaacaggt gtagatcttg ccggactttg tcagaatatc attgacagcg tctgtcgtga
     1621 cggcatccag accacagccg aaggaattca gctggatgag gtcgagatcg tctcttgtct
     1681 tgacatagtt cgccgctgcg tacagtctgg agtgatacat ccactgatcc atgacgatga
     1741 gtgggcgctc caccggctgt aagtgggaga tggaatcctc cgtgagcacg gcgatgccgt
     1801 aggaggtaat cagctccgga atgccgtggt tgatctcagg gtctacatgg tacggacggc
     1861 ctgcgaggac gataccgcgt cttccggtct cctttaaata ctgtaagact tcctcgcctt
     1921 ttttctgcat ctccatccgg cagacggcaa gctccttcca gccttcggca accgcgtcgc
     1981 ggatctcgga ttccggaatc ccgaactccg actggccaaa ggtcttaatc agctgggagg
     2041 aaagcgtctc ctcgctggcg aaagacataa acggatttaa gaacttcacc tgaccggaag
     2101 tgatctcgtc cacgttattt ttgatatttt ccgcgtagga ggtaacgatc ggacagttgt
     2161 agtgattgtt ggaatccggg aattcgttgc gttcataagg aatacacggg tagaaaataa
     2221 aatcgacgtt ctggtggatc agccatgtca catgaccgtg tgccagcttt gctgggtagc
     2281 actcggactc gctcgggatg gaatcaattc caagctcgta gatcttgcgc gtcgactgcg
     2341 gggagagtac cacggaaaaa ccgagcttct taaagaaagt tgcccagaac gggtagttct
     2401 cgtacatatt taaaacgcgc ggaataccga ctgtgccgcg tgctgcttcc tcggcggaaa
     2461 gcggggtgta gtcaaacagg cgcttgttct tgtattcgta gaggtttgga atgttgtctt
     2521 tatttttctc cttgccgagg ccgcgctcac agcggttgcc ggtgatatac tggcggttgc
     2581 cggaaaagcg gttgattgtg aggagacagt ggttggtaca tccctgacat cttgccagct
     2641 ttgtgtcgaa ggtcagcgca ttgatctgtt cgatggaaag catggaggtc tggtagcctg
     2701 cctcgtgacg ctctctggcg atcagtgcgg caccgaaagc acccatgatg ccggcaatat
     2761 ccggacggac acattcgaca cccgcgattt tctcaaaact gcgcagtacg gcatcgttgt
     2821 aaaacgtacc gccctgtacg acgatatttt tcccgagctg gcttgcatcg gaaagcttga
     2881 ttaccttaaa taaagcattc ttgatgacag agtacgcaag acccgcagaa atatctgcaa
     2941 cggatgcgcc ctctttctgc gcctgcttta ccttggaatt cataaatacg gtgcagcggg
     3001 ttccaagatc gatcggattt ttggcaaaaa gagcctcctt tgcaaaatcc tgtacagagt
     3061 agttgaggga ttttgcaaac gtctcgatga aagaaccgca gccggaggaa caagcctcat
     3121 tgagctgtac ggaatccacc gtctgattct tgatcttgat acatttcata tcctgaccgc
     3181 cgatgtctaa gatacagtcg acttccgggt cgaagaacgc ggccgcatag aaatgagcca
     3241 ccgtctcaac ctcgccgtca tcgagcatca aagcggcttt taacagcgct tcgccgtatc
     3301 cggtggagca ggagtggacg atctttgcct tcggtggaag gagcgtatag atctccttga
     3361 tggaacggat tgccgttgca agtggacttc cgttgttgtt gctgtagaaa gaatacaaaa
     3421 gggaaccgtc ctcgccgact aaggcaatct tggtcgtggt ggaacctgca tcgatgccca
     3481 gatagcagtt gccctcataa gtggagagat ctgcggacct gacattgtgt ccgtcgtggc
     3541 gcgcacggaa agcgtcgtac tcttcctggc tggcaaacag aggatccata cgggcaacct
     3601 caaattccat cttgatgccg ttcgcgagcc ggtcgcggag agagacgagc gtggtcgtct
     3661 cacccggctt gtaatttaaa gcggaaccga tcgcagcaaa tagatgggaa ttctccggtg
     3721 tgatggcgtg ttcgtcgtcc agattcaagg tgcggataaa agcagccttg agctcggaaa
     3781 ggaagtgaag cggacctccg agaaaggcaa catgaccgcg gatcggctta ccgcaggcaa
     3841 gaccgctgat cgtctgattg accactgcct ggaaaatgga ggcagagaga tcctctctgg
     3901 tagcgccctc gttgattaac ggctggatat cggtcttggc aaagacaccg caacgcgcag
     3961 cgatcgggta gatggccttg taattcttgg cgtattcatt cagacctgtc gcatccgtct
     4021 ggatgaggga tgccatctgg tcgataaaag aaccggtacc gccggcacag atgccgttca
     4081 tacgctgctc gacattgccg ccctcaaaat agatgatctt ggcatcctca ccaccgagct
     4141 cgatggcaac atcggtctgc ggtgcataat gctgcagggc agtggacacg gcgatcactt
     4201 cctggacaaa cggaacaccg agatgcttgg cgagtgtcag accgccggaa ccggtaatca
     4261 tcggtgcaac agcaaagtcg ccgagtgcat cgtaagcttt ctggagaagg gaggataagg
     4321 tctcctggat attggcaaaa tggcgctcat aatcagagaa gagcagatga tcctcctggt
     4381 ctaaaattgc gaccttgacg gttgtggaac cgatgtcgat tcccaattta tgaagtgtat
     4441 tgttttccat aattccctcc atggctgttt ctcgtaaagt aaatacttta gcagagtaaa
     4501 atatgtaaaa gatttcttct tattataaat agtgccaatc tggcgataaa cactctacat
     4561 tatacgacag gatatgtcaa aagacaatgt gcattttgcc ggaaaagtat aaactttttg
     4621 taaagaagaa tccctctgat tgacaaagat ttccccgacc gatatactga acaacgtaat
     4681 gtcatttttg gaaaggtcag gaacagagtg tatgaactgg ttaaataaat tagaacgtaa
     4741 attcggcaac tgggcgattc ccaatctgat cgtgtggctg atcggagctt acacgatcgg
     4801 atttgtcctc taccaggtga acccatcgat cataggactt ttgatgttga gcccgtatca
     4861 catcctgcac ggacaggtct ggcgcctgat tacctgggtg tttatgccca cagacacgaa
     4921 tctggtgtat cttttgatta tggcgctttt ctattatcag cttggaacga cactggagcg
     4981 cacatgggga accttccggt ttaacgtcta tattttcgga ggtatgctgt ttaccgtcat
     5041 cggagcgttt ctgctgtacg gtatcatgta tcttttgaac ggcggtgtga cgacggagat
     5101 gcttctgatt ggaacaagct tcagcacaag ctacatcaac atgtccatct tccttgcgtt
     5161 tgcatgtatg tatccggata tgcaggttct tctgatgttc attatcccga ttaagatgaa
     5221 gtggatggcg gttgtgtacg gcgttctgat tgctttaagc ttctttgaga ccggatgggc
     5281 gggaagagtc gcgattttta tgtcgctcct caactttatt attttcttct tctccaccag
     5341 aaacctcaag cgctatacgc cgcacgaggt gcacagacgc cagaagttta aggcagacat
     5401 gcgaagaccg cagggatatg cgggcggtgc gaagcacaag tgtgctgtct gcggcaggac
     5461 ggaacttgac gatccaacgc tggaattccg tttctgctcc aagtgcgagg gcaattacga
     5521 atactgtcag gatcatctgt ttacacatca gcatattcgg atgagctaga aagtatgaaa
     5581 aagaaaaagg tgatacttcg attccgccag tcggcggaga agaagtatca cctttttctt
     5641 tcttttagga gtagaaatct tttagcgcat ccatccgtgc actcatattg gaaaaaagtg
     5701 cgtcgacgat cgtgatggct gccatggatt ccaccacgac aacggcgcgc ggaacgatga
     5761 tcggatcgtg gcgtcccttg atgttaaccg agatctcctc cccggactga ttgacagtgc
     5821 gctgtgtggc ggcgatggaa ggggtcggct tgatggcagc ccgaaggatg atgtcgcttc
     5881 cgtcgctcat gccgcctaag ataccgccgg catggttggt tgctttggca atcctgccgt
     5941 cctcaccgag gacaaaggca tcgttgttgt tcttgccggt cgcttttgct acgtcaaatc
     6001 cgtccccgat ctcaaaacct ttgacggcac cgatggacat gaccgccttg gcaaggttcg
     6061 cattaagctt ctcgaagacc ggatctccga tgccggcggg catccccttt atgatacact
     6121 ccacaatgcc gccggaggaa ttcagttccg ccatgcaggc atccagatag ttttcggcgt
     6181 tctcggctgc ctcggcgtcc ggcatataga gcggattctt cgtgacttcg gaaaaattaa
     6241 aacgatcgga agatacggcg atcgggccga tggattttgt gtaggttata aaggtaatgc
     6301 cgagctcgtt tagaatcttg acggcgacgg cgccggcggc gacacgcccg atggtttccc
     6361 gcccggagga gcgcccgccc ccgcggtagt cgcggaagcc gtatttctgg tcgaacgtgt
     6421 aatcggcgtg accgggacgg taataggagg caatctcgct gtagtctttg gactgctggt
     6481 tgcggttgaa gacgacaagc gagatcgggg tgccggtcgt ctttccttca aacacgccag
     6541 agagaatctc tacggcgtcg tcctctttgc gcggcgttgc atatctggac tgaccgggct
     6601 tgcgccggtc gagaaacttc tggatatccg cctcacagag agagagtccc gccggacagc
     6661 cgtcgatgac gacgccgacg ccctttccgt gagattcccc ccaggttgtg attttaaaaa
     6721 gagttcccaa agtagaccct gccatgtgat tccctcctgc ttacataatg atctttctcg
     6781 attataaaag atttgacttt gcgccgcaac agaaatatgt tatgatttga cggagcagaa
     6841 gtaccagcag gcgaggagaa gaatatttca tgatagagaa ataccttgtg aaaaagataa
     6901 cagagccggt ggactggaag gtaaacgtgc cgggatcgaa gagcatgacg aaccgcgcgc
     6961 ttcttatggc agtgctgtcc gcagggacgg tgtgcgtgga aggagtcctg ttttccgatg
     7021 attccagaca ctttctggcg tgtcttgtgg cgctcggctt tacagtggag atagaggaag
     7081 aaaaaaagtg cgcgaccgtg acgggctgcg gcggcaggat tccccgcggg gaggcggtaa
     7141 tcgacgtcgg aagcgccggg acagcggcgc gttttcttgc ggcgatgctc ggattttcgg
     7201 acgggtgcta tgagatccgc tgctcagcgc agatgaagcg gcggccgatg gcggagctgt
     7261 tcgatctgct ttccggtgcg ggagcgaaga ttacattcct cgaggaggaa gggcatcttc
     7321 cggtgcgcat cacaggatgc agatacgccc aggcggataa gacggcaggg gagacgccgc
     7381 gggagctctc tcttgacatt acgaagagca cacagttttt aagcgcgctg ctgttgattg
     7441 cgccgatggt tccggaaggg atgaagatcc acatcaccag tgagaagacg gacggctcat
     7501 acatccgcat cacgcggagg atgatgcagg aatttggcgt gagtgtcgct tttgacggaa
     7561 gagactacga ggtgccgtgc agaagttcct accggaaaga gacttatatg gtggaaccgg
     7621 atatgtcggc tgcctgctat ttttatgcgg ctgcggctgt gaccggcgga cggacgcttg
     7681 tgcggcatgt gcatatggac aacacacagg gagatctgcg tttcctggaa gtgttggagc
     7741 acatgggatg taccgcggac gatacgccgg agggcatcct ggtaacggga ccggcggacg
     7801 gtgcgctacg gggcatcaca gtcaatatga acgatttttc cgatcaggcg ctgaccctgg
     7861 cggcgatcgc gccgtttgcc gacgcgccgg tgcgcataga gggaatcgga catatccgtg
     7921 ggcaggaatg cgaccggatt cacgcgatac agacgaacct gacagcgctt ggcatccggt
     7981 gtgaggagga agagacggct gccacgatct atcccggcag cccccacggc ggaagcgtgg
     8041 agacatacga cgatcaccgc gtggcgatgg cattttccct ggtcggactt agaaccgacg
     8101 ggattgtcat tgagaacccg gcgtgctgcc gcaagacgtt tgaaaattat tttaccgtgt
     8161 taagcgaact gaccgggaaa acggtggaag gctgacaaga tgccggactg cacaaggctg
     8221 gtggaagtga aagcggaaaa agcgcagaac cagagaaaat aaagaagaac tacaggcaat
     8281 aaaaggagac gaaaaacaga tgtatgaatt attaaagcag gaaggacgcg cgaagagagg
     8341 cgttttccat acggtgcacg gagatatcca gacgccggta tttatgaatg tgggtacggt
     8401 ggctgccatc aagggagctg tctccacgga agatttagag cagatcaaat gtcaggtgga
     8461 gctgtcaaac acctaccatc tgcatgtgcg caccggagac aagctgatca aggaagtggg
     8521 aggactgcac aagtttatgt cgtggaatcg tccgattctt acggattccg gcggatttca
     8581 ggtgttctcc ctggcaggcc tgcgcaagat caaggaggag ggcgtttatt tccactcgca
     8641 tattgacggg gcaaagattt tcatgggacc ggaggagagc atgcagatcc agtccaatct
     8701 cggctccacg atcgccatgg cgttcgatga atgtccgccg gcgcttgccg agcggaaata
     8761 tgtggaggat tccgtggcac ggacgacgcg ctggctggag cgctgcaaga acgagatgca
     8821 gcgcctcaac ggtctggagg ataccgtgaa ccgtcaccag atgctgttcg gcatcaacca
     8881 gggagccatc tacacggaca tccggatcga ccatgcaaag cggatttccg agctggatct
     8941 ggacggctat gcggtcggcg gacttgcggt cggcgagaca cacgaggaga tgtaccatgt
     9001 gctggatgag accgtgcctt atcttccggt gaacaagccg acctatctca tgggagtcgg
     9061 aacgccggcc aatattctgg agggagtgga gcgcggcatt gacttctttg actgtgtcta
     9121 tccgtcccgc aacggccgcc acggtcatgt atacacgaat cagggcaaga tcaacctgtt
     9181 caaccagaag tacgagaaag atatgcgccc gatcgaggag ggctgcgggt gcccgacctg
     9241 ccgccgctac agcagagcct atatccgcca tctgctcaag gcaaaagaga tgcttggcat
     9301 gcgcttatgc gttctgcaca atctgtactt ctacaacacg atgatggaag agatccggga
     9361 tgcgctggat gccggaaatt ttgcctccta caaggaagaa aagctatacg gcatgggaca
     9421 gatcaaccgg gaaaaggaaa tggcaaagta aaagggaaaa ggcttgccaa acggcgaggt
     9481 tttgtgtaca atactcttta atgaagtcgg atgacttcgg acaagtttat gattttagga
     9541 ggatatcaga tgttttcaat cttagcaaca gagagtgcgt catccgctgg ctggatcagt
     9601 atggtggtgt ggctggttat tttatttgcg tttatgtatt tcttcatggt gcgtccgcag
     9661 aagaaggaac agcagaagaa gaactccatg cttgcagaac ttgcagtggg agatacgatc
     9721 ttaacaacaa gcggttttta tggaacagtg atcgatattg ctgatgatac agtaatcgtt
     9781 gagttcggaa gcaacaagaa ctgccgcatc ccgatgcaga aggcggccat ctccgcagtt
     9841 gagaagccgg aagatgtaac tgaataagaa gatttttaag agggatagta tataaaatgt
     9901 attatccctt ttctttttga gagagtattt cggagagcga ggagagattg tccggatgaa
     9961 aagtaaaaaa aatacgtttg tttcacgtgc cgccgtcggc tgcgtgacaa ctgccatttt
    10021 tctgctgttg tgcatcataa agggcatcta tccgtttgga attgccacgc ttgacactgt
    10081 ggattttcag tcccagtgga ttccggcata ttaccatgta tgggacgtgc tgcacggaga
    10141 cggcagcgcc ttttttgact ggagagtcgg cggcgggaat aattttgcgg gagtgagcgc
    10201 gcagttttct ctgttcagcc cgtttaacct gttcttcctc tttgtgaaaa gaagctggat
    10261 tgaaaaatcc atgatcgtgt ttcttttaat caagctgctt gccatgggct atggcatgcg
    10321 ctggttttta aaagagcaga tccttaagga agagcgttac ggaatctaca ttctggcggg
    10381 aagcgtgggg tatgccgtaa acggctatac gcttttgtac tatggcatgg gctggctgga
    10441 tgtggcagcg atcctgccgc tattgtgtta tttcttctgc aggatggcgg agcgggaagg
    10501 ggactggcgt ctcgggaaaa atacgctcgg ctatatcgtc tgcctgacgc tggtattcct
    10561 catcaatatt ccgcaggcgt acatgatgtg ttttttcctg atcgtgtttg cgggaggata
    10621 ttttttcctg aaacgacagg accagccgct aaaaaagaac ggcattttaa aattcgggct
    10681 gacttcttta ctgtcgcttg gaattgcggc ggtaatattt ctgccggcag cgagggcaat
    10741 gcttggttcc taccggctgt cgagcgagga gtaccgggga atctccggct attttctgct
    10801 gcttcggcag gacggaatgg acgcggtgtg gaaatgggtg attcttggca gcgtggcggt
    10861 tccgctgctg ttcttcgtca tttctggcag acggaagcgg gagcacatct ggcagtggta
    10921 tctggtggcg gcgatggtgc tgccggtttt cttcgaggcg gtcaatctca tctggcaccg
    10981 cgggacgtat gtgtgcttcc cgatgcgata tggttatatg ctggtgtttg ccgtgatttg
    11041 tatggcagcg gcgcgcgtgc cggattttgt gggagatagc cgcgtgcggc ggaacatttt
    11101 atatgcgttc ctgggaatct ggtgcgtgca gatggtggcg cttggcattt ctttgataca
    11161 gccggaaacg cacacggacg agacggattt tgttggggat gccacggaaa tcggtcagcc
    11221 gcttgctgga aaaaccgatg ttttccacaa ggcaaagctg gcggacggag cgattgacaa
    11281 caactatccg ctgatcgcag agagcacctc ctactccaat tatctgcatc tgatgacgtc
    11341 ggagcagatc aggatgaaca cactgctcgg gtattctcag gtgtggacca gattaagtga
    11401 cacgggcgga acgctcgcgt ccgacatcat gctggggtac cgttattatg tggcgtctaa
    11461 ggcgggatcg tggggaaaag acacggcggc ggattccctt tggccgcttg ctgagaccga
    11521 aaagtttacg ctgtgggaaa atgcgcccgt cggggacagg gcatttttta tcacaaaagc
    11581 ggactatgaa gcgtatgcag tcacggaagc gtacacggaa aatgtgtttg agaaccagaa
    11641 ccggctgtcg gagctgcttt ttcacacagt tcccattgac tggaaaaaag agacctatct
    11701 ggatgatcag accggagcgg aattatcgta ccggttttac gcggatggaa agcagatctt
    11761 atatctctat ggaaaggatc tgcagcaggc agaaattacg gtaaacgggg agaggctcta
    11821 cgtcccggat tacaatgatc tgtacaatga gagctacccg gcaaccggca atcagggaat
    11881 cctctccctc gggtgttttt cggacgaaga catcacggtg gaaatccgtc agtctgttgg
    11941 ggacagcggc gcagagagag cggtgttctt cggattcctc gatccgcagg aactgctgga
    12001 ggcggtggat gcagcgggaa gggcggttac atatgaactg gatcgggatt cctgtaagct
    12061 tactgtggaa tcggcgggag cggagtatct gatgcttccg gtcaatacgg atagcggatg
    12121 gtattgtacg gtaaacggag aggagcgggc cataagccgg ctcgcgggca atctgatgct
    12181 cgtgccgctt gacgagggta cgaatgagat tacgctttgt tatgtgccgc ggggaatgaa
    12241 aaagggcgcg gcggtgacgg gcatggcact gatgcttctg gctggctggg gcgttttatc
    12301 acgtctgctt cggagaaaaa gtgtcctgga taaaatctac gccggacttg gctgtgccgc
    12361 cgcagatatt tttgtggtgg tctatgccgg atttctgctg gtcgtctatg tgattccggt
    12421 ggtgtatacg atatatttaa gatggatctg aggtaacaga atggaaaaat tacaagcgag
    12481 aaaaaaagta tatttgtggt tgctgatttt attgagcctt tgcgcgctga tcccgttagc
    12541 cgtactctcg aagtacaatc atcagtcggg ggatgactat ggttatgcct accgcacctt
    12601 ccagatctgg cgggagacac attctttctg gaatgtgata aaagaagcgt ttgccacatc
    12661 agcggagttc tatcagaagt ggcaggggct ttatgcatcg gcggttctgc tggcgctgga
    12721 accgggaatt ttcggcgaca aatattacgc gctgaccgga atcttgctga tcgcaattat
    12781 tgtgggggca aactgcgcgg gtacggttta tatctttcac cgcaaatgcg gctgcccgag
    12841 actggaggga tttgctgccg gctgtgtcat gtcgtttctt atgatcgaat ggatgccctc
    12901 cgctctgcag ggattgtact ggtataacgg cgcgatgaat tatgtattct tccatgcggt
    12961 gctattattg ttgttgtgtg tgctgctcag ccttgccggg gagcacccgc ggagaaaagc
    13021 aggcagggcg gtgcttgcgg tgctgcttgc ggtgctgctt gccggcggga atcacgtgac
    13081 cgcatttatg gggatactgc tgattgtcgg atttggaata tttgctgcgg taaaaaagcg
    13141 gcggcagaac ctgatctgct gcgtcataac gcttgcggct atggtggcgg gattcctttt
    13201 taatgtgacg tcgccgggca ccagaatccg gcagtcgtat ttttccgaca gacccggtgt
    13261 agcgggaacg attgttaccg cggtaaaaac cggaattgca tgcatcaaca gctggatggg
    13321 acttgcagtc attgtcacgg cggtcctgct gcttccggtg cttcttcccc tggtaaagaa
    13381 gatacgcgga cagtatggat tttcgttccg ctatccgctg cttgtctttg tgttgtcggt
    13441 cgcctggctg tgcgcaatgt tctgcccgcc catctacgcg atgggaacgc cgggggacgt
    13501 ccggctgaca aacgtggtat atttttcctt cgtttggctg tttgtgttaa atgaagccta
    13561 tctgtgcgga tgggcggtca caagactgga ggcgtccggg gcggcagcac tgtccggggc
    13621 tgcaggagaa tttccgggca gttttttgat cacggctgct gtccttgcgg tgggaatgat
    13681 aatcggatgc ggggaaaata tgaccgcatg gcaggcatac ctgctgatcc gcagcggcga
    13741 ggcacaggaa tattcggcgg aggctgatga gcgctacgcc gttttgtgtg attctgcggg
    13801 ggaagacgtg gtgttaaagg cgtataccgc aaagccgtgg atgcttttcc tcgacgatat
    13861 tacagaggag aaaagcgact ggagaaatga atatatgcgc gtctattttg gactgaacag
    13921 cgttgtgtta gagcagacgg aatgagacgg cagaagggag cagcggacac aaaaggcagg
    13981 ctgctccttt ttcataagac ccggttatag ggagtatgaa aaagaaaaat agattcagag
    14041 gttgcattgt ttcccgagat acattattat agaatttata tactgtctgt tagtaatgta
    14101 ctaaagtcaa tacaagacgg attcctgcac ggcaggaaga aaagagggat tagagagatg
    14161 gaatataagg taggcgtgat ttccggcgac gggatcggac cggagatcgt aacagaggca
    14221 aagaaagttc tggatcagat tggaaagacc tacggacatg tgttccgcta tgaagagatt
    14281 ctgatgggag gctgctccat tgacgcgacg ggtgagccgc tcacggatgc ggcgatcgct
    14341 tccgcgaagg cttccgatgc ggtgctgatg ggatcgatcg gcggcaacac atcgacttcg
    14401 ccgtggtatc agctgccgcc ggagaagaga ccggaggcgg gacttttaaa
//
