LOCUS       c00208_gnlPro..         6374 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|MHIJKGIP_208.
ACCESSION   c00208_gnlPro..
VERSION     c00208_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-07 18:44:30
            Original ID  :: gnl|Prokka|MHIJKGIP_208
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 6374
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..6374
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[1562:4811]"
                     /cutoff="10000"
                     /detection_rule="(minimum(2, [AdoMet_dc, Spermine_synt_N,
                     Spermine_synth]) or (Orn_DAP_Arg_deC and Sacchrp_dh_C))"
                     /neighbourhood="10000"
                     /product="Putrescine2spermidine"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      1563..4811
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="10000"
                     /detection_rule="(minimum(2, [AdoMet_dc, Spermine_synt_N,
                     Spermine_synth]) or (Orn_DAP_Arg_deC and Sacchrp_dh_C))"
                     /neighbourhood="10000"
                     /product="Putrescine2spermidine"
                     /protocluster_number="1"
     cand_cluster    1..6374
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(minimum(2, [AdoMet_dc, Spermine_synt_N,
                     Spermine_synth]) or (Orn_DAP_Arg_deC and Sacchrp_dh_C))"
                     /kind="single"
                     /product="Putrescine2spermidine"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..6374
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Putrescine2spermidine"
                     /region_number="1"
                     /rules="(minimum(2, [AdoMet_dc, Spermine_synt_N,
                     Spermine_synth]) or (Orn_DAP_Arg_deC and Sacchrp_dh_C))"
                     /tool="gutsmash"
     CDS             complement(3..323)
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) CN_hydrolase"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg208_1"
                     /sec_met_domain="CN_hydrolase (E-value: 5.7e-18, bitscore:
                     54.0, seeds: 52, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRNVTVAAVQMKCSKSVEKNIAHAEELVRQAAAKGAEIVLLPELF
                     ERPYFCQERRYEYYEYAQTVEENPAVRHFSRVAAELGIVIPVSFYEKEVNNTYNSVAVL
                     DAD"
     CDS             complement(280..483)
                     /locus_tag="ctg208_2"
                     /transl_table=11
                     /translation="LLAVEIFTVSPSRFPEKISDPDAENFESHVFEGYYLKVMKKLTYV
                     NRSRRNRQDEKCNSGSSSDEMQ"
     CDS             complement(425..1615)
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PAD_porph"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg208_3"
                     /sec_met_domain="PAD_porph (E-value: 2.5e-128, bitscore:
                     416.7, seeds: 436, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="VRLSDISDTKTLRAGFPDICRAIKEKETDMEVTEKNRTKYRMPGE
                     FEPHEGCVMIWPERPGSWNYGAREAQKAFVKVAEAIGVSEKVYMLVSKAQMENAKNQLG
                     NVSGVTLLECETDDAWARDVGATMVLDEKGAVCGVDWQFNAWGGDFDGLYRNWEKDDRV
                     AAFICRTLGCPCLDARPFVLEGGSIHSDGEGTLIVTEACLLSQGRNPQMSREQIEEQLK
                     YWLGVHKIIWLPCGIYQDETNEHVDNVCAFVRPGEVVLAWTEDETDPQYAMSLADLKVL
                     EQETDAKGRKFKIHKLPIPQKTVCLTREDVDGFVFEEGEDERETGERMAASYVNFYISN
                     GGIILPQFGDENDKRAVEILQKCFPERRIYPIDARAIIVGGGNIHCITQQIPGKNI"
     CDS             complement(1563..2684)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Putrescine2spermidine: Orn_DAP_Arg_deC"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg208_4"
                     /sec_met_domain="Orn_DAP_Arg_deC (E-value: 1.4e-15,
                     bitscore: 46.4, seeds: 500, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKIDQLQTPCYVIDEKKMRENLEILKKVQEDTGCKILLAQKAFSG
                     FALYPMIGKYLAGTTASGLFEARLGYEEMGKENHVFSPAYRTEDIEELDRICDHIIFNS
                     TAQLKKFKEVCTRASLGLRINPECSTQGDHAIYDPCAPGSRLGVTRENFDEECLKWIDG
                     LHFHTLCEQNADDLEKTLQAVEEKFGEFLSEVSWLNMGGGHHITREDYDVKLLEKCIQH
                     MKETYDLDIYLEPGEAVALNAGYLVTEVMDIVENEIRTLILDASAACHMPDVLEMPYRP
                     PLKDSGEAGEKAYTYRLSSCTCLAGDVIGDYSFDREIRIGDKLYFMDMAIYSMVKNNTF
                     NGMPLPDIAVMHEDGECEVIRHFGYEDFKSRLS"
     CDS             complement(2674..3954)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Putrescine2spermidine: Sacchrp_dh_C"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Sacchrp_dh_NADP"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg208_5"
                     /sec_met_domain="Sacchrp_dh_C (E-value: 2.7e-44, bitscore:
                     141.1, seeds: 375, tool: rule-based-clusters)"
                     /sec_met_domain="Sacchrp_dh_NADP (E-value: 3.2e-36,
                     bitscore: 113.4, seeds: 81, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSRLLVIGCGGVASVAIRKCCQNSDVFTEIMIASRTKEKCDALKE
                     KIESTTKTKIETAKIDADNAAEVAELIRAYKPDAVLNVALPYQDLTIMDACLEAGADYI
                     DTANYEAEDTEDPTWRAIYEKRCKEKGFTAYFDYSWQWDYDEKFKKAGLTALLGTGFDP
                     GVTSVFSAYALKHYFDEIHTIDILDCNGGDHGYPFATNFNPEINLREVSANGSYWEDGH
                     WVETEPMEFKSVYDFPEVGKKDMYLLHHEEIESLAKNIPGVQRIRFFMTFGQSYLTHMK
                     CLENVGMLSTAPVEFNGQEIVPIQFLKALLPDPASLGPRTVGKTNIGCIFTGVKDGKEK
                     SIYIYNVCDHQECYKEVESQAISYTTGVPAMIGSMMVVTGQWKKPGVFNVEEFDPDPYM
                     EALNKWGLPWKVCEDPELVKVWKANED"
     CDS             complement(3951..4811)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Putrescine2spermidine: Spermine_synth"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Putrescine2spermidine: Spermine_synt_N"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg208_6"
                     /sec_met_domain="Spermine_synth (E-value: 2.2e-54,
                     bitscore: 172.6, seeds: 15, tool: rule-based-clusters)"
                     /sec_met_domain="Spermine_synt_N (E-value: 3.2e-16,
                     bitscore: 48.0, seeds: 13, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MELWYSEFHTGNVKLSVRINRQLFSGESEFQRIDVFESEEFGRFV
                     ALDGEIVFSDKDEFIYDEMVTHVPMTVHPNVKNVLIIGGGDGGVARELIHYPQIESIDV
                     VESDKMFVDVCAEMFPDIAQGLKDERVNIYYEDGLRFLRNKKARYDLIINDSTDPLGHT
                     EGLFTKEFYGSCYKALRDDGIMVYQHGSPFYDEDEVECRKMHRKVFRSFPVSRVYQAHI
                     PTCPSGYWLFGFASKKYHPLEDFRPERFDNLNIETWYYTTNLHRGAFMLPKYVEDLLEE
                     EENSL"
     CDS             complement(4814..6271)
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) OKR_DC_1_C"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) OKR_DC_1_7_arginine"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg208_7"
                     /sec_met_domain="OKR_DC_1_C (E-value: 3.1e-14, bitscore:
                     42.4, seeds: 113, tool: rule-based-clusters)"
                     /sec_met_domain="OKR_DC_1_7_arginine (E-value: 3.9e-127,
                     bitscore: 413.0, seeds: 39, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDYERQQHAPIYEALERFRKKRVVPFDVPGHKRGRGNPELAQLLG
                     EKCVGLDVNSMKPLDNLCHPVSVIREAEELAADAFGAAHAFLMVGGTTSAVQSMILSVC
                     KAGDKIILPRNVHKSAINALVLCGAIPVYVNPEVNAQLGISLGMEVSQVEKAMDENPDA
                     VAVLVNNPTYYGICSDLRTIVKKAHARGMKVLADEAHGTHLYFGRNLPVSGMAAGADMA
                     AVSMHKSGGSLTQSSLLLLNKSMNAEKVRQIINLTQTTSASYLLLSSLDISRRNLALRG
                     EESFEKVAGMAQYAREEINEIGGYYAYGMDLINGTSVFDFDVTKLSIYTLGNGLAGIEV
                     YDLLRDEYDIQIEFGDICNILAYISIGDRLQDIERLVGALADIERLYKKDSTGMLSGEY
                     IAPAVVASPQQAFYAEKESLPMEQTAGRISGEFVMCYPPGIPILAPGEMVTQEIVEYIL
                     YARDKGCSMQGMEDPKVEHLQVLKGGI"
ORIGIN
        1 ccatctgcat ccagaaccgc aacgctgtta taagtattat tgacctcttt ttcataaaag
       61 ctgaccggga tcacaatccc cagctctgcc gctactctgc tgaaatgacg cactgcaggg
      121 ttttcctcta cagtctgcgc atattcataa tactcatatc tgcgctcctg gcagaaatag
      181 ggccgttcaa aaagctccgg aagaagaaca atctcagctc cctttgctgc tgcctgacgc
      241 accagttcct ctgcatgggc aatatttttc tccacacttt tactgcattt catctgaact
      301 gctgccactg ttacatttct catcctgtct gttcctcctg ctcctgttta cataagtaag
      361 ttttttcata actttcaggt aataaccctc aaacacatga ctctcaaagt tttctgcatc
      421 agggtcagat attttttccg ggaatctgct gggtgataca gtgaatattt ccaccgccaa
      481 caatgatggc tctggcatca atgggataaa tccttctttc aggaaaacac ttctgcagaa
      541 tctccactgc ccttttgtca ttttcatccc caaattgtgg aaggatgatc cctccattgg
      601 aaatatagaa attcacgtaa ctggcggcca tccgttctcc ggtttcccgt tcgtcttctc
      661 cctcttcaaa cacaaaacca tccacatctt cccgggtaag gcagaccgtt ttctgaggaa
      721 tcggaagctt atgaatctta aattttctgc cttttgcatc tgtttcctgt tccagcacct
      781 tcagatcagc cagagacatg gcatactgcg gatctgtctc atcctctgtc catgcaagca
      841 caacctctcc gggccttaca aaagcgcaca cgttatccac atgctcattg gtttcatcct
      901 gatagattcc acatggaagc cagataatct tgtggacacc gagccagtac ttcaactgtt
      961 cctctatctg ttccctgctc atctgaggat tgcgtccctg actcagaaga caggcctctg
     1021 taacgatcag agtcccctct ccgtcagaat ggatggaacc gccctcaagc acaaaaggtc
     1081 tggcatccag acatggacaa ccaagagtcc tgcagataaa ggcagccacc ctgtcgtcct
     1141 tctcccagtt ccggtaaagt ccatcaaagt ctcctcccca ggcattaaac tgccagtcaa
     1201 ctccacagac cgcccctttc tcatccagaa ccatggttgc tcccacatct ctcgcccagg
     1261 catcatctgt ctcgcattcc agcaaagtca caccggaaac attcccaagc tgattctttg
     1321 cattctccat ctgagctttg gaaaccagca tatacacctt ttcgctcact ccaatagcct
     1381 ctgcaacttt cacaaatgct ttctgtgctt cccttgcccc ataattccag gaaccgggac
     1441 gttccggcca gatcatcaca catccctcat gaggctcaaa ttctccgggc atacgatatt
     1501 ttgttctgtt cttctctgta acttccatgt ctgtctcctt ttctttgata gcccggcaga
     1561 tatcaggaaa gccggctctt aaagtcttcg tatccgaaat gtctgataac ctcacactct
     1621 ccgtcctcat gcataaccgc aatatccggc agcggcatac cgttaaatgt attattcttc
     1681 accatagaat aaatcgccat atccataaaa tacagcttgt ccccgatccg gatctctctg
     1741 tcaaaggaat aatctccgat cacatctcct gccaggcagg tgcaggaaga taagcggtag
     1801 gtatatgcct tctcccctgc ttccccgctg tccttaagag gcggacgata gggcatctcc
     1861 agcacatccg gcatatggca ggctgcagaa gcatccagaa tcagcgttct gatctcattt
     1921 tccacaatgt ccataacctc tgtcaccaga tatccggcat tcagggcaac agcctccccg
     1981 ggctccagat aaatatccag atcataggtt tccttcatat gctgaataca tttttccagg
     2041 agctttacat cataatcctc tctggtaata tgatgtccgc cgcccatatt cagccaggaa
     2101 acctcagaca gaaattctcc aaacttctcc tctaccgcct gcaatgtttt ctccaggtca
     2161 tccgcattct gttcgcagag cgtatgaaaa tgcagtccgt cgatccattt cagacattcc
     2221 tcgtcaaagt tctctctggt aacccccagc ctggaacccg gtgcacaggg atcatagatt
     2281 gcatgatccc cctgagtgga gcattccgga ttaatacgga gtcccagact ggctctggta
     2341 cacacctctt taaatttctt cagctgtgct gtggaattaa agataatatg gtcacagata
     2401 cgatccagtt cctctatatc ttctgtacgg taagcagggg aaaaaacatg attctctttc
     2461 cccatctctt catatccaag ccttgcctca aacagaccgc tggcagtggt acctgccaga
     2521 tattttccga tcatgggata cagagcaaag ccggaaaacg ctttctgcgc cagaaggatc
     2581 ttacacccgg tatcctcctg gacttttttc aaaatctcca gattctcccg catcttcttc
     2641 tcatcgatca catagcaggg agtttgcagt tgatcaatct tcattggctt tccagacctt
     2701 taccagttca ggatcttcgc agactttcca tggaagtccc catttgttca gtgcttccat
     2761 atatggatcc ggatcaaatt cttccacatt aaatacaccc ggcttcttcc actgtccggt
     2821 cactaccatc atggaaccga tcatagccgg aacacctgtt gtataggaga ttgcctggga
     2881 ttccacctct ttatagcatt cctgatgatc acatacatta taaatataaa tacttttctc
     2941 ttttccgtcc ttaacacctg tgaaaataca gccgatattt gttttaccca ctgttctcgg
     3001 acccagagat gcaggatccg gcagaagagc tttcaaaaac tggatcggca cgatctcctg
     3061 tccgttaaat tccacaggtg cggtggaaag cattcctaca ttttccagac atttcatatg
     3121 tgtcagatag ctctgtccga atgtcataaa gaaacggatt ctctgtactc ccggaatatt
     3181 ctttgccagt gattcaatct cttcatgatg aagaagatac atatccttct ttcctacctc
     3241 agggaaatcg tagacagatt taaattccat aggctctgtt tctacccaat gtccgtcttc
     3301 ccagtaggaa ccatttgcgg aaacctctcg aagattgatc tccgggttaa agtttgtggc
     3361 aaaaggatat ccatgatcac cgccgttaca gtccagaata tcaatggtat ggatttcatc
     3421 aaaataatgc ttcagggcat aagcactgaa tacactggta acacccggat caaaaccggt
     3481 tcccagaaga gcagtcagtc ctgctttctt aaatttctca tcataatccc actgccagga
     3541 ataatcgaaa tatgcagtaa agcctttttc tttacatctc ttttcataga ttgcacgcca
     3601 tgtgggatct tctgtatcct ctgcttcata attggcagta tcaatataat ccgcaccggc
     3661 ttccagacat gcatccatga tggtaagatc ctgatatgga agtgccacat tcaggacagc
     3721 atccggctta taggctctga ttaattctgc gacctctgct gcattatccg catctatctt
     3781 tgctgtctca atttttgtct tcgttgtact ttcaatcttc tctttgagtg catcgcattt
     3841 ttcctttgtc ctgcttgcga tcataatctc tgtaaaaaca tcgctgttct gacagcattt
     3901 gcgaattgcc actgaagcta ccccaccaca tccgattacc aataatctgc tcataaactg
     3961 ttttcctcct cttccagtaa atcttctaca tattttggaa gcataaatgc tcctctgtga
     4021 aggtttgttg tataatacca tgtttctata ttaagattat caaacctctc cggcctgaaa
     4081 tcttccagtg gatgatattt ctttgatgca aatccaaaaa gccagtatcc cgagggacag
     4141 gtagggatat gtgcctgata aaccctgctc accggaaatg accggaaaac ctttctgtgc
     4201 atctttctac attccacctc atcctcatca taaaaaggac ttccatgctg ataaaccatg
     4261 attccgtcat ctctgagagc cttataacag cttccgtaga attcctttgt aaaaagtccc
     4321 tctgtatgtc ccagcggatc tgtggaatca ttgatgatca ggtcatatct ggccttttta
     4381 ttccgcagga accgaaggcc gtcttcataa taaatgttga ctctctcatc cttcagtccc
     4441 tgggcaatat ccggaaacat ctccgcacat acatccacaa acattttatc ggattccacc
     4501 acatcaatgc tctcaatctg tggataatgg atcagttctc tggcaactcc accgtcaccg
     4561 ccgccgatga tcagaacatt ttttacattg ggatgtactg tcattggcac atgcgtgacc
     4621 atttcatcat aaataaattc atccttgtca gaaaatacga tctcaccgtc cagagccaca
     4681 aatcttccaa attcttcaga ctcaaacaca tcgattctct gaaattcact ttcccctgaa
     4741 aataactgtc ggttaatccg gacagaaagc tttacatttc ctgtatggaa ctcggaatac
     4801 cataattcca tgatcagata ccccctttca ggacctgaag atgttctacc ttcggatcct
     4861 ccattccctg catggaacag cctttgtctc ttgcataaag aatgtattct acaatttcct
     4921 gtgtcaccat ttccccagga gcaagaatcg ggattcccgg aggataacac ataacaaatt
     4981 caccgctgat cctgcctgcc gtctgttcca tgggcagaga ttctttctcc gcataaaatg
     5041 cctgctgtgg agacgccacc actgcaggtg cgatatattc cccggaaagc atacctgtgc
     5101 tgtccttctt atacagacgt tcaatatcag ccaatgcccc taccagtctc tcaatatcct
     5161 gaagcctgtc tccgatggaa atgtaggcca gaatattaca gatatctcca aactcgatct
     5221 gaatatcata ttcatctctg agaagatcat aaacttcaat acctgccagc ccatttccca
     5281 atgtataaat ggagagtttt gtaacatcaa aatcaaaaac actggttcca ttgatcagat
     5341 ccataccata tgcataatag ccgccgatct cattgatctc ctctctggca tactgcgcca
     5401 tccctgcaac cttttcaaag gattcctctc ccctgagtgc cagatttctt ctggagatat
     5461 ccagactgga caacagaaga taagaagcac ttgtggtctg agtcagattg atgatctggc
     5521 gcactttctc tgcattcata ctcttattca aaagcagcag cgaactctgt gtcagacttc
     5581 cgccggattt gtgcatggaa actgccgcca tgtcggcacc tgccgccatt ccggatacag
     5641 gaaggtttct gccgaaataa agatgggttc catgagcctc atctgccagc accttcattc
     5701 ctctggcatg agccttcttt acgatagtcc gcaaatccga acagattcca taataggtag
     5761 ggttatttac aagaaccgcc accgcatccg ggttctcatc catagccttc tccacctggc
     5821 tgacctccat gccaagggag atacccagct gcgcattcac ctccgggttt acataaaccg
     5881 gaattgcacc acacagtacc agggcattga tggcactttt atgtacattt cgcggcagaa
     5941 tgatcttgtc ccctgcctta cagacagaaa ggatcatact ctgcactgca gatgtggttc
     6001 ctcctaccat aagaaatgca tgcgctgcgc caaaagcatc tgctgcaagc tcctccgctt
     6061 ctctgatcac cgaaaccggg tggcagagat tgtccagcgg cttcatagaa ttaacatcca
     6121 gccccacaca tttctctccc agcaactgtg caagctccgg atttcctctt cctcttttat
     6181 gtcccggcac atcaaaggga acgactctct tctttcggaa acgttccagt gcttcatata
     6241 tgggcgcatg ctgttgtctt tcataatcca tttgacatgt tcctcttttc ctgtcgtcat
     6301 gtaccggcaa atacagttct atcattcagc aattgtatac tgcctcaaac cggcacaatg
     6361 attcaattat ttct
//
