GCA_958403025.1 - 4 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

gnlProkkaBLIBGPPP_5 (original name was: gnl|Prokka|BLIBGPPP_5)

Region	Type	From	To	Most similar known cluster		Similarity
Region 5.1	NRPS	1	33,100	dipeptide aldehydes	NRP	23%

gnlProkkaBLIBGPPP_11 (original name was: gnl|Prokka|BLIBGPPP_11)

Region	Type	From	To	Most similar known cluster		Similarity
Region 11.1	ranthipeptide	179	21,767

gnlProkkaBLIBGPPP_12 (original name was: gnl|Prokka|BLIBGPPP_12)

Region	Type	From	To	Most similar known cluster		Similarity
Region 12.1	lanthipeptide-class-ii	2,108	24,810

gnlProkkaBLIBGPPP_58 (original name was: gnl|Prokka|BLIBGPPP_58)

Region	Type	From	To	Most similar known cluster		Similarity
Region 58.1	cyclic-lactone-autoinducer	228	11,171

Compact view

gnlProkkaBLIBGPPP_5 - Region 1 - NRPS

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Location: 1 - 33,100 nt. (total: 33,100 nt) Show pHMM detection rules used

Region on contig edge.

NRPS: cds(Condensation and (AMP-binding or A-OX))

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

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Gene details

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Gene overview

NRPS/PKS domains

KnownClusterBlast

NRPS/PKS modules

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Detailed domain annotation

Selected features only

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Similar known gene clusters from MIBiG 3.1

Module view

Legend

NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 0 - 33100:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X - X - X)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

ctg5_4

AMP-binding (22..421): X

nrpys: Leu, Trp

SVM prediction details:

Predicted physicochemical class:: N/A
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Tiny, hydrophilic, transition to aliphatic (Gly, Ala)
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DVLTDGGISK
Nearest Stachelhaus code(s):: Leu or Trp DVLTDGGISK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (1035..1423): X

nrpys: Leu, Trp

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DAMFSGAIFK
Nearest Stachelhaus code(s):: Leu or Trp DAMFSGAIFK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (2029..2420): X

nrpys: Leu, Trp

SVM prediction details:

Predicted physicochemical class:: N/A
Large clusters prediction:: Aromatic side chain (Phe, Trp, Ph-Gly, Tyr, bOH-Tyr)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DAITTGGVSK
Nearest Stachelhaus code(s):: Leu or Trp DAITTGGVSK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

gnlProkkaBLIBGPPP_11 - Region 1 - ranthipeptide

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Location: 179 - 21,767 nt. (total: 21,589 nt) Show pHMM detection rules used

Region on contig edge.

ranthipeptide: (TIGR03973 or SCIFF or (cds(PF04055 and (TIGR04085 or SPASM)) and minimum(1, [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE, Cytolysin_C_RRE, Goadsporin_CD_RRE, Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE, Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE, Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE, Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE, Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE, Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE, Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE, Thiaglutamate_B_RRE, Thiaglutamate_G_RRE, Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE, Thurincin_rSAM_RRE, Trifolitoxin_RRE]) and not (TIGR01716 or subtilosin or TIGR02111 or TIGR03966 or TIGR04103 or (YcaO or TIGR03604))))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

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Gene details

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Gene overview

KnownClusterBlast

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Similar known gene clusters from MIBiG 3.1

No matches found.

gnlProkkaBLIBGPPP_12 - Region 1 - lanthipeptide-class-ii

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Location: 2,108 - 24,810 nt. (total: 22,703 nt) Show pHMM detection rules used

Region on contig edge.

lanthipeptide-class-ii: cds(LANC_like and DUF4135)

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core biosynthetic genes

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Gene overview

KnownClusterBlast

Lanthipeptides

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Similar known gene clusters from MIBiG 3.1

No matches found.

Lanthipeptide predictions

Lanthipeptide(s) for ctg12_13

ctg12_12

Leader:

MDEQKMNGDILKELEEIEKIETEDIEAV

Core:

HSMGLGVVSIICC

Core with dehydrations:

HDhaMGLGVVDhaIICysCys

antiSMASH-DB 4.0 matches for ctg12_12

100.0% NZ_CZBP01000025.1: Lanthipeptides: allorf_41775_41900_lanthipeptide

1

HSMGLGVVSIICC

13

HSMGLGVVSIICC

1

HSMGLGVVSIICC

13

allorf_03633_03746

Leader:

MTCCLMVMNIRKALSAG

Core:

KIHPTERASVFCPYFLVSGL

Core with dehydrations:

KIHPDhbERADhaVFCysPYFLVDhaGL

antiSMASH-DB 4.0 matches for allorf_03633_03746

100.0% NZ_JADMYT010000009.1and 3 others:

NZ_JADNMI010000013.1 Lanthipeptides: allorf_010454_010567_lanthipeptideNZ_QUEF01000006.1 Lanthipeptides: allorf_116827_116940_lanthipeptideNZ_QUIT01000007.1 Lanthipeptides: allorf_004751_004864_lanthipeptide

Lanthipeptides: allorf_115889_116002_lanthipeptide

1

KIHPTERASVFCPYFLVSGL

20

KIHPTERASVFCPYFLVSGL

1

KIHPTERASVFCPYFLVSGL

20

allorf_22795_22938

Leader:

MFSSCLNFQPGIALKASRAG

Core:

NLHVIGTLNLCFSHTHIMFFRKFKSCL

Core with dehydrations:

NLHVIGDhbLNLCysFDhaHDhbHIMFFRKFKDhaCysL

antiSMASH-DB 4.0 matches for allorf_22795_22938

18.5% NC_015215.1: Lanthipeptides: allorf_0087146_0087331_lanthipeptide

4

VIGTLNLCFSHTHI

17

V+G L LC+ ++++

2

VVGILPLCYGYSNL

15

Legend:

Dha: Didehydroalanine
Dhb: Didehydrobutyrine

Lanthipeptides

Core peptide predictions

ctg12_12 - Class II

Cleavage pHMM score: -5.70

RODEO score: 0

Monoisotopic mass: 1281.6 Da

Molecular weight: 1282.6 Da

Number of bridges: 2

allorf_03633_03746 - Class II

Cleavage pHMM score: -5.50

RODEO score: 0

Monoisotopic mass: 2209.2 Da

Molecular weight: 2210.6 Da

Number of bridges: 1

Alternative weights: (assuming N unmodified Ser/Thr residues); 2228.6 Da N = 1; 2246.6 Da N = 2

allorf_22795_22938 - Class II

Cleavage pHMM score: -6.30

RODEO score: 0

Monoisotopic mass: 3133.7 Da

Molecular weight: 3135.8 Da

Number of bridges: 2

Alternative weights: (assuming N unmodified Ser/Thr residues); 3153.8 Da N = 1; 3171.8 Da N = 2

gnlProkkaBLIBGPPP_58 - Region 1 - cyclic-lactone-autoinducer

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Location: 228 - 11,171 nt. (total: 10,944 nt) Show pHMM detection rules used

Region on contig edge.

cyclic-lactone-autoinducer: (TIGR04223 or AgrB)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

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Gene details

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Gene overview

KnownClusterBlast

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Similar known gene clusters from MIBiG 3.1

No matches found.