LOCUS       c00194_gnlPro..         6651 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|MHIJKGIP_194.
ACCESSION   c00194_gnlPro..
VERSION     c00194_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-07 18:44:30
            Original ID  :: gnl|Prokka|MHIJKGIP_194
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 6651
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..6651
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[2:5610]"
                     /cutoff="10000"
                     /detection_rule="(TPP_enzyme_C and not (PFL-like or
                     PFL_6_pcresol or PFL_9_tma or Gly_radical or Gly_430_pcre
                     or Gly_8-9_TMA or PFL_acetate1 or PFL_acetate2 or
                     Gly_radical_scatole or PFL_proline or PFL_toluene) and
                     minimum(2, [ALS_ss_C, AlaDh_PNT_C, Arginase, BKACE,
                     BcrAD_BadFG, Beta_elim_lyase, CPSase_L_D2, Cys_Met_Meta_PP,
                     ELFV_dehydrog, GRDB, Glu_syn_central, Glu_synthase,
                     Gly_reductase, HAAA1, HG2B1, HG2B2, HGD-D, HleuD1, HleuD2,
                     HleuS1, HleuS2, Homoserine_dh, ILVD_EDD, IlvC,
                     Lactoyl-CoA1, Lactoyl-CoA2, Lyase_aromatic, MAAL_C,
                     OKR_DC_1_5_lysine, OKR_DC_1_5_ornithine,
                     OKR_DC_1_7_arginine, OKR_DC_1_C, OTCace, PALP,
                     Proline_reductase, Sacchrp_dh_C, TPL, Thr_dehydrat_C, TnaA,
                     Urocanase]) and not (POR or POR_N or PFOR_II))"
                     /neighbourhood="10000"
                     /product="TPP_AA_metabolism"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      3..5610
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="10000"
                     /detection_rule="(TPP_enzyme_C and not (PFL-like or
                     PFL_6_pcresol or PFL_9_tma or Gly_radical or Gly_430_pcre
                     or Gly_8-9_TMA or PFL_acetate1 or PFL_acetate2 or
                     Gly_radical_scatole or PFL_proline or PFL_toluene) and
                     minimum(2, [ALS_ss_C, AlaDh_PNT_C, Arginase, BKACE,
                     BcrAD_BadFG, Beta_elim_lyase, CPSase_L_D2, Cys_Met_Meta_PP,
                     ELFV_dehydrog, GRDB, Glu_syn_central, Glu_synthase,
                     Gly_reductase, HAAA1, HG2B1, HG2B2, HGD-D, HleuD1, HleuD2,
                     HleuS1, HleuS2, Homoserine_dh, ILVD_EDD, IlvC,
                     Lactoyl-CoA1, Lactoyl-CoA2, Lyase_aromatic, MAAL_C,
                     OKR_DC_1_5_lysine, OKR_DC_1_5_ornithine,
                     OKR_DC_1_7_arginine, OKR_DC_1_C, OTCace, PALP,
                     Proline_reductase, Sacchrp_dh_C, TPL, Thr_dehydrat_C, TnaA,
                     Urocanase]) and not (POR or POR_N or PFOR_II))"
                     /neighbourhood="10000"
                     /product="TPP_AA_metabolism"
                     /protocluster_number="1"
     cand_cluster    1..6651
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(TPP_enzyme_C and not (PFL-like or
                     PFL_6_pcresol or PFL_9_tma or Gly_radical or Gly_430_pcre
                     or Gly_8-9_TMA or PFL_acetate1 or PFL_acetate2 or
                     Gly_radical_scatole or PFL_proline or PFL_toluene) and
                     minimum(2, [ALS_ss_C, AlaDh_PNT_C, Arginase, BKACE,
                     BcrAD_BadFG, Beta_elim_lyase, CPSase_L_D2, Cys_Met_Meta_PP,
                     ELFV_dehydrog, GRDB, Glu_syn_central, Glu_synthase,
                     Gly_reductase, HAAA1, HG2B1, HG2B2, HGD-D, HleuD1, HleuD2,
                     HleuS1, HleuS2, Homoserine_dh, ILVD_EDD, IlvC,
                     Lactoyl-CoA1, Lactoyl-CoA2, Lyase_aromatic, MAAL_C,
                     OKR_DC_1_5_lysine, OKR_DC_1_5_ornithine,
                     OKR_DC_1_7_arginine, OKR_DC_1_C, OTCace, PALP,
                     Proline_reductase, Sacchrp_dh_C, TPL, Thr_dehydrat_C, TnaA,
                     Urocanase]) and not (POR or POR_N or PFOR_II))"
                     /kind="single"
                     /product="TPP_AA_metabolism"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..6651
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="TPP_AA_metabolism"
                     /region_number="1"
                     /rules="(TPP_enzyme_C and not (PFL-like or PFL_6_pcresol or
                     PFL_9_tma or Gly_radical or Gly_430_pcre or Gly_8-9_TMA or
                     PFL_acetate1 or PFL_acetate2 or Gly_radical_scatole or
                     PFL_proline or PFL_toluene) and minimum(2, [ALS_ss_C,
                     AlaDh_PNT_C, Arginase, BKACE, BcrAD_BadFG, Beta_elim_lyase,
                     CPSase_L_D2, Cys_Met_Meta_PP, ELFV_dehydrog, GRDB,
                     Glu_syn_central, Glu_synthase, Gly_reductase, HAAA1, HG2B1,
                     HG2B2, HGD-D, HleuD1, HleuD2, HleuS1, HleuS2,
                     Homoserine_dh, ILVD_EDD, IlvC, Lactoyl-CoA1, Lactoyl-CoA2,
                     Lyase_aromatic, MAAL_C, OKR_DC_1_5_lysine,
                     OKR_DC_1_5_ornithine, OKR_DC_1_7_arginine, OKR_DC_1_C,
                     OTCace, PALP, Proline_reductase, Sacchrp_dh_C, TPL,
                     Thr_dehydrat_C, TnaA, Urocanase]) and not (POR or POR_N or
                     PFOR_II))"
                     /tool="gutsmash"
     CDS             3..1721
                     /gene_functions="biosynthetic (rule-based-clusters)
                     TPP_AA_metabolism: TPP_enzyme_C"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg194_1"
                     /sec_met_domain="TPP_enzyme_C (E-value: 2.8e-49, bitscore:
                     155.5, seeds: 323, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKQISGNKLLVKALKEEGVEYLFGYPGACTIDISDELYKQDDVKI
                     ILPRHEQALVHEADAYARTTGKVGVCLVTSGPGATNLVTGLATANYDSVPLVCFTGQVA
                     RHLIGNDAFQEVDIVGITRSITKYGVTVRRREDLGRIIKEAFYIARTGRPGPVLIDLPK
                     DVMAELGSAEYPKNVNIRGYKPNTDVHIGQLKRAIKLLNKAKRPLFLAGGGVVISRAHE
                     IFREVVEKTKVPVVTTVMGKGSIPTDHPLYIGNLGMHGAYAANMAVSNCDVLFSIGTRF
                     NDRITGKLHEFAPHAQIIHIDIDTASISRNIQVDIPIVADAKEAITKMNEYVQECSTDK
                     WLNLIKNWKEEHPLKMRPNDLLSPMDILKEINEQFENSIIVTDVGQHQMLVSQYAEITE
                     GKQMIMSGGLGTMGYGLPGGIGAKIGNPDRPVIVISGDGGVQMNIQELATAVLEELPVI
                     LCIFNNEYLGMVRQWQKLFYGKRYAMTNLRAGALSRRTEGMEYPQYTPDFIRLAESYRA
                     KGIRVTKKEEIAAAFEEAKKNTKAPTVIEFIIDPEEMVYPMIKPGGTLEDMIMDC"
     CDS             1739..2248
                     /gene_functions="biosynthetic (rule-based-clusters)
                     TPP_AA_metabolism: ALS_ss_C"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg194_2"
                     /sec_met_domain="ALS_ss_C (E-value: 2.6e-23, bitscore:
                     70.9, seeds: 685, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDKGMKKRWISLYVENQVGVLSKISGLFSGKSYNLDSLTVGTTED
                     PTVSRMTIATVSDDETFEQIKKQLNRMIEVIKVIDFTDVFVRMKEILYIKVFNCSKEDK
                     VELFQIAETFKAKVIDYGKDSLLLEFVQTATKNDAVVKLIKEEFHRIEVVRGGSVGIES
                     ISMMER"
     CDS             complement(2317..3573)
                     /locus_tag="ctg194_3"
                     /transl_table=11
                     /translation="VKSTERLIFHIDVNSAFLSWESARRVSQGLPDLREIPSCVGGDPK
                     KRTGIVVAKSIPAKKYGIQTGEPVAMALRKCPNLVIVPSDFELYDKCSRAFKAICASFA
                     PVMESFSIDEVFLDMTGTSLIYPDPVAAAHELKDKIHRELGFTVNVGISTNKLLAKMAS
                     DFQKPDRVHTLFPDEIPEKMWPLPIRNLLFLGKASEQKLLNQGIRTIGDLARERASNVQ
                     YWLGEKTGHQLWQYARGIDDSPVKAVPDEAKGFSVETTFNDDITSIEQVFPILLEQCDV
                     LATRMRRKDKKCNCISVTFRTLDFRNRSHQTKLENATDLTDEIYINATRLFKEFWKGQP
                     LRLIGVALTGLTDGTYEQMSLFEDTENKERRRKLDAAMDEIRMKFGNDKITRASIMNSN
                     ARIGRKAKAQMKNEKTEEP"
     CDS             4336..5049
                     /locus_tag="ctg194_4"
                     /transl_table=11
                     /translation="MYLSDIHTHSIASGHGTTCTISDMAKAASKKGLKLLGISDHGPGT
                     LASGTSSYFRSLPFYPRKRFGIDILYGVELNILDSAGHTDLSDELLNNLDYAIISMHRQ
                     NYRSGSVSQNTEAYINAIKHPAVKILGHCDDTHFPVDYETLARAALRENVIFEINEASL
                     APGGYRGDTRANAARILYLCQKYQLPVILSSDSHGKEHVGDFTYAEEFVHQLMFPETLI
                     LNNQLPRLKVFLQTR"
     CDS             5251..5610
                     /gene_functions="biosynthetic (rule-based-clusters)
                     TPP_AA_metabolism: ALS_ss_C"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg194_5"
                     /sec_met_domain="ALS_ss_C (E-value: 3e-23, bitscore: 70.7,
                     seeds: 685, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTIATVSDDETFEQIKKQLNRMIEVIKVIDFTDVFVRMKEILYIK
                     VFNCSREDKAELFQIAETFKAKVIDYGKDSLLLEFVQTAIKNDAVVKLIKEEFHRIEVV
                     RGGSVGIESISMMER"
     CDS             5985..6620
                     /locus_tag="ctg194_6"
                     /transl_table=11
                     /translation="MNTTTTTTTAAAKRSSTLYMVELAMMIAIILLMSFTPLGYLRTPG
                     LSITLLTIPVAVGAIILGPKGGAVCGLAFGATSFYMAVTGSSAFAAALFNINPFGTFIV
                     CIVARVLEGWLTGLIFAALYKSPAKKFSYYIASLACPLLNTFFFMGFLCLFFYNTDYIQ
                     GIVSGLGVSNPVVFVAAFVGVQGLIEAGFCFVVGSIVSKALSVALKRA"
ORIGIN
        1 atatgaaaca aatatccggc aacaaactct tagtcaaagc cttaaaagaa gaaggcgtag
       61 aatacctctt cggctacccc ggagcctgta ccatcgacat cagtgatgaa ctctacaaac
      121 aggacgacgt caaaatcatc ctcccccgtc atgaacaggc ccttgtccac gaagccgacg
      181 cctacgccag aaccaccggc aaagtcggcg tatgtctggt aaccagcggt cccggagcaa
      241 ccaaccttgt aacaggactt gcaaccgcca actatgacag cgtacctctt gtatgcttca
      301 ccggccaggt agcccgccac ctcatcggca acgatgcatt tcaggaagta gacatcgtag
      361 gaatcaccag aagcatcacc aaatacggcg ttactgtccg cagacgtgaa gacctgggac
      421 gtatcatcaa agaagccttc tacattgccc gcaccggccg tccgggacct gtccttatcg
      481 accttccaaa ggacgtaatg gcagaactcg gcagcgctga atatcctaaa aatgtaaaca
      541 tccgcggata caagcccaac acagatgtcc acattggcca gctcaaacgt gccatcaaac
      601 ttttaaacaa ggcaaaacgt cctttattcc ttgcaggcgg cggtgtagta atctcccgcg
      661 cccacgaaat cttccgggaa gtagtagaaa agacaaaagt accggttgtc acaaccgtaa
      721 tgggaaaagg ctctatcccc acagaccatc cgctctatat cggaaacctt ggcatgcatg
      781 gtgcctatgc agcaaacatg gcagtcagca actgcgatgt gctgttctcc atcggaacca
      841 gattcaatga cagaatcacc ggaaaacttc atgaatttgc accacatgcc cagattatcc
      901 acattgatat tgatacagca tccatttccc gaaacattca ggtggatatc cccattgttg
      961 ctgatgcaaa agaggctatc accaaaatga acgaatacgt ccaggaatgt tcaacggaca
     1021 agtggcttaa cctgatcaaa aactggaaag aagagcatcc gcttaaaatg cgtccaaatg
     1081 atcttttaag tccgatggat atcctgaaag agatcaacga acagtttgag aacagtatca
     1141 tcgtaacaga tgtaggacaa catcagatgc ttgtctccca gtatgcggaa atcacagagg
     1201 gcaaacagat gatcatgtcc ggtggtctgg gaacaatggg ctatggtctt ccaggcggca
     1261 tcggagccaa aatcggaaat cccgacagac ctgtcatcgt tatctccggt gacggcggtg
     1321 tgcagatgaa cattcaggag cttgcaactg cagttctgga agaacttcct gttatcctgt
     1381 gcatctttaa caatgaatac cttggaatgg tacgccagtg gcagaagctt ttctacggca
     1441 aacgctatgc tatgaccaat ctgcgcgcag gagcactttc ccgccgcact gaaggcatgg
     1501 aatatccaca gtacacaccg gatttcatca gacttgcaga gagctacagg gcaaaaggaa
     1561 tccgtgtcac aaagaaagag gaaattgcag cagccttcga ggaagcaaaa aagaacacaa
     1621 aggcacctac agtaattgaa ttcatcattg accctgagga aatggtttat cctatgatca
     1681 aaccaggcgg aactctagaa gatatgatta tggactgttg atcaggagga aaattattat
     1741 ggataaagga atgaaaaaaa gatggatttc tctctatgtt gagaaccagg taggtgttct
     1801 gtccaaaatc tccggacttt tctccggaaa aagctacaat ctggacagtc ttactgtagg
     1861 aactaccgag gaccccacag tatctcgaat gactatcgca actgtcagtg atgatgaaac
     1921 ttttgagcag atcaaaaaac agttgaaccg tatgatcgag gttattaagg ttattgattt
     1981 cacagacgta tttgtccgca tgaaagagat tctgtatatt aaggttttta actgttccaa
     2041 agaagataag gttgaactgt ttcagattgc cgaaaccttc aaggcaaagg tcattgacta
     2101 tggcaaagac agtctgctcc tggaatttgt ccagacagca accaagaatg acgcagtagt
     2161 gaaactgatc aaggaagaat ttcacagaat tgaagtggta cgtggaggaa gtgtaggaat
     2221 cgaatctatc agtatgatgg aacgataaaa ctttttctga aatttttata aagggaggat
     2281 tgtcgcatta ttcttaatgc tcagtcctcc ctttacttac ggttcttctg tcttttcgtt
     2341 tttcatctgt gctttcgctt ttcgcccaat ccgtgcattg ctgttcataa tactggcacg
     2401 ggtgatctta tcatttccaa atttcatccg gatctcatcc atagctgcat ccagctttct
     2461 gcggcgttct ttattttctg tatcttcaaa cagagacatc tgttcataag tgccgtctgt
     2521 aaggcctgtc agcgccacac caatcagacg tagcggctgt cctttccaga attctttaaa
     2581 aagtcttgta gcatttatat aaatttcatc tgtaagatct gtagcatttt ccagctttgt
     2641 ctggtgtgaa cggtttctga aatccaaagt acggaaagtc acggaaatac agttgcattt
     2701 cttatctttt ctgcgcattc tggttgccag aacatcacac tgctccaaaa gtatgggaaa
     2761 cacctgctct atggaagtaa tatcatcgtt aaaagtagtt tccacactga aaccttttgc
     2821 ctcatcaggc actgctttta caggggaatc atcaatacct ctggcatatt gccatagctg
     2881 atgtcccgtt ttttccccaa gccagtactg cacattggat gctctttctc ttgccagatc
     2941 accgatggta cggatcccct gattcagaag cttctgctcg gaagcttttc ccagaaacag
     3001 cagattacgg ataggaagag gccacatttt ctccgggatt tcatccggga acagagtgtg
     3061 tactctgtcc ggcttttgaa aatctgatgc catctttgcc aaaagcttat tggtagaaat
     3121 tcctacattt actgtgaatc ccagctctct gtgaatcttg tctttcagct catgagcagc
     3181 agctacaggg tcaggataga tcagagaagt acctgtcata tccagaaata cctcatcaat
     3241 ggaaaaagat tccatcacag gtgcaaagga agcacagatt gctttaaatg cccgggaaca
     3301 tttatcgtat aattcaaaat cactgggaac gatcaccaga ttgggacatt tccgaagagc
     3361 catggctact ggctcgccgg tctgaatccc gtattttttt gcagggatag atttggcaac
     3421 cacgatacct gtccgtttct ttggatctcc tcctacacag gaaggaattt ctctgagatc
     3481 cggcagcccc tggctcaccc ttctggctga ttcccaggaa agaaatgcac tgtttacatc
     3541 aatatgaaaa attaaccgtt ccgtactttt cactgttctt cctccaatat atgaattgcg
     3601 ctcttcatat ctgatgattt cattttaccc tgatctgctc tccagggtaa gaacctcgtt
     3661 tgtccaaaaa agtgtcttta tttgcatcat cttgtctgag ctgttactgt atttatattt
     3721 gtttgatttc actctgctat cagagttttg aacaacaacg ccgcacttgc catacggcag
     3781 tgcggcgtct gtatttcgct accggtcaca ttgttcccgg taactttttt tacttaattc
     3841 tgaggattca tttcctctcg atatatactt atcgattagt cataaaggtt aggagcaatt
     3901 tcttcgctgt ccatatttgt ggaatcatac cagtatccag ttcagcctcc agccattcat
     3961 aagagaattc cgggaatgtc tgatgtttga aatttttcca tgttactccc ttctcatgaa
     4021 gtaactgtct acacataagc aaacagtgct atcgctgtca ttgatcagca ataagccccc
     4081 gtgttttcat atagttccat tataccttct cattttcccg aagtcaacat aatttaacat
     4141 gtatcagcca tttatttatg ttgtttttta tttgattttt gttgtatttc agttgttttt
     4201 attttgtttc ctcattgaca tttaaatcct catctgtcat acttgaatat aacgaatgta
     4261 ccatcaaaca gcagctttgc atctcccgta aggctcttat catatataga aaccgctctg
     4321 gaaaggagac aaacaatgta tctttctgac atccacacac attctatcgc cagcggtcac
     4381 ggaaccacct gtaccatctc tgacatggca aaagcagcca gcaaaaaagg actgaaactc
     4441 cttgggattt ctgaccacgg cccgggaact ctggcttccg gaacttcttc ttatttcaga
     4501 agccttcctt tttatcccag aaaaagattc ggcatagata ttctttacgg agttgaacta
     4561 aacatcctgg acagtgcagg acatacagat ctgtcagatg aactgctgaa taacctggat
     4621 tacgctatca tcagtatgca tcgacagaac taccgttcag gctctgtatc tcagaataca
     4681 gaagcttaca tcaatgcaat aaaacatcct gctgtaaaaa tactcggcca ctgcgacgat
     4741 acacatttcc ctgtagatta tgaaacgctg gcaagagcag ctttacggga aaatgttatt
     4801 tttgaaataa acgaagcttc cctcgcaccc ggaggatatc ggggagacac aagagccaat
     4861 gccgccagaa tcctgtatct ctgccagaaa tatcagcttc ctgttatttt gtccagcgac
     4921 agccacggaa aagaacatgt tggagatttc acctatgccg aagaattcgt tcaccagctc
     4981 atgtttccgg aaactcttat ccttaacaac cagcttccca gattaaaagt tttccttcag
     5041 acacgttaaa agaatgttcc tcaaagggac attcttttat tttatgcatt gttttttgca
     5101 ttgtgttatt ttgtttattt tctataattt cttatcaaaa aagacgacat tttagtacat
     5161 tttaattaac aaaactcact taaccattta catatctcgt gatagatcag tcttactgta
     5221 ggaaccaccg aggatcctac agtatcccga atgactatcg caacagtcag cgatgatgaa
     5281 acctttgaac agatcaaaaa acaattaaac cgcatgatcg aggttatcaa ggtaatcgac
     5341 ttcacagatg tatttgtcag aatgaaagaa attctctaca tcaaggtttt caactgctcc
     5401 agggaagata aagcagaact gtttcagatt gcagaaacct tcaaggcaaa agtcattgac
     5461 tacggcaaag acagcctgct tctggaattt gtccagacag ccatcaagaa tgatgcagtt
     5521 gtcaaactga tcaaggaaga attccacaga atcgaagtag tacgtggtgg aagtgtggga
     5581 attgaatcca tcagcatgat ggaacgctaa atctcataat caatattttt tgcagaatat
     5641 agaaacgtct ctatatcctg tatttatttt caaacatact ataaaatatt gcagttttta
     5701 atcaaatgtg ttattgttta cgggagcgtt attcgaaaga aaatgcatct gatttccaca
     5761 tctgacagtc tggtggataa agtcatccac ttcacagtct agttaaattt gtgaggcgac
     5821 ctgtaaggcg tctacacacc ggaaactttt accggaagat atttcttgtt tcatatcttt
     5881 tgttctgatg tatatttttc ataacgcctg atccccatac tctaaactgt ctgaaaagca
     5941 ggggacgcat tgcgccgttt tatcagacca aaaggagaat tcatatgaac acaacaacaa
     6001 ccactacaac agctgccgca aaaagaagca gcactcttta catggtagag ctggctatga
     6061 tgatcgccat tattctgctc atgtccttta ccccgctggg ctaccttcgt actcccggct
     6121 tatccatcac tcttctcacc atcccggtag ctgtaggagc tatcatcctc ggaccgaaag
     6181 gtggtgctgt atgcggactt gctttcggtg caaccagctt ctatatggct gtcacaggaa
     6241 gttcagcttt tgctgccgca cttttcaaca tcaatccatt tggtactttt attgtatgta
     6301 tcgtggcacg tgtgctggaa ggatggctta caggacttat tttcgcagca ttatataaat
     6361 caccggcaaa gaaattttcc tattacattg cttcactggc atgcccgtta ctgaatactt
     6421 tcttcttcat gggcttttta tgtctcttct tctacaacac agattacatt cagggtattg
     6481 tttccggtct tggagtaagc aatcctgttg tcttcgttgc tgcttttgtg ggcgttcagg
     6541 gactgatcga agcgggcttc tgctttgtag tgggaagtat cgtttccaaa gcactttctg
     6601 tggcactgaa acgtgcataa tctttaaaag aacagccttt tccaaaaaac c
//
