LOCUS       c00336_gnlPro..         2764 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|PPFIPPEA_336.
ACCESSION   c00336_gnlPro..
VERSION     c00336_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-07 23:10:07
            Original ID  :: gnl|Prokka|PPFIPPEA_336
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 2764
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..2764
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[138:2764]"
                     /cutoff="10000"
                     /detection_rule="(minimum(1, [HAAA1, HG2B1, HG2B2, HGD-D,
                     HleuD1, HleuD2, HleuS1, HleuS2, Lactoyl-CoA1,
                     Lactoyl-CoA2]) and minimum(1, [ACP_syn_III, ACP_syn_III_C,
                     AcetylCoA_hyd_C, AcetylCoA_hydro, Acyl-CoA_dh_1,
                     Acyl-CoA_dh_2, AdoHcyase_NAD, AlaDh_PNT_C, BcrAD_BadFG,
                     Biotin_lipoyl, Carboxyl_trans, CoA_trans,
                     CoA_trans_230_acto, CoA_trans_6_Glutaconate, CoA_transf_3,
                     ECH_1, ECH_2, ETF, ETF_alpha, FGGY_C, HCDH, HCDH_N,
                     Hacid_dh, Hacid_dh_C, Mal_decarbox_Al, Peptidase_M22,
                     Peptidase_M23, Thiolase_C, Thiolase_N, ketoacyl-synt]) and
                     not (minimum(2, [AcetylCoA_hyd_C, BcrAD_BadFG, CoA_trans,
                     CoA_trans_230_acto, Mal_decarbox_Al]) and Lactoyl-CoA2 and
                     Lactoyl-CoA1) and not (minimum(4, [ACP_syn_III,
                     ACP_syn_III_C, Acyl-CoA_dh_1, Acyl-CoA_dh_2, AlaDh_PNT_C,
                     BcrAD_BadFG, ECH_1, ECH_2, ETF, ETF_alpha, HCDH, HCDH_N,
                     Hacid_dh, Hacid_dh_C, IlvN, NAD_Gly3P_dh_N, Thiolase_C,
                     Thiolase_N, ketoacyl-synt]) and HAAA1 and not HleuS1))"
                     /neighbourhood="10000"
                     /product="Others_HGD_unassigned"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      139..2764
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="10000"
                     /detection_rule="(minimum(1, [HAAA1, HG2B1, HG2B2, HGD-D,
                     HleuD1, HleuD2, HleuS1, HleuS2, Lactoyl-CoA1,
                     Lactoyl-CoA2]) and minimum(1, [ACP_syn_III, ACP_syn_III_C,
                     AcetylCoA_hyd_C, AcetylCoA_hydro, Acyl-CoA_dh_1,
                     Acyl-CoA_dh_2, AdoHcyase_NAD, AlaDh_PNT_C, BcrAD_BadFG,
                     Biotin_lipoyl, Carboxyl_trans, CoA_trans,
                     CoA_trans_230_acto, CoA_trans_6_Glutaconate, CoA_transf_3,
                     ECH_1, ECH_2, ETF, ETF_alpha, FGGY_C, HCDH, HCDH_N,
                     Hacid_dh, Hacid_dh_C, Mal_decarbox_Al, Peptidase_M22,
                     Peptidase_M23, Thiolase_C, Thiolase_N, ketoacyl-synt]) and
                     not (minimum(2, [AcetylCoA_hyd_C, BcrAD_BadFG, CoA_trans,
                     CoA_trans_230_acto, Mal_decarbox_Al]) and Lactoyl-CoA2 and
                     Lactoyl-CoA1) and not (minimum(4, [ACP_syn_III,
                     ACP_syn_III_C, Acyl-CoA_dh_1, Acyl-CoA_dh_2, AlaDh_PNT_C,
                     BcrAD_BadFG, ECH_1, ECH_2, ETF, ETF_alpha, HCDH, HCDH_N,
                     Hacid_dh, Hacid_dh_C, IlvN, NAD_Gly3P_dh_N, Thiolase_C,
                     Thiolase_N, ketoacyl-synt]) and HAAA1 and not HleuS1))"
                     /neighbourhood="10000"
                     /product="Others_HGD_unassigned"
                     /protocluster_number="1"
     cand_cluster    1..2764
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(minimum(1, [HAAA1, HG2B1, HG2B2, HGD-D,
                     HleuD1, HleuD2, HleuS1, HleuS2, Lactoyl-CoA1,
                     Lactoyl-CoA2]) and minimum(1, [ACP_syn_III, ACP_syn_III_C,
                     AcetylCoA_hyd_C, AcetylCoA_hydro, Acyl-CoA_dh_1,
                     Acyl-CoA_dh_2, AdoHcyase_NAD, AlaDh_PNT_C, BcrAD_BadFG,
                     Biotin_lipoyl, Carboxyl_trans, CoA_trans,
                     CoA_trans_230_acto, CoA_trans_6_Glutaconate, CoA_transf_3,
                     ECH_1, ECH_2, ETF, ETF_alpha, FGGY_C, HCDH, HCDH_N,
                     Hacid_dh, Hacid_dh_C, Mal_decarbox_Al, Peptidase_M22,
                     Peptidase_M23, Thiolase_C, Thiolase_N, ketoacyl-synt]) and
                     not (minimum(2, [AcetylCoA_hyd_C, BcrAD_BadFG, CoA_trans,
                     CoA_trans_230_acto, Mal_decarbox_Al]) and Lactoyl-CoA2 and
                     Lactoyl-CoA1) and not (minimum(4, [ACP_syn_III,
                     ACP_syn_III_C, Acyl-CoA_dh_1, Acyl-CoA_dh_2, AlaDh_PNT_C,
                     BcrAD_BadFG, ECH_1, ECH_2, ETF, ETF_alpha, HCDH, HCDH_N,
                     Hacid_dh, Hacid_dh_C, IlvN, NAD_Gly3P_dh_N, Thiolase_C,
                     Thiolase_N, ketoacyl-synt]) and HAAA1 and not HleuS1))"
                     /kind="single"
                     /product="Others_HGD_unassigned"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..2764
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Others_HGD_unassigned"
                     /region_number="1"
                     /rules="(minimum(1, [HAAA1, HG2B1, HG2B2, HGD-D, HleuD1,
                     HleuD2, HleuS1, HleuS2, Lactoyl-CoA1, Lactoyl-CoA2]) and
                     minimum(1, [ACP_syn_III, ACP_syn_III_C, AcetylCoA_hyd_C,
                     AcetylCoA_hydro, Acyl-CoA_dh_1, Acyl-CoA_dh_2,
                     AdoHcyase_NAD, AlaDh_PNT_C, BcrAD_BadFG, Biotin_lipoyl,
                     Carboxyl_trans, CoA_trans, CoA_trans_230_acto,
                     CoA_trans_6_Glutaconate, CoA_transf_3, ECH_1, ECH_2, ETF,
                     ETF_alpha, FGGY_C, HCDH, HCDH_N, Hacid_dh, Hacid_dh_C,
                     Mal_decarbox_Al, Peptidase_M22, Peptidase_M23, Thiolase_C,
                     Thiolase_N, ketoacyl-synt]) and not (minimum(2,
                     [AcetylCoA_hyd_C, BcrAD_BadFG, CoA_trans,
                     CoA_trans_230_acto, Mal_decarbox_Al]) and Lactoyl-CoA2 and
                     Lactoyl-CoA1) and not (minimum(4, [ACP_syn_III,
                     ACP_syn_III_C, Acyl-CoA_dh_1, Acyl-CoA_dh_2, AlaDh_PNT_C,
                     BcrAD_BadFG, ECH_1, ECH_2, ETF, ETF_alpha, HCDH, HCDH_N,
                     Hacid_dh, Hacid_dh_C, IlvN, NAD_Gly3P_dh_N, Thiolase_C,
                     Thiolase_N, ketoacyl-synt]) and HAAA1 and not HleuS1))"
                     /tool="gutsmash"
     CDS             139..1410
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Others_HGD_unassigned: HGD-D"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg336_1"
                     /sec_met_domain="HGD-D (E-value: 6e-57, bitscore: 180.8,
                     seeds: 370, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MEIIETFGHYVENLTDKKPQSARRLLKTGWEAQNLKFRYMQDKRL
                     MPADRHLANLMMDTMLRPLQKPEDSVIVSVFTPCEMMQEVGLHPYNVEGFSCYLSASKA
                     ERAFLQQAENQGIAETLCSYHKTFLGAAQKGLLPKPKCIVYTNLTCDANLLTFRTLADF
                     YQVPVFAIDVPWNQTTENVQYVADQLKDLKIFLEKNTGKTISEDRLKERLACSKRTLEN
                     YKKYQQMRADRYVPSDLVTPLYAGMTNNILLGTAEEEKYTQMLLENIKKAPAAKGKHIY
                     WMHTIPFWSDAVRQELCFSEKAQIVGCELAETCEPDFDPENPFEAMAERMVYHSLNGSA
                     LRRIEAGIRHAKQAGADGAVWFGHWGCKHTLGAAQLAKKKFEEAGLPLLILDGDGCDRS
                     HGGEGQTSTRLGAFLEMLGGAENE"
     CDS             1403..2764
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Others_HGD_unassigned: BcrAD_BadFG"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Others_HGD_unassigned: HGD-D"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg336_2"
                     /sec_met_domain="HGD-D (E-value: 1.9e-18, bitscore: 54.4,
                     seeds: 370, tool: rule-based-clusters)"
                     /sec_met_domain="BcrAD_BadFG (E-value: 1.2e-44, bitscore:
                     139.8, seeds: 18, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNKIYYVCKYTPIELLESLGGECENFNHMPEGFDLADQVSHPNIC
                     GFGKTLLEGVMEGKIKELVLVSCCDTIRSAYDILLESGKLDFLYILDVLHCDSACSRER
                     MAVQLKGLAKAYAQYKGTEFDAGKFRAAFHAQEKITKSHIAVLGARMGQELFEMTSKAM
                     PLPVENDTCVHNRSVGNILPPEGASFDELMDWYAGEILGQIPCMRMMDPTGRKKLYNDP
                     SVAGIIYHTVKFCDFYSFEYAEIKNHTDVPLLKIESDYTIQSSGQLLTRLEAFAESIQP
                     ETLEQTIDGDTKGERKMGKGYFAGIDSGSTSTDVVILNKDHEIVTSIILPTGAGAAIGA
                     DRALAEALKEAGLQREDIDALVTTGYGRTAIKNGDKSITEITCHARGAHFLNPEVRTVI
                     DIGGQDSKVIRLDENGAVANFVMNDKCAAGTGRFLEMMARTMEMNLDQMSECGLE"
ORIGIN
        1 tcgcagaata gtggtgtgtg aacagtaacg aaaattcaac ttgacttcca aaaaagatat
       61 tattaaaata ttccgtggca ctatttgtgc attgacaatt gataaattat tattaccata
      121 atgaggaaga gacagactat ggagattatt gagacatttg gtcattatgt ggaaaatctg
      181 acggataaaa agcctcagtc agcccgccgg ctgttaaaaa ccggctggga ggcgcagaat
      241 ctgaagttcc gttatatgca ggacaagaga ctgatgcctg cagacaggca tcttgcaaat
      301 ctgatgatgg ataccatgct gcggcccctt cagaaaccgg aagattctgt tattgtaagt
      361 gtttttacac cctgcgaaat gatgcaggag gtgggattac atccctataa tgtagaagga
      421 ttttcctgct atctttctgc aagcaaagca gagagggcat ttttgcagca ggcagagaat
      481 cagggaattg cagagacact gtgcagttat cacaagacat ttcttggtgc agcgcagaaa
      541 ggacttctgc caaagcctaa atgtattgtt tataccaacc tgacctgcga tgctaacctg
      601 ctcaccttcc gtaccctggc agatttttac caggtaccgg tatttgccat tgatgtacca
      661 tggaatcaga ccacagagaa tgtacagtat gtggcagatc agttaaagga tttaaaaata
      721 tttctggaaa aaaatacagg aaaaacaatc tctgaggaca gattaaagga acgtcttgcc
      781 tgcagtaagc ggactctgga gaattacaaa aaatatcagc agatgcgtgc agacagatat
      841 gtgccctctg atctggtcac acccttgtat gcaggaatga caaacaatat ccttctggga
      901 acagcagaag aagagaagta tacacagatg cttcttgaaa atattaagaa agcacctgca
      961 gcgaaaggga aacacattta ctggatgcat accattccat tctggtcgga tgcagtgcgc
     1021 caggagcttt gcttcagtga gaaagcccag atcgtaggat gtgaacttgc agaaacctgt
     1081 gagccggatt ttgatcctga gaaccctttt gaagcaatgg cagaaagaat ggtatatcat
     1141 tcactgaacg gaagtgccct tagaaggatt gaagccggta tccgccatgc gaaacaggca
     1201 ggagcagacg gtgctgtgtg gtttggacat tggggatgta aacatacgct tggtgcggca
     1261 cagcttgcaa agaagaagtt cgaggaagcc ggtctgcccc ttttgatcct ggacggtgac
     1321 gggtgtgatc gaagtcatgg aggagaagga cagacttcca ccaggctggg agcttttctg
     1381 gaaatgctgg gaggtgctga gaatgaataa aatttactat gtctgtaaat ataccccaat
     1441 tgaacttctg gaatctcttg gcggggaatg tgagaacttt aatcatatgc cggagggatt
     1501 tgacctggcc gatcaggttt cacatcccaa tatctgcggt tttggaaaaa cacttctgga
     1561 aggagtcatg gagggaaaaa tcaaggaact tgtcctggtg agctgctgtg atactatccg
     1621 gagtgcctat gacattctgc tggaaagcgg caagctggat tttctctata ttttggatgt
     1681 gcttcactgt gacagtgcct gcagcagaga gcgtatggct gtacagttaa aagggctggc
     1741 gaaggcttat gcacagtaca aaggcacaga gtttgatgcc ggaaaattcc gggctgcttt
     1801 ccatgcacag gagaaaatca caaagtccca tattgcagtg cttggcgcca gaatggggca
     1861 ggaattattt gaaatgacca gcaaggctat gcctcttcct gtagagaatg atacctgcgt
     1921 gcataaccgt tctgtgggaa atatacttcc accggagggt gcttcttttg atgagctgat
     1981 ggactggtat gcaggtgaga ttctggggca gattccatgt atgcgtatga tggacccgac
     2041 aggtcgtaag aagctttata atgatccgtc tgttgcgggg attatttatc atacagtgaa
     2101 gttttgtgat ttttacagct tcgaatatgc tgagattaag aatcatacag atgtacctct
     2161 tttaaaaatc gaatcagatt atactattca gagtagcggt cagcttctta ccagactgga
     2221 agcttttgca gagagcattc agcctgaaac actggagcag accatagatg gagacacaaa
     2281 aggagagaga aaaatgggaa aaggttattt cgcagggatt gacagtggtt ccaccagtac
     2341 agatgtggtg attttaaata aagatcatga gattgtgacc agtattatcc tgcctacagg
     2401 agcaggagct gctatcggag cagaccgtgc ccttgcagag gcattgaagg aggccggatt
     2461 gcagagagaa gatattgatg cgctggttac taccggatac ggacgtacag ctattaaaaa
     2521 tggtgataag agtatcacag agatcacctg tcatgcaaga ggtgcgcatt tcctgaatcc
     2581 ggaagtccgt actgttattg atattggcgg acaggacagc aaggtgatcc gactggatga
     2641 gaacggtgca gttgcaaact ttgttatgaa tgacaaatgt gcggcaggaa ccggacgatt
     2701 tctggaaatg atggcacgta ccatggaaat gaatctggat cagatgagcg aatgtgggct
     2761 ggaa
//
